Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
171	0.00718	0.1466	b1708	nlpC	-9.44	0.065343	288 -- 298	49 -- 59	putative C40 clan peptidase lipoprotein		_____________287_________299\n_____________\|___________\|\n5'-AAA...UUUCU___________CG-3'\n______________GAUUCGAUUAC\n______________:\|\|:\|\|\|\|\|\|\|\n______________UUAGGCUAAUG\n3'-AUU...UCGGU___________CACUU...AUG-5'\n_____________\|___________\|\n_____________60__________48\n	292 -- 298	49 -- 55	-11.5278 kcal/mol	1.77565 kcal/mol	0.318405 kcal/mol	287 299@LB@ \| \|@LB@5'-AAA...UUUCU CG-3'@LB@ GAUUCGAUUAC@LB@ :\|\|:\|\|\|\|\|\|\|@LB@ UUAGGCUAAUG@LB@3'-AUU...UCGGU CACUU...AUG-5'@LB@ \| \|@LB@ 60 48@LB@@LB@@LB@Energy -9.44 kcal/mol@LB@Hybridization Energy -11.5278 kcal/mol@LB@Unfolding Energy - mRNA 1.77565 kcal/mol@LB@Unfolding Energy - sRNA 0.318405 kcal/mol@LB@@LB@Position - mRNA 288 -- 298@LB@Position - sRNA 49 -- 59@LB@Position Seed - mRNA 292 -- 298@LB@Position Seed - sRNA 49 -- 55@LB@
172	0.007198	0.1466	b2395	yfeA	-7.88	0.166321	272 -- 292	49 -- 69	putative diguanylate cyclase		_____________271____________________293\n_____________\|______________________\|\n5'-ACC...GUACU__________UC____UU____UUCGCCAC-3'\n______________UAUUCAGCUA___CCG__UUAU\n______________\|\|\|\|\|\|\|\|:\|___\|\|\|__\|\|\|:\n______________AUAAGUCGGU___GGC__AAUG\n3'-AUU...AUUUU__________UUA___U_____CACUU...AUG-5'\n_____________\|______________________\|\n_____________70_____________________48\n	275 -- 281	60 -- 66	-16.2546 kcal/mol	2.18624 kcal/mol	6.21499 kcal/mol	271 293@LB@ \| \|@LB@5'-ACC...GUACU UC UU UUCGCCAC-3'@LB@ UAUUCAGCUA CCG UUAU@LB@ \|\|\|\|\|\|\|\|:\| \|\|\| \|\|\|:@LB@ AUAAGUCGGU GGC AAUG@LB@3'-AUU...AUUUU UUA U CACUU...AUG-5'@LB@ \| \|@LB@ 70 48@LB@@LB@@LB@Energy -7.88 kcal/mol@LB@Hybridization Energy -16.2546 kcal/mol@LB@Unfolding Energy - mRNA 2.18624 kcal/mol@LB@Unfolding Energy - sRNA 6.21499 kcal/mol@LB@@LB@Position - mRNA 272 -- 292@LB@Position - sRNA 49 -- 69@LB@Position Seed - mRNA 275 -- 281@LB@Position Seed - sRNA 60 -- 66@LB@
173	0.007216	0.1466	b3650	spoT	-5.39	0.520602	11 -- 17	54 -- 60	bifunctional (p)ppGpp synthetase II/ guanosine-3' 5'-bis pyrophosphate 3'-pyrophosphohydrolase		_____________10______18\n_____________\|_______\|\n5'-UAG...GGAAA_______CUGGU...CUC-3'\n______________GGAUCCG\n______________::\|\|\|\|\|\n______________UUUAGGC\n3'-AUU...GUCGG_______UAAUG...AUG-5'\n_____________\|_______\|\n_____________61______53\n	11 -- 17	54 -- 60	-6.87427 kcal/mol	1.21245 kcal/mol	0.286889 kcal/mol	10 18@LB@ \| \|@LB@5'-UAG...GGAAA CUGGU...CUC-3'@LB@ GGAUCCG@LB@ ::\|\|\|\|\|@LB@ UUUAGGC@LB@3'-AUU...GUCGG UAAUG...AUG-5'@LB@ \| \|@LB@ 61 53@LB@@LB@@LB@Energy -5.39 kcal/mol@LB@Hybridization Energy -6.87427 kcal/mol@LB@Unfolding Energy - mRNA 1.21245 kcal/mol@LB@Unfolding Energy - sRNA 0.286889 kcal/mol@LB@@LB@Position - mRNA 11 -- 17@LB@Position - sRNA 54 -- 60@LB@Position Seed - mRNA 11 -- 17@LB@Position Seed - sRNA 54 -- 60@LB@
174	0.007262	0.1466	b0473	htpG	-8.02	0.153160	10 -- 17	50 -- 57	protein refolding molecular co-chaperone Hsp90 Hsp70-dependent; heat-shock protein; ATPase		_____________9________18\n_____________\|________\|\n5'-UCA...GGCGU________GCGGC...GUG-3'\n______________UCCGGUUG\n______________\|\|\|\|:\|\|:\n______________AGGCUAAU\n3'-AUU...GGUUU________GCACU...AUG-5'\n_____________\|________\|\n_____________58_______49\n	11 -- 17	50 -- 56	-9.07782 kcal/mol	0.753256 kcal/mol	0.317388 kcal/mol	9 18@LB@ \| \|@LB@5'-UCA...GGCGU GCGGC...GUG-3'@LB@ UCCGGUUG@LB@ \|\|\|\|:\|\|:@LB@ AGGCUAAU@LB@3'-AUU...GGUUU GCACU...AUG-5'@LB@ \| \|@LB@ 58 49@LB@@LB@@LB@Energy -8.02 kcal/mol@LB@Hybridization Energy -9.07782 kcal/mol@LB@Unfolding Energy - mRNA 0.753256 kcal/mol@LB@Unfolding Energy - sRNA 0.317388 kcal/mol@LB@@LB@Position - mRNA 10 -- 17@LB@Position - sRNA 50 -- 57@LB@Position Seed - mRNA 11 -- 17@LB@Position Seed - sRNA 50 -- 56@LB@
175	0.007291	0.1466	b1498	ydeN	-8.19	0.139530	95 -- 124	45 -- 67	putative Ser-type periplasmic non-aryl sulfatase	b3678 b3801	_____________94_____________________________125\n_____________\|______________________________\|\n5'-AUU...CUUUU______________CUAUCUCU________CUAAC...UAA-3'\n______________UUUAGCUAAAUCUG________UUAUGUGA\n______________\|\|:\|\|\|:\|\|\|\|\|:\|________\|\|\|:\|\|\|\|\n______________AAGUCGGUUUAGGC________AAUGCACU\n3'-AUU...UUUAU______________U_______________UCAUU...AUG-5'\n_____________\|______________________________\|\n_____________68_____________________________44\n	118 -- 124	45 -- 51	-22.2428 kcal/mol	5.90754 kcal/mol	8.16749 kcal/mol	94 125@LB@ \| \|@LB@5'-AUU...CUUUU CUAUCUCU CUAAC...UAA-3'@LB@ UUUAGCUAAAUCUG UUAUGUGA@LB@ \|\|:\|\|\|:\|\|\|\|\|:\| \|\|\|:\|\|\|\|@LB@ AAGUCGGUUUAGGC AAUGCACU@LB@3'-AUU...UUUAU U UCAUU...AUG-5'@LB@ \| \|@LB@ 68 44@LB@@LB@@LB@Energy -8.19 kcal/mol@LB@Hybridization Energy -22.2428 kcal/mol@LB@Unfolding Energy - mRNA 5.90754 kcal/mol@LB@Unfolding Energy - sRNA 8.16749 kcal/mol@LB@@LB@Position - mRNA 95 -- 124@LB@Position - sRNA 45 -- 67@LB@Position Seed - mRNA 118 -- 124@LB@Position Seed - sRNA 45 -- 51@LB@
176	0.007357	0.147	b2905	gcvT	-7.40	0.214118	198 -- 224	6 -- 30	aminomethyltransferase tetrahydrofolate-dependent subunit (T protein) of glycine cleavage complex		_____________197__________________________225\n_____________\|____________________________\|\n5'-CUG...AGGAC_______CAC____GACU__________GAACA...AAA-3'\n______________AAGAUGG___AACA_____CCUUUGUAC\n______________\|\|\|\|\|:\|___\|\|\|\|_____\|\|\|:\|\|\|\|\|\n______________UUCUAUC___UUGU_____GGAGACAUG\n3'-AUU...AGACU______________AGAAU_________GGAUG-5'\n_____________\|____________________________\|\n_____________31___________________________5\n	218 -- 224	6 -- 12	-21.3805 kcal/mol	5.97871 kcal/mol	8.02863 kcal/mol	197 225@LB@ \| \|@LB@5'-CUG...AGGAC CAC GACU GAACA...AAA-3'@LB@ AAGAUGG AACA CCUUUGUAC@LB@ \|\|\|\|\|:\| \|\|\|\| \|\|\|:\|\|\|\|\|@LB@ UUCUAUC UUGU GGAGACAUG@LB@3'-AUU...AGACU AGAAU GGAUG-5'@LB@ \| \|@LB@ 31 5@LB@@LB@@LB@Energy -7.40 kcal/mol@LB@Hybridization Energy -21.3805 kcal/mol@LB@Unfolding Energy - mRNA 5.97871 kcal/mol@LB@Unfolding Energy - sRNA 8.02863 kcal/mol@LB@@LB@Position - mRNA 198 -- 224@LB@Position - sRNA 6 -- 30@LB@Position Seed - mRNA 218 -- 224@LB@Position Seed - sRNA 6 -- 12@LB@
177	0.007672	0.1511	b2307	hisM	-10.48	0.032509	175 -- 237	1 -- 57	histidine ABC transporter permease		_____________174__________________________________________________________________238\n_____________\|____________________________________________________________________\|\n5'-CUC...GCUAC____UGGG___________________CCUGU___C___________AUGAAUA___GAA___G____UGUGG...CGG-3'\n______________UCCG____UGUGAAG___AGGG_CUGA_____GAU_GAA_AUCUUAC_______CUG___ACC_CUGC\n______________\|\|\|\|____:\|\|\|\|\|\|___\|:\|\|_\|\|\|\|_____\|\|\|_\|\|\|_\|\|\|\|\|\|\|_______\|\|\|___\|\|\|_\|\|:\|\n______________AGGC____GCACUUC___UUCC_GACU_____CUA_CUU_UAGAAUG_______GAC___UGG_GAUG\n3'-AUU...GGUUU____UAAU_______AUU____A____UU______U___G_______GA________A__________-5'\n_____________\|___________________________________________________________________\|\n_____________58__________________________________________________________________1\n	210 -- 216	14 -- 20	-38.5224 kcal/mol	15.358 kcal/mol	12.7026 kcal/mol	174 238@LB@ \| \|@LB@5'-CUC...GCUAC UGGG CCUGU C AUGAAUA GAA G UGUGG...CGG-3'@LB@ UCCG UGUGAAG AGGG CUGA GAU GAA AUCUUAC CUG ACC CUGC@LB@ \|\|\|\| :\|\|\|\|\|\| \|:\|\| \|\|\|\| \|\|\| \|\|\| \|\|\|\|\|\|\| \|\|\| \|\|\| \|\|:\|@LB@ AGGC GCACUUC UUCC GACU CUA CUU UAGAAUG GAC UGG GAUG@LB@3'-AUU...GGUUU UAAU AUU A UU U G GA A -5'@LB@ \| \|@LB@ 58 1@LB@@LB@@LB@Energy -10.48 kcal/mol@LB@Hybridization Energy -38.5224 kcal/mol@LB@Unfolding Energy - mRNA 15.358 kcal/mol@LB@Unfolding Energy - sRNA 12.7026 kcal/mol@LB@@LB@Position - mRNA 175 -- 237@LB@Position - sRNA 1 -- 57@LB@Position Seed - mRNA 210 -- 216@LB@Position Seed - sRNA 14 -- 20@LB@
178	0.007695	0.1511	b4567	yjjZ	-7.25	0.231371	185 -- 218	2 -- 33	uncharacterized protein		_____________184_________________________________219\n_____________\|___________________________________\|\n5'-CGG...AUCAA_______GAU___AU___G____AA______G___GGCAG...GGA-3'\n______________UGAAAGA___GAA__CAU_UUGC__C_GUAC_CUG\n______________\|\|\|\|\|\|\|___\|\|\|__\|\|\|_\|\|:\|__\|_\|\|\|\|_\|\|:\n______________ACUUUCU___CUU__GUA_AAUG__G_CAUG_GAU\n3'-AUU...UCCAG_______AU_________G____GA_A____G___G-5'\n_____________\|___________________________________\|\n_____________34__________________________________1\n	185 -- 191	27 -- 33	-22.8177 kcal/mol	6.54395 kcal/mol	9.04499 kcal/mol	184 219@LB@ \| \|@LB@5'-CGG...AUCAA GAU AU G AA G GGCAG...GGA-3'@LB@ UGAAAGA GAA CAU UUGC C GUAC CUG@LB@ \|\|\|\|\|\|\| \|\|\| \|\|\| \|\|:\| \| \|\|\|\| \|\|:@LB@ ACUUUCU CUU GUA AAUG G CAUG GAU@LB@3'-AUU...UCCAG AU G GA A G G-5'@LB@ \| \|@LB@ 34 1@LB@@LB@@LB@Energy -7.25 kcal/mol@LB@Hybridization Energy -22.8177 kcal/mol@LB@Unfolding Energy - mRNA 6.54395 kcal/mol@LB@Unfolding Energy - sRNA 9.04499 kcal/mol@LB@@LB@Position - mRNA 185 -- 218@LB@Position - sRNA 2 -- 33@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 27 -- 33@LB@
179	0.007709	0.1511	b4111	proP	-8.76	0.100034	225 -- 232	49 -- 56	proline/glycine betaine transporter		_____________224______233\n_____________\|________\|\n5'-CAC...UAAAA________CCUUC...GUA-3'\n______________CCGAUUAC\n______________\|\|\|\|\|\|\|\|\n______________GGCUAAUG\n3'-AUU...GUUUA________CACUU...AUG-5'\n_____________\|________\|\n_____________57_______48\n	226 -- 232	49 -- 55	-10.6643 kcal/mol	1.60159 kcal/mol	0.317884 kcal/mol	224 233@LB@ \| \|@LB@5'-CAC...UAAAA CCUUC...GUA-3'@LB@ CCGAUUAC@LB@ \|\|\|\|\|\|\|\|@LB@ GGCUAAUG@LB@3'-AUU...GUUUA CACUU...AUG-5'@LB@ \| \|@LB@ 57 48@LB@@LB@@LB@Energy -8.76 kcal/mol@LB@Hybridization Energy -10.6643 kcal/mol@LB@Unfolding Energy - mRNA 1.60159 kcal/mol@LB@Unfolding Energy - sRNA 0.317884 kcal/mol@LB@@LB@Position - mRNA 225 -- 232@LB@Position - sRNA 49 -- 56@LB@Position Seed - mRNA 226 -- 232@LB@Position Seed - sRNA 49 -- 55@LB@
180	0.007734	0.1511	b3617	kbl	-7.53	0.200168	193 -- 210	43 -- 60	glycine C-acetyltransferase		_____________192________________211\n_____________\|__________________\|\n5'-UUU...CUGGA_____GCAA_________AAUUU...AAG-3'\n______________GAAUC____UGCGUGGAG\n______________:\|\|\|\|____\|:\|\|\|\|:\|\|\n______________UUUAG____AUGCACUUC\n3'-AUU...GUCGG_____GCUA_________AUUUU...AUG-5'\n_____________\|__________________\|\n_____________61_________________42\n	204 -- 210	43 -- 49	-15.3085 kcal/mol	4.51554 kcal/mol	3.27548 kcal/mol	192 211@LB@ \| \|@LB@5'-UUU...CUGGA GCAA AAUUU...AAG-3'@LB@ GAAUC UGCGUGGAG@LB@ :\|\|\|\| \|:\|\|\|\|:\|\|@LB@ UUUAG AUGCACUUC@LB@3'-AUU...GUCGG GCUA AUUUU...AUG-5'@LB@ \| \|@LB@ 61 42@LB@@LB@@LB@Energy -7.53 kcal/mol@LB@Hybridization Energy -15.3085 kcal/mol@LB@Unfolding Energy - mRNA 4.51554 kcal/mol@LB@Unfolding Energy - sRNA 3.27548 kcal/mol@LB@@LB@Position - mRNA 193 -- 210@LB@Position - sRNA 43 -- 60@LB@Position Seed - mRNA 204 -- 210@LB@Position Seed - sRNA 43 -- 49@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching