Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
134	0.006141	0.1605	b3413	gntX	-10.72	0.183370	226 -- 267	37 -- 75	DNA catabolic protein	_____________225__________________________________________268\n_____________\|____________________________________________\|\n5'-CCC...AUGCU___________AAU__CACUGG____AGG_______________GUUCG...AUA-3'\n______________GGCUAUGC_CG___GC______CGUU___UCAUU_GGGGGAUUU\n______________\|:\|\|\|::\|_\|:___\|\|______\|\|\|\|___\|\|\|:\|_:\|\|\|\|\|\|\|\|\n______________CUGAUGUG_GU___CG______GCAA___AGUGA_UCCCCUAAA\n3'-UUU...CGUUU________U_______UUAA______CAA_____G_________GGUAC...GCA-5'\n_____________\|____________________________________________\|\n_____________76___________________________________________36\n	261 -- 267	37 -- 43	-30.3916 kcal/mol	14.8943 kcal/mol	4.77966 kcal/mol	225 268@LB@ \| \|@LB@5'-CCC...AUGCU AAU CACUGG AGG GUUCG...AUA-3'@LB@ GGCUAUGC CG GC CGUU UCAUU GGGGGAUUU@LB@ \|:\|\|\|::\| \|: \|\| \|\|\|\| \|\|\|:\| :\|\|\|\|\|\|\|\|@LB@ CUGAUGUG GU CG GCAA AGUGA UCCCCUAAA@LB@3'-UUU...CGUUU U UUAA CAA G GGUAC...GCA-5'@LB@ \| \|@LB@ 76 36@LB@@LB@@LB@Energy -10.72 kcal/mol@LB@Hybridization Energy -30.3916 kcal/mol@LB@Unfolding Energy - mRNA 14.8943 kcal/mol@LB@Unfolding Energy - sRNA 4.77966 kcal/mol@LB@@LB@Position - mRNA 226 -- 267@LB@Position - sRNA 37 -- 75@LB@Position Seed - mRNA 261 -- 267@LB@Position Seed - sRNA 37 -- 43@LB@
21	0.0001827	0.02926	b1937	fliE	-13.91	0.039102	192 -- 221	38 -- 77	flagellar basal-body component	_____________191________________________________________222\n_____________\|__________________________________________\|\n5'-CUG...UCCGG_____GAGA___U_______A_____________________GAAGG...GUU-3'\n______________GAGAC____AUG_CAGCGAU_____________CAGGGGAUU\n______________:\|\|\|\|____\|::_\|\|\|\|:\|\|_____________\|\|\|\|\|\|\|\|\|\n______________UUCUG____UGU_GUCGUUA_____________GUCCCCUAA\n3'-UUU...CCCGU_____AUG____________AGCAACAAAGUGA_________AGGUA...GCA-5'\n_____________\|__________________________________________\|\n_____________78_________________________________________37\n	215 -- 221	38 -- 44	-25.7557 kcal/mol	7.06678 kcal/mol	4.77969 kcal/mol	191 222@LB@ \| \|@LB@5'-CUG...UCCGG GAGA U A GAAGG...GUU-3'@LB@ GAGAC AUG CAGCGAU CAGGGGAUU@LB@ :\|\|\|\| \|:: \|\|\|\|:\|\| \|\|\|\|\|\|\|\|\|@LB@ UUCUG UGU GUCGUUA GUCCCCUAA@LB@3'-UUU...CCCGU AUG AGCAACAAAGUGA AGGUA...GCA-5'@LB@ \| \|@LB@ 78 37@LB@@LB@@LB@Energy -13.91 kcal/mol@LB@Hybridization Energy -25.7557 kcal/mol@LB@Unfolding Energy - mRNA 7.06678 kcal/mol@LB@Unfolding Energy - sRNA 4.77969 kcal/mol@LB@@LB@Position - mRNA 192 -- 221@LB@Position - sRNA 38 -- 77@LB@Position Seed - mRNA 215 -- 221@LB@Position Seed - sRNA 38 -- 44@LB@
24	0.0002076	0.02926	b3459	panM	-18.00	0.002751	9 -- 48	38 -- 75	PanD autocleavage accelerator panothenate synthesis	_____________8___________________________________________49\n_____________\|___________________________________________\|\n___5'-AAAAUAAC_______A_G___GGAAU_____ACUA________________AUGCU...UUG-3'\n______________GGC_ACA_A_GGC_____AAUUC______UCAUUCAGGGGAUU\n______________\|:\|_\|\|\|_\|_:\|\|_____\|\|\|\|\|______\|\|\|:\|\|\|\|\|\|\|\|\|\|\n______________CUG_UGU_U_UCG_____UUAAG______AGUGAGUCCCCUAA\n3'-UUU...CGUUU___A___G_G_____________CAACAA______________AGGUA...GCA-5'\n_____________\|___________________________________________\|\n_____________76__________________________________________37\n	42 -- 48	38 -- 44	-31.5918 kcal/mol	8.81239 kcal/mol	4.77946 kcal/mol	8 49@LB@ \| \|@LB@ 5'-AAAAUAAC A G GGAAU ACUA AUGCU...UUG-3'@LB@ GGC ACA A GGC AAUUC UCAUUCAGGGGAUU@LB@ \|:\| \|\|\| \| :\|\| \|\|\|\|\| \|\|\|:\|\|\|\|\|\|\|\|\|\|@LB@ CUG UGU U UCG UUAAG AGUGAGUCCCCUAA@LB@3'-UUU...CGUUU A G G CAACAA AGGUA...GCA-5'@LB@ \| \|@LB@ 76 37@LB@@LB@@LB@Energy -18.00 kcal/mol@LB@Hybridization Energy -31.5918 kcal/mol@LB@Unfolding Energy - mRNA 8.81239 kcal/mol@LB@Unfolding Energy - sRNA 4.77946 kcal/mol@LB@@LB@Position - mRNA 9 -- 48@LB@Position - sRNA 38 -- 75@LB@Position Seed - mRNA 42 -- 48@LB@Position Seed - sRNA 38 -- 44@LB@
25	0.0002079	0.02926	b4064	ghxP	-13.58	0.046769	115 -- 151	38 -- 73	guanine/hypoxanthine permease high affinity; guanine/hypoxanthine:H+ symporter	_____________114____________________________________152\n_____________\|______________________________________\|\n5'-UCG...CAAAC___GA_____G__GAUAAC________GG_________GUCCG...UAA-3'\n______________CUG___GCAG_AA______GUUUCGCU__CAGGGGAUU\n______________\|\|:___:\|\|\|_\|\|______\|\|\|\|\|:\|\|__\|\|\|\|\|\|\|\|\|\n______________GAU___UGUC_UU______CAAAGUGA__GUCCCCUAA\n3'-UUU...UUUCU___GUG____G__AAGCAA___________________AGGUA...GCA-5'\n_____________\|______________________________________\|\n_____________74_____________________________________37\n	145 -- 151	38 -- 44	-32.2199 kcal/mol	13.8672 kcal/mol	4.77333 kcal/mol	114 152@LB@ \| \|@LB@5'-UCG...CAAAC GA G GAUAAC GG GUCCG...UAA-3'@LB@ CUG GCAG AA GUUUCGCU CAGGGGAUU@LB@ \|\|: :\|\|\| \|\| \|\|\|\|\|:\|\| \|\|\|\|\|\|\|\|\|@LB@ GAU UGUC UU CAAAGUGA GUCCCCUAA@LB@3'-UUU...UUUCU GUG G AAGCAA AGGUA...GCA-5'@LB@ \| \|@LB@ 74 37@LB@@LB@@LB@Energy -13.58 kcal/mol@LB@Hybridization Energy -32.2199 kcal/mol@LB@Unfolding Energy - mRNA 13.8672 kcal/mol@LB@Unfolding Energy - sRNA 4.77333 kcal/mol@LB@@LB@Position - mRNA 115 -- 151@LB@Position - sRNA 38 -- 73@LB@Position Seed - mRNA 145 -- 151@LB@Position Seed - sRNA 38 -- 44@LB@
30	0.0004324	0.05073	b3364	tsgA	-16.38	0.008733	203 -- 236	38 -- 75	putative transporter	_____________202_____________________________________237\n_____________\|_______________________________________\|\n5'-CCA...AUCAU___________________CAUCAA______CAU_____AGCUU...AUA-3'\n______________GACUA___ACAGCAAU_CG_______GCUCA___GGAUU\n______________\|\|\|\|\|___\|\|\|\|\|\|\|\|_\|\|_______:\|\|\|\|___\|\|\|\|\|\n______________CUGAU___UGUCGUUA_GC_______UGAGU___CCUAA\n3'-UUU...CGUUU_____GUG________A__AACAAAG_____CC______AGGUA...GCA-5'\n_____________\|_______________________________________\|\n_____________76______________________________________37\n	209 -- 215	60 -- 66	-26.4683 kcal/mol	5.30565 kcal/mol	4.77946 kcal/mol	202 237@LB@ \| \|@LB@5'-CCA...AUCAU CAUCAA CAU AGCUU...AUA-3'@LB@ GACUA ACAGCAAU CG GCUCA GGAUU@LB@ \|\|\|\|\| \|\|\|\|\|\|\|\| \|\| :\|\|\|\| \|\|\|\|\|@LB@ CUGAU UGUCGUUA GC UGAGU CCUAA@LB@3'-UUU...CGUUU GUG A AACAAAG CC AGGUA...GCA-5'@LB@ \| \|@LB@ 76 37@LB@@LB@@LB@Energy -16.38 kcal/mol@LB@Hybridization Energy -26.4683 kcal/mol@LB@Unfolding Energy - mRNA 5.30565 kcal/mol@LB@Unfolding Energy - sRNA 4.77946 kcal/mol@LB@@LB@Position - mRNA 203 -- 236@LB@Position - sRNA 38 -- 75@LB@Position Seed - mRNA 209 -- 215@LB@Position Seed - sRNA 60 -- 66@LB@
59	0.002304	0.1363	b2508	guaB	-11.36	0.139274	1 -- 26	38 -- 71	IMP dehydrogenase	______________1__________________________________27\n______________\|__________________________________\|\n___________5'-__UA_______AA____________G_________GAGAA...AGC-3'\n______________AC__ACGGUAA___________AUU_CAGGGGAUU\n______________\|\|__\|\|:\|:\|\|___________\|:\|_\|\|\|\|\|\|\|\|\|\n______________UG__UGUCGUU___________UGA_GUCCCCUAA\n3'-UUU...UCUGA__UG_______AAGCAACAAAG_____________AGGUA...GCA-5'\n_____________\|___________________________________\|\n_____________72__________________________________37\n	20 -- 26	38 -- 44	-21.1303 kcal/mol	5.02575 kcal/mol	4.74986 kcal/mol	1 27@LB@ \| \|@LB@ 5'- UA AA G GAGAA...AGC-3'@LB@ AC ACGGUAA AUU CAGGGGAUU@LB@ \|\| \|\|:\|:\|\| \|:\| \|\|\|\|\|\|\|\|\|@LB@ UG UGUCGUU UGA GUCCCCUAA@LB@3'-UUU...UCUGA UG AAGCAACAAAG AGGUA...GCA-5'@LB@ \| \|@LB@ 72 37@LB@@LB@@LB@Energy -11.36 kcal/mol@LB@Hybridization Energy -21.1303 kcal/mol@LB@Unfolding Energy - mRNA 5.02575 kcal/mol@LB@Unfolding Energy - sRNA 4.74986 kcal/mol@LB@@LB@Position - mRNA 1 -- 26@LB@Position - sRNA 38 -- 71@LB@Position Seed - mRNA 20 -- 26@LB@Position Seed - sRNA 38 -- 44@LB@
96	0.004462	0.1538	b3260	dusB	-10.19	0.227205	72 -- 117	38 -- 74	tRNA-dihydrouridine synthase B	_____________71_____________________________________________118\n_____________\|______________________________________________\|\n5'-ACU...AUCGC__C____U__UUUUAAAU_______U___AUC__U_____A_____GGUCA...GAG-3'\n______________AC_ACGC_CA________GCAAUUC_UUG___CA_CUCAG_GGAUU\n______________\|\|_\|\|:\|_\|\|________\|\|\|\|\|\|\|_\|\|\|___\|\|_\|\|\|\|\|_\|\|\|\|\|\n______________UG_UGUG_GU________CGUUAAG_AAC___GU_GAGUC_CCUAA\n3'-UUU...GUUUC__A____U_________________C___AAA________C_____AGGUA...GCA-5'\n_____________\|______________________________________________\|\n_____________75_____________________________________________37\n	90 -- 96	58 -- 64	-26.7161 kcal/mol	11.7467 kcal/mol	4.77867 kcal/mol	71 118@LB@ \| \|@LB@5'-ACU...AUCGC C U UUUUAAAU U AUC U A GGUCA...GAG-3'@LB@ AC ACGC CA GCAAUUC UUG CA CUCAG GGAUU@LB@ \|\| \|\|:\| \|\| \|\|\|\|\|\|\| \|\|\| \|\| \|\|\|\|\| \|\|\|\|\|@LB@ UG UGUG GU CGUUAAG AAC GU GAGUC CCUAA@LB@3'-UUU...GUUUC A U C AAA C AGGUA...GCA-5'@LB@ \| \|@LB@ 75 37@LB@@LB@@LB@Energy -10.19 kcal/mol@LB@Hybridization Energy -26.7161 kcal/mol@LB@Unfolding Energy - mRNA 11.7467 kcal/mol@LB@Unfolding Energy - sRNA 4.77867 kcal/mol@LB@@LB@Position - mRNA 72 -- 117@LB@Position - sRNA 38 -- 74@LB@Position Seed - mRNA 90 -- 96@LB@Position Seed - sRNA 58 -- 64@LB@
112	0.004986	0.1545	b0118	acnB	-15.18	0.018766	221 -- 251	38 -- 71	aconitate hydratase 2; aconitase B; 2-methyl-cis-aconitate hydratase	_____________220________________________________252\n_____________\|__________________________________\|\n5'-GAG...CGUAA_________UGAG___GC_______G________GCGCC...UGC-3'\n______________GCACGUAGC____CGU_____CGCU_AGGGGAUU\n______________:\|\|\|::\|\|\|____\|\|\|_____\|:\|\|_\|\|\|\|\|\|\|\|\n______________UGUGUGUCG____GCA_____GUGA_UCCCCUAA\n3'-UUU...UCUGA_________UUAA___ACAAA____G________AGGUA...GCA-5'\n_____________\|__________________________________\|\n_____________72_________________________________37\n	245 -- 251	38 -- 44	-33.2374 kcal/mol	13.3072 kcal/mol	4.74986 kcal/mol	220 252@LB@ \| \|@LB@5'-GAG...CGUAA UGAG GC G GCGCC...UGC-3'@LB@ GCACGUAGC CGU CGCU AGGGGAUU@LB@ :\|\|\|::\|\|\| \|\|\| \|:\|\| \|\|\|\|\|\|\|\|@LB@ UGUGUGUCG GCA GUGA UCCCCUAA@LB@3'-UUU...UCUGA UUAA ACAAA G AGGUA...GCA-5'@LB@ \| \|@LB@ 72 37@LB@@LB@@LB@Energy -15.18 kcal/mol@LB@Hybridization Energy -33.2374 kcal/mol@LB@Unfolding Energy - mRNA 13.3072 kcal/mol@LB@Unfolding Energy - sRNA 4.74986 kcal/mol@LB@@LB@Position - mRNA 221 -- 251@LB@Position - sRNA 38 -- 71@LB@Position Seed - mRNA 245 -- 251@LB@Position Seed - sRNA 38 -- 44@LB@
126	0.00562	0.157	b3017	ftsP	-15.30	0.017476	190 -- 242	38 -- 84	septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)	_____________189____________________________________________________243\n_____________\|______________________________________________________\|\n5'-GAC...UUUAC____A_____U__GU___U____CGG____CAG________GGCAUC_______GCACU...CGC-3'\n______________AUGG_GCAAA_AU__CAC_CAGU____CGU___UUCAUUCA______GGGGAUU\n______________\|\|\|\|_\|\|\|\|\|_\|:__\|\|\|_\|\|\|:____\|\|\|___\|\|\|\|:\|\|\|______\|\|\|\|\|\|\|\n______________UACC_CGUUU_UG__GUG_GUCG____GCA___AAGUGAGU______CCCCUAA\n3'-UUU...CCCUC__________C__AU___U____UUAA___ACA_____________________AGGUA...GCA-5'\n_____________\|______________________________________________________\|\n_____________85_____________________________________________________37\n	236 -- 242	38 -- 44	-40.4092 kcal/mol	11.1124 kcal/mol	13.9994 kcal/mol	189 243@LB@ \| \|@LB@5'-GAC...UUUAC A U GU U CGG CAG GGCAUC GCACU...CGC-3'@LB@ AUGG GCAAA AU CAC CAGU CGU UUCAUUCA GGGGAUU@LB@ \|\|\|\| \|\|\|\|\| \|: \|\|\| \|\|\|: \|\|\| \|\|\|\|:\|\|\| \|\|\|\|\|\|\|@LB@ UACC CGUUU UG GUG GUCG GCA AAGUGAGU CCCCUAA@LB@3'-UUU...CCCUC C AU U UUAA ACA AGGUA...GCA-5'@LB@ \| \|@LB@ 85 37@LB@@LB@@LB@Energy -15.30 kcal/mol@LB@Hybridization Energy -40.4092 kcal/mol@LB@Unfolding Energy - mRNA 11.1124 kcal/mol@LB@Unfolding Energy - sRNA 13.9994 kcal/mol@LB@@LB@Position - mRNA 190 -- 242@LB@Position - sRNA 38 -- 84@LB@Position Seed - mRNA 236 -- 242@LB@Position Seed - sRNA 38 -- 44@LB@
184	0.00937	0.1776	b2467	nudK	-13.30	0.054361	69 -- 94	38 -- 78	GDP-mannose pyrophosphatase	_____________68________________________________________95\n_____________\|_________________________________________\|\n5'-CAA...GGCAG______GU_________________________________AUUGA...AAG-3'\n______________AAGGAC__CAUGCGGCA_______________CAGGGGAUU\n______________\|\|:\|\|\|__\|\|::\|:\|\|\|_______________\|\|\|\|\|\|\|\|\|\n______________UUUCUG__GUGUGUCGU_______________GUCCCCUAA\n3'-UUU...ACCCG______AU_________UAAGCAACAAAGUGA_________AGGUA...GCA-5'\n_____________\|_________________________________________\|\n_____________79________________________________________37\n	88 -- 94	38 -- 44	-30.5001 kcal/mol	12.4248 kcal/mol	4.77973 kcal/mol	68 95@LB@ \| \|@LB@5'-CAA...GGCAG GU AUUGA...AAG-3'@LB@ AAGGAC CAUGCGGCA CAGGGGAUU@LB@ \|\|:\|\|\| \|\|::\|:\|\|\| \|\|\|\|\|\|\|\|\|@LB@ UUUCUG GUGUGUCGU GUCCCCUAA@LB@3'-UUU...ACCCG AU UAAGCAACAAAGUGA AGGUA...GCA-5'@LB@ \| \|@LB@ 79 37@LB@@LB@@LB@Energy -13.30 kcal/mol@LB@Hybridization Energy -30.5001 kcal/mol@LB@Unfolding Energy - mRNA 12.4248 kcal/mol@LB@Unfolding Energy - sRNA 4.77973 kcal/mol@LB@@LB@Position - mRNA 69 -- 94@LB@Position - sRNA 38 -- 78@LB@Position Seed - mRNA 88 -- 94@LB@Position Seed - sRNA 38 -- 44@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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