Input and runtime details for job 6527768 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6527768 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:51 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:20 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:38:58 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:39:05 CET 2018
	Job Completed		@ Fri Feb 16 20:39:38 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6527768&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
112	0.01125	0.3104	b2515	ispG	-10.24	0.110168	132 -- 140	4 -- 12	1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase	_____________131_______141\n_____________\|_________\|\n5'-CGU...CAGGU_________GACCC...UGA-3'\n______________UGCGCGUCU\n______________\|\|\|\|\|\|\|\|\|\n______________ACGCGCAGA\n3'-UUU...UGUUU_________AAG-5'\n_____________\|_________\|\n_____________13________3\n	134 -- 140	4 -- 10	-16.4542 kcal/mol	5.04928 kcal/mol	1.16019 kcal/mol	131 141@LB@ \| \|@LB@5'-CGU...CAGGU GACCC...UGA-3'@LB@ UGCGCGUCU@LB@ \|\|\|\|\|\|\|\|\|@LB@ ACGCGCAGA@LB@3'-UUU...UGUUU AAG-5'@LB@ \| \|@LB@ 13 3@LB@@LB@@LB@Energy -10.24 kcal/mol@LB@Hybridization Energy -16.4542 kcal/mol@LB@Unfolding Energy - mRNA 5.04928 kcal/mol@LB@Unfolding Energy - sRNA 1.16019 kcal/mol@LB@@LB@Position - mRNA 132 -- 140@LB@Position - sRNA 4 -- 12@LB@Position Seed - mRNA 134 -- 140@LB@Position Seed - sRNA 4 -- 10@LB@
101	0.01005	0.3104	b3288	fmt	-10.27	0.109217	249 -- 255	5 -- 11	10-formyltetrahydrofolate:L-methionyl-tRNA(fMet) N-formyltransferase	_____________248_____256\n_____________\|_______\|\n5'-AUU...UUGCA_______AUCUC...UGU-3'\n______________GCGCGUC\n______________\|\|\|\|\|\|\|\n______________CGCGCAG\n3'-UUU...GUUUA_______AAAG-5'\n_____________\|_______\|\n_____________12______4\n	249 -- 255	5 -- 11	-14.5658 kcal/mol	3.18739 kcal/mol	1.11145 kcal/mol	248 256@LB@ \| \|@LB@5'-AUU...UUGCA AUCUC...UGU-3'@LB@ GCGCGUC@LB@ \|\|\|\|\|\|\|@LB@ CGCGCAG@LB@3'-UUU...GUUUA AAAG-5'@LB@ \| \|@LB@ 12 4@LB@@LB@@LB@Energy -10.27 kcal/mol@LB@Hybridization Energy -14.5658 kcal/mol@LB@Unfolding Energy - mRNA 3.18739 kcal/mol@LB@Unfolding Energy - sRNA 1.11145 kcal/mol@LB@@LB@Position - mRNA 249 -- 255@LB@Position - sRNA 5 -- 11@LB@Position Seed - mRNA 249 -- 255@LB@Position Seed - sRNA 5 -- 11@LB@
169	0.01667	0.3104	b3575	yiaK	-9.90	0.125874	198 -- 219	8 -- 29	2 3-diketo-L-gulonate reductase NADH-dependent	_____________197____________________220\n_____________\|______________________\|\n5'-UUU...AGCCU_______AA______UU__A__AGUUA...UCG-3'\n______________GGGAUGA__GUGACA__UG_GC\n______________\|\|\|\|\|\|\|__:\|:\|\|\|__\|\|_\|\|\n______________CCCUACU__UAUUGU__AC_CG\n3'-UUU...UAAGU_______AC______UU__G__CAGAAAG-5'\n_____________\|______________________\|\n_____________30_____________________7\n	198 -- 204	23 -- 29	-23.0827 kcal/mol	5.7681 kcal/mol	7.41331 kcal/mol	197 220@LB@ \| \|@LB@5'-UUU...AGCCU AA UU A AGUUA...UCG-3'@LB@ GGGAUGA GUGACA UG GC@LB@ \|\|\|\|\|\|\| :\|:\|\|\| \|\| \|\|@LB@ CCCUACU UAUUGU AC CG@LB@3'-UUU...UAAGU AC UU G CAGAAAG-5'@LB@ \| \|@LB@ 30 7@LB@@LB@@LB@Energy -9.90 kcal/mol@LB@Hybridization Energy -23.0827 kcal/mol@LB@Unfolding Energy - mRNA 5.7681 kcal/mol@LB@Unfolding Energy - sRNA 7.41331 kcal/mol@LB@@LB@Position - mRNA 198 -- 219@LB@Position - sRNA 8 -- 29@LB@Position Seed - mRNA 198 -- 204@LB@Position Seed - sRNA 23 -- 29@LB@
86	0.008729	0.3104	b4213	cpdB	-12.03	0.054140	68 -- 81	8 -- 21	2':3'-cyclic-nucleotide 2'-phosphodiesterase	_____________67_____________82\n_____________\|______________\|\n5'-UGA...CUAUC_U____________CAACU...AUA-3'\n______________G_UAAUGAAUGCGC\n______________\|_\|\|\|::\|\|\|\|\|\|\|\n______________C_AUUGUUUACGCG\n3'-UUU...UACUA_U____________CAGAAAG-5'\n_____________\|______________\|\n_____________22_____________7\n	75 -- 81	8 -- 14	-15.4826 kcal/mol	2.2212 kcal/mol	1.23638 kcal/mol	67 82@LB@ \| \|@LB@5'-UGA...CUAUC U CAACU...AUA-3'@LB@ G UAAUGAAUGCGC@LB@ \| \|\|\|::\|\|\|\|\|\|\|@LB@ C AUUGUUUACGCG@LB@3'-UUU...UACUA U CAGAAAG-5'@LB@ \| \|@LB@ 22 7@LB@@LB@@LB@Energy -12.03 kcal/mol@LB@Hybridization Energy -15.4826 kcal/mol@LB@Unfolding Energy - mRNA 2.2212 kcal/mol@LB@Unfolding Energy - sRNA 1.23638 kcal/mol@LB@@LB@Position - mRNA 68 -- 81@LB@Position - sRNA 8 -- 21@LB@Position Seed - mRNA 75 -- 81@LB@Position Seed - sRNA 8 -- 14@LB@
10	0.0002413	0.08171	b2594	rluD	-15.70	0.011720	48 -- 64	5 -- 21	23S rRNA pseudouridine(1911 1915 1917) synthase	_____________47________________65\n_____________\|_________________\|\n5'-CCC...CACCA____UUUC_________AUGAC...GUU-3'\n______________GAUA____AUGCGCGUC\n______________\|\|\|\|____\|\|\|\|\|\|\|\|\|\n______________CUAU____UACGCGCAG\n3'-UUU...UACUA____UGUU_________AAAG-5'\n_____________\|_________________\|\n_____________22________________4\n	58 -- 64	5 -- 11	-19.7285 kcal/mol	2.7828 kcal/mol	1.24955 kcal/mol	47 65@LB@ \| \|@LB@5'-CCC...CACCA UUUC AUGAC...GUU-3'@LB@ GAUA AUGCGCGUC@LB@ \|\|\|\| \|\|\|\|\|\|\|\|\|@LB@ CUAU UACGCGCAG@LB@3'-UUU...UACUA UGUU AAAG-5'@LB@ \| \|@LB@ 22 4@LB@@LB@@LB@Energy -15.70 kcal/mol@LB@Hybridization Energy -19.7285 kcal/mol@LB@Unfolding Energy - mRNA 2.7828 kcal/mol@LB@Unfolding Energy - sRNA 1.24955 kcal/mol@LB@@LB@Position - mRNA 48 -- 64@LB@Position - sRNA 5 -- 21@LB@Position Seed - mRNA 58 -- 64@LB@Position Seed - sRNA 5 -- 11@LB@
144	0.01383	0.3104	b1135	rluE	-11.03	0.080673	174 -- 189	5 -- 20	23S rRNA pseudouridine(2457) synthase	_____________173______________190\n_____________\|________________\|\n5'-UGU...CCGUC____A___________AUUAG...AAC-3'\n______________AUGA_AGAUGCGCGUU\n______________\|\|:\|_\|:\|\|\|\|\|\|\|\|:\n______________UAUU_UUUACGCGCAG\n3'-UUU...ACUAC____G___________AAAG-5'\n_____________\|________________\|\n_____________21_______________4\n	183 -- 189	5 -- 11	-17.9797 kcal/mol	5.69694 kcal/mol	1.24804 kcal/mol	173 190@LB@ \| \|@LB@5'-UGU...CCGUC A AUUAG...AAC-3'@LB@ AUGA AGAUGCGCGUU@LB@ \|\|:\| \|:\|\|\|\|\|\|\|\|:@LB@ UAUU UUUACGCGCAG@LB@3'-UUU...ACUAC G AAAG-5'@LB@ \| \|@LB@ 21 4@LB@@LB@@LB@Energy -11.03 kcal/mol@LB@Hybridization Energy -17.9797 kcal/mol@LB@Unfolding Energy - mRNA 5.69694 kcal/mol@LB@Unfolding Energy - sRNA 1.24804 kcal/mol@LB@@LB@Position - mRNA 174 -- 189@LB@Position - sRNA 5 -- 20@LB@Position Seed - mRNA 183 -- 189@LB@Position Seed - sRNA 5 -- 11@LB@
133	0.01287	0.3104	b3179	rlmE	-8.60	0.205247	24 -- 43	1 -- 17	23S rRNA U2552 2'-O-ribose methyltransferase SAM-dependent	_____________23___________________44\n_____________\|____________________\|\n5'-GCC...CUUGA__________CAGAC_____AGGUG...CCC-3'\n______________GCGGAUGUGC_____CUUUC\n______________:\|::\|\|\|:\|\|_____\|\|\|\|\|\n______________UGUUUACGCG_____GAAAG\n3'-UUU...ACUAU__________CA________-5'\n_____________\|___________________\|\n_____________18__________________1\n	27 -- 33	8 -- 14	-16.3409 kcal/mol	6.50016 kcal/mol	1.23994 kcal/mol	23 44@LB@ \| \|@LB@5'-GCC...CUUGA CAGAC AGGUG...CCC-3'@LB@ GCGGAUGUGC CUUUC@LB@ :\|::\|\|\|:\|\| \|\|\|\|\|@LB@ UGUUUACGCG GAAAG@LB@3'-UUU...ACUAU CA -5'@LB@ \| \|@LB@ 18 1@LB@@LB@@LB@Energy -8.60 kcal/mol@LB@Hybridization Energy -16.3409 kcal/mol@LB@Unfolding Energy - mRNA 6.50016 kcal/mol@LB@Unfolding Energy - sRNA 1.23994 kcal/mol@LB@@LB@Position - mRNA 24 -- 43@LB@Position - sRNA 1 -- 17@LB@Position Seed - mRNA 27 -- 33@LB@Position Seed - sRNA 8 -- 14@LB@
76	0.007084	0.3087	b4214	cysQ	-5.34	0.578728	11 -- 17	26 -- 32	3'(2') 5'-bisphosphate nucleotidase	_____________10______18\n_____________\|_______\|\n5'-AAC...AAUCA_______CAUCC...ACA-3'\n______________UCAGGGA\n______________\|\|\|\|\|\|\|\n______________AGUCCCU\n3'-UUU...ACUUA_______ACUAC...AAG-5'\n_____________\|_______\|\n_____________33______25\n	11 -- 17	26 -- 32	-11.6237 kcal/mol	0.931861 kcal/mol	5.35475 kcal/mol	10 18@LB@ \| \|@LB@5'-AAC...AAUCA CAUCC...ACA-3'@LB@ UCAGGGA@LB@ \|\|\|\|\|\|\|@LB@ AGUCCCU@LB@3'-UUU...ACUUA ACUAC...AAG-5'@LB@ \| \|@LB@ 33 25@LB@@LB@@LB@Energy -5.34 kcal/mol@LB@Hybridization Energy -11.6237 kcal/mol@LB@Unfolding Energy - mRNA 0.931861 kcal/mol@LB@Unfolding Energy - sRNA 5.35475 kcal/mol@LB@@LB@Position - mRNA 11 -- 17@LB@Position - sRNA 26 -- 32@LB@Position Seed - mRNA 11 -- 17@LB@Position Seed - sRNA 26 -- 32@LB@
151	0.01442	0.3104	b1395	paaH	-15.43	0.013105	189 -- 216	8 -- 31	3-hydroxyadipyl-CoA dehydrogenase NAD+-dependent	_____________188__________________________217\n_____________\|____________________________\|\n5'-GGC...GUUCA______AAUA_C________________AAACU...UGU-3'\n______________CGGGGA____G_AUGAUGAUAAAUGUGC\n______________\|:\|\|\|\|____\|_\|\|\|\|\|:\|:\|\|\|\|\|:\|\|\n______________GUCCCU____C_UACUAUUGUUUACGCG\n3'-UUU...CUUAA______A_____________________CAGAAAG-5'\n_____________\|____________________________\|\n_____________32___________________________7\n	210 -- 216	8 -- 14	-28.6523 kcal/mol	5.47369 kcal/mol	7.74551 kcal/mol	188 217@LB@ \| \|@LB@5'-GGC...GUUCA AAUA C AAACU...UGU-3'@LB@ CGGGGA G AUGAUGAUAAAUGUGC@LB@ \|:\|\|\|\| \| \|\|\|\|\|:\|:\|\|\|\|\|:\|\|@LB@ GUCCCU C UACUAUUGUUUACGCG@LB@3'-UUU...CUUAA A CAGAAAG-5'@LB@ \| \|@LB@ 32 7@LB@@LB@@LB@Energy -15.43 kcal/mol@LB@Hybridization Energy -28.6523 kcal/mol@LB@Unfolding Energy - mRNA 5.47369 kcal/mol@LB@Unfolding Energy - sRNA 7.74551 kcal/mol@LB@@LB@Position - mRNA 189 -- 216@LB@Position - sRNA 8 -- 31@LB@Position Seed - mRNA 210 -- 216@LB@Position Seed - sRNA 8 -- 14@LB@
39	0.002561	0.2224	b3845	fadA	-14.30	0.021162	112 -- 126	5 -- 20	3-ketoacyl-CoA thiolase (thiolase I)	_____________111______________127\n_____________\|________________\|\n5'-UCC...UCUGC________________AUAAC...AUU-3'\n______________GUAAUAAA_GCGCGUC\n______________:\|\|\|:\|\|\|_\|\|\|\|\|\|\|\n______________UAUUGUUU_CGCGCAG\n3'-UUU...ACUAC________A_______AAAG-5'\n_____________\|________________\|\n_____________21_______________4\n	120 -- 126	5 -- 11	-19.3309 kcal/mol	3.78617 kcal/mol	1.24804 kcal/mol	111 127@LB@ \| \|@LB@5'-UCC...UCUGC AUAAC...AUU-3'@LB@ GUAAUAAA GCGCGUC@LB@ :\|\|\|:\|\|\| \|\|\|\|\|\|\|@LB@ UAUUGUUU CGCGCAG@LB@3'-UUU...ACUAC A AAAG-5'@LB@ \| \|@LB@ 21 4@LB@@LB@@LB@Energy -14.30 kcal/mol@LB@Hybridization Energy -19.3309 kcal/mol@LB@Unfolding Energy - mRNA 3.78617 kcal/mol@LB@Unfolding Energy - sRNA 1.24804 kcal/mol@LB@@LB@Position - mRNA 112 -- 126@LB@Position - sRNA 5 -- 20@LB@Position Seed - mRNA 120 -- 126@LB@Position Seed - sRNA 5 -- 11@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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usability assessment

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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