Input and runtime details for job 8257533 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 8257533 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:34:31 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:34:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:30:38 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:30:45 CET 2018
	Job Completed		@ Fri Feb 16 22:31:01 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=8257533&toolName=CopraRNA ( 30 days expiry )

Description of the job

LhrA2

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003210 Listeria monocytogenes EGD-e

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
24	0.004153	0.4656	lmo1386	N/A	-22.14	0.003328	2 -- 37	155 -- 186	DNA translocase	_____________1____________________________________38\n_____________\|____________________________________\|\n__________5'-G___G________G__AAGCU______CAC_______CCGCA...UUA-3'\n______________GAA_UACGCGGC_GA_____GAUGAA___GAAAACA\n______________\|\|\|_\|\|\|:\|\|\|\|_:\|_____\|\|\|\|\|\|___\|\|\|\|\|\|\|\n______________CUU_AUGUGCCG_UU_____CUACUU___CUUUUGU\n3'-GUU...CUGUA___G________G__ACU________A_________UUAUU...GGA-5'\n_____________\|____________________________________\|\n_____________187__________________________________154\n	31 -- 37	155 -- 161	-32.0969 kcal/mol	6.67763 kcal/mol	9.66922 kcal/mol	1 38@LB@ \| \|@LB@ 5'-G G G AAGCU CAC CCGCA...UUA-3'@LB@ GAA UACGCGGC GA GAUGAA GAAAACA@LB@ \|\|\| \|\|\|:\|\|\|\| :\| \|\|\|\|\|\| \|\|\|\|\|\|\|@LB@ CUU AUGUGCCG UU CUACUU CUUUUGU@LB@3'-GUU...CUGUA G G ACU A UUAUU...GGA-5'@LB@ \| \|@LB@ 187 154@LB@@LB@@LB@Energy -22.14 kcal/mol@LB@Hybridization Energy -32.0969 kcal/mol@LB@Unfolding Energy - mRNA 6.67763 kcal/mol@LB@Unfolding Energy - sRNA 9.66922 kcal/mol@LB@@LB@Position - mRNA 2 -- 37@LB@Position - sRNA 155 -- 186@LB@Position Seed - mRNA 31 -- 37@LB@Position Seed - sRNA 155 -- 161@LB@
34	0.007076	0.552	lmo2678	N/A	-19.30	0.013995	184 -- 246	155 -- 207	XRE family transcriptional regulator	_____________183__________________________________________________________247\n_____________\|____________________________________________________________\|\n5'-GAA...AGACG____AG_____AA______A__AA______U___CUAAUUGUGGA__________U____UCAGU...AAG-3'\n______________GAGG__AUGGA__AUGAAC_GC__GCGGCU_GUG___________AGAUGAA_GA_GGCA\n______________\|\|\|\|__\|\|:\|\|__\|\|\|\|\|\|_:\|__:\|\|\|\|:_:\|\|___________\|\|\|\|\|\|\|_\|\|_::\|\|\n______________CUCC__UAUCU__UACUUG_UG__UGCCGG_UAC___________UCUACUU_CU_UUGU\n3'-GUU...CCCUA____G______G_______A__________U_____________________A__U____UUAUU...GGA-5'\n_____________\|____________________________________________________________\|\n_____________208__________________________________________________________154\n	233 -- 239	163 -- 169	-37.3867 kcal/mol	12.1272 kcal/mol	13.9822 kcal/mol	183 247@LB@ \| \|@LB@5'-GAA...AGACG AG AA A AA U CUAAUUGUGGA U UCAGU...AAG-3'@LB@ GAGG AUGGA AUGAAC GC GCGGCU GUG AGAUGAA GA GGCA@LB@ \|\|\|\| \|\|:\|\| \|\|\|\|\|\| :\| :\|\|\|\|: :\|\| \|\|\|\|\|\|\| \|\| ::\|\|@LB@ CUCC UAUCU UACUUG UG UGCCGG UAC UCUACUU CU UUGU@LB@3'-GUU...CCCUA G G A U A U UUAUU...GGA-5'@LB@ \| \|@LB@ 208 154@LB@@LB@@LB@Energy -19.30 kcal/mol@LB@Hybridization Energy -37.3867 kcal/mol@LB@Unfolding Energy - mRNA 12.1272 kcal/mol@LB@Unfolding Energy - sRNA 13.9822 kcal/mol@LB@@LB@Position - mRNA 184 -- 246@LB@Position - sRNA 155 -- 207@LB@Position Seed - mRNA 233 -- 239@LB@Position Seed - sRNA 163 -- 169@LB@
42	0.009182	0.5883	lmo1767	purM	-18.99	0.016282	211 -- 267	155 -- 213	phosphoribosylaminoimidazole synthetase	_____________210__________________________________________________________________________268\n_____________\|____________________________________________________________________________\|\n5'-GGA...ACAAA___AAU___A_AAA_______UA_UAAA_C____________A______________UCA__U_________C___UCAAA...UAG-3'\n______________UCG___GGC_G___AUGCAUA__G____G_AGGCGUGGACGU_GAA__GC_UGGCUA___AG___AGUGGAA_GCA\n______________\|\|\|___\|\|\|_\|___\|\|\|\|\|\|\|__\|____\|_\|\|\|\|:\|:\|\|\|:\|_\|\|\|__:\|_:\|\|\|:\|___\|\|___\|:\|\|:\|\|_:\|\|\n______________AGC___CCG_C___UACGUAU__C____C_UCCGUAUCUGUA_CUU__UG_GCCGGU___UC___UUACUUU_UGU\n3'-GUU...GUACA___________GGA_______UG_CCUA__________________GA__U______UAC__UAC___________UUAUU...GGA-5'\n_____________\|____________________________________________________________________________\|\n_____________214__________________________________________________________________________154\n	257 -- 263	158 -- 164	-45.9009 kcal/mol	14.0255 kcal/mol	22.2914 kcal/mol	210 268@LB@ \| \|@LB@5'-GGA...ACAAA AAU A AAA UA UAAA C A UCA U C UCAAA...UAG-3'@LB@ UCG GGC G AUGCAUA G G AGGCGUGGACGU GAA GC UGGCUA AG AGUGGAA GCA@LB@ \|\|\| \|\|\| \| \|\|\|\|\|\|\| \| \| \|\|\|\|:\|:\|\|\|:\| \|\|\| :\| :\|\|\|:\| \|\| \|:\|\|:\|\| :\|\|@LB@ AGC CCG C UACGUAU C C UCCGUAUCUGUA CUU UG GCCGGU UC UUACUUU UGU@LB@3'-GUU...GUACA GGA UG CCUA GA U UAC UAC UUAUU...GGA-5'@LB@ \| \|@LB@ 214 154@LB@@LB@@LB@Energy -18.99 kcal/mol@LB@Hybridization Energy -45.9009 kcal/mol@LB@Unfolding Energy - mRNA 14.0255 kcal/mol@LB@Unfolding Energy - sRNA 22.2914 kcal/mol@LB@@LB@Position - mRNA 211 -- 267@LB@Position - sRNA 155 -- 213@LB@Position Seed - mRNA 257 -- 263@LB@Position Seed - sRNA 158 -- 164@LB@
73	0.0193	0.6733	lmo0405	N/A	-18.50	0.020574	154 -- 209	155 -- 199	phosphate transporter	_____________153____________210\n_____________\|______________\|\n5'-GUG...UUAUG________U_____UACGA...CGA-3'\n______________AUGACGGG_AUGAG\n______________\|\|:\|\|\|\|\|_\|\|\|\|\|\n______________UAUUGCCC_UACUC\n3'-GUU...AUACG______________CGUAU...GGA-5'\n_____________\|______________\|\n_____________200____________154\n	50 -- 56	202 -- 208	-16.311 kcal/mol	2.72131 kcal/mol	0.386761 kcal/mol	153 210@LB@ \| \|@LB@5'-GUG...UUAUG U UACGA...CGA-3'@LB@ AUGACGGG AUGAG@LB@ \|\|:\|\|\|\|\| \|\|\|\|\|@LB@ UAUUGCCC UACUC@LB@3'-GUU...AUACG CGUAU...GGA-5'@LB@ \| \|@LB@ 200 154@LB@@LB@@LB@Energy -18.50 kcal/mol@LB@Hybridization Energy -16.311 kcal/mol@LB@Unfolding Energy - mRNA 2.72131 kcal/mol@LB@Unfolding Energy - sRNA 0.386761 kcal/mol@LB@@LB@Position - mRNA 154 -- 209@LB@Position - sRNA 155 -- 199@LB@Position Seed - mRNA 50 -- 56@LB@Position Seed - sRNA 202 -- 208@LB@
115	0.03087	0.6968	lmo0059	N/A	-17.38	0.034592	142 -- 189	155 -- 211		_____________141_______________________________________________190\n_____________\|_________________________________________________\|\n5'-AAG...UUAGC____G_____UU___U________________UUUU_____A_______GGGAG...CAU-3'\n______________AGGC__GGAU__AUG_GGCUGCGCG__CAAUG____UGAAU_GAAAGCG\n______________\|\|\|\|__:\|\|:__\|\|\|_::\|\|:\|:\|\|__\|\|\|\|\|____\|\|\|\|\|_\|\|\|\|:\|:\n______________UCCG__UCUG__UAC_UUGAUGUGC__GUUAC____ACUUA_CUUUUGU\n3'-GUU...CCUAC____UA___________________CG_____UCU______________UUAUU...GGA-5'\n_____________\|_________________________________________________\|\n_____________212_______________________________________________154\n	183 -- 189	155 -- 161	-35.3318 kcal/mol	12.9952 kcal/mol	13.976 kcal/mol	141 190@LB@ \| \|@LB@5'-AAG...UUAGC G UU U UUUU A GGGAG...CAU-3'@LB@ AGGC GGAU AUG GGCUGCGCG CAAUG UGAAU GAAAGCG@LB@ \|\|\|\| :\|\|: \|\|\| ::\|\|:\|:\|\| \|\|\|\|\| \|\|\|\|\| \|\|\|\|:\|:@LB@ UCCG UCUG UAC UUGAUGUGC GUUAC ACUUA CUUUUGU@LB@3'-GUU...CCUAC UA CG UCU UUAUU...GGA-5'@LB@ \| \|@LB@ 212 154@LB@@LB@@LB@Energy -17.38 kcal/mol@LB@Hybridization Energy -35.3318 kcal/mol@LB@Unfolding Energy - mRNA 12.9952 kcal/mol@LB@Unfolding Energy - sRNA 13.976 kcal/mol@LB@@LB@Position - mRNA 142 -- 189@LB@Position - sRNA 155 -- 211@LB@Position Seed - mRNA 183 -- 189@LB@Position Seed - sRNA 155 -- 161@LB@
122	0.03252	0.6968	lmo0926	N/A	-16.71	0.046694	198 -- 274	155 -- 207	TetR family transcriptional regulator	_____________197______________275\n_____________\|________________\|\n5'-GCU...AACCA_____________G__GCGCC...UCG-3'\n______________AGAUGAGUGAAAA_CA\n______________\|\|\|\|\|\|:\|\|\|\|\|\|_\|\|\n______________UCUACUUACUUUU_GU\n3'-GUU...GUUAC________________UUAUU...GGA-5'\n_____________\|________________\|\n_____________208______________154\n	265 -- 271	157 -- 163	-16.1904 kcal/mol	1.95479 kcal/mol	0.920152 kcal/mol	197 275@LB@ \| \|@LB@5'-GCU...AACCA G GCGCC...UCG-3'@LB@ AGAUGAGUGAAAA CA@LB@ \|\|\|\|\|\|:\|\|\|\|\|\| \|\|@LB@ UCUACUUACUUUU GU@LB@3'-GUU...GUUAC UUAUU...GGA-5'@LB@ \| \|@LB@ 208 154@LB@@LB@@LB@Energy -16.71 kcal/mol@LB@Hybridization Energy -16.1904 kcal/mol@LB@Unfolding Energy - mRNA 1.95479 kcal/mol@LB@Unfolding Energy - sRNA 0.920152 kcal/mol@LB@@LB@Position - mRNA 198 -- 274@LB@Position - sRNA 155 -- 207@LB@Position Seed - mRNA 265 -- 271@LB@Position Seed - sRNA 157 -- 163@LB@
152	0.04001	0.7027	lmo1203	cbiL	-16.49	0.051377	174 -- 199	155 -- 181	cobalt-precorrin-2 C(20)-methyltransferase	_____________173___________________200\n_____________\|_____________________\|\n5'-ACA...CACUG____A________________CGUGG...CAC-3'\n______________CAAU_GAGA_GGGUGAAAACA\n______________\|\|\|\|_\|\|\|\|_\|::\|\|\|\|\|\|\|\|\n______________GUUA_CUCU_CUUACUUUUGU\n3'-GUU...UGCCG_________A___________UUAUU...GGA-5'\n_____________\|_____________________\|\n_____________182___________________154\n	193 -- 199	155 -- 161	-18.5314 kcal/mol	4.87195 kcal/mol	0.95817 kcal/mol	173 200@LB@ \| \|@LB@5'-ACA...CACUG A CGUGG...CAC-3'@LB@ CAAU GAGA GGGUGAAAACA@LB@ \|\|\|\| \|\|\|\| \|::\|\|\|\|\|\|\|\|@LB@ GUUA CUCU CUUACUUUUGU@LB@3'-GUU...UGCCG A UUAUU...GGA-5'@LB@ \| \|@LB@ 182 154@LB@@LB@@LB@Energy -16.49 kcal/mol@LB@Hybridization Energy -18.5314 kcal/mol@LB@Unfolding Energy - mRNA 4.87195 kcal/mol@LB@Unfolding Energy - sRNA 0.95817 kcal/mol@LB@@LB@Position - mRNA 174 -- 199@LB@Position - sRNA 155 -- 181@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 155 -- 161@LB@
155	0.04112	0.7058	lmo1165	N/A	-16.89	0.043077	88 -- 150	155 -- 206	ethanolamine utilization protein EutE	_____________87_______________________________151\n_____________\|________________________________\|\n5'-AAG...AAUGG____________G___C_______G_______UCCAC...CAA-3'\n______________GGAUUA_GCGGC_GAU_AG_UGGA_GAAGAUA\n______________\|:\|:\|\|_:\|\|\|\|_:\|\|_\|\|_\|\|:\|_\|\|\|:\|:\|\n______________CUUGAU_UGCCG_UUA_UC_ACUU_CUUUUGU\n3'-GUU...CUGUA______G_____G___C__U____A_______UUAUU...GGA-5'\n_____________\|________________________________\|\n_____________207______________________________154\n	144 -- 150	155 -- 161	-24.1777 kcal/mol	5.41964 kcal/mol	9.66922 kcal/mol	87 151@LB@ \| \|@LB@5'-AAG...AAUGG G C G UCCAC...CAA-3'@LB@ GGAUUA GCGGC GAU AG UGGA GAAGAUA@LB@ \|:\|:\|\| :\|\|\|\| :\|\| \|\| \|\|:\| \|\|\|:\|:\|@LB@ CUUGAU UGCCG UUA UC ACUU CUUUUGU@LB@3'-GUU...CUGUA G G C U A UUAUU...GGA-5'@LB@ \| \|@LB@ 207 154@LB@@LB@@LB@Energy -16.89 kcal/mol@LB@Hybridization Energy -24.1777 kcal/mol@LB@Unfolding Energy - mRNA 5.41964 kcal/mol@LB@Unfolding Energy - sRNA 9.66922 kcal/mol@LB@@LB@Position - mRNA 88 -- 150@LB@Position - sRNA 155 -- 206@LB@Position Seed - mRNA 144 -- 150@LB@Position Seed - sRNA 155 -- 161@LB@
167	0.04638	0.7403	lmo1401	N/A	-16.51	0.050998	149 -- 209	155 -- 204		_____________148________210\n_____________\|__________\|\n5'-AUU...AACAA__________CUAUU...UCA-3'\n______________AAUGAAAAUA\n______________\|\|\|\|\|\|\|\|:\|\n______________UUACUUUUGU\n3'-GUU...UCUAC__________UUAUU...GGA-5'\n_____________\|__________\|\n_____________205________154\n	203 -- 209	155 -- 161	-7.61408 kcal/mol	0.775188 kcal/mol	0.60613 kcal/mol	148 210@LB@ \| \|@LB@5'-AUU...AACAA CUAUU...UCA-3'@LB@ AAUGAAAAUA@LB@ \|\|\|\|\|\|\|\|:\|@LB@ UUACUUUUGU@LB@3'-GUU...UCUAC UUAUU...GGA-5'@LB@ \| \|@LB@ 205 154@LB@@LB@@LB@Energy -16.51 kcal/mol@LB@Hybridization Energy -7.61408 kcal/mol@LB@Unfolding Energy - mRNA 0.775188 kcal/mol@LB@Unfolding Energy - sRNA 0.60613 kcal/mol@LB@@LB@Position - mRNA 149 -- 209@LB@Position - sRNA 155 -- 204@LB@Position Seed - mRNA 203 -- 209@LB@Position Seed - sRNA 155 -- 161@LB@
175	0.04975	0.7607	lmo0194	N/A	-16.29	0.056201	181 -- 242	155 -- 212	ABC transporter ATP-binding protein	_____________180________________________243\n_____________\|__________________________\|\n_______5'-AAGA________AAACGAAAAA________AAUUU...AGC-3'\n______________CAAUGGGA__________AGUGAAGA\n______________\|\|\|\|\|:\|\|__________\|:\|\|\|\|:\|\n______________GUUACUCU__________UUACUUUU\n3'-GUU...UGCCG________AC________________GUUUA...GGA-5'\n_____________\|__________________________\|\n_____________213________________________154\n	24 -- 30	157 -- 163	-10.5893 kcal/mol	0.67181 kcal/mol	0.933292 kcal/mol	180 243@LB@ \| \|@LB@ 5'-AAGA AAACGAAAAA AAUUU...AGC-3'@LB@ CAAUGGGA AGUGAAGA@LB@ \|\|\|\|\|:\|\| \|:\|\|\|\|:\|@LB@ GUUACUCU UUACUUUU@LB@3'-GUU...UGCCG AC GUUUA...GGA-5'@LB@ \| \|@LB@ 213 154@LB@@LB@@LB@Energy -16.29 kcal/mol@LB@Hybridization Energy -10.5893 kcal/mol@LB@Unfolding Energy - mRNA 0.67181 kcal/mol@LB@Unfolding Energy - sRNA 0.933292 kcal/mol@LB@@LB@Position - mRNA 181 -- 242@LB@Position - sRNA 155 -- 212@LB@Position Seed - mRNA 24 -- 30@LB@Position Seed - sRNA 157 -- 163@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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usability assessment

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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