Input and runtime details for job 6527768 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6527768 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:51 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:20 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:38:58 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:39:05 CET 2018
	Job Completed		@ Fri Feb 16 20:39:38 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6527768&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
124	0.0124	0.3104	b1860	ruvB	-8.20	0.236824	252 -- 265	8 -- 20	ATP-dependent DNA helicase component of RuvABC resolvasome	_____________251____________266\n_____________\|______________\|\n5'-ACG...AAGAU_________C____CAUUC...GUC-3'\n______________GUAGCAGAU_GCGC\n______________:\|\|:\|\|:\|\|_\|\|\|\|\n______________UAUUGUUUA_CGCG\n3'-UUU...ACUAC______________CAGAAAG-5'\n_____________\|______________\|\n_____________21_____________7\n	252 -- 258	14 -- 20	-14.7986 kcal/mol	5.36112 kcal/mol	1.23367 kcal/mol	251 266@LB@ \| \|@LB@5'-ACG...AAGAU C CAUUC...GUC-3'@LB@ GUAGCAGAU GCGC@LB@ :\|\|:\|\|:\|\| \|\|\|\|@LB@ UAUUGUUUA CGCG@LB@3'-UUU...ACUAC CAGAAAG-5'@LB@ \| \|@LB@ 21 7@LB@@LB@@LB@Energy -8.20 kcal/mol@LB@Hybridization Energy -14.7986 kcal/mol@LB@Unfolding Energy - mRNA 5.36112 kcal/mol@LB@Unfolding Energy - sRNA 1.23367 kcal/mol@LB@@LB@Position - mRNA 252 -- 265@LB@Position - sRNA 8 -- 20@LB@Position Seed - mRNA 252 -- 258@LB@Position Seed - sRNA 14 -- 20@LB@
84	0.008129	0.3104	b2579	grcA	-9.71	0.135356	279 -- 291	5 -- 18	autonomous glycyl radical cofactor	_____________278____________292\n_____________\|______________\|\n5'-UUG...AAAAA______________GCAUC...UUG-3'\n______________GGCGAA_GCGCGUU\n______________::\|:\|\|_\|\|\|\|\|\|:\n______________UUGUUU_CGCGCAG\n3'-UUU...UACUA______A_______AAAG-5'\n_____________\|______________\|\n_____________19_____________4\n	285 -- 291	5 -- 11	-16.0784 kcal/mol	5.14159 kcal/mol	1.2243 kcal/mol	278 292@LB@ \| \|@LB@5'-UUG...AAAAA GCAUC...UUG-3'@LB@ GGCGAA GCGCGUU@LB@ ::\|:\|\| \|\|\|\|\|\|:@LB@ UUGUUU CGCGCAG@LB@3'-UUU...UACUA A AAAG-5'@LB@ \| \|@LB@ 19 4@LB@@LB@@LB@Energy -9.71 kcal/mol@LB@Hybridization Energy -16.0784 kcal/mol@LB@Unfolding Energy - mRNA 5.14159 kcal/mol@LB@Unfolding Energy - sRNA 1.2243 kcal/mol@LB@@LB@Position - mRNA 279 -- 291@LB@Position - sRNA 5 -- 18@LB@Position Seed - mRNA 285 -- 291@LB@Position Seed - sRNA 5 -- 11@LB@
72	0.006739	0.3087	b2520	yfhM	-8.19	0.237735	190 -- 217	6 -- 30	bacterial alpha2-macroglobulin colonization factor ECAM; anti-host protease defense factor; periplasmic inner membrane-anchored lipoprotein	_____________189__________________________218\n_____________\|____________________________\|\n5'-CUC...GUGGC________AAA____AA____UA_____AGCCG...CUU-3'\n______________AGGGAUGA___AUGA__AAGU__CGCGU\n______________\|\|\|\|\|\|\|\|___\|\|:\|__\|\|:\|__\|\|\|\|\|\n______________UCCCUACU___UAUU__UUUA__GCGCA\n3'-UUU...UUAAG________AC_____G_____C______GAAAG-5'\n_____________\|____________________________\|\n_____________31___________________________5\n	191 -- 197	23 -- 29	-19.496 kcal/mol	3.87755 kcal/mol	7.4255 kcal/mol	189 218@LB@ \| \|@LB@5'-CUC...GUGGC AAA AA UA AGCCG...CUU-3'@LB@ AGGGAUGA AUGA AAGU CGCGU@LB@ \|\|\|\|\|\|\|\| \|\|:\| \|\|:\| \|\|\|\|\|@LB@ UCCCUACU UAUU UUUA GCGCA@LB@3'-UUU...UUAAG AC G C GAAAG-5'@LB@ \| \|@LB@ 31 5@LB@@LB@@LB@Energy -8.19 kcal/mol@LB@Hybridization Energy -19.496 kcal/mol@LB@Unfolding Energy - mRNA 3.87755 kcal/mol@LB@Unfolding Energy - sRNA 7.4255 kcal/mol@LB@@LB@Position - mRNA 190 -- 217@LB@Position - sRNA 6 -- 30@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 23 -- 29@LB@
89	0.009028	0.3104	b0529	folD	-8.62	0.203566	239 -- 251	4 -- 17	bifunctional 5 10-methylene-tetrahydrofolate dehydrogenase/ 5 10-methylene-tetrahydrofolate cyclohydrolase	_____________238____________252\n_____________\|______________\|\n5'-CAA...GCGCA______________GAAGU...CAC-3'\n______________GCAGGUGCGC_UCU\n______________:\|\|::\|\|\|\|\|_\|\|\|\n______________UGUUUACGCG_AGA\n3'-UUU...ACUAU__________C___AAG-5'\n_____________\|______________\|\n_____________18_____________3\n	242 -- 248	8 -- 14	-17.587 kcal/mol	7.73476 kcal/mol	1.22888 kcal/mol	238 252@LB@ \| \|@LB@5'-CAA...GCGCA GAAGU...CAC-3'@LB@ GCAGGUGCGC UCU@LB@ :\|\|::\|\|\|\|\| \|\|\|@LB@ UGUUUACGCG AGA@LB@3'-UUU...ACUAU C AAG-5'@LB@ \| \|@LB@ 18 3@LB@@LB@@LB@Energy -8.62 kcal/mol@LB@Hybridization Energy -17.587 kcal/mol@LB@Unfolding Energy - mRNA 7.73476 kcal/mol@LB@Unfolding Energy - sRNA 1.22888 kcal/mol@LB@@LB@Position - mRNA 239 -- 251@LB@Position - sRNA 4 -- 17@LB@Position Seed - mRNA 242 -- 248@LB@Position Seed - sRNA 8 -- 14@LB@
125	0.01241	0.3104	b0002	thrA	-8.21	0.235915	191 -- 205	9 -- 21	Bifunctional aspartokinase/homoserine dehydrogenase 1	_____________190_____________206\n_____________\|_______________\|\n5'-CCA...AACGA________CC_____AGUGU...UGG-3'\n______________GGUAACAA__AUGCG\n______________\|:\|\|\|\|\|\|__\|\|\|\|\|\n______________CUAUUGUU__UACGC\n3'-UUU...UACUA_______________GCAGAAAG-5'\n_____________\|_______________\|\n_____________22______________8\n	192 -- 198	14 -- 20	-12.9357 kcal/mol	3.48482 kcal/mol	1.23627 kcal/mol	190 206@LB@ \| \|@LB@5'-CCA...AACGA CC AGUGU...UGG-3'@LB@ GGUAACAA AUGCG@LB@ \|:\|\|\|\|\|\| \|\|\|\|\|@LB@ CUAUUGUU UACGC@LB@3'-UUU...UACUA GCAGAAAG-5'@LB@ \| \|@LB@ 22 8@LB@@LB@@LB@Energy -8.21 kcal/mol@LB@Hybridization Energy -12.9357 kcal/mol@LB@Unfolding Energy - mRNA 3.48482 kcal/mol@LB@Unfolding Energy - sRNA 1.23627 kcal/mol@LB@@LB@Position - mRNA 191 -- 205@LB@Position - sRNA 9 -- 21@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 14 -- 20@LB@
170	0.01687	0.3104	b0033	carB	-11.35	0.071227	1 -- 15	5 -- 17	carbamoyl-phosphate synthase large subunit	______________1______________16\n______________\|______________\|\n___________5'-_____CC________ACGCA...CGU-3'\n______________GCAAA__UGCGUGUC\n______________:\|\|\|\|__\|\|\|\|:\|\|\|\n______________UGUUU__ACGCGCAG\n3'-UUU...ACUAU_______________AAAG-5'\n_____________\|_______________\|\n_____________18______________4\n	9 -- 15	5 -- 11	-15.984 kcal/mol	3.41554 kcal/mol	1.22241 kcal/mol	1 16@LB@ \| \|@LB@ 5'- CC ACGCA...CGU-3'@LB@ GCAAA UGCGUGUC@LB@ :\|\|\|\| \|\|\|\|:\|\|\|@LB@ UGUUU ACGCGCAG@LB@3'-UUU...ACUAU AAAG-5'@LB@ \| \|@LB@ 18 4@LB@@LB@@LB@Energy -11.35 kcal/mol@LB@Hybridization Energy -15.984 kcal/mol@LB@Unfolding Energy - mRNA 3.41554 kcal/mol@LB@Unfolding Energy - sRNA 1.22241 kcal/mol@LB@@LB@Position - mRNA 1 -- 15@LB@Position - sRNA 5 -- 17@LB@Position Seed - mRNA 9 -- 15@LB@Position Seed - sRNA 5 -- 11@LB@
168	0.01645	0.3104	b3720	bglH	-8.64	0.202347	15 -- 28	9 -- 22	carbohydrate-specific outer membrane porin cryptic	_____________14_____________29\n_____________\|______________\|\n5'-AUU...GAUCG_________UG___AAGGA...GCC-3'\n______________UGAUGAUAA__GCG\n______________\|\|\|\|:\|:\|\|__\|\|\|\n______________ACUAUUGUU__CGC\n3'-UUU...CUACU_________UA___GCAGAAAG-5'\n_____________\|______________\|\n_____________23_____________8\n	17 -- 23	14 -- 20	-13.7208 kcal/mol	3.8428 kcal/mol	1.23827 kcal/mol	14 29@LB@ \| \|@LB@5'-AUU...GAUCG UG AAGGA...GCC-3'@LB@ UGAUGAUAA GCG@LB@ \|\|\|\|:\|:\|\| \|\|\|@LB@ ACUAUUGUU CGC@LB@3'-UUU...CUACU UA GCAGAAAG-5'@LB@ \| \|@LB@ 23 8@LB@@LB@@LB@Energy -8.64 kcal/mol@LB@Hybridization Energy -13.7208 kcal/mol@LB@Unfolding Energy - mRNA 3.8428 kcal/mol@LB@Unfolding Energy - sRNA 1.23827 kcal/mol@LB@@LB@Position - mRNA 15 -- 28@LB@Position - sRNA 9 -- 22@LB@Position Seed - mRNA 17 -- 23@LB@Position Seed - sRNA 14 -- 20@LB@
9	0.000195	0.07335	b1830	prc	-15.09	0.015143	131 -- 161	7 -- 38	carboxy-terminal protease for penicillin-binding protein 3	_____________130______________________________162\n_____________\|________________________________\|\n5'-CGA...UCCAG___________U___UCUU____U________CAGAA...CGG-3'\n______________CUGAAUUCGGG_AUG____UGAU__GUGCGCG\n______________\|\|\|\|\|\|\|\|:\|\|_\|\|\|____\|:\|:__:\|\|\|\|\|\|\n______________GACUUAAGUCC_UAC____AUUG__UACGCGC\n3'-UUU...GUAGA___________C___UACU____UU_______AGAAAG-5'\n_____________\|________________________________\|\n_____________39_______________________________6\n	155 -- 161	7 -- 13	-31.7028 kcal/mol	7.25259 kcal/mol	9.35859 kcal/mol	130 162@LB@ \| \|@LB@5'-CGA...UCCAG U UCUU U CAGAA...CGG-3'@LB@ CUGAAUUCGGG AUG UGAU GUGCGCG@LB@ \|\|\|\|\|\|\|\|:\|\| \|\|\| \|:\|: :\|\|\|\|\|\|@LB@ GACUUAAGUCC UAC AUUG UACGCGC@LB@3'-UUU...GUAGA C UACU UU AGAAAG-5'@LB@ \| \|@LB@ 39 6@LB@@LB@@LB@Energy -15.09 kcal/mol@LB@Hybridization Energy -31.7028 kcal/mol@LB@Unfolding Energy - mRNA 7.25259 kcal/mol@LB@Unfolding Energy - sRNA 9.35859 kcal/mol@LB@@LB@Position - mRNA 131 -- 161@LB@Position - sRNA 7 -- 38@LB@Position Seed - mRNA 155 -- 161@LB@Position Seed - sRNA 7 -- 13@LB@
116	0.01184	0.3104	b1218	chaC	-12.45	0.045440	10 -- 29	8 -- 30	cation transport regulator	_____________9_______________________30\n_____________\|_______________________\|\n5'-AUA...GCCAA_______________________AUAAA...CGC-3'\n______________AGGG__GAUGAUGAUAAAUG_GC\n______________\|\|\|\|__\|\|\|\|\|\|:\|:\|\|\|\|\|_\|\|\n______________UCCC__CUACUAUUGUUUAC_CG\n3'-UUU...UUAAG____UA______________G__CAGAAAG-5'\n_____________\|_______________________\|\n_____________31______________________7\n	21 -- 27	11 -- 17	-25.0616 kcal/mol	5.19055 kcal/mol	7.41684 kcal/mol	9 30@LB@ \| \|@LB@5'-AUA...GCCAA AUAAA...CGC-3'@LB@ AGGG GAUGAUGAUAAAUG GC@LB@ \|\|\|\| \|\|\|\|\|\|:\|:\|\|\|\|\| \|\|@LB@ UCCC CUACUAUUGUUUAC CG@LB@3'-UUU...UUAAG UA G CAGAAAG-5'@LB@ \| \|@LB@ 31 7@LB@@LB@@LB@Energy -12.45 kcal/mol@LB@Hybridization Energy -25.0616 kcal/mol@LB@Unfolding Energy - mRNA 5.19055 kcal/mol@LB@Unfolding Energy - sRNA 7.41684 kcal/mol@LB@@LB@Position - mRNA 10 -- 29@LB@Position - sRNA 8 -- 30@LB@Position Seed - mRNA 21 -- 27@LB@Position Seed - sRNA 11 -- 17@LB@
4	4.059e-05	0.03436	b2748	ftsB	-19.92	0.001892	154 -- 190	1 -- 44	cell division protein	_____________153___________________________________________191\n_____________\|_____________________________________________\|\n5'-GGG...UAGAG_______C____G__U_____________________________AGGGG...AUG-3'\n______________UCGUCUU_GGAU_CA_GGGAUGAUGA_______UGC_CGUUUUUC\n______________\|\|:\|\|\|:_\|:\|\|_\|\|_\|\|\|\|\|\|\|\|\|\|_______\|\|\|_\|\|\|:\|\|\|\|\n______________AGUAGAG_CUUA_GU_CCCUACUACU_______ACG_GCAGAAAG\n3'-UUU...UUUAA_______A____A_____________AUUGUUU___C________-5'\n_____________\|____________________________________________\|\n_____________45___________________________________________1\n	184 -- 190	1 -- 7	-40.1368 kcal/mol	10.7611 kcal/mol	9.45578 kcal/mol	153 191@LB@ \| \|@LB@5'-GGG...UAGAG C G U AGGGG...AUG-3'@LB@ UCGUCUU GGAU CA GGGAUGAUGA UGC CGUUUUUC@LB@ \|\|:\|\|\|: \|:\|\| \|\| \|\|\|\|\|\|\|\|\|\| \|\|\| \|\|\|:\|\|\|\|@LB@ AGUAGAG CUUA GU CCCUACUACU ACG GCAGAAAG@LB@3'-UUU...UUUAA A A AUUGUUU C -5'@LB@ \| \|@LB@ 45 1@LB@@LB@@LB@Energy -19.92 kcal/mol@LB@Hybridization Energy -40.1368 kcal/mol@LB@Unfolding Energy - mRNA 10.7611 kcal/mol@LB@Unfolding Energy - sRNA 9.45578 kcal/mol@LB@@LB@Position - mRNA 154 -- 190@LB@Position - sRNA 1 -- 44@LB@Position Seed - mRNA 184 -- 190@LB@Position Seed - sRNA 1 -- 7@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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