Input and runtime details for job 6038639 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6038639 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:35:57 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:19:35 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:35:30 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:35:37 CET 2018
	Job Completed		@ Fri Feb 16 21:35:51 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6038639&toolName=CopraRNA ( 30 days expiry )

Description of the job

IhtA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000117 Chlamydia trachomatis D/UW-3/CX

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
32	0.009822	0.2518	ct_649	ygfA	-6.64	0.172247	22 -- 28	80 -- 86	5-formyltetrahydrofolate cyclo-ligase	_____________21______29\n_____________\|_______\|\n5'-UAC...AAGUG_______CCGAG...AGC-3'\n______________AUGGCUA\n______________\|:\|\|\|\|\|\n______________UGCCGAU\n3'-UUU...CUCUA_______UGAGG...GAA-5'\n_____________\|_______\|\n_____________87______79\n	22 -- 28	80 -- 86	-8.81528 kcal/mol	1.11082 kcal/mol	1.06449 kcal/mol	21 29@LB@ \| \|@LB@5'-UAC...AAGUG CCGAG...AGC-3'@LB@ AUGGCUA@LB@ \|:\|\|\|\|\|@LB@ UGCCGAU@LB@3'-UUU...CUCUA UGAGG...GAA-5'@LB@ \| \|@LB@ 87 79@LB@@LB@@LB@Energy -6.64 kcal/mol@LB@Hybridization Energy -8.81528 kcal/mol@LB@Unfolding Energy - mRNA 1.11082 kcal/mol@LB@Unfolding Energy - sRNA 1.06449 kcal/mol@LB@@LB@Position - mRNA 22 -- 28@LB@Position - sRNA 80 -- 86@LB@Position Seed - mRNA 22 -- 28@LB@Position Seed - sRNA 80 -- 86@LB@
75	0.02725	0.29	ct_536	dnaQ_2	-6.54	0.180881	173 -- 186	18 -- 31	DNA polymerase III subunit epsilon	_____________172____________187\n_____________\|______________\|\n5'-AGA...GAUUU_____U________AGAAC...GGU-3'\n______________GAGCU_UUUUAUGG\n______________:\|\|\|\|_\|\|::\|\|\|\|\n______________UUCGA_AAGGUACC\n3'-UUU...CAGUC_____U________GCAAU...GAA-5'\n_____________\|______________\|\n_____________32_____________17\n	180 -- 186	18 -- 24	-14.3298 kcal/mol	2.92947 kcal/mol	4.86077 kcal/mol	172 187@LB@ \| \|@LB@5'-AGA...GAUUU U AGAAC...GGU-3'@LB@ GAGCU UUUUAUGG@LB@ :\|\|\|\| \|\|::\|\|\|\|@LB@ UUCGA AAGGUACC@LB@3'-UUU...CAGUC U GCAAU...GAA-5'@LB@ \| \|@LB@ 32 17@LB@@LB@@LB@Energy -6.54 kcal/mol@LB@Hybridization Energy -14.3298 kcal/mol@LB@Unfolding Energy - mRNA 2.92947 kcal/mol@LB@Unfolding Energy - sRNA 4.86077 kcal/mol@LB@@LB@Position - mRNA 173 -- 186@LB@Position - sRNA 18 -- 31@LB@Position Seed - mRNA 180 -- 186@LB@Position Seed - sRNA 18 -- 24@LB@
94	0.03394	0.2959	ct_249	N/A	-6.52	0.182571	223 -- 229	80 -- 86		_____________222_____230\n_____________\|_______\|\n5'-UUG...UGAUC_______UUGGG...CUG-3'\n______________AUGGCUG\n______________\|:\|\|\|\|:\n______________UGCCGAU\n3'-UUU...CUCUA_______UGAGG...GAA-5'\n_____________\|_______\|\n_____________87______79\n	223 -- 229	80 -- 86	-8.72307 kcal/mol	1.13798 kcal/mol	1.06449 kcal/mol	222 230@LB@ \| \|@LB@5'-UUG...UGAUC UUGGG...CUG-3'@LB@ AUGGCUG@LB@ \|:\|\|\|\|:@LB@ UGCCGAU@LB@3'-UUU...CUCUA UGAGG...GAA-5'@LB@ \| \|@LB@ 87 79@LB@@LB@@LB@Energy -6.52 kcal/mol@LB@Hybridization Energy -8.72307 kcal/mol@LB@Unfolding Energy - mRNA 1.13798 kcal/mol@LB@Unfolding Energy - sRNA 1.06449 kcal/mol@LB@@LB@Position - mRNA 223 -- 229@LB@Position - sRNA 80 -- 86@LB@Position Seed - mRNA 223 -- 229@LB@Position Seed - sRNA 80 -- 86@LB@
64	0.02465	0.29	ct_045	pepA	-6.49	0.184926	191 -- 205	17 -- 33	cytosol aminopeptidase	_____________190_______________206\n_____________\|_________________\|\n5'-UUU...AACAA_________________AUUAC...CUG-3'\n______________AGAGGUUAUU__GUGGU\n______________\|\|\|:\|:\|\|\|\|__:\|\|\|:\n______________UCUUCGAUAA__UACCG\n3'-UUU...CUCAG__________GG_____CAAUC...GAA-5'\n_____________\|_________________\|\n_____________34________________16\n	191 -- 197	27 -- 33	-15.0477 kcal/mol	3.57571 kcal/mol	4.97804 kcal/mol	190 206@LB@ \| \|@LB@5'-UUU...AACAA AUUAC...CUG-3'@LB@ AGAGGUUAUU GUGGU@LB@ \|\|\|:\|:\|\|\|\| :\|\|\|:@LB@ UCUUCGAUAA UACCG@LB@3'-UUU...CUCAG GG CAAUC...GAA-5'@LB@ \| \|@LB@ 34 16@LB@@LB@@LB@Energy -6.49 kcal/mol@LB@Hybridization Energy -15.0477 kcal/mol@LB@Unfolding Energy - mRNA 3.57571 kcal/mol@LB@Unfolding Energy - sRNA 4.97804 kcal/mol@LB@@LB@Position - mRNA 191 -- 205@LB@Position - sRNA 17 -- 33@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 27 -- 33@LB@
173	0.07335	0.3448	ct_543	hisS	-6.45	0.189015	1 -- 7	82 -- 88	histidine--tRNA ligase	______________1______8\n______________\|______\|\n___________5'-_______GUAUU...AGC-3'\n______________AUGCGGC\n______________\|\|:\|\|\|\|\n______________UAUGCCG\n3'-UUU...GCCUC_______AUUGA...GAA-5'\n_____________\|_______\|\n_____________89______81\n	1 -- 7	82 -- 88	-9.9342 kcal/mol	2.38776 kcal/mol	1.09753 kcal/mol	1 8@LB@ \| \|@LB@ 5'- GUAUU...AGC-3'@LB@ AUGCGGC@LB@ \|\|:\|\|\|\|@LB@ UAUGCCG@LB@3'-UUU...GCCUC AUUGA...GAA-5'@LB@ \| \|@LB@ 89 81@LB@@LB@@LB@Energy -6.45 kcal/mol@LB@Hybridization Energy -9.9342 kcal/mol@LB@Unfolding Energy - mRNA 2.38776 kcal/mol@LB@Unfolding Energy - sRNA 1.09753 kcal/mol@LB@@LB@Position - mRNA 1 -- 7@LB@Position - sRNA 82 -- 88@LB@Position Seed - mRNA 1 -- 7@LB@Position Seed - sRNA 82 -- 88@LB@
60	0.02363	0.29	ct_796	glyQ	-6.44	0.189870	236 -- 248	21 -- 33	glycine--tRNA ligase	_____________235___________249\n_____________\|_____________\|\n5'-UAG...AUGAU__C__________AACUU...AAG-3'\n______________GG_AGCUAUUCUA\n______________:\|_\|\|\|\|\|\|\|\|:\|\n______________UC_UCGAUAAGGU\n3'-UUU...CUCAG__U__________ACCGC...GAA-5'\n_____________\|_____________\|\n_____________34____________20\n	241 -- 247	22 -- 28	-13.5001 kcal/mol	2.33043 kcal/mol	4.73034 kcal/mol	235 249@LB@ \| \|@LB@5'-UAG...AUGAU C AACUU...AAG-3'@LB@ GG AGCUAUUCUA@LB@ :\| \|\|\|\|\|\|\|\|:\|@LB@ UC UCGAUAAGGU@LB@3'-UUU...CUCAG U ACCGC...GAA-5'@LB@ \| \|@LB@ 34 20@LB@@LB@@LB@Energy -6.44 kcal/mol@LB@Hybridization Energy -13.5001 kcal/mol@LB@Unfolding Energy - mRNA 2.33043 kcal/mol@LB@Unfolding Energy - sRNA 4.73034 kcal/mol@LB@@LB@Position - mRNA 236 -- 248@LB@Position - sRNA 21 -- 33@LB@Position Seed - mRNA 241 -- 247@LB@Position Seed - sRNA 22 -- 28@LB@
198	0.08843	0.3671	ct_257	N/A	-6.39	0.194258	76 -- 83	79 -- 86		_____________75_______84\n_____________\|________\|\n5'-CUA...GGCGG________CAGAA...UCA-3'\n______________AUGGCUGA\n______________\|:\|\|\|\|:\|\n______________UGCCGAUU\n3'-UUU...CUCUA________GAGGU...GAA-5'\n_____________\|________\|\n_____________87_______78\n	77 -- 83	79 -- 85	-9.5904 kcal/mol	2.10083 kcal/mol	1.09752 kcal/mol	75 84@LB@ \| \|@LB@5'-CUA...GGCGG CAGAA...UCA-3'@LB@ AUGGCUGA@LB@ \|:\|\|\|\|:\|@LB@ UGCCGAUU@LB@3'-UUU...CUCUA GAGGU...GAA-5'@LB@ \| \|@LB@ 87 78@LB@@LB@@LB@Energy -6.39 kcal/mol@LB@Hybridization Energy -9.5904 kcal/mol@LB@Unfolding Energy - mRNA 2.10083 kcal/mol@LB@Unfolding Energy - sRNA 1.09752 kcal/mol@LB@@LB@Position - mRNA 76 -- 83@LB@Position - sRNA 79 -- 86@LB@Position Seed - mRNA 77 -- 83@LB@Position Seed - sRNA 79 -- 85@LB@
97	0.03496	0.2959	ct_539	trxA	-6.38	0.195495	87 -- 94	80 -- 87	thioredoxin	_____________86_______95\n_____________\|________\|\n5'-AUG...GAGGU________ACUGG...UGC-3'\n______________UAUGGCUA\n______________\|\|:\|\|\|\|\|\n______________AUGCCGAU\n3'-UUU...CCUCU________UGAGG...GAA-5'\n_____________\|________\|\n_____________88_______79\n	88 -- 94	80 -- 86	-10.4005 kcal/mol	2.9242 kcal/mol	1.09752 kcal/mol	86 95@LB@ \| \|@LB@5'-AUG...GAGGU ACUGG...UGC-3'@LB@ UAUGGCUA@LB@ \|\|:\|\|\|\|\|@LB@ AUGCCGAU@LB@3'-UUU...CCUCU UGAGG...GAA-5'@LB@ \| \|@LB@ 88 79@LB@@LB@@LB@Energy -6.38 kcal/mol@LB@Hybridization Energy -10.4005 kcal/mol@LB@Unfolding Energy - mRNA 2.9242 kcal/mol@LB@Unfolding Energy - sRNA 1.09752 kcal/mol@LB@@LB@Position - mRNA 87 -- 94@LB@Position - sRNA 80 -- 87@LB@Position Seed - mRNA 88 -- 94@LB@Position Seed - sRNA 80 -- 86@LB@
56	0.02215	0.29	ct_104	fabI	-6.34	0.199539	263 -- 271	80 -- 88	enoyl-(acyl carrier protein) reductase	_____________262_______272\n_____________\|_________\|\n5'-UUU...AACGG_________GGGCA...CAG-3'\n______________GUAUGGCUG\n______________:\|\|:\|\|\|\|:\n______________UAUGCCGAU\n3'-UUU...GCCUC_________UGAGG...GAA-5'\n_____________\|_________\|\n_____________89________79\n	265 -- 271	80 -- 86	-10.7973 kcal/mol	3.36368 kcal/mol	1.09753 kcal/mol	262 272@LB@ \| \|@LB@5'-UUU...AACGG GGGCA...CAG-3'@LB@ GUAUGGCUG@LB@ :\|\|:\|\|\|\|:@LB@ UAUGCCGAU@LB@3'-UUU...GCCUC UGAGG...GAA-5'@LB@ \| \|@LB@ 89 79@LB@@LB@@LB@Energy -6.34 kcal/mol@LB@Hybridization Energy -10.7973 kcal/mol@LB@Unfolding Energy - mRNA 3.36368 kcal/mol@LB@Unfolding Energy - sRNA 1.09753 kcal/mol@LB@@LB@Position - mRNA 263 -- 271@LB@Position - sRNA 80 -- 88@LB@Position Seed - mRNA 265 -- 271@LB@Position Seed - sRNA 80 -- 86@LB@
44	0.01666	0.29	ct_438	rs7	-6.33	0.200160	214 -- 223	79 -- 88	30S ribosomal protein S7	_____________213________224\n_____________\|__________\|\n5'-GCA...ACGAC__________GAAGA...ACG-3'\n______________AUGCGGCUGA\n______________\|\|:\|\|\|\|\|:\|\n______________UAUGCCGAUU\n3'-UUU...GCCUC__________GAGGU...GAA-5'\n_____________\|__________\|\n_____________89_________78\n	217 -- 223	79 -- 85	-13.0034 kcal/mol	5.57631 kcal/mol	1.09753 kcal/mol	213 224@LB@ \| \|@LB@5'-GCA...ACGAC GAAGA...ACG-3'@LB@ AUGCGGCUGA@LB@ \|\|:\|\|\|\|\|:\|@LB@ UAUGCCGAUU@LB@3'-UUU...GCCUC GAGGU...GAA-5'@LB@ \| \|@LB@ 89 78@LB@@LB@@LB@Energy -6.33 kcal/mol@LB@Hybridization Energy -13.0034 kcal/mol@LB@Unfolding Energy - mRNA 5.57631 kcal/mol@LB@Unfolding Energy - sRNA 1.09753 kcal/mol@LB@@LB@Position - mRNA 214 -- 223@LB@Position - sRNA 79 -- 88@LB@Position Seed - mRNA 217 -- 223@LB@Position Seed - sRNA 79 -- 85@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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usability assessment

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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