Input and runtime details for job 6527768 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6527768 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:51 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:20 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:38:58 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:39:05 CET 2018
	Job Completed		@ Fri Feb 16 20:39:38 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6527768&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
130	0.01263	0.3104	b1952	dsrB	-7.17	0.338083	230 -- 240	7 -- 18	uncharacterized protein	_____________229__________241\n_____________\|____________\|\n5'-CGU...GUCAA____________GUCCG...ACC-3'\n______________AACGGAUG_GCG\n______________\|\|\|::\|\|\|_\|\|\|\n______________UUGUUUAC_CGC\n3'-UUU...UACUA________G___AGAAAG-5'\n_____________\|____________\|\n_____________19___________6\n	231 -- 237	11 -- 17	-11.9456 kcal/mol	3.57064 kcal/mol	1.20891 kcal/mol	229 241@LB@ \| \|@LB@5'-CGU...GUCAA GUCCG...ACC-3'@LB@ AACGGAUG GCG@LB@ \|\|\|::\|\|\| \|\|\|@LB@ UUGUUUAC CGC@LB@3'-UUU...UACUA G AGAAAG-5'@LB@ \| \|@LB@ 19 6@LB@@LB@@LB@Energy -7.17 kcal/mol@LB@Hybridization Energy -11.9456 kcal/mol@LB@Unfolding Energy - mRNA 3.57064 kcal/mol@LB@Unfolding Energy - sRNA 1.20891 kcal/mol@LB@@LB@Position - mRNA 230 -- 240@LB@Position - sRNA 7 -- 18@LB@Position Seed - mRNA 231 -- 237@LB@Position Seed - sRNA 11 -- 17@LB@
191	0.01886	0.3104	b3494	uspB	-6.32	0.440313	280 -- 286	5 -- 11	universal stress (ethanol tolerance) protein B	_____________279_____287\n_____________\|_______\|\n5'-AAU...ACUAC_______GUUAG...UGC-3'\n______________GCGCGUU\n______________\|\|\|\|\|\|:\n______________CGCGCAG\n3'-UUU...GUUUA_______AAAG-5'\n_____________\|_______\|\n_____________12______4\n	280 -- 286	5 -- 11	-11.0709 kcal/mol	3.63469 kcal/mol	1.11145 kcal/mol	279 287@LB@ \| \|@LB@5'-AAU...ACUAC GUUAG...UGC-3'@LB@ GCGCGUU@LB@ \|\|\|\|\|\|:@LB@ CGCGCAG@LB@3'-UUU...GUUUA AAAG-5'@LB@ \| \|@LB@ 12 4@LB@@LB@@LB@Energy -6.32 kcal/mol@LB@Hybridization Energy -11.0709 kcal/mol@LB@Unfolding Energy - mRNA 3.63469 kcal/mol@LB@Unfolding Energy - sRNA 1.11145 kcal/mol@LB@@LB@Position - mRNA 280 -- 286@LB@Position - sRNA 5 -- 11@LB@Position Seed - mRNA 280 -- 286@LB@Position Seed - sRNA 5 -- 11@LB@
62	0.005646	0.3084	b1242	ychE	-13.92	0.024785	141 -- 151	1 -- 11	UPF0056 family inner membrane protein	_____________140_________152\n_____________\|___________\|\n5'-CAU...UGUCA___________CAACC...GCA-3'\n______________GCGCGUCUUUU\n______________\|\|\|\|\|\|\|\|\|\|:\n______________CGCGCAGAAAG\n3'-UUU...GUUUA___________-5'\n_____________\|__________\|\n_____________12_________1\n	145 -- 151	1 -- 7	-17.9236 kcal/mol	2.83374 kcal/mol	1.17076 kcal/mol	140 152@LB@ \| \|@LB@5'-CAU...UGUCA CAACC...GCA-3'@LB@ GCGCGUCUUUU@LB@ \|\|\|\|\|\|\|\|\|\|:@LB@ CGCGCAGAAAG@LB@3'-UUU...GUUUA -5'@LB@ \| \|@LB@ 12 1@LB@@LB@@LB@Energy -13.92 kcal/mol@LB@Hybridization Energy -17.9236 kcal/mol@LB@Unfolding Energy - mRNA 2.83374 kcal/mol@LB@Unfolding Energy - sRNA 1.17076 kcal/mol@LB@@LB@Position - mRNA 141 -- 151@LB@Position - sRNA 1 -- 11@LB@Position Seed - mRNA 145 -- 151@LB@Position Seed - sRNA 1 -- 7@LB@
19	0.0006629	0.1181	b2909	ygfB	-11.13	0.077732	186 -- 206	4 -- 22	UPF0149 family protein	_____________185___________________207\n_____________\|_____________________\|\n5'-AAA...CCCUU___C_________UUA_____AUACA...UCA-3'\n______________UGA_GACGAAUGC___UGUCU\n______________\|\|\|_:\|\|:\|\|\|\|\|___:\|\|\|\|\n______________ACU_UUGUUUACG___GCAGA\n3'-UUU...CUACU___A_________C_______AAG-5'\n_____________\|_____________________\|\n_____________23____________________3\n	192 -- 198	10 -- 16	-17.2827 kcal/mol	4.89762 kcal/mol	1.2572 kcal/mol	185 207@LB@ \| \|@LB@5'-AAA...CCCUU C UUA AUACA...UCA-3'@LB@ UGA GACGAAUGC UGUCU@LB@ \|\|\| :\|\|:\|\|\|\|\| :\|\|\|\|@LB@ ACU UUGUUUACG GCAGA@LB@3'-UUU...CUACU A C AAG-5'@LB@ \| \|@LB@ 23 3@LB@@LB@@LB@Energy -11.13 kcal/mol@LB@Hybridization Energy -17.2827 kcal/mol@LB@Unfolding Energy - mRNA 4.89762 kcal/mol@LB@Unfolding Energy - sRNA 1.2572 kcal/mol@LB@@LB@Position - mRNA 186 -- 206@LB@Position - sRNA 4 -- 22@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 10 -- 16@LB@
139	0.01337	0.3104	b1255	yciC	-11.25	0.074167	287 -- 299	1 -- 13	UPF0259 family inner membrane protein	_____________286___________300\n_____________\|_____________\|\n5'-AAU...UUGCU__________A__A-3'\n______________AUGUGCGUUU_UC\n______________\|\|\|:\|\|\|\|:\|_\|\|\n______________UACGCGCAGA_AG\n3'-UUU...UUGUU__________A__-5'\n_____________\|____________\|\n_____________14___________1\n	290 -- 296	4 -- 10	-15.0519 kcal/mol	2.63141 kcal/mol	1.17519 kcal/mol	286 300@LB@ \| \|@LB@5'-AAU...UUGCU A A-3'@LB@ AUGUGCGUUU UC@LB@ \|\|\|:\|\|\|\|:\| \|\|@LB@ UACGCGCAGA AG@LB@3'-UUU...UUGUU A -5'@LB@ \| \|@LB@ 14 1@LB@@LB@@LB@Energy -11.25 kcal/mol@LB@Hybridization Energy -15.0519 kcal/mol@LB@Unfolding Energy - mRNA 2.63141 kcal/mol@LB@Unfolding Energy - sRNA 1.17519 kcal/mol@LB@@LB@Position - mRNA 287 -- 299@LB@Position - sRNA 1 -- 13@LB@Position Seed - mRNA 290 -- 296@LB@Position Seed - sRNA 4 -- 10@LB@
177	0.01751	0.3104	b2900	yqfB	-9.18	0.165418	52 -- 70	1 -- 17	UPF0267 family protein	_____________51__________________71\n_____________\|___________________\|\n5'-UUC...ACGUC________U__GCC_____CCGCC...UCA-3'\n______________ACAAAUGC_CG___CUUUU\n______________\|\|\|\|\|\|\|\|_\|\|___\|\|\|\|:\n______________UGUUUACG_GC___GAAAG\n3'-UUU...ACUAU________C__A_______-5'\n_____________\|__________________\|\n_____________18_________________1\n	53 -- 59	10 -- 16	-13.5578 kcal/mol	3.13415 kcal/mol	1.23994 kcal/mol	51 71@LB@ \| \|@LB@5'-UUC...ACGUC U GCC CCGCC...UCA-3'@LB@ ACAAAUGC CG CUUUU@LB@ \|\|\|\|\|\|\|\| \|\| \|\|\|\|:@LB@ UGUUUACG GC GAAAG@LB@3'-UUU...ACUAU C A -5'@LB@ \| \|@LB@ 18 1@LB@@LB@@LB@Energy -9.18 kcal/mol@LB@Hybridization Energy -13.5578 kcal/mol@LB@Unfolding Energy - mRNA 3.13415 kcal/mol@LB@Unfolding Energy - sRNA 1.23994 kcal/mol@LB@@LB@Position - mRNA 52 -- 70@LB@Position - sRNA 1 -- 17@LB@Position Seed - mRNA 53 -- 59@LB@Position Seed - sRNA 10 -- 16@LB@
157	0.01467	0.3104	b0906	ycaP	-12.44	0.045773	54 -- 69	5 -- 18	UPF0702 family putative inner membrane protein	_____________53_______________70\n_____________\|________________\|\n5'-AGU...CCGGG_________UU_____AUGAA...UCU-3'\n______________GAUAAAUGU__GCGUC\n______________:\|:\|\|\|\|\|:__\|\|\|\|\|\n______________UUGUUUACG__CGCAG\n3'-UUU...UACUA________________AAAG-5'\n_____________\|________________\|\n_____________19_______________4\n	56 -- 62	10 -- 16	-16.0437 kcal/mol	2.38303 kcal/mol	1.2243 kcal/mol	53 70@LB@ \| \|@LB@5'-AGU...CCGGG UU AUGAA...UCU-3'@LB@ GAUAAAUGU GCGUC@LB@ :\|:\|\|\|\|\|: \|\|\|\|\|@LB@ UUGUUUACG CGCAG@LB@3'-UUU...UACUA AAAG-5'@LB@ \| \|@LB@ 19 4@LB@@LB@@LB@Energy -12.44 kcal/mol@LB@Hybridization Energy -16.0437 kcal/mol@LB@Unfolding Energy - mRNA 2.38303 kcal/mol@LB@Unfolding Energy - sRNA 1.2243 kcal/mol@LB@@LB@Position - mRNA 54 -- 69@LB@Position - sRNA 5 -- 18@LB@Position Seed - mRNA 56 -- 62@LB@Position Seed - sRNA 10 -- 16@LB@
146	0.01406	0.3104	b3831	udp	-7.35	0.318150	153 -- 161	1 -- 9	uridine phosphorylase	_____________152_______162\n_____________\|_________\|\n5'-AUC...GUAAA_________UGCUU...AGA-3'\n______________GUGUCUUUU\n______________\|:\|\|\|\|\|\|:\n______________CGCAGAAAG\n3'-UUU...UUACG_________-5'\n_____________\|________\|\n_____________10_______1\n	155 -- 161	1 -- 7	-10.4616 kcal/mol	1.99462 kcal/mol	1.11751 kcal/mol	152 162@LB@ \| \|@LB@5'-AUC...GUAAA UGCUU...AGA-3'@LB@ GUGUCUUUU@LB@ \|:\|\|\|\|\|\|:@LB@ CGCAGAAAG@LB@3'-UUU...UUACG -5'@LB@ \| \|@LB@ 10 1@LB@@LB@@LB@Energy -7.35 kcal/mol@LB@Hybridization Energy -10.4616 kcal/mol@LB@Unfolding Energy - mRNA 1.99462 kcal/mol@LB@Unfolding Energy - sRNA 1.11751 kcal/mol@LB@@LB@Position - mRNA 153 -- 161@LB@Position - sRNA 1 -- 9@LB@Position Seed - mRNA 155 -- 161@LB@Position Seed - sRNA 1 -- 7@LB@
161	0.01501	0.3104	b1709	btuD	-8.32	0.227073	129 -- 137	1 -- 9	vitamin B12 ABC transporter ATPase	_____________128_______138\n_____________\|_________\|\n5'-GCA...AUCCA_________CCCGG...GGC-3'\n______________GCGUUUUUC\n______________\|\|\|\|:\|\|\|\|\n______________CGCAGAAAG\n3'-UUU...UUACG_________-5'\n_____________\|________\|\n_____________10_______1\n	131 -- 137	1 -- 7	-11.771 kcal/mol	2.3321 kcal/mol	1.11751 kcal/mol	128 138@LB@ \| \|@LB@5'-GCA...AUCCA CCCGG...GGC-3'@LB@ GCGUUUUUC@LB@ \|\|\|\|:\|\|\|\|@LB@ CGCAGAAAG@LB@3'-UUU...UUACG -5'@LB@ \| \|@LB@ 10 1@LB@@LB@@LB@Energy -8.32 kcal/mol@LB@Hybridization Energy -11.771 kcal/mol@LB@Unfolding Energy - mRNA 2.3321 kcal/mol@LB@Unfolding Energy - sRNA 1.11751 kcal/mol@LB@@LB@Position - mRNA 129 -- 137@LB@Position - sRNA 1 -- 9@LB@Position Seed - mRNA 131 -- 137@LB@Position Seed - sRNA 1 -- 7@LB@
50	0.004415	0.299	b0752	zitB	-14.03	0.023662	243 -- 258	5 -- 21	zinc efflux system	_____________242_______________259\n_____________\|_________________\|\n5'-CGC...CAGAA____________U____GCUUG...UGG-3'\n______________GAUAAUAA_UGC_CGUC\n______________\|\|\|\|\|:\|\|_\|\|\|_\|\|\|\|\n______________CUAUUGUU_ACG_GCAG\n3'-UUU...UACUA________U___C____AAAG-5'\n_____________\|_________________\|\n_____________22________________4\n	244 -- 250	14 -- 20	-17.36 kcal/mol	2.08049 kcal/mol	1.24955 kcal/mol	242 259@LB@ \| \|@LB@5'-CGC...CAGAA U GCUUG...UGG-3'@LB@ GAUAAUAA UGC CGUC@LB@ \|\|\|\|\|:\|\| \|\|\| \|\|\|\|@LB@ CUAUUGUU ACG GCAG@LB@3'-UUU...UACUA U C AAAG-5'@LB@ \| \|@LB@ 22 4@LB@@LB@@LB@Energy -14.03 kcal/mol@LB@Hybridization Energy -17.36 kcal/mol@LB@Unfolding Energy - mRNA 2.08049 kcal/mol@LB@Unfolding Energy - sRNA 1.24955 kcal/mol@LB@@LB@Position - mRNA 243 -- 258@LB@Position - sRNA 5 -- 21@LB@Position Seed - mRNA 244 -- 250@LB@Position Seed - sRNA 14 -- 20@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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