Input and runtime details for job 8257533 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 8257533 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:34:31 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:34:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:30:38 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:30:45 CET 2018
	Job Completed		@ Fri Feb 16 22:31:01 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=8257533&toolName=CopraRNA ( 30 days expiry )

Description of the job

LhrA2

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003210 Listeria monocytogenes EGD-e

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
6	0.001395	0.4469	lmo2078	N/A	-19.77	0.011149	188 -- 240	154 -- 207		_____________187___________________________________________________________________241\n_____________\|_____________________________________________________________________\|\n5'-GAU...UUUAU_____C______U__________U____UA____________AAUG____A_G________________UUCGA...AGG-3'\n______________UUUGG_GCGUCU_UGU_____GG_AUGA__UGUAGA___GAA____AUGG_C_GGUGAGGU_AAUGGAA\n______________\|\|:\|\|_\|\|\|:\|\|_\|\|:_____\|\|_\|\|\|\|__::\|\|\|\|___\|\|\|____\|:\|\|_\|_::\|\|\|\|:\|_\|\|\|\|:\|\|\n______________AAGCC_CGCGGA_ACG_____CC_UACU__GUAUCU___CUU____UGCC_G_UUACUCUA_UUACUUU\n3'-GUU...AGUAC____________U___UAUUG__C____CC______GUA___GAUG______G________C_______UGUUU...GGA-5'\n_____________\|_____________________________________________________________________\|\n_____________208___________________________________________________________________153\n	200 -- 206	158 -- 164	-46.039 kcal/mol	12.924 kcal/mol	22.2029 kcal/mol	187 241@LB@ \| \|@LB@5'-GAU...UUUAU C U U UA AAUG A G UUCGA...AGG-3'@LB@ UUUGG GCGUCU UGU GG AUGA UGUAGA GAA AUGG C GGUGAGGU AAUGGAA@LB@ \|\|:\|\| \|\|\|:\|\| \|\|: \|\| \|\|\|\| ::\|\|\|\| \|\|\| \|:\|\| \| ::\|\|\|\|:\| \|\|\|\|:\|\|@LB@ AAGCC CGCGGA ACG CC UACU GUAUCU CUU UGCC G UUACUCUA UUACUUU@LB@3'-GUU...AGUAC U UAUUG C CC GUA GAUG G C UGUUU...GGA-5'@LB@ \| \|@LB@ 208 153@LB@@LB@@LB@Energy -19.77 kcal/mol@LB@Hybridization Energy -46.039 kcal/mol@LB@Unfolding Energy - mRNA 12.924 kcal/mol@LB@Unfolding Energy - sRNA 22.2029 kcal/mol@LB@@LB@Position - mRNA 188 -- 240@LB@Position - sRNA 154 -- 207@LB@Position Seed - mRNA 200 -- 206@LB@Position Seed - sRNA 158 -- 164@LB@
12	0.002933	0.4469	lmo1952	lysA	-19.68	0.011630	182 -- 245	154 -- 213	diaminopimelate decarboxylase	_____________181___________________________________________________________246\n_____________\|_____________________________________________________________\|\n5'-UUG...CUAUA_____AAGG______U__________________A______UG___ACA_______U____GCAGA...CGC-3'\n______________AUGUA____UGGGAU__GGCGU_GACGU___UUG_ACGGCU___GA___AUGAAUG_AAAU\n______________\|\|\|:\|____:\|\|\|\|\|__\|\|\|:\|_\|\|\|:\|___:\|:_\|\|\|\|\|:___\|\|___\|\|\|\|\|\|\|_\|\|\|:\n______________UACGU____GCCCUA__CCGUA_CUGUA___GAU_UGCCGG___CU___UACUUAC_UUUG\n3'-GUU...GCGGA_____AUU_______CU_____U_____CUU___G______UUA__C_________U____UUUAU...GGA-5'\n_____________\|_____________________________________________________________\|\n_____________214___________________________________________________________153\n	225 -- 231	161 -- 167	-39.877 kcal/mol	10.395 kcal/mol	19.5834 kcal/mol	181 246@LB@ \| \|@LB@5'-UUG...CUAUA AAGG U A UG ACA U GCAGA...CGC-3'@LB@ AUGUA UGGGAU GGCGU GACGU UUG ACGGCU GA AUGAAUG AAAU@LB@ \|\|\|:\| :\|\|\|\|\| \|\|\|:\| \|\|\|:\| :\|: \|\|\|\|\|: \|\| \|\|\|\|\|\|\| \|\|\|:@LB@ UACGU GCCCUA CCGUA CUGUA GAU UGCCGG CU UACUUAC UUUG@LB@3'-GUU...GCGGA AUU CU U CUU G UUA C U UUUAU...GGA-5'@LB@ \| \|@LB@ 214 153@LB@@LB@@LB@Energy -19.68 kcal/mol@LB@Hybridization Energy -39.877 kcal/mol@LB@Unfolding Energy - mRNA 10.395 kcal/mol@LB@Unfolding Energy - sRNA 19.5834 kcal/mol@LB@@LB@Position - mRNA 182 -- 245@LB@Position - sRNA 154 -- 213@LB@Position Seed - mRNA 225 -- 231@LB@Position Seed - sRNA 161 -- 167@LB@
29	0.005638	0.5232	lmo0014	qoxB	-19.61	0.012062	164 -- 215	168 -- 211	AA3-600 quinol oxidase subunit I	_____________163____________216\n_____________\|______________\|\n5'-CGG...CGCGA_____A________AAAUG...CUU-3'\n______________AGAUG_AAUGAAGA\n______________\|\|\|\|\|_\|\|\|\|\|\|:\|\n______________UCUAC_UUACUUUU\n3'-GUU...GUUAC______________GUUUA...GGA-5'\n_____________\|______________\|\n_____________212____________167\n	111 -- 117	157 -- 163	-11.9482 kcal/mol	0.56531 kcal/mol	0.884237 kcal/mol	163 216@LB@ \| \|@LB@5'-CGG...CGCGA A AAAUG...CUU-3'@LB@ AGAUG AAUGAAGA@LB@ \|\|\|\|\| \|\|\|\|\|\|:\|@LB@ UCUAC UUACUUUU@LB@3'-GUU...GUUAC GUUUA...GGA-5'@LB@ \| \|@LB@ 212 167@LB@@LB@@LB@Energy -19.61 kcal/mol@LB@Hybridization Energy -11.9482 kcal/mol@LB@Unfolding Energy - mRNA 0.56531 kcal/mol@LB@Unfolding Energy - sRNA 0.884237 kcal/mol@LB@@LB@Position - mRNA 164 -- 215@LB@Position - sRNA 168 -- 211@LB@Position Seed - mRNA 111 -- 117@LB@Position Seed - sRNA 157 -- 163@LB@
28	0.005366	0.5157	lmo2793	N/A	-19.39	0.013418	199 -- 253	121 -- 174	lmo2793	_____________198__________________254\n_____________\|____________________\|\n5'-AAA...UGUAG_________UUU________AUUGG...GUU-3'\n______________CAGUGAGGU___UGAAGAUA\n______________\|\|:\|\|\|\|:\|___\|\|\|\|:\|:\|\n______________GUUACUCUA___ACUUUUGU\n3'-GUU...UGCCG_________CUU________UUAUU...GGA-5'\n_____________\|____________________\|\n_____________175__________________120\n	212 -- 218	155 -- 161	-16.7389 kcal/mol	5.68665 kcal/mol	0.95817 kcal/mol	198 254@LB@ \| \|@LB@5'-AAA...UGUAG UUU AUUGG...GUU-3'@LB@ CAGUGAGGU UGAAGAUA@LB@ \|\|:\|\|\|\|:\| \|\|\|\|:\|:\|@LB@ GUUACUCUA ACUUUUGU@LB@3'-GUU...UGCCG CUU UUAUU...GGA-5'@LB@ \| \|@LB@ 175 120@LB@@LB@@LB@Energy -19.39 kcal/mol@LB@Hybridization Energy -16.7389 kcal/mol@LB@Unfolding Energy - mRNA 5.68665 kcal/mol@LB@Unfolding Energy - sRNA 0.95817 kcal/mol@LB@@LB@Position - mRNA 199 -- 253@LB@Position - sRNA 121 -- 174@LB@Position Seed - mRNA 212 -- 218@LB@Position Seed - sRNA 155 -- 161@LB@
52	0.01176	0.599	lmo0302	N/A	-19.32	0.013869	143 -- 287	107 -- 220	lmo0302	_____________142________________288\n_____________\|__________________\|\n5'-CGA...CAUAG_________A__U_____AAAAA...AUA-3'\n______________GAGAUGAAU_GA_GAUGA\n______________\|\|\|\|\|\|\|\|\|_\|\|_:\|::\|\n______________CUCUACUUA_CU_UUGUU\n3'-GUU...GGUUA____________U_____UAUUA...GGA-5'\n_____________\|__________________\|\n_____________221________________106\n	189 -- 195	162 -- 168	-14.2485 kcal/mol	1.08239 kcal/mol	0.933796 kcal/mol	142 288@LB@ \| \|@LB@5'-CGA...CAUAG A U AAAAA...AUA-3'@LB@ GAGAUGAAU GA GAUGA@LB@ \|\|\|\|\|\|\|\|\| \|\| :\|::\|@LB@ CUCUACUUA CU UUGUU@LB@3'-GUU...GGUUA U UAUUA...GGA-5'@LB@ \| \|@LB@ 221 106@LB@@LB@@LB@Energy -19.32 kcal/mol@LB@Hybridization Energy -14.2485 kcal/mol@LB@Unfolding Energy - mRNA 1.08239 kcal/mol@LB@Unfolding Energy - sRNA 0.933796 kcal/mol@LB@@LB@Position - mRNA 143 -- 287@LB@Position - sRNA 107 -- 220@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 162 -- 168@LB@
34	0.007076	0.552	lmo2678	N/A	-19.30	0.013995	184 -- 246	155 -- 207	XRE family transcriptional regulator	_____________183__________________________________________________________247\n_____________\|____________________________________________________________\|\n5'-GAA...AGACG____AG_____AA______A__AA______U___CUAAUUGUGGA__________U____UCAGU...AAG-3'\n______________GAGG__AUGGA__AUGAAC_GC__GCGGCU_GUG___________AGAUGAA_GA_GGCA\n______________\|\|\|\|__\|\|:\|\|__\|\|\|\|\|\|_:\|__:\|\|\|\|:_:\|\|___________\|\|\|\|\|\|\|_\|\|_::\|\|\n______________CUCC__UAUCU__UACUUG_UG__UGCCGG_UAC___________UCUACUU_CU_UUGU\n3'-GUU...CCCUA____G______G_______A__________U_____________________A__U____UUAUU...GGA-5'\n_____________\|____________________________________________________________\|\n_____________208__________________________________________________________154\n	233 -- 239	163 -- 169	-37.3867 kcal/mol	12.1272 kcal/mol	13.9822 kcal/mol	183 247@LB@ \| \|@LB@5'-GAA...AGACG AG AA A AA U CUAAUUGUGGA U UCAGU...AAG-3'@LB@ GAGG AUGGA AUGAAC GC GCGGCU GUG AGAUGAA GA GGCA@LB@ \|\|\|\| \|\|:\|\| \|\|\|\|\|\| :\| :\|\|\|\|: :\|\| \|\|\|\|\|\|\| \|\| ::\|\|@LB@ CUCC UAUCU UACUUG UG UGCCGG UAC UCUACUU CU UUGU@LB@3'-GUU...CCCUA G G A U A U UUAUU...GGA-5'@LB@ \| \|@LB@ 208 154@LB@@LB@@LB@Energy -19.30 kcal/mol@LB@Hybridization Energy -37.3867 kcal/mol@LB@Unfolding Energy - mRNA 12.1272 kcal/mol@LB@Unfolding Energy - sRNA 13.9822 kcal/mol@LB@@LB@Position - mRNA 184 -- 246@LB@Position - sRNA 155 -- 207@LB@Position Seed - mRNA 233 -- 239@LB@Position Seed - sRNA 163 -- 169@LB@
109	0.02745	0.6733	lmo0843	N/A	-19.30	0.014041	79 -- 127	106 -- 151		_____________78____________________________________________________128\n_____________\|_____________________________________________________\|\n5'-CCG...GUCCA____C_________________________AUAAUUU__U_____________CCCUG...CUG-3'\n______________AGUG_UUUUGGUG_CU_GAC_GAUGUUUGA_______CU__UUGCCAUUUUAU\n______________\|:\|\|_\|\|\|\|\|\|\|\|_\|\|_:\|\|_\|\|\|\|\|:\|\|\|_______\|\|__\|\|\|\|\|\|\|\|:\|\|\|\n______________UUAC_AAAACCAC_GA_UUG_CUACAGACU_______GA__AACGGUAAGAUA\n3'-GUU...GUUUA____U________A__U___U__________________UC____________ACCUU...GGA-5'\n_____________\|_____________________________________________________\|\n_____________152___________________________________________________105\n	118 -- 124	109 -- 115	-38.6588 kcal/mol	9.72702 kcal/mol	18.7291 kcal/mol	78 128@LB@ \| \|@LB@5'-CCG...GUCCA C AUAAUUU U CCCUG...CUG-3'@LB@ AGUG UUUUGGUG CU GAC GAUGUUUGA CU UUGCCAUUUUAU@LB@ \|:\|\| \|\|\|\|\|\|\|\| \|\| :\|\| \|\|\|\|\|:\|\|\| \|\| \|\|\|\|\|\|\|\|:\|\|\|@LB@ UUAC AAAACCAC GA UUG CUACAGACU GA AACGGUAAGAUA@LB@3'-GUU...GUUUA U A U U UC ACCUU...GGA-5'@LB@ \| \|@LB@ 152 105@LB@@LB@@LB@Energy -19.30 kcal/mol@LB@Hybridization Energy -38.6588 kcal/mol@LB@Unfolding Energy - mRNA 9.72702 kcal/mol@LB@Unfolding Energy - sRNA 18.7291 kcal/mol@LB@@LB@Position - mRNA 79 -- 127@LB@Position - sRNA 106 -- 151@LB@Position Seed - mRNA 118 -- 124@LB@Position Seed - sRNA 109 -- 115@LB@
14	0.00304	0.4469	lmo1187	N/A	-19.26	0.014311	139 -- 172	153 -- 183	ethanolamine utilization protein EutQ	_____________138_______________________________173\n_____________\|_________________________________\|\n5'-UAA...GGUAU____________CC__AU______GA_______AGCAA...UUU-3'\n______________CUAUAUGGCUAA__GA__GAUGAA__AAAAUAA\n______________\|\|\|:\|:\|\|\|:\|\|__\|\|__\|\|\|\|\|\|__\|\|\|\|:\|\|\n______________GAUGUGCCGGUU__CU__CUACUU__UUUUGUU\n3'-GUU...UACUU____________A___________AC_______UAUUA...GGA-5'\n_____________\|_________________________________\|\n_____________184_______________________________152\n	165 -- 171	154 -- 160	-25.2263 kcal/mol	3.98561 kcal/mol	9.539 kcal/mol	138 173@LB@ \| \|@LB@5'-UAA...GGUAU CC AU GA AGCAA...UUU-3'@LB@ CUAUAUGGCUAA GA GAUGAA AAAAUAA@LB@ \|\|\|:\|:\|\|\|:\|\| \|\| \|\|\|\|\|\| \|\|\|\|:\|\|@LB@ GAUGUGCCGGUU CU CUACUU UUUUGUU@LB@3'-GUU...UACUU A AC UAUUA...GGA-5'@LB@ \| \|@LB@ 184 152@LB@@LB@@LB@Energy -19.26 kcal/mol@LB@Hybridization Energy -25.2263 kcal/mol@LB@Unfolding Energy - mRNA 3.98561 kcal/mol@LB@Unfolding Energy - sRNA 9.539 kcal/mol@LB@@LB@Position - mRNA 139 -- 172@LB@Position - sRNA 153 -- 183@LB@Position Seed - mRNA 165 -- 171@LB@Position Seed - sRNA 154 -- 160@LB@
64	0.01603	0.6733	lmo0131	N/A	-19.25	0.014339	193 -- 248	149 -- 204		_____________192____________________________________________________249\n_____________\|______________________________________________________\|\n5'-UGU...AAAAG____G_____G______AUCUCGA______AAAU________C___UAG_____AUAGU...UCG-3'\n______________GAGG_AUA_A_AUGGAU_______GCACGG____AUGGGAUG_GAU___GAGAA\n______________\|\|\|\|_\|\|\|_\|_\|\|\|:\|:_______:\|\|\|\|\|____\|\|\|:\|\|\|\|_:\|\|___\|\|:\|\|\n______________CUCC_UAU_U_UACUUG_______UGUGCC____UACUCUAC_UUA___CUUUU\n3'-GUU...CCCUA____G___C_G______A____________GGU_____________________GUUUA...GGA-5'\n_____________\|______________________________________________________\|\n_____________205____________________________________________________148\n	225 -- 231	165 -- 171	-32.0138 kcal/mol	8.31689 kcal/mol	13.9574 kcal/mol	192 249@LB@ \| \|@LB@5'-UGU...AAAAG G G AUCUCGA AAAU C UAG AUAGU...UCG-3'@LB@ GAGG AUA A AUGGAU GCACGG AUGGGAUG GAU GAGAA@LB@ \|\|\|\| \|\|\| \| \|\|\|:\|: :\|\|\|\|\| \|\|\|:\|\|\|\| :\|\| \|\|:\|\|@LB@ CUCC UAU U UACUUG UGUGCC UACUCUAC UUA CUUUU@LB@3'-GUU...CCCUA G C G A GGU GUUUA...GGA-5'@LB@ \| \|@LB@ 205 148@LB@@LB@@LB@Energy -19.25 kcal/mol@LB@Hybridization Energy -32.0138 kcal/mol@LB@Unfolding Energy - mRNA 8.31689 kcal/mol@LB@Unfolding Energy - sRNA 13.9574 kcal/mol@LB@@LB@Position - mRNA 193 -- 248@LB@Position - sRNA 149 -- 204@LB@Position Seed - mRNA 225 -- 231@LB@Position Seed - sRNA 165 -- 171@LB@
160	0.04276	0.7192	lmo0415	N/A	-19.07	0.015641	183 -- 241	108 -- 171	endo-1 4-beta-xylanase	_____________182_________________________________________________________________242\n_____________\|___________________________________________________________________\|\n5'-UUU...UUUUC_________GU______UAAU____AAA__A____GA______________C_______________CAUUA...AGU-3'\n______________UGAGGUGAG_____GCA____GUGA___UU_GGUG__UUAGAC_____UUU_ACUAGUUGCCAUUCU\n______________\|\|\|\|:\|\|\|:_____:\|\|____:\|\|\|___\|\|_\|\|\|\|__:\|\|:\|\|_____\|:\|_\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\n______________ACUCUACUU_____UGU____UACU___AA_CCAC__GAUUUG_____AGA_UGAUCAACGGUAAGA\n3'-GUU...CGGUU_________ACUUU___UUAU____A____A____A_______UCUAC___C_______________UAACC...GGA-5'\n_____________\|___________________________________________________________________\|\n_____________172_________________________________________________________________107\n	235 -- 241	108 -- 114	-46.29 kcal/mol	15.9956 kcal/mol	19.2069 kcal/mol	182 242@LB@ \| \|@LB@5'-UUU...UUUUC GU UAAU AAA A GA C CAUUA...AGU-3'@LB@ UGAGGUGAG GCA GUGA UU GGUG UUAGAC UUU ACUAGUUGCCAUUCU@LB@ \|\|\|\|:\|\|\|: :\|\| :\|\|\| \|\| \|\|\|\| :\|\|:\|\| \|:\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|@LB@ ACUCUACUU UGU UACU AA CCAC GAUUUG AGA UGAUCAACGGUAAGA@LB@3'-GUU...CGGUU ACUUU UUAU A A A UCUAC C UAACC...GGA-5'@LB@ \| \|@LB@ 172 107@LB@@LB@@LB@Energy -19.07 kcal/mol@LB@Hybridization Energy -46.29 kcal/mol@LB@Unfolding Energy - mRNA 15.9956 kcal/mol@LB@Unfolding Energy - sRNA 19.2069 kcal/mol@LB@@LB@Position - mRNA 183 -- 241@LB@Position - sRNA 108 -- 171@LB@Position Seed - mRNA 235 -- 241@LB@Position Seed - sRNA 108 -- 114@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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