Input and runtime details for job 4935127 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_003197
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 4935127 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:12:23 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:14:40 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:16:11 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:17:25 CET 2020
	Job Completed		@ Mon Jan 27 14:17:35 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=4935127&toolName=IntaRNA ( 30 days expiry )

Description of the job

GcvB - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
STM1856	-40	-18	-39	-33	1.92	GcvB	64	87	80	86	7.14	-17.01	-26.07	-1.64922	AACACAACAUAUAACAUUAUAAC&GUUGUGAUGUUGUGUUGUUGUGUU	(((((((((((((((((((((((&))))))))))))))).))))))))	AUAUCUGUAUGGCUGCUUUUAAGCAACAAUACUCUAACACAACAUAUAACAUUAUAACUUACAAUAGGUUAACAAAUGGAAUUACAGCUUAUGCUUAACCACUUUUUCGAGCGCGUCAGAAAGGAUGCAAAUUUCAACGCAUUUCUAAUC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0024848	0.659913		1253375	similar to E. coli orf hypothetical protein (AAC74245.1) Blastp hit to AAC74245.1 (134 aa) 40% identity in aa 1 - 127
STM1498	-54	-38	-48	-42	4.66	GcvB	76	92	80	86	3.94	-16.29	-24.89	-1.57942	UUGCGAACACAACGACA&UGUUGUUGUGUUUGCAA	(((((((((((((((((&)))))))))))))))))	ACGCGCCCGGAGCUCCCCGUUUUGCGAACACAACGACAUUUAAAACUCUUUUUUGGAUGAGCAUAGUGAGCUGUUAUGAAAAUCACCAACCCUGAGGCAUUAAUGGCGGCCAGUCUCAGCCGACGAAGCCUGGUGAAAACCUCCGCUAUC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0041011	0.6975035		1253016	dimethyl sulfoxide reductase subunit A
STM3523	7	24	10	16	5.8	GcvB	72	89	80	86	4.11	-15.15	-25.06	-1.46889	CAAACACAACGGCAUGAC&GUUGUGUUGUUGUGUUUG	((((((((((((((((((&))))))))))))))))))	UCGAGAUAUUUUUCCAGGCCGGGUAGGGCGAAUGCCGCUAUCCGGCGCAAGGUUGGUUACUUUCAGAGAUUAUUCAUGAAACAAACACAACGGCAUGACGCGAUCAUUGAACUGGUAAAAAAACAGGGGUACGUCAGUACGGAAGAGCUG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0086358	0.7799855	glpR	1255046	DeoR/GlpR family transcriptional regulator
STM1576	4	30	4	10	5.35	GcvB	61	86	80	86	7.74	-14.27	-27.36	-1.38356	ACACGACAAAACGAGAAUUAUAACCGU&ACGGUUGUGAUGUUGUGUUGUUGUGU	(((((((((.((((..(((((((((((&))))))))))).)))).)))))))))	UUCUCAAAAGAGCGCGUGACGCUCUGUCUCUUUUUUGUGUUUUUCUUCAAAUGAUAUGCGCAUGUGAGGGGUAAAAUGACACGACAAAACGAGAAUUAUAACCGUUAUCUCUUGAGUGACUGGCGACCCGAAAAUCCAGCCUUCUGGGAG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0147888	0.7904009	narU	1253094	nitrate/nitrite transporter NarU
STM4312	-24	24	-20	-14	11.29	GcvB	53	90	80	86	9.63	-13.95	-34.87	-1.35254	GCUCACGCAACAAUUAAGCUGACUAUGGAUACACAACCGUCCGCCGAA&UUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGC	((..(((((((((...(((.........((.(((((((((..((((((&))))))..))))))))))))))...)))))))))..))	GCGAUAAUAUCACUAACAUCAAUACACCGACCUUUAUCGGGUCGCUUAGAAGCUCACGCAACAAUUAAGCUGACUAUGGAUACACAACCGUCCGCCGAACCAACGUUGGGAUCGACUGUUAGCAGUCUGGAUAACACCGUCACCCUUAAG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0178517	0.8311163		1255838
STM3344	-70	-27	-61	-55	11.46	GcvB	48	102	83	89	15.57	-18.35	-45.38	-1.77915	CGCGGGCAACGAGCACAACCACGCGGCACAGCAACCGCAAGUUC&GGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCG	(((((((..((((((((((.((((((((((.((((((.((((((&))))))........)))))))).)))))))).))))))))))......)))))))	GUUUACGCGGGCAACGAGCACAACCACGCGGCACAGCAACCGCAAGUUCUUGACAUCUAAUCGGGAUUAUAGGCAAUGGCUGAAAAUCAAUACUACGGCACUGGUCGCCGCAAAAGUUCCGCAGCUCGCGUGUUCAUCAAACCGGGCAAC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0009093	0.4343002	rpsI	1254867	30S ribosomal protein S9
STM0022	-74	-55	-63	-57	2.39	GcvB	127	147	129	135	5.89	-14.36	-22.64	-1.39229	UGUUGGGAGGCGAGAAAGGG&CCCUUUUUCACUUCCUGUACA	(((..(((((.(((((((((&))))))))).)))))...)))	CUGUUGGGAGGCGAGAAAGGGGCGUUACGCGCGUCACUGCGCGUAACAAACCGACGGCAUCAGGAUAUUACGCAGAUGAAAAAAAACGUACCGAUUUUCCUUCGAUUAUUGCUACUGCUGUCGGCGGCCGGCCUGUCGUUUGCCGCGCAG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0140165	0.7904009	bcfB	1251540	fimbrial chaperone protein
STM3083	-36	-6	-13	-7	4.19	GcvB	130	160	131	137	6.26	-15.04	-25.49	-1.45822	CAGAAUUGGUAAACAAAACAGGAGUGGGAAA&UUUUUCACUUCCUGUACAUUUACCCUGUCUG	((((...((((((....((((((((((((((&)))))))).))))))...))))))...))))	AGUGUGAUCAUAUUCACGGAUUUUGGUAUGGUGUUUAAGCAGAAUUGGUAAACAAAACAGGAGUGGGAAAAAUUCAUGGACACCAUAGCUCGCCAGUUAGACGCCGCAAGGCACCAAUUCAAAACGACUUAUUCACGUCAGGGAAUGGAA	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.009252	0.7883581		1254606	fructuronate reductase
STM1033	42	70	60	66	5.67	GcvB	132	162	137	143	7.38	-15.02	-28.07	-1.45628	GACAGCAGGGAACGGACGCGGGGAGGGAA&UUUCACUUCCUGUACAUUUACCCUGUCUGUC	((((((((((........(((((((((((&)))).)))))))........))))).)))))	ACGAAAACCGUAAGCUGGGACUGGUAUUUGAUACUGAUCCGGCACAUGACCCGGGGGAACAGGAUAAUGCCGGAAGUGAAGACAACAGUGGCGGCGAUAAAAAUGCCGCCGGUGAUGACAGCAGGGAACGGACGCGGGGAGGGAAGCAGU	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0093682	0.7883581		1252551	Gifsy-2 prophage Clp protease-like protein
STM0532	-28	7	-7	-1	2.42	GcvB	131	164	138	144	7.56	-14.18	-24.16	-1.37484	UGGACAACAAAUAGUAAAAGGAUAGGGAAUGAAAA&UUUUCACUUCCUGUACAUUUACCCUGUCUGUCCA	(((((((((....(((((...(((((((.((((((&)))))).)))))))...)))))..))).))))))	GCGCUGGGGCACACCUCUGGUGCGGAUACGCUGCUGGGGUUCUGGCUUGGACAACAAAUAGUAAAAGGAUAGGGAAUGAAAACGUUGGUAGUUGCUCUUGGCGGCAACGCAUUAUUGCAGCGCGGAGAAGCGCUAACGGCAGAGAAUCAA	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0155989	0.7979576	arcC	1252052	putative carbamate kinase

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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