Input and runtime details for job 4935127 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_003197
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 4935127 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:12:23 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:14:40 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:16:11 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:17:25 CET 2020
	Job Completed		@ Mon Jan 27 14:17:35 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=4935127&toolName=IntaRNA ( 30 days expiry )

Description of the job

GcvB - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
STM4228	-39	-26	-33	-27	7	GcvB	1	15	2	8	4.12	-14.12	-25.24	-1.36902	UCCGGCCAGGAAGU&ACUUCCUGAGCCGGA	((((((((((((((&)))))))).))))))	CGUAGGCCUGAUAAGCGCAGCGCCAUCAGGCACAUCUCCGGCCAGGAAGUUUCAACGCUGUAGAGGAAAACCCCCAUGGAUGUCAUUAAAAAGAAACACUGGUGGCAAAGCGACCAGCUUAAAUGGUCAGUAAUUGGUCUGCUGGGCCUG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0161607	0.7979576	malF	1255754	maltose ABC transporter permease MalF
STM3720	14	23	14	20	2.2	GcvB	154	163	157	163	1.28	-14.11	-17.59	-1.36805	GGAUAGACAG&CUGUCUGUCC	((((((((((&))))))))))	AAGCCGCUCGAAUUAAAGAAAGGACAAUAAGAAAAAAUCUGUAAUUAUAAUAAUUAUGCACUCAGUGAGUAAUUUAUGAGUGAGACAAGGAUAGACAGAAUGUCUAACAUUAAAAGUUAUAUUUCAAACCAAAAAAACAUAAUUCAACAU	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.016256	0.7979576	yibR	1255244	putative inner membrane protein
STM3984	-15	-3	-10	-4	0.93	GcvB	148	161	149	155	1.2	-14.06	-16.19	-1.3632	ACAGAAGGGUAAA&UUUACCCUGUCUGU	(((((((((((((&)))))))).)))))	GGCAGAGAUCACGCUCUCGUGGGGCGAUGUGCUAAGAUGCGGGAACUUGAGGUCAAAAAAACAGAAGGGUAAAAAAUGGAAUCACUGGCCGCGCUCUAUAAAAAUCAUAUUGUUACCUUACAAGAACGGACGCGCGAUGUUCUGGCGCGC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0167404	0.8089837	pepQ	1255510	Xaa-Pro dipeptidase
STM0804	-70	-36	-55	-49	9.43	GcvB	36	76	53	59	10.8	-14.05	-34.28	-1.36223	AUAUCAUUGCGCCGCAGCUGGAUGCCCGUACACGC&GCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGU	(((.((((((.(((.(((((((.((((((((((((&))))))....)))))).)))))))..)))..)))))).)))	GGAGAAUAUCAUUGCGCCGCAGCUGGAUGCCCGUACACGCCCAUGUAAUUUUCACCCUCAUUUAAAGAAAUAACGAUGUCGCAAUUAACCCAUAUAAACGCCGCAGGCGAAGCGCACAUGGUGGACGUUUCCGCGAAAGCGGAAACCGUU	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0168389	0.8089837	moaC	1252324	molybdenum cofactor biosynthesis protein C
STM3257	-68	-44	-63	-57	1.65	GcvB	46	81	70	76	9.96	-13.98	-25.59	-1.35545	ACAAAACAACAUUACGAAAGCCUAU&AUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGU	((((.((((((((((((((((((((&))))))))...........)))))))))))).))))	CAAAAUCACAAAACAACAUUACGAAAGCCUAUAAAUUCUUUCGAAGAAGAAAAACGAAAGAACUCGGAGAUAAAAGUGAAAGAAAUAAUUGCUCGUCAUAAAGCAGGAGAACAUCUGGGCAUCUGUUCAGUUUGUUCUGCUCAUCCCCUG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0175423	0.8300455		1254780	D-tagatose-16-bisphosphate aldolase subunit GatZ
STM2573	-66	-17	-37	-31	7.72	GcvB	42	84	53	59	12.2	-13.97	-33.89	-1.35448	AUAACGAAAAAAUAUGUCGCAACGGUUUUAGCCAAAAUUCAUUAAUCAGA&UCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGU	(((((((......((((((((((.((...((((((((.......((((((&))))))....)))))))).)).))))))))))....)))))))	UUGACCCUGAUAACGAAAAAAUAUGUCGCAACGGUUUUAGCCAAAAUUCAUUAAUCAGAUAAUAAGGAAAUAAAAAUGAAAAUUGCAAUCGCAGGUGCAGGCGCUAUGGGAUGUCGUUUUGGCUAUAUGCUGCUGGAGGCCGGGCACGAC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0176449	0.8300455		1254095	2-dehydropantoate 2-reductase
STM4312	-24	24	-20	-14	11.29	GcvB	53	90	80	86	9.63	-13.95	-34.87	-1.35254	GCUCACGCAACAAUUAAGCUGACUAUGGAUACACAACCGUCCGCCGAA&UUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGC	((..(((((((((...(((.........((.(((((((((..((((((&))))))..))))))))))))))...)))))))))..))	GCGAUAAUAUCACUAACAUCAAUACACCGACCUUUAUCGGGUCGCUUAGAAGCUCACGCAACAAUUAAGCUGACUAUGGAUACACAACCGUCCGCCGAACCAACGUUGGGAUCGACUGUUAGCAGUCUGGAUAACACCGUCACCCUUAAG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0178517	0.8311163		1255838
STM1257	-35	-12	-32	-26	7.95	GcvB	166	189	180	186	6.29	-13.92	-28.16	-1.34963	UGUGGUGGUGCUUAAUCGAUCGCU&AGUGAUUAAUGUAGCACCGCCAUA	((((((((((((..((..((((((&))))))..))..))))))))))))	CACUAUUAGUGUUUGCCGGAAAUACGAUUUCAGCCUGGCUUGUGGUGGUGCUUAAUCGAUCGCUGGAGCGUCCCGAUGCUCUAUAAUCCUGCCCCGACGUUGUUUCGUCUGCUCCUCUCCGUUACCUGUCUGCUGUUUAUUGCCGGAUAC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.018166	0.8371189		1252775	peptide ABC transporter permease
STM4007	-39	-20	-26	-20	2.82	GcvB	63	85	63	69	7.27	-13.85	-23.94	-1.34284	CACGACGACCGACCAUGACC&GGUUGUGAUGUUGUGUUGUUGUG	((((((((((((((((((((&)))))))..)))).)))))))))	CGGCGACACGGCCAGCAGAAUUGAAGAUCUCGUUACCACGACGACCGACCAUGACCAAUCCGGGAGAGUACAAGUAUGUCCGCUGAACACGUUUUGACGAUGCUGAACGAGCACGAAGUGAAGUUUGUCGAUCUGCGCUUCACCGAUACC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0189185	0.8629894	glnA	1255533	type I glutamate--ammonia ligase
STM4083	-40	-22	-40	-34	2.95	GcvB	138	155	149	155	2.38	-13.8	-19.13	-1.33799	AGGGUAAACUGUAGCGGAA&UUCCUGUACAUUUACCCU	((((((((.((((..((((&))))..))))))))))))	UCACGAUUAAAACGAUAACUGUCGGGCUUGUCAAAAGGGUAAACUGUAGCGGAAGGCAAAAUAUAAGGAACUUACAUGACCAUACAGCAAUGGUUAUUCUCAUUUAAAGGGCGUAUCGGACGCCGUGAUUUCUGGAUUUGGAUCGGCCUC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0194729	0.8669559	yiiR	1255610	similar to E. coli orf hypothetical protein (AAC76903.1) Blastp hit to AAC76903.1 (146 aa) 90% identity in aa 1 - 141

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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