Input and runtime details for job 4935127 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_003197
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 4935127 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:12:23 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:14:40 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:16:11 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:17:25 CET 2020
	Job Completed		@ Mon Jan 27 14:17:35 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=4935127&toolName=IntaRNA ( 30 days expiry )

Description of the job

GcvB - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
STM3083	-36	-6	-13	-7	4.19	GcvB	130	160	131	137	6.26	-15.04	-25.49	-1.45822	CAGAAUUGGUAAACAAAACAGGAGUGGGAAA&UUUUUCACUUCCUGUACAUUUACCCUGUCUG	((((...((((((....((((((((((((((&)))))))).))))))...))))))...))))	AGUGUGAUCAUAUUCACGGAUUUUGGUAUGGUGUUUAAGCAGAAUUGGUAAACAAAACAGGAGUGGGAAAAAUUCAUGGACACCAUAGCUCGCCAGUUAGACGCCGCAAGGCACCAAUUCAAAACGACUUAUUCACGUCAGGGAAUGGAA	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.009252	0.7883581		1254606	fructuronate reductase
STM1033	42	70	60	66	5.67	GcvB	132	162	137	143	7.38	-15.02	-28.07	-1.45628	GACAGCAGGGAACGGACGCGGGGAGGGAA&UUUCACUUCCUGUACAUUUACCCUGUCUGUC	((((((((((........(((((((((((&)))).)))))))........))))).)))))	ACGAAAACCGUAAGCUGGGACUGGUAUUUGAUACUGAUCCGGCACAUGACCCGGGGGAACAGGAUAAUGCCGGAAGUGAAGACAACAGUGGCGGCGAUAAAAAUGCCGCCGGUGAUGACAGCAGGGAACGGACGCGGGGAGGGAAGCAGU	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0093682	0.7883581		1252551	Gifsy-2 prophage Clp protease-like protein
STM0085	63	75	69	75	2.29	GcvB	152	164	152	158	1.44	-14.92	-18.65	-1.44659	UGAACAGGCAGGG&CCCUGUCUGUCCA	((.((((((((((&)))))))))).))	CAAUAAUAUUCACUAUUAGCCUGGCGAAUUUGUCACUACACUUUGUAAUAAAAAACAGAUCAUCACAGGCUAAUCAUGAUCCUCAUUAUUUAUGCGCAUCCGUAUCCGCAUCAUUCUCAUGCGAAUAAGCGGAUGCUUGAACAGGCAGGG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.009969	0.7883581	yabF	1251603	glutathione-regulated potassium-efflux system ancillary protein KefF
STM0412	27	72	27	33	5.33	GcvB	35	73	67	73	10.95	-14.92	-31.2	-1.44659	ACAUUACUUCCGGCGAUGCCACCAGCCUAAAAACAGACCUGUCGCA&UGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGU	(((((((..((......((((..((((((....((((.....((((&))))...)))).))))))..))))....))..)))))))	GUCGUGGGUUACAACUUCUAAACCUGCAAAAAAGGCCAGAGGUCGUCCUCUGGCCCGUUUCGGGCGCAUGCUGCCAUGCGGCGGCGAGUCGCGCCCACUUCACAUUACUUCCGGCGAUGCCACCAGCCUAAAAACAGACCUGUCGCAAUC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.009969	0.7883581		2673775	putative inner membrane protein
STM0680	-43	-18	-25	-19	4.75	GcvB	71	90	72	78	4.12	-14.91	-23.78	-1.44562	GCAAAUACAAAAAGUACGACGCAACA&UGUUGUGUUGUUGUGUUUGC	(((((((((......(((((((((((&))))))))))))))))))))	CCUUCCACGCCUUUGCGGCAUUUUUUAGUCAAGCAAAUACAAAAAGUACGACGCAACAGAUUAACGGAGAAGGCUAUGUGUUCUAUUUUCGGCGUAUUCGAUAUCAAAACAGAUGCAGCGGAACUGCGUAAAAAAGCCCUUGAACUGUCA	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0100309	0.7883581	asnB	1252200	asparagine synthetase B
STM3843	7	49	43	49	16.07	GcvB	37	85	37	43	13.09	-14.9	-44.06	-1.44465	CAUAACGACACUAUCGUCGCCCAGGCAACCCCACCGGGACGCG&CGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUG	(((((((((((...((((((((((.(((.((((.(((((((((&))))))))).))))...))).))...))..))))))..)))))))))))	AACGCUUAUCAGGCCUACAAUCAAAAGGCGGUCAUAUGACCGCCUUUUUUUAUUGCAACAAAGUUGAGACUAACCAUGAGCCAUAACGACACUAUCGUCGCCCAGGCAACCCCACCGGGACGCGGUGGCGUGGGUAUCCUGCGUAUUUCC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0100932	0.7883581	trmE	1255370	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE
STM0069	-71	-52	-71	-65	3.48	GcvB	60	81	75	81	6.88	-14.87	-25.23	-1.44174	ACAGCACAUGCGCAACCGUA&UACGGUUGUGAUGUUGUGUUGU	((((((((..((((((((((&))))))))))....))))))))	GCCUACAGCACAUGCGCAACCGUAGACCGGAUAAGGUGUUUACGCCGCCAUCCGGCAAAAAACCGAGGUACAACUAUGAGCUAUUACGCCUUUGAAGGACUGAUCCCGGUGGUGCAUCCGGACGCGUUUGUCCACCCCAGCGCCGUGCUG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0102821	0.7883581	caiE	1251587	phenylacetic acid degradation protein PaaY
STM1282	56	70	64	70	2.38	GcvB	36	50	36	42	5.56	-14.86	-22.8	-1.44077	CCCAGGAGAACGCGC&GCGUGUUCUGAUGGG	((((..(((((((((&)))))))))..))))	AACAAAAACAACAUCGUUCAUCAUAGUGCUUCGCAGUAAAUGUUCAUAUUGCACCAGGAGUGAGAAAUGGCCGAAAUGACUGAAGUCGCGAAGGGUGUCGCUACACACCAACGUUUAGUGGCUUUAUUAACCCAGGAGAACGCGCGCUAU	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0103458	0.7883581	yeaK	1252800	putative cytoplasmic protein
STM3527	-66	-47	-54	-48	2.5	GcvB	139	163	140	146	4.45	-14.85	-21.8	-1.4398	GGACAACAGUUGGUGCAGGA&UCCUGUACAUUUACCCUGUCUGUCC	(((((((((...((((((((&)))))))).......)))).)))))	AGCCCGCAGGGACAACAGUUGGUGCAGGAUGUGGGAUAUGUGCCGCUGUAUCCGGCCGCGAAAUAAAGGGACUAAAUGGAAGAGGUUAAAUUAUAUGUGGAACGGGUUGGCAUAGACCCGGCCAGCGGCGCUGAUAUUCGUAUUGCACGG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0104098	0.7883581		1255050	similar to E. coli orf hypothetical protein (AAC73602.1) Blastp hit to AAC73602.1 (86 aa) 35% identity in aa 21 - 85
STM4558	-7	58	24	30	12.74	GcvB	36	91	63	69	14.51	-14.84	-42.09	-1.43883	UGCGAACAUGAACACGAUUUCUAUCCUCAGCACGACCGAUCUCCCCGCAGCCUGGCAGAUUGAGC&GCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCA	(((((((((((((((((..........((.((((((((.....((...((((...((((....((&))....))))...))))...))....)))))))).))))))))..)))))))))))	UCACGACGCCGGCGUUUAAUGAACUCCGGGCAAACCCGGCGGCACGCGCCGCACUAUGGCAACAAAUCUGCGAACAUGAACACGAUUUCUAUCCUCAGCACGACCGAUCUCCCCGCAGCCUGGCAGAUUGAGCAACGCGCUCAUGCGUUU	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0104742	0.7883581	rimI	1256084	ribosomal-protein-alanine N-acetyltransferase

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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