Input and runtime details for job 4935127 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_003197
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 4935127 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:12:23 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:14:40 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:16:11 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:17:25 CET 2020
	Job Completed		@ Mon Jan 27 14:17:35 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=4935127&toolName=IntaRNA ( 30 days expiry )

Description of the job

GcvB - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
STM1787	-64	2	-37	-31	11.4	GcvB	34	90	54	60	15.42	-16.39	-43.21	-1.58911	GUAAACGCCACAAACAACAGUUAGCGCAAGCCGAACAACAGCCGGACAAUGAGGAUAAACAGGCAU&AUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGC	((((((((.((((((((((.....((.((((((((....((((.....((.((....((((.((((&)))).)))))).)).)))))))))))).)).......)))))).)))).))))))))	GCUCAACCAACGUAAACGCCACAAACAACAGUUAGCGCAAGCCGAACAACAGCCGGACAAUGAGGAUAAACAGGCAUGAGUAACCAGUAUCAAACCCAGGGUUAUACCGUUAACGACGCCGGCCGUCGUCUGAUCGUGGACCCGAUUACC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0038311	0.6975035		1253306	[Ni/Fe] hydrogenase large subunit
STM0634	20	62	49	55	5.65	GcvB	35	77	39	45	11	-16.39	-33.04	-1.58911	CAGAAAGACAACCGGCCGAUCAACACCUACAGGACAAACCGCA&UGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUG	((.((..((((((((((((......(((((((((((...((((&))))))))))).))))...)))))...)))))))....)).))	UUAAAACCAACCCAUAAAUGCAAAAUAUACUAUUUCAUCUAUUAUUCAUAAUACGAAUGAAUUCGGAGCCAGAGCGUGAGUGAUAAUAAUCAGGCAGAAAGACAACCGGCCGAUCAACACCUACAGGACAAACCGCAAAUUUUCAGAACA	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0038311	0.6975035	ybeF	1252154	putative LysR family transcriptional regulator
STM1715	-75	-43	-52	-46	2.7	GcvB	42	71	45	51	9.85	-16.36	-28.91	-1.5862	AUCAAUUUCCUGUGAGAAUAUUUGCCCAUCAGA&UCUGAUGGGCUUUUGGCUUACGGUUGUGAU	((((.....(((((((.......((((((((((&))))))))))......)))))))...))))	AUCAAUUUCCUGUGAGAAUAUUUGCCCAUCAGAAGUGCUCAGCGUAGACUAAUCUCCUUGUUUAAGGAGUAAAAAAUGCAUAAAGAGACGCAACCUAUCGAUCGCGAAACACUGUUGCUUGAAGCCAACAAAAUUAUUCGGGAACAUGAA	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0039103	0.6975035	yciN	1253234	similar to E. coli orf hypothetical protein (AAC74355.1) Blastp hit to AAC74355.1 (83 aa) 92% identity in aa 1 - 83
STM3495	52	64	57	63	3.25	GcvB	181	193	182	188	2.72	-16.35	-22.32	-1.58523	GCAAUAUGGCGGU&ACCGCCAUAUUGC	(((((((((((((&)))))))))))))	GCCAACCACAGAAAAAUGUUUAUUGGACAGGGUGCGACUGACCACGCCUGACAGACUUAGUAAGAUGGGGAAAGCAUGAGCACCAUUCUGAUUUUUAUAGCUGCUUUGCUGGCCUGCUCUCUACUGGCAAUAUGGCGGUUCCGGGUAAAG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0039371	0.6975035	yrfF	1255018	Intracellular growth attenuator protein igaA
STM2909	41	74	68	74	7.52	GcvB	47	83	47	53	11.67	-16.31	-35.5	-1.58135	CGAUGAUGCAGCAGUAUCUCAAGCUGAAAGCCCA&UGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUG	(((((((((((((..(((..((((((((((((((&))))))))))))))..))).....)))))))))))))	UUUAUGUGAGUUUAUAAGGGUUUUACCUGGGGCCACAUGAGCACUUGAAAGAAUAAAAAAACGAGUAAAAUCAAUAUGAAUGAGUCAUUUGAUAAGGACUUCUCCAACCACACCCCGAUGAUGCAGCAGUAUCUCAAGCUGAAAGCCCAG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0040457	0.6975035	mutS	1254432	DNA mismatch repair protein MutS
STM1498	-54	-38	-48	-42	4.66	GcvB	76	92	80	86	3.94	-16.29	-24.89	-1.57942	UUGCGAACACAACGACA&UGUUGUUGUGUUUGCAA	(((((((((((((((((&)))))))))))))))))	ACGCGCCCGGAGCUCCCCGUUUUGCGAACACAACGACAUUUAAAACUCUUUUUUGGAUGAGCAUAGUGAGCUGUUAUGAAAAUCACCAACCCUGAGGCAUUAAUGGCGGCCAGUCUCAGCCGACGAAGCCUGGUGAAAACCUCCGCUAUC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0041011	0.6975035		1253016	dimethyl sulfoxide reductase subunit A
STM0689	-9	15	5	11	2.15	GcvB	66	90	71	77	6.6	-16.13	-24.88	-1.5639	GCAAACCCUAUGGCACAACACACA&UGUGAUGUUGUGUUGUUGUGUUUGC	((((((...(((((((((((((((&)))).)))))))))))...))))))	ACAUGCGGGGGAUACAGGCAACUGACCCGCAACAUCUUACCCAUAAAACAAUAAAGACAGUGGAGAGCAAACCCUAUGGCACAACACACACCUGCAACAUCGCGCGCCGGCACGUUCGGCGCAAUACUGCGCGUGACAAGCGGCAAUUUC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0045687	0.6975035	citA	1252209	citrate-proton symporter
STM2355	-57	-39	-48	-42	3.35	GcvB	72	91	75	81	4.11	-16.13	-23.59	-1.5639	UGCAAACACACAACGCCAC&GUUGUGUUGUUGUGUUUGCA	((((((((((((((((.((&)).))))))).)))))))))	GACCUGCAUGAAAAAGUCUGCAAACACACAACGCCACGUAAAACAUAAGAAAAUGACGCCACUUGAGGGGUAUGUAUGAAGAAGACCGUUCUCGCUUUGUCUUUGCUGAUAGGUCUGGGCGCGACGGCGGCCAGUUACGCCGCGCUCCCG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0045687	0.6975035	argT	1253877	histidine ABC transporter substrate-binding protein HisJ
STM3089	56	73	67	73	1.87	GcvB	157	174	157	163	3.11	-16.07	-21.05	-1.55809	UUAAUCAGUAUGGAUGGA&UCUGUCCAUAGUGAUUAA	(((((((.((((((((((&)))))))))).)))))))	GUAAAAAGGUGUUUUGCCAUAUUUAAUUUCACCUAAGGAGAAUUUAGUUAGCUCAAACUGUUGUGCGGAAUAGUCAUGACAGAAGAUUCUACCAUGAGUCUGAAGGUUGCUGACACUGGCAGUCUGAGUGUUAAUCAGUAUGGAUGGAUU	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0047561	0.6975035	yqgD	1254612	similar to E. coli orf hypothetical protein (AAC75978.1) Blastp hit to AAC75978.1 (83 aa) 52% identity in aa 1 - 78
STM1179	22	44	38	44	7.1	GcvB	35	58	35	41	5.79	-16.06	-28.95	-1.55712	GCCAAAACCGGUCUGGACGCGCA&UGCGUGUUCUGAUGGGCUUUUGGC	(((((((((..((.(((((((((&))))))))).))..)).)))))))	AGGUUAUCACCAGCGUAGAUGAGAACGAAGGGCGAGCUAACCAACUGCUGUCGAUGAGUUAAUACAGGACAUUUUAUGAUCAGUUCAUUAUGGAUCGCCAAAACCGGUCUGGACGCGCAGCAAACCAAUAUGGAUGUGAUUGCCAAUAAC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.004788	0.6975035	flgG	1252697	flagellar basal-body rod protein FlgG

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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