Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
91	0.002527	0.0977	b4393	trpR	-12.48	0.007130	9 -- 21	43 -- 55	transcriptional repressor tryptophan-binding	_____________8_____________22\n_____________\|_____________\|\n___5'-GAGACGCG_____________AACGU...AUC-3'\n______________CGGUUAUGUGAAG\n______________\|\|:\|\|\|:\|\|\|\|\|\|\n______________GCUAAUGCACUUC\n3'-AUU...UUUAG_____________AUUUU...AUG-5'\n_____________\|_____________\|\n_____________56____________42\n	15 -- 21	43 -- 49	-16.824 kcal/mol	1.08541 kcal/mol	3.2693 kcal/mol	8 22@LB@ \| \|@LB@ 5'-GAGACGCG AACGU...AUC-3'@LB@ CGGUUAUGUGAAG@LB@ \|\|:\|\|\|:\|\|\|\|\|\|@LB@ GCUAAUGCACUUC@LB@3'-AUU...UUUAG AUUUU...AUG-5'@LB@ \| \|@LB@ 56 42@LB@@LB@@LB@Energy -12.48 kcal/mol@LB@Hybridization Energy -16.824 kcal/mol@LB@Unfolding Energy - mRNA 1.08541 kcal/mol@LB@Unfolding Energy - sRNA 3.2693 kcal/mol@LB@@LB@Position - mRNA 9 -- 21@LB@Position - sRNA 43 -- 55@LB@Position Seed - mRNA 15 -- 21@LB@Position Seed - sRNA 43 -- 49@LB@
92	0.002567	0.09816	b4120	melB	-8.04	0.151444	195 -- 202	1 -- 8	melibiose:sodium symporter	_____________194______203\n_____________\|________\|\n5'-AUA...CCCGA________GAGCA...ACA-3'\n______________UACCCUAU\n______________\|\|\|\|\|\|\|:\n______________AUGGGAUG\n3'-AUU...GAGAC________-5'\n_____________\|_______\|\n_____________9_______1\n	196 -- 202	1 -- 7	-10.9179 kcal/mol	0.779946 kcal/mol	2.11603 kcal/mol	194 203@LB@ \| \|@LB@5'-AUA...CCCGA GAGCA...ACA-3'@LB@ UACCCUAU@LB@ \|\|\|\|\|\|\|:@LB@ AUGGGAUG@LB@3'-AUU...GAGAC -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -8.04 kcal/mol@LB@Hybridization Energy -10.9179 kcal/mol@LB@Unfolding Energy - mRNA 0.779946 kcal/mol@LB@Unfolding Energy - sRNA 2.11603 kcal/mol@LB@@LB@Position - mRNA 195 -- 202@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 196 -- 202@LB@Position Seed - sRNA 1 -- 7@LB@
93	0.002634	0.09947	b2202	napC	-9.04	0.084220	177 -- 197	34 -- 54	quinol dehydrogenase electron source for NapAB	_____________176___________________198\n_____________\|_____________________\|\n5'-ACG...CAAAA_______G___A_________AUUAU...GUU-3'\n______________GGUUACG_GAA_UAAGAGGUU\n______________\|:\|\|\|\|\|_\|\|\|_\|\|\|:\|\|\|\|:\n______________CUAAUGC_CUU_AUUUUCCAG\n3'-AUU...UUAGG_______A___C_________ACUUU...AUG-5'\n_____________\|_____________________\|\n_____________55____________________33\n	177 -- 183	48 -- 54	-21.2793 kcal/mol	4.43682 kcal/mol	7.71857 kcal/mol	176 198@LB@ \| \|@LB@5'-ACG...CAAAA G A AUUAU...GUU-3'@LB@ GGUUACG GAA UAAGAGGUU@LB@ \|:\|\|\|\|\| \|\|\| \|\|\|:\|\|\|\|:@LB@ CUAAUGC CUU AUUUUCCAG@LB@3'-AUU...UUAGG A C ACUUU...AUG-5'@LB@ \| \|@LB@ 55 33@LB@@LB@@LB@Energy -9.04 kcal/mol@LB@Hybridization Energy -21.2793 kcal/mol@LB@Unfolding Energy - mRNA 4.43682 kcal/mol@LB@Unfolding Energy - sRNA 7.71857 kcal/mol@LB@@LB@Position - mRNA 177 -- 197@LB@Position - sRNA 34 -- 54@LB@Position Seed - mRNA 177 -- 183@LB@Position Seed - sRNA 48 -- 54@LB@
94	0.002669	0.09947	b2659	csiD	-13.62	0.002680	28 -- 48	1 -- 21	carbon starvation protein	_____________27____________________49\n_____________\|_____________________\|\n5'-GUU...UGUCU__C________AA________GUUAA...UGG-3'\n______________CA_UUUGCCUU__UACCCUAC\n______________\|\|_:\|\|:\|\|\|:__\|\|\|\|\|\|\|\|\n______________GU_GAAUGGAG__AUGGGAUG\n3'-AUU...AUCUU__A________AC________-5'\n_____________\|____________________\|\n_____________22___________________1\n	42 -- 48	1 -- 7	-22.7197 kcal/mol	1.13457 kcal/mol	7.99195 kcal/mol	27 49@LB@ \| \|@LB@5'-GUU...UGUCU C AA GUUAA...UGG-3'@LB@ CA UUUGCCUU UACCCUAC@LB@ \|\| :\|\|:\|\|\|: \|\|\|\|\|\|\|\|@LB@ GU GAAUGGAG AUGGGAUG@LB@3'-AUU...AUCUU A AC -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -13.62 kcal/mol@LB@Hybridization Energy -22.7197 kcal/mol@LB@Unfolding Energy - mRNA 1.13457 kcal/mol@LB@Unfolding Energy - sRNA 7.99195 kcal/mol@LB@@LB@Position - mRNA 28 -- 48@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 42 -- 48@LB@Position Seed - sRNA 1 -- 7@LB@
95	0.002686	0.09947	b2419	yfeK	-12.78	0.005552	57 -- 65	1 -- 9	uncharacterized protein	_____________56________66\n_____________\|_________\|\n5'-GUU...CUGCA_________UCUUA...UGG-3'\n______________GUGCCCUAC\n______________\|\|:\|\|\|\|\|\|\n______________CAUGGGAUG\n3'-AUU...GGAGA_________-5'\n_____________\|________\|\n_____________10_______1\n	59 -- 65	1 -- 7	-16.152 kcal/mol	1.26264 kcal/mol	2.12872 kcal/mol	56 66@LB@ \| \|@LB@5'-GUU...CUGCA UCUUA...UGG-3'@LB@ GUGCCCUAC@LB@ \|\|:\|\|\|\|\|\|@LB@ CAUGGGAUG@LB@3'-AUU...GGAGA -5'@LB@ \| \|@LB@ 10 1@LB@@LB@@LB@Energy -12.78 kcal/mol@LB@Hybridization Energy -16.152 kcal/mol@LB@Unfolding Energy - mRNA 1.26264 kcal/mol@LB@Unfolding Energy - sRNA 2.12872 kcal/mol@LB@@LB@Position - mRNA 57 -- 65@LB@Position - sRNA 1 -- 9@LB@Position Seed - mRNA 59 -- 65@LB@Position Seed - sRNA 1 -- 7@LB@
96	0.002764	0.1002	b1034	ycdX	-5.77	0.451964	102 -- 115	32 -- 46	alkaline phosphatase	_____________101_____________116\n_____________\|_______________\|\n5'-GGC...AAAAA_______________CGGGC...AAC-3'\n______________GGGG_AGAGGGUCUG\n______________\|::\|_\|:\|:\|\|\|\|\|\|\n______________CUUC_UUUUCCAGAC\n3'-AUU...AUGCA____A__________UUUCU...AUG-5'\n_____________\|_______________\|\n_____________47______________31\n	109 -- 115	32 -- 38	-16.8058 kcal/mol	3.54072 kcal/mol	7.24668 kcal/mol	101 116@LB@ \| \|@LB@5'-GGC...AAAAA CGGGC...AAC-3'@LB@ GGGG AGAGGGUCUG@LB@ \|::\| \|:\|:\|\|\|\|\|\|@LB@ CUUC UUUUCCAGAC@LB@3'-AUU...AUGCA A UUUCU...AUG-5'@LB@ \| \|@LB@ 47 31@LB@@LB@@LB@Energy -5.77 kcal/mol@LB@Hybridization Energy -16.8058 kcal/mol@LB@Unfolding Energy - mRNA 3.54072 kcal/mol@LB@Unfolding Energy - sRNA 7.24668 kcal/mol@LB@@LB@Position - mRNA 102 -- 115@LB@Position - sRNA 32 -- 46@LB@Position Seed - mRNA 109 -- 115@LB@Position Seed - sRNA 32 -- 38@LB@
97	0.002764	0.1002	b3935	priA	-7.71	0.181779	237 -- 247	3 -- 14	Primosome factor n' (replication factor Y)	_____________236__________248\n_____________\|____________\|\n5'-GAA...CGCUU____________UGACU...GCG-3'\n______________CCUC_GUACCUU\n______________\|\|\|\|_\|\|\|\|\|:\|\n______________GGAG_CAUGGGA\n3'-AUU...AGAAU____A_______UG-5'\n_____________\|____________\|\n_____________15___________2\n	241 -- 247	3 -- 9	-14.2439 kcal/mol	1.19768 kcal/mol	5.35591 kcal/mol	236 248@LB@ \| \|@LB@5'-GAA...CGCUU UGACU...GCG-3'@LB@ CCUC GUACCUU@LB@ \|\|\|\| \|\|\|\|\|:\|@LB@ GGAG CAUGGGA@LB@3'-AUU...AGAAU A UG-5'@LB@ \| \|@LB@ 15 2@LB@@LB@@LB@Energy -7.71 kcal/mol@LB@Hybridization Energy -14.2439 kcal/mol@LB@Unfolding Energy - mRNA 1.19768 kcal/mol@LB@Unfolding Energy - sRNA 5.35591 kcal/mol@LB@@LB@Position - mRNA 237 -- 247@LB@Position - sRNA 3 -- 14@LB@Position Seed - mRNA 241 -- 247@LB@Position Seed - sRNA 3 -- 9@LB@
98	0.002849	0.1023	b0772	ybhC	-6.17	0.383539	27 -- 33	49 -- 55	acyl-CoA thioesterase lipoprotein	_____________26______34\n_____________\|_______\|\n5'-UCA...CGCCU_______UGCGA...AUC-3'\n______________CGAUUAC\n______________\|\|\|\|\|\|\|\n______________GCUAAUG\n3'-AUU...UUUAG_______CACUU...AUG-5'\n_____________\|_______\|\n_____________56______48\n	27 -- 33	49 -- 55	-7.86935 kcal/mol	1.39337 kcal/mol	0.311712 kcal/mol	26 34@LB@ \| \|@LB@5'-UCA...CGCCU UGCGA...AUC-3'@LB@ CGAUUAC@LB@ \|\|\|\|\|\|\|@LB@ GCUAAUG@LB@3'-AUU...UUUAG CACUU...AUG-5'@LB@ \| \|@LB@ 56 48@LB@@LB@@LB@Energy -6.17 kcal/mol@LB@Hybridization Energy -7.86935 kcal/mol@LB@Unfolding Energy - mRNA 1.39337 kcal/mol@LB@Unfolding Energy - sRNA 0.311712 kcal/mol@LB@@LB@Position - mRNA 27 -- 33@LB@Position - sRNA 49 -- 55@LB@Position Seed - mRNA 27 -- 33@LB@Position Seed - sRNA 49 -- 55@LB@
99	0.00311	0.1096	b2801	fucP	-13.12	0.004165	162 -- 172	46 -- 56	L-fucose transporter	_____________161_________173\n_____________\|___________\|\n5'-AUG...CAUAA___________CUUAA...CAC-3'\n______________CCGAUUACGUG\n______________\|\|\|\|\|\|\|\|\|\|\|\n______________GGCUAAUGCAC\n3'-AUU...GUUUA___________UUCAU...AUG-5'\n_____________\|___________\|\n_____________57__________45\n	166 -- 172	46 -- 52	-17.4041 kcal/mol	2.44124 kcal/mol	1.86194 kcal/mol	161 173@LB@ \| \|@LB@5'-AUG...CAUAA CUUAA...CAC-3'@LB@ CCGAUUACGUG@LB@ \|\|\|\|\|\|\|\|\|\|\|@LB@ GGCUAAUGCAC@LB@3'-AUU...GUUUA UUCAU...AUG-5'@LB@ \| \|@LB@ 57 45@LB@@LB@@LB@Energy -13.12 kcal/mol@LB@Hybridization Energy -17.4041 kcal/mol@LB@Unfolding Energy - mRNA 2.44124 kcal/mol@LB@Unfolding Energy - sRNA 1.86194 kcal/mol@LB@@LB@Position - mRNA 162 -- 172@LB@Position - sRNA 46 -- 56@LB@Position Seed - mRNA 166 -- 172@LB@Position Seed - sRNA 46 -- 52@LB@
100	0.003115	0.1096	b0353	mhpT	-8.28	0.132596	137 -- 146	48 -- 57	3-hydroxyphenylpropionic transporter	_____________136________147\n_____________\|__________\|\n5'-CGG...AUAGU__________CGGCU...CGG-3'\n______________UCUGAUUACG\n______________\|\|:\|\|\|\|\|\|\|\n______________AGGCUAAUGC\n3'-AUU...GGUUU__________ACUUC...AUG-5'\n_____________\|__________\|\n_____________58_________47\n	140 -- 146	48 -- 54	-13.4725 kcal/mol	3.61374 kcal/mol	1.55976 kcal/mol	136 147@LB@ \| \|@LB@5'-CGG...AUAGU CGGCU...CGG-3'@LB@ UCUGAUUACG@LB@ \|\|:\|\|\|\|\|\|\|@LB@ AGGCUAAUGC@LB@3'-AUU...GGUUU ACUUC...AUG-5'@LB@ \| \|@LB@ 58 47@LB@@LB@@LB@Energy -8.28 kcal/mol@LB@Hybridization Energy -13.4725 kcal/mol@LB@Unfolding Energy - mRNA 3.61374 kcal/mol@LB@Unfolding Energy - sRNA 1.55976 kcal/mol@LB@@LB@Position - mRNA 137 -- 146@LB@Position - sRNA 48 -- 57@LB@Position Seed - mRNA 140 -- 146@LB@Position Seed - sRNA 48 -- 54@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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