Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
61	0.002486	0.1417	b1487	ddpA	-13.46	0.049908	168 -- 192	43 -- 71	D D-dipeptide ABC transporter periplasmic binding protein	_____________167___________________________193\n_____________\|_____________________________\|\n5'-UCA...CCCUG________________CAG____A_____AAACU...UGA-3'\n______________GCACACAGCA___CGU____UUA_UCAGG\n______________:\|\|\|\|\|\|\|\|\|___\|\|\|____\|:\|_\|\|\|\|\|\n______________UGUGUGUCGU___GCA____AGU_AGUCC\n3'-UUU...UCUGA__________UAA___ACAA___G_____CCUAA...GCA-5'\n_____________\|_____________________________\|\n_____________72____________________________42\n	171 -- 177	62 -- 68	-25.043 kcal/mol	7.26137 kcal/mol	4.32409 kcal/mol	167 193@LB@ \| \|@LB@5'-UCA...CCCUG CAG A AAACU...UGA-3'@LB@ GCACACAGCA CGU UUA UCAGG@LB@ :\|\|\|\|\|\|\|\|\| \|\|\| \|:\| \|\|\|\|\|@LB@ UGUGUGUCGU GCA AGU AGUCC@LB@3'-UUU...UCUGA UAA ACAA G CCUAA...GCA-5'@LB@ \| \|@LB@ 72 42@LB@@LB@@LB@Energy -13.46 kcal/mol@LB@Hybridization Energy -25.043 kcal/mol@LB@Unfolding Energy - mRNA 7.26137 kcal/mol@LB@Unfolding Energy - sRNA 4.32409 kcal/mol@LB@@LB@Position - mRNA 168 -- 192@LB@Position - sRNA 43 -- 71@LB@Position Seed - mRNA 171 -- 177@LB@Position Seed - sRNA 62 -- 68@LB@
74	0.003066	0.1452	b0467	priC	-11.71	0.118543	157 -- 193	43 -- 75	primosomal replication protein N''	_____________156___________________________________194\n_____________\|_____________________________________\|\n5'-GGG...AGAGU______UUUA_____A_____AUCAU_______G___UCUUA...GUU-3'\n______________GGCUAC____GCAUA_CAAUU_____UUUCAUU_AGG\n______________\|:\|\|\|\|____:\|\|:\|_\|\|\|\|\|_____\|\|\|\|\|:\|_\|\|\|\n______________CUGAUG____UGUGU_GUUAA_____AAAGUGA_UCC\n3'-UUU...CGUUU_______________C_____GCAAC_______G___CCUAA...GCA-5'\n_____________\|_____________________________________\|\n_____________76____________________________________42\n	183 -- 189	47 -- 53	-24.815 kcal/mol	8.75149 kcal/mol	4.35266 kcal/mol	156 194@LB@ \| \|@LB@5'-GGG...AGAGU UUUA A AUCAU G UCUUA...GUU-3'@LB@ GGCUAC GCAUA CAAUU UUUCAUU AGG@LB@ \|:\|\|\|\| :\|\|:\| \|\|\|\|\| \|\|\|\|\|:\| \|\|\|@LB@ CUGAUG UGUGU GUUAA AAAGUGA UCC@LB@3'-UUU...CGUUU C GCAAC G CCUAA...GCA-5'@LB@ \| \|@LB@ 76 42@LB@@LB@@LB@Energy -11.71 kcal/mol@LB@Hybridization Energy -24.815 kcal/mol@LB@Unfolding Energy - mRNA 8.75149 kcal/mol@LB@Unfolding Energy - sRNA 4.35266 kcal/mol@LB@@LB@Position - mRNA 157 -- 193@LB@Position - sRNA 43 -- 75@LB@Position Seed - mRNA 183 -- 189@LB@Position Seed - sRNA 47 -- 53@LB@
78	0.003218	0.1452	b1641	slyB	-13.21	0.056987	203 -- 235	43 -- 75	outer membrane lipoprotein	_____________202_________________________________236\n_____________\|___________________________________\|\n5'-CCG...UCAAU_____A___________G_________AUGG____UCUGU...AAG-3'\n______________GAUUA_ACGC_GU_AUU_GUUGUUUCA____UAGG\n______________\|\|:\|\|_\|\|:\|_\|:_\|\|\|_\|\|\|\|\|\|\|\|\|____:\|\|\|\n______________CUGAU_UGUG_CG_UAA_CAACAAAGU____GUCC\n3'-UUU...CGUUU_____G____U__U___G_________GA______CCUAA...GCA-5'\n_____________\|___________________________________\|\n_____________76__________________________________42\n	221 -- 227	49 -- 55	-26.1882 kcal/mol	8.63028 kcal/mol	4.35266 kcal/mol	202 236@LB@ \| \|@LB@5'-CCG...UCAAU A G AUGG UCUGU...AAG-3'@LB@ GAUUA ACGC GU AUU GUUGUUUCA UAGG@LB@ \|\|:\|\| \|\|:\| \|: \|\|\| \|\|\|\|\|\|\|\|\| :\|\|\|@LB@ CUGAU UGUG CG UAA CAACAAAGU GUCC@LB@3'-UUU...CGUUU G U U G GA CCUAA...GCA-5'@LB@ \| \|@LB@ 76 42@LB@@LB@@LB@Energy -13.21 kcal/mol@LB@Hybridization Energy -26.1882 kcal/mol@LB@Unfolding Energy - mRNA 8.63028 kcal/mol@LB@Unfolding Energy - sRNA 4.35266 kcal/mol@LB@@LB@Position - mRNA 203 -- 235@LB@Position - sRNA 43 -- 75@LB@Position Seed - mRNA 221 -- 227@LB@Position Seed - sRNA 49 -- 55@LB@
114	0.005004	0.1545	b0025	ribF	-11.63	0.123109	210 -- 237	43 -- 71	bifunctional riboflavin kinase/FAD synthetase	_____________209___________________________238\n_____________\|_____________________________\|\n5'-UGG...AGCUG__________UACAUAAUC____GC____CCCCG...GCG-3'\n______________AUACGCGGCA__________UCA__CAGG\n______________\|:\|\|:\|:\|\|\|__________\|\|\|__\|\|\|\|\n______________UGUGUGUCGU__________AGU__GUCC\n3'-UUU...UCUGA__________UAAGCAACAA___GA____CCUAA...GCA-5'\n_____________\|_____________________________\|\n_____________72____________________________42\n	213 -- 219	62 -- 68	-21.1915 kcal/mol	5.23887 kcal/mol	4.32409 kcal/mol	209 238@LB@ \| \|@LB@5'-UGG...AGCUG UACAUAAUC GC CCCCG...GCG-3'@LB@ AUACGCGGCA UCA CAGG@LB@ \|:\|\|:\|:\|\|\| \|\|\| \|\|\|\|@LB@ UGUGUGUCGU AGU GUCC@LB@3'-UUU...UCUGA UAAGCAACAA GA CCUAA...GCA-5'@LB@ \| \|@LB@ 72 42@LB@@LB@@LB@Energy -11.63 kcal/mol@LB@Hybridization Energy -21.1915 kcal/mol@LB@Unfolding Energy - mRNA 5.23887 kcal/mol@LB@Unfolding Energy - sRNA 4.32409 kcal/mol@LB@@LB@Position - mRNA 210 -- 237@LB@Position - sRNA 43 -- 71@LB@Position Seed - mRNA 213 -- 219@LB@Position Seed - sRNA 62 -- 68@LB@
140	0.006445	0.1605	b0325	yahK	-13.93	0.038719	67 -- 79	43 -- 55	broad specificity NADPH-dependent aldehyde reductase Zn-containing	_____________66____________80\n_____________\|_____________\|\n5'-UUA...UGCUC_____________CUCGC...AAA-3'\n______________UGUUUCAUUCAGG\n______________\|\|\|\|\|\|\|:\|\|\|\|\|\n______________ACAAAGUGAGUCC\n3'-UUU...AAGCA_____________CCUAA...GCA-5'\n_____________\|_____________\|\n_____________56____________42\n	73 -- 79	43 -- 49	-18.9036 kcal/mol	3.47822 kcal/mol	1.49792 kcal/mol	66 80@LB@ \| \|@LB@5'-UUA...UGCUC CUCGC...AAA-3'@LB@ UGUUUCAUUCAGG@LB@ \|\|\|\|\|\|\|:\|\|\|\|\|@LB@ ACAAAGUGAGUCC@LB@3'-UUU...AAGCA CCUAA...GCA-5'@LB@ \| \|@LB@ 56 42@LB@@LB@@LB@Energy -13.93 kcal/mol@LB@Hybridization Energy -18.9036 kcal/mol@LB@Unfolding Energy - mRNA 3.47822 kcal/mol@LB@Unfolding Energy - sRNA 1.49792 kcal/mol@LB@@LB@Position - mRNA 67 -- 79@LB@Position - sRNA 43 -- 55@LB@Position Seed - mRNA 73 -- 79@LB@Position Seed - sRNA 43 -- 49@LB@
168	0.008282	0.1723	b3822	recQ	-12.50	0.081546	124 -- 155	43 -- 77	ATP-dependent DNA helicase	_____________123___________________________________156\n_____________\|_____________________________________\|\n5'-CGC...UCUCA_______AAA____U_______C_________G____CGCUG...UUA-3'\n______________GAGACUA___GCAU_GCAGUUU______UCGC_CAGG\n______________:\|\|\|\|\|\|___:\|\|:_\|\|\|:\|\|:______\|\|:\|_\|\|\|\|\n______________UUCUGAU___UGUG_CGUUAAG______AGUG_GUCC\n3'-UUU...CCCGU_______G______U_______CAACAA____A____CCUAA...GCA-5'\n_____________\|_____________________________________\|\n_____________78____________________________________42\n	139 -- 145	58 -- 64	-25.7534 kcal/mol	8.90299 kcal/mol	4.3531 kcal/mol	123 156@LB@ \| \|@LB@5'-CGC...UCUCA AAA U C G CGCUG...UUA-3'@LB@ GAGACUA GCAU GCAGUUU UCGC CAGG@LB@ :\|\|\|\|\|\| :\|\|: \|\|\|:\|\|: \|\|:\| \|\|\|\|@LB@ UUCUGAU UGUG CGUUAAG AGUG GUCC@LB@3'-UUU...CCCGU G U CAACAA A CCUAA...GCA-5'@LB@ \| \|@LB@ 78 42@LB@@LB@@LB@Energy -12.50 kcal/mol@LB@Hybridization Energy -25.7534 kcal/mol@LB@Unfolding Energy - mRNA 8.90299 kcal/mol@LB@Unfolding Energy - sRNA 4.3531 kcal/mol@LB@@LB@Position - mRNA 124 -- 155@LB@Position - sRNA 43 -- 77@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 58 -- 64@LB@
179	0.009001	0.1747	b2725	hycA	-11.58	0.125812	239 -- 264	43 -- 70	regulator of the transcriptional regulator FhlA	_____________238_____________________________265\n_____________\|_______________________________\|\n5'-UUA...UACAU__________CA____________CUA____ACCAG...AGG-3'\n______________CGCACAGCGG__UCG_____UCGC___CAGG\n______________\|:\|\|\|\|\|\|::__\|\|\|_____\|\|:\|___\|\|\|\|\n______________GUGUGUCGUU__AGC_____AGUG___GUCC\n3'-UUU...CUGAU__________A____AACAA____A______CCUAA...GCA-5'\n_____________\|_______________________________\|\n_____________71______________________________42\n	242 -- 248	61 -- 67	-23.0547 kcal/mol	7.15003 kcal/mol	4.32399 kcal/mol	238 265@LB@ \| \|@LB@5'-UUA...UACAU CA CUA ACCAG...AGG-3'@LB@ CGCACAGCGG UCG UCGC CAGG@LB@ \|:\|\|\|\|\|\|:: \|\|\| \|\|:\| \|\|\|\|@LB@ GUGUGUCGUU AGC AGUG GUCC@LB@3'-UUU...CUGAU A AACAA A CCUAA...GCA-5'@LB@ \| \|@LB@ 71 42@LB@@LB@@LB@Energy -11.58 kcal/mol@LB@Hybridization Energy -23.0547 kcal/mol@LB@Unfolding Energy - mRNA 7.15003 kcal/mol@LB@Unfolding Energy - sRNA 4.32399 kcal/mol@LB@@LB@Position - mRNA 239 -- 264@LB@Position - sRNA 43 -- 70@LB@Position Seed - mRNA 242 -- 248@LB@Position Seed - sRNA 61 -- 67@LB@
68	0.002787	0.1417	b3001	gpr	-15.31	0.017381	36 -- 59	44 -- 69	L-glyceraldehyde 3-phosphate reductase	_____________35__________________________60\n_____________\|___________________________\|\n5'-UCC...GUUAA________________CAC________UUGGU...GGC-3'\n______________ACGCGGCA__UCGUUG___UC_CUCAG\n______________\|\|:\|:\|\|\|__\|\|\|\|\|\|___\|\|_\|\|\|\|\|\n______________UGUGUCGU__AGCAAC___AG_GAGUC\n3'-UUU...UGAUG________UA______AA___U_____CCCUA...GCA-5'\n_____________\|___________________________\|\n_____________70__________________________43\n	37 -- 43	62 -- 68	-25.5933 kcal/mol	6.1914 kcal/mol	4.09554 kcal/mol	35 60@LB@ \| \|@LB@5'-UCC...GUUAA CAC UUGGU...GGC-3'@LB@ ACGCGGCA UCGUUG UC CUCAG@LB@ \|\|:\|:\|\|\| \|\|\|\|\|\| \|\| \|\|\|\|\|@LB@ UGUGUCGU AGCAAC AG GAGUC@LB@3'-UUU...UGAUG UA AA U CCCUA...GCA-5'@LB@ \| \|@LB@ 70 43@LB@@LB@@LB@Energy -15.31 kcal/mol@LB@Hybridization Energy -25.5933 kcal/mol@LB@Unfolding Energy - mRNA 6.1914 kcal/mol@LB@Unfolding Energy - sRNA 4.09554 kcal/mol@LB@@LB@Position - mRNA 36 -- 59@LB@Position - sRNA 44 -- 69@LB@Position Seed - mRNA 37 -- 43@LB@Position Seed - sRNA 62 -- 68@LB@
86	0.003687	0.1509	b0774	bioA	-10.95	0.166636	92 -- 152	44 -- 98	7 8-diaminopelargonic acid synthase PLP-dependent	_____________91_____________________________________________________________153\n_____________\|______________________________________________________________\|\n5'-ACG...ACAAU_____G______C____A__CAU______UCUCCAAA___U__UU_________A_______UGUAG...AAG-3'\n______________GUCCA_CGUGGG_GGUG_GC___GGGGCU________ACG_GU__UUUGUUGUU__AUUCGG\n______________\|:\|\|\|_\|\|\|\|\|\|_\|:\|\|_\|\|___::\|:\|\|________\|\|:_\|:__\|\|:\|\|\|\|\|\|__\|:\|\|:\|\n______________CGGGU_GCACCC_CUAC_CG___UUCUGA________UGU_CG__AAGCAACAA__UGAGUC\n____3'-UUUUUUU_____A______U____C__U________UGUG__________UU_________AG______CCCUA...GCA-5'\n_____________\|______________________________________________________________\|\n_____________99_____________________________________________________________43\n	139 -- 145	52 -- 58	-48.0349 kcal/mol	20.1482 kcal/mol	16.9429 kcal/mol	91 153@LB@ \| \|@LB@5'-ACG...ACAAU G C A CAU UCUCCAAA U UU A UGUAG...AAG-3'@LB@ GUCCA CGUGGG GGUG GC GGGGCU ACG GU UUUGUUGUU AUUCGG@LB@ \|:\|\|\| \|\|\|\|\|\| \|:\|\| \|\| ::\|:\|\| \|\|: \|: \|\|:\|\|\|\|\|\| \|:\|\|:\|@LB@ CGGGU GCACCC CUAC CG UUCUGA UGU CG AAGCAACAA UGAGUC@LB@ 3'-UUUUUUU A U C U UGUG UU AG CCCUA...GCA-5'@LB@ \| \|@LB@ 99 43@LB@@LB@@LB@Energy -10.95 kcal/mol@LB@Hybridization Energy -48.0349 kcal/mol@LB@Unfolding Energy - mRNA 20.1482 kcal/mol@LB@Unfolding Energy - sRNA 16.9429 kcal/mol@LB@@LB@Position - mRNA 92 -- 152@LB@Position - sRNA 44 -- 98@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 52 -- 58@LB@
93	0.004161	0.1538	b4221	tamB	-11.98	0.104509	154 -- 185	44 -- 78	translocation and assembly module for autotransporter export inner membrane subunit	_____________153__________________________________186\n_____________\|____________________________________\|\n5'-ACC...CGGAU______GA____________ACA________A____UCUGG...AUC-3'\n______________AAAGAC___ACACGG__GUU____GUUUUAC_UCGG\n______________\|\|\|\|\|\|___\|\|\|\|:\|__:\|\|____\|\|\|\|:\|\|_\|\|:\|\n______________UUUCUG___UGUGUC__UAA____CAAAGUG_AGUC\n3'-UUU...ACCCG______AUG______GU___GCAA____________CCCUA...GCA-5'\n_____________\|____________________________________\|\n_____________79___________________________________43\n	174 -- 180	48 -- 54	-22.2219 kcal/mol	6.10136 kcal/mol	4.13875 kcal/mol	153 186@LB@ \| \|@LB@5'-ACC...CGGAU GA ACA A UCUGG...AUC-3'@LB@ AAAGAC ACACGG GUU GUUUUAC UCGG@LB@ \|\|\|\|\|\| \|\|\|\|:\| :\|\| \|\|\|\|:\|\| \|\|:\|@LB@ UUUCUG UGUGUC UAA CAAAGUG AGUC@LB@3'-UUU...ACCCG AUG GU GCAA CCCUA...GCA-5'@LB@ \| \|@LB@ 79 43@LB@@LB@@LB@Energy -11.98 kcal/mol@LB@Hybridization Energy -22.2219 kcal/mol@LB@Unfolding Energy - mRNA 6.10136 kcal/mol@LB@Unfolding Energy - sRNA 4.13875 kcal/mol@LB@@LB@Position - mRNA 154 -- 185@LB@Position - sRNA 44 -- 78@LB@Position Seed - mRNA 174 -- 180@LB@Position Seed - sRNA 48 -- 54@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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