Input and runtime details for job 4935127 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_003197
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 4935127 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:12:23 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:14:40 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:16:11 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:17:25 CET 2020
	Job Completed		@ Mon Jan 27 14:17:35 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=4935127&toolName=IntaRNA ( 30 days expiry )

Description of the job

GcvB - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
STM1107	4	61	6	12	17.59	GcvB	36	82	74	80	10.87	-20.55	-49.01	-1.99245	AGCGACACAUCAUCUGCACUUCCGGAAAGCCCCGAGUCUGUCAGUUCGCACAACGCGC&GCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUU	(((((((((.((((...((..(((..(((((...((((((((((.......(((((((&)))))))))))))))))...))))).)))..)))))).)))))))))	UUAAGCCUGGCCCCGUAAGCGCAGCGCUACCGGGCAACCGUAGUACCCUACAAAAUUCCCAUCAGAGGAAAAAAAAUGAGCGACACAUCAUCUGCACUUCCGGAAAGCCCCGAGUCUGUCAGUUCGCACAACGCGCUCAGCACGGGUCAA	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0001353	0.2036716	hpaX	1252625	4-hydroxyphenylacetate permease
STM1564	19	40	32	38	3.82	GcvB	72	90	74	80	4.11	-16.06	-23.99	-1.55712	GCAAAUACCUCAGCAGCACAAC&GUUGUGUUGUUGUGUUUGC	((((((((...(((((((((((&)))))))))))))))))))	AAUAUUUGCCAUGCUGAAUACGAAUUAAUUUUCUGCGAGCCGUACUAAAUAAAUACAUGUUCACGGAGGCAUUAUAUGUUUACUUAUCACUCAGCAAAUACCUCAGCAGCACAACCGGCCCUGGUCAACGCGAUUGAACAAGGAUUACGC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.004788	0.6975035	yddX	1253082	biofilm-dependent modulation protein
STM1538	-52	-14	-43	-37	3.31	GcvB	51	90	75	81	10.1	-16.93	-30.34	-1.64147	GCAAUAAAAACAACACGUCAUCAGAAAACGCUCCGGAAG&CUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGC	((((....((((((((..(((((.((..((..(((((((&)))))))....)).)).)))))..))))))))....))))	GGUGAGCGCCAUAAAACAUGCCCGCAAUAAAAACAACACGUCAUCAGAAAACGCUCCGGAAGAGAAAAAAUAAUUAUGGCAUAUCCUUAUCAGACUCAGGGUUUUACGCUGGACAAUAGCGGACGGCGAAUUGUGGUCGAUCCGGUUACU	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0026303	0.659913		1253056	putative hydrogenase-1 large subunit
STM2355	-57	-39	-48	-42	3.35	GcvB	72	91	75	81	4.11	-16.13	-23.59	-1.5639	UGCAAACACACAACGCCAC&GUUGUGUUGUUGUGUUUGCA	((((((((((((((((.((&)).))))))).)))))))))	GACCUGCAUGAAAAAGUCUGCAAACACACAACGCCACGUAAAACAUAAGAAAAUGACGCCACUUGAGGGGUAUGUAUGAAGAAGACCGUUCUCGCUUUGUCUUUGCUGAUAGGUCUGGGCGCGACGGCGGCCAGUUACGCCGCGCUCCCG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0045687	0.6975035	argT	1253877	histidine ABC transporter substrate-binding protein HisJ
STM3567	-51	-28	-45	-39	4.12	GcvB	63	87	75	81	7.58	-15.35	-27.05	-1.48828	AACACCACAACACACGUAACAACC&GGUUGUGAUGUUGUGUUGUUGUGUU	(((((.(((((((((((.((((((&)))))).)))).))))))).)))))	AAUCGAAAUAAAGUGCUGAACAAUAACACCACAACACACGUAACAACCAGAAUAAUGGGGAUUAUCAGGAUGAAUAUGAAGGGUAAAACGUUAUUGGCAGGAUGUAUCGCCCUGUCAUUAAGCCAUAUGGCAUUCGCCGACGACAUUAAA	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0076089	0.7711365	livJ	1255090	high-affinity branched-chain amino acid transporter
STM0069	-71	-52	-71	-65	3.48	GcvB	60	81	75	81	6.88	-14.87	-25.23	-1.44174	ACAGCACAUGCGCAACCGUA&UACGGUUGUGAUGUUGUGUUGU	((((((((..((((((((((&))))))))))....))))))))	GCCUACAGCACAUGCGCAACCGUAGACCGGAUAAGGUGUUUACGCCGCCAUCCGGCAAAAAACCGAGGUACAACUAUGAGCUAUUACGCCUUUGAAGGACUGAUCCCGGUGGUGCAUCCGGACGCGUUUGUCCACCCCAGCGCCGUGCUG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0102821	0.7883581	caiE	1251587	phenylacetic acid degradation protein PaaY
STM3810	-30	-11	-21	-15	3.57	GcvB	72	91	77	83	4.11	-14.7	-22.38	-1.42526	UGCAAAUAACGACAACCAAC&GUUGUGUUGUUGUGUUUGCA	((((((((.(((((((((((&)))).)))))))))))))))	GUACUGGUGAUGUUAUUUUUAGCAACGGAUCGCUAUAACUCAUCAUGCAAAUAACGACAACCAACAGAUGAAUGAGUGAUGAUAAAAAACGUGUGGUUAACGCUGAUAAUAUGGAAUGGGAUGCUUUUAUUAAGCGGUUGUUCAAGCGUC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0114137	0.7904009	yidQ	1255337	putative outer membrane lipoprotein
STM2384	-30	-16	-22	-16	0.56	GcvB	77	90	77	83	3.93	-14.64	-19.13	-1.41944	GCAAACGACAACAAC&GUUGUUGUGUUUGC	((((((.((((((((&))))))))))))))	GCUCGCGGCCCGUGAACAUUUCAAUAUUUAUAAAGAUUAAAACACGCAAACGACAACAACGAUAACGGAGCCGUGAUGGCAGGAAACACAAUUGGACAACUCUUUCGCGUAACCACUUUCGGCGAAUCACACGGGCUGGCGCUUGGGUGU	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0118389	0.7904009	aroC	1253906	chorismate synthase
STM2526	-32	-17	-31	-25	0.58	GcvB	71	86	79	85	3.06	-14.36	-18	-1.39229	ACAUAACAACAGAACA&UGUUGUGUUGUUGUGU	(((((((((((.((((&)))).)))))))))))	AUCCAUAGGCAAUUGAUGGGAUGCUCUUUAUAAUCCGCGCCUGACAUAACAACAGAACAUAUUUCAGAGGUAAACAUGGCUAUUGAACGGACUUUUUCCAUCAUUAAACCCAACGCGGUGGCAAAAAACGUUAUUGGCAGCAUCUUUGCG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0140165	0.7904009	ndk	1254048	nucleoside-diphosphate kinase
STM0369	2	69	8	14	12.59	GcvB	31	91	80	86	17.22	-18.82	-48.63	-1.82471	UGACAGACACGACGAUCCUGCAAAACAACACGCAUGUCAUUAAGCCUAAAAAAUCGGUCGCGCUUUCC&GGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCA	((.((((((((((((.....((..(((((.((...((((..(((((((.....((((.((((..((((&))))..))))..))))))))))).))))...)))))))..)).....))))))))))))))	CACUACGUUUAACCCGCCUACGGUACGGCCUACACGCCUCAAUACCCUACACAUUACAAUAAUGACGAGGACAAUAUGACAGACACGACGAUCCUGCAAAACAACACGCAUGUCAUUAAGCCUAAAAAAUCGGUCGCGCUUUCCGGCGUA	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0006231	0.3847632	prpC	1251888	2-methylcitrate synthase

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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