Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
110	0.004806	0.1538	b1429	tehA	-12.16	0.096068	32 -- 62	44 -- 75	potassium-tellurite ethidium and proflavin transporter	_____________31_________________________________63\n_____________\|__________________________________\|\n5'-AAU...UUCCU___A__UAAA_________C___UC_________ACGAU...UUU-3'\n______________GAU_AC____GGC___UCG_UGU__UUCACUUAG\n______________\|\|:_\|\|____:\|\|___\|\|\|_\|\|\|__\|\|\|\|\|\|:\|\|\n______________CUG_UG____UCG___AGC_ACA__AAGUGAGUC\n3'-UUU...CGUUU___A__UGUG___UUA___A______________CCCUA...GCA-5'\n_____________\|__________________________________\|\n_____________76_________________________________43\n	56 -- 62	44 -- 50	-20.5577 kcal/mol	4.26011 kcal/mol	4.13839 kcal/mol	31 63@LB@ \| \|@LB@5'-AAU...UUCCU A UAAA C UC ACGAU...UUU-3'@LB@ GAU AC GGC UCG UGU UUCACUUAG@LB@ \|\|: \|\| :\|\| \|\|\| \|\|\| \|\|\|\|\|\|:\|\|@LB@ CUG UG UCG AGC ACA AAGUGAGUC@LB@3'-UUU...CGUUU A UGUG UUA A CCCUA...GCA-5'@LB@ \| \|@LB@ 76 43@LB@@LB@@LB@Energy -12.16 kcal/mol@LB@Hybridization Energy -20.5577 kcal/mol@LB@Unfolding Energy - mRNA 4.26011 kcal/mol@LB@Unfolding Energy - sRNA 4.13839 kcal/mol@LB@@LB@Position - mRNA 32 -- 62@LB@Position - sRNA 44 -- 75@LB@Position Seed - mRNA 56 -- 62@LB@Position Seed - sRNA 44 -- 50@LB@
142	0.006549	0.1605	b3902	rhaD	-9.93	0.251242	192 -- 218	44 -- 69	rhamnulose-1-phosphate aldolase	_____________191_________________________219\n_____________\|___________________________\|\n5'-AGA...CAGGA______AACAUGCAAAACA________UCCUG...UGA-3'\n______________ACACAG_____________UUACUCAG\n______________\|\|\|\|\|\|_____________\|:\|\|\|\|\|\|\n______________UGUGUC_____________AGUGAGUC\n3'-UUU...UGAUG______GUUAAGCAACAA_________CCCUA...GCA-5'\n_____________\|___________________________\|\n_____________70__________________________43\n	212 -- 218	44 -- 50	-16.231 kcal/mol	2.20309 kcal/mol	4.09554 kcal/mol	191 219@LB@ \| \|@LB@5'-AGA...CAGGA AACAUGCAAAACA UCCUG...UGA-3'@LB@ ACACAG UUACUCAG@LB@ \|\|\|\|\|\| \|:\|\|\|\|\|\|@LB@ UGUGUC AGUGAGUC@LB@3'-UUU...UGAUG GUUAAGCAACAA CCCUA...GCA-5'@LB@ \| \|@LB@ 70 43@LB@@LB@@LB@Energy -9.93 kcal/mol@LB@Hybridization Energy -16.231 kcal/mol@LB@Unfolding Energy - mRNA 2.20309 kcal/mol@LB@Unfolding Energy - sRNA 4.09554 kcal/mol@LB@@LB@Position - mRNA 192 -- 218@LB@Position - sRNA 44 -- 69@LB@Position Seed - mRNA 212 -- 218@LB@Position Seed - sRNA 44 -- 50@LB@
154	0.007512	0.1668	b2180	yejF	-18.70	0.001576	262 -- 293	44 -- 72	microcin C ABC transporter ATPase	_____________261______________________________294\n_____________\|________________________________\|\n5'-GAC...AACCG_____________CAA__A_________A___AUUGAGG-3'\n______________UACGUACAGUAGU___UG_UGUUUCACU_CAG\n______________\|\|\|::\|\|\|\|:\|:\|___:\|_\|\|\|\|\|\|\|\|\|_\|\|\|\n______________AUGUGUGUCGUUA___GC_ACAAAGUGA_GUC\n3'-UUU...UUCUG_____________A____A_____________CCCUA...GCA-5'\n_____________\|________________________________\|\n_____________73_______________________________43\n	283 -- 289	47 -- 53	-27.8629 kcal/mol	5.03502 kcal/mol	4.12429 kcal/mol	261 294@LB@ \| \|@LB@5'-GAC...AACCG CAA A A AUUGAGG-3'@LB@ UACGUACAGUAGU UG UGUUUCACU CAG@LB@ \|\|\|::\|\|\|\|:\|:\| :\| \|\|\|\|\|\|\|\|\| \|\|\|@LB@ AUGUGUGUCGUUA GC ACAAAGUGA GUC@LB@3'-UUU...UUCUG A A CCCUA...GCA-5'@LB@ \| \|@LB@ 73 43@LB@@LB@@LB@Energy -18.70 kcal/mol@LB@Hybridization Energy -27.8629 kcal/mol@LB@Unfolding Energy - mRNA 5.03502 kcal/mol@LB@Unfolding Energy - sRNA 4.12429 kcal/mol@LB@@LB@Position - mRNA 262 -- 293@LB@Position - sRNA 44 -- 72@LB@Position Seed - mRNA 283 -- 289@LB@Position Seed - sRNA 47 -- 53@LB@
4	8.9e-06	0.006694	b3281	aroE	-13.87	0.039990	230 -- 259	45 -- 75	dehydroshikimate reductase NAD(P)-binding	_____________229_____________________________260\n_____________\|_______________________________\|\n5'-UUU...AAUCC___A__C________A___CCA_________UCAGC...AUG-3'\n______________GAU_GC_CACAGCAA_UCG____UUCAUUCA\n______________\|\|:_:\|_\|\|\|\|\|\|\|\|_\|\|\|____\|\|\|\|:\|\|\|\n______________CUG_UG_GUGUCGUU_AGC____AAGUGAGU\n3'-UUU...CGUUU___A__U________A___AACA________CCCCU...GCA-5'\n_____________\|_______________________________\|\n_____________76______________________________44\n	253 -- 259	45 -- 51	-26.2723 kcal/mol	8.66309 kcal/mol	3.74078 kcal/mol	229 260@LB@ \| \|@LB@5'-UUU...AAUCC A C A CCA UCAGC...AUG-3'@LB@ GAU GC CACAGCAA UCG UUCAUUCA@LB@ \|\|: :\| \|\|\|\|\|\|\|\| \|\|\| \|\|\|\|:\|\|\|@LB@ CUG UG GUGUCGUU AGC AAGUGAGU@LB@3'-UUU...CGUUU A U A AACA CCCCU...GCA-5'@LB@ \| \|@LB@ 76 44@LB@@LB@@LB@Energy -13.87 kcal/mol@LB@Hybridization Energy -26.2723 kcal/mol@LB@Unfolding Energy - mRNA 8.66309 kcal/mol@LB@Unfolding Energy - sRNA 3.74078 kcal/mol@LB@@LB@Position - mRNA 230 -- 259@LB@Position - sRNA 45 -- 75@LB@Position Seed - mRNA 253 -- 259@LB@Position Seed - sRNA 45 -- 51@LB@
8	3.984e-05	0.01752	b2752	cysD	-11.53	0.128779	103 -- 132	45 -- 74	sulfate adenylyltransferase subunit 2	_____________102____________________________133\n_____________\|______________________________\|\n5'-GCC...AAGGA_____U___UC_____G__AA_________UUCUC...UCU-3'\n______________ACUAC_CAU__UAAUU_GU__UUUCAUUCG\n______________\|\|\|\|\|_\|\|:__:\|\|\|\|_\|\|__\|\|\|\|\|:\|\|:\n______________UGAUG_GUG__GUUAA_CA__AAAGUGAGU\n3'-UUU...GUUUC_____U___UC_____G__AC_________CCCCU...GCA-5'\n_____________\|______________________________\|\n_____________75_____________________________44\n	126 -- 132	45 -- 51	-20.8412 kcal/mol	5.57115 kcal/mol	3.74056 kcal/mol	102 133@LB@ \| \|@LB@5'-GCC...AAGGA U UC G AA UUCUC...UCU-3'@LB@ ACUAC CAU UAAUU GU UUUCAUUCG@LB@ \|\|\|\|\| \|\|: :\|\|\|\| \|\| \|\|\|\|\|:\|\|:@LB@ UGAUG GUG GUUAA CA AAAGUGAGU@LB@3'-UUU...GUUUC U UC G AC CCCCU...GCA-5'@LB@ \| \|@LB@ 75 44@LB@@LB@@LB@Energy -11.53 kcal/mol@LB@Hybridization Energy -20.8412 kcal/mol@LB@Unfolding Energy - mRNA 5.57115 kcal/mol@LB@Unfolding Energy - sRNA 3.74056 kcal/mol@LB@@LB@Position - mRNA 103 -- 132@LB@Position - sRNA 45 -- 74@LB@Position Seed - mRNA 126 -- 132@LB@Position Seed - sRNA 45 -- 51@LB@
47	0.001402	0.105	b2297	pta	-18.38	0.002042	9 -- 40	45 -- 75	phosphate acetyltransferase	_____________8__________________________________41\n_____________\|__________________________________\|\n___5'-CGCAACCU_________UUU______A__UG___________ACAAA...GUC-3'\n______________GGCUGCACG___CGGCAA_UC___GUUUCA_UCA\n______________\|:\|\|:\|\|\|:___\|:\|\|\|\|_\|\|___\|\|\|\|\|\|_\|\|\|\n______________CUGAUGUGU___GUCGUU_AG___CAAAGU_AGU\n3'-UUU...CGUUU__________________A__CAA______G___CCCCU...GCA-5'\n_____________\|__________________________________\|\n_____________76_________________________________44\n	11 -- 17	67 -- 73	-26.9548 kcal/mol	4.83173 kcal/mol	3.74078 kcal/mol	8 41@LB@ \| \|@LB@ 5'-CGCAACCU UUU A UG ACAAA...GUC-3'@LB@ GGCUGCACG CGGCAA UC GUUUCA UCA@LB@ \|:\|\|:\|\|\|: \|:\|\|\|\| \|\| \|\|\|\|\|\| \|\|\|@LB@ CUGAUGUGU GUCGUU AG CAAAGU AGU@LB@3'-UUU...CGUUU A CAA G CCCCU...GCA-5'@LB@ \| \|@LB@ 76 44@LB@@LB@@LB@Energy -18.38 kcal/mol@LB@Hybridization Energy -26.9548 kcal/mol@LB@Unfolding Energy - mRNA 4.83173 kcal/mol@LB@Unfolding Energy - sRNA 3.74078 kcal/mol@LB@@LB@Position - mRNA 9 -- 40@LB@Position - sRNA 45 -- 75@LB@Position Seed - mRNA 11 -- 17@LB@Position Seed - sRNA 67 -- 73@LB@
90	0.004009	0.1538	b3152	yraR	-13.99	0.037452	47 -- 72	45 -- 75	putative nucleoside-diphosphate-sugar epimerase	_____________46______________________________73\n_____________\|_______________________________\|\n5'-ACU...UCAGU_____AA___________UC___________UGCUU...CGA-3'\n______________GAUUA___ACGGCAAUUU______UUACUCA\n______________\|\|:\|\|___\|\|:\|\|\|\|\|\|:______\|:\|\|\|\|\|\n______________CUGAU___UGUCGUUAAG______AGUGAGU\n3'-UUU...CGUUU_____GUG__________CAACAA_______CCCCU...GCA-5'\n_____________\|_______________________________\|\n_____________76______________________________44\n	66 -- 72	45 -- 51	-19.6514 kcal/mol	1.92306 kcal/mol	3.74078 kcal/mol	46 73@LB@ \| \|@LB@5'-ACU...UCAGU AA UC UGCUU...CGA-3'@LB@ GAUUA ACGGCAAUUU UUACUCA@LB@ \|\|:\|\| \|\|:\|\|\|\|\|\|: \|:\|\|\|\|\|@LB@ CUGAU UGUCGUUAAG AGUGAGU@LB@3'-UUU...CGUUU GUG CAACAA CCCCU...GCA-5'@LB@ \| \|@LB@ 76 44@LB@@LB@@LB@Energy -13.99 kcal/mol@LB@Hybridization Energy -19.6514 kcal/mol@LB@Unfolding Energy - mRNA 1.92306 kcal/mol@LB@Unfolding Energy - sRNA 3.74078 kcal/mol@LB@@LB@Position - mRNA 47 -- 72@LB@Position - sRNA 45 -- 75@LB@Position Seed - mRNA 66 -- 72@LB@Position Seed - sRNA 45 -- 51@LB@
118	0.005291	0.1553	b0481	ybaK	-9.02	0.349126	203 -- 225	45 -- 75	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase	_____________202_____________________________226\n_____________\|_______________________________\|\n5'-AAU...ACCAU______C______UAAA______________AAAAA...UCG-3'\n______________GAC_AC_CGCAGU___________UUACUCG\n______________\|\|\|_\|\|_\|:\|\|\|:___________\|:\|\|\|\|:\n______________CUG_UG_GUGUCG___________AGUGAGU\n3'-UUU...CGUUU___A__U______UUAAGCAACAA_______CCCCU...GCA-5'\n_____________\|_______________________________\|\n_____________76______________________________44\n	219 -- 225	45 -- 51	-15.8984 kcal/mol	3.13427 kcal/mol	3.74078 kcal/mol	202 226@LB@ \| \|@LB@5'-AAU...ACCAU C UAAA AAAAA...UCG-3'@LB@ GAC AC CGCAGU UUACUCG@LB@ \|\|\| \|\| \|:\|\|\|: \|:\|\|\|\|:@LB@ CUG UG GUGUCG AGUGAGU@LB@3'-UUU...CGUUU A U UUAAGCAACAA CCCCU...GCA-5'@LB@ \| \|@LB@ 76 44@LB@@LB@@LB@Energy -9.02 kcal/mol@LB@Hybridization Energy -15.8984 kcal/mol@LB@Unfolding Energy - mRNA 3.13427 kcal/mol@LB@Unfolding Energy - sRNA 3.74078 kcal/mol@LB@@LB@Position - mRNA 203 -- 225@LB@Position - sRNA 45 -- 75@LB@Position Seed - mRNA 219 -- 225@LB@Position Seed - sRNA 45 -- 51@LB@
139	0.006425	0.1605	b0924	mukB	-11.72	0.118276	203 -- 227	45 -- 75	chromosome condensin MukBEF ATPase and DNA-binding subunit	_____________202_____________________________228\n_____________\|_______________________________\|\n5'-UGG...AUAAU_____A_________A_______________CUGAC...CUG-3'\n______________GAUUG_ACGCGGUAA_______UUUCGCUCA\n______________\|\|:\|:_\|\|:\|:\|:\|\|_______\|\|\|\|:\|\|\|\|\n______________CUGAU_UGUGUCGUU_______AAAGUGAGU\n3'-UUU...CGUUU_____G_________AAGCAAC_________CCCCU...GCA-5'\n_____________\|_______________________________\|\n_____________76______________________________44\n	221 -- 227	45 -- 51	-22.3255 kcal/mol	6.8688 kcal/mol	3.74078 kcal/mol	202 228@LB@ \| \|@LB@5'-UGG...AUAAU A A CUGAC...CUG-3'@LB@ GAUUG ACGCGGUAA UUUCGCUCA@LB@ \|\|:\|: \|\|:\|:\|:\|\| \|\|\|\|:\|\|\|\|@LB@ CUGAU UGUGUCGUU AAAGUGAGU@LB@3'-UUU...CGUUU G AAGCAAC CCCCU...GCA-5'@LB@ \| \|@LB@ 76 44@LB@@LB@@LB@Energy -11.72 kcal/mol@LB@Hybridization Energy -22.3255 kcal/mol@LB@Unfolding Energy - mRNA 6.8688 kcal/mol@LB@Unfolding Energy - sRNA 3.74078 kcal/mol@LB@@LB@Position - mRNA 203 -- 227@LB@Position - sRNA 45 -- 75@LB@Position Seed - mRNA 221 -- 227@LB@Position Seed - sRNA 45 -- 51@LB@
152	0.007399	0.1668	b0591	entS	-13.87	0.039944	258 -- 287	45 -- 70	enterobactin exporter iron-regulated	_____________257____________________________288\n_____________\|______________________________\|\n5'-AAU...CGUUU_________UUCC___CUC______U____AUUGU...UGG-3'\n______________CGCGCAGUA____UCG___GUUUCA_CUCA\n______________\|:\|:\|\|\|:\|____\|\|\|___\|\|\|\|\|\|_\|\|\|\|\n______________GUGUGUCGU____AGC___CAAAGU_GAGU\n3'-UUU...CUGAU_________UA_____AA____________CCCCU...GCA-5'\n_____________\|______________________________\|\n_____________71_____________________________44\n	260 -- 266	62 -- 68	-23.1768 kcal/mol	5.59312 kcal/mol	3.71317 kcal/mol	257 288@LB@ \| \|@LB@5'-AAU...CGUUU UUCC CUC U AUUGU...UGG-3'@LB@ CGCGCAGUA UCG GUUUCA CUCA@LB@ \|:\|:\|\|\|:\| \|\|\| \|\|\|\|\|\| \|\|\|\|@LB@ GUGUGUCGU AGC CAAAGU GAGU@LB@3'-UUU...CUGAU UA AA CCCCU...GCA-5'@LB@ \| \|@LB@ 71 44@LB@@LB@@LB@Energy -13.87 kcal/mol@LB@Hybridization Energy -23.1768 kcal/mol@LB@Unfolding Energy - mRNA 5.59312 kcal/mol@LB@Unfolding Energy - sRNA 3.71317 kcal/mol@LB@@LB@Position - mRNA 258 -- 287@LB@Position - sRNA 45 -- 70@LB@Position Seed - mRNA 260 -- 266@LB@Position Seed - sRNA 62 -- 68@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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