Input and runtime details for job 4935127 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_003197
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 4935127 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:12:23 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:14:40 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:16:11 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:17:25 CET 2020
	Job Completed		@ Mon Jan 27 14:17:35 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=4935127&toolName=IntaRNA ( 30 days expiry )

Description of the job

GcvB - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
STM2909	41	74	68	74	7.52	GcvB	47	83	47	53	11.67	-16.31	-35.5	-1.58135	CGAUGAUGCAGCAGUAUCUCAAGCUGAAAGCCCA&UGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUG	(((((((((((((..(((..((((((((((((((&))))))))))))))..))).....)))))))))))))	UUUAUGUGAGUUUAUAAGGGUUUUACCUGGGGCCACAUGAGCACUUGAAAGAAUAAAAAAACGAGUAAAAUCAAUAUGAAUGAGUCAUUUGAUAAGGACUUCUCCAACCACACCCCGAUGAUGCAGCAGUAUCUCAAGCUGAAAGCCCAG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0040457	0.6975035	mutS	1254432	DNA mismatch repair protein MutS
STM2925	58	74	59	65	5.88	GcvB	153	170	163	169	2.49	-14.45	-22.82	-1.40102	UCACUAUGGCUGGCAGG&CCUGUCUGUCCAUAGUGA	(((((((((..((((((&))))))...)))))))))	UUUCCCGUGUUCACUUAAUGCGUUGCACGGUUCUUACGUCACUGGUUGUUAACCAAAUUUUUUCCUGGGGGAUAAAUGAGCGCGGGAAGCCCAAAAUUCACCGUUAGUCGCAUUGCGGCACUAUCACUGGUUUCACUAUGGCUGGCAGGU	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0132805	0.7904009	nlpD	1254448	lipoprotein NlpD
STM3021	29	67	43	49	10.69	GcvB	50	91	72	78	10.46	-14.5	-35.65	-1.40586	UGCAACAUGACGCCAUACAACGUCGCUCGCUGCCGGAGC&GCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCA	(((((((((((...((((((((((((.((..((((((((&)).))))))...))...)))))))))))).))))))).))))	ACCUAUCUACUUUAUCGAUGGAUGUUAUCUUUUAGGUAUCACAUUAAACGAUAAAAUAGCGGAAAUUUCUUUAGCUUGAUUAAGAGUAAGGUUUUUUCGUUUAUGCAACAUGACGCCAUACAACGUCGCUCGCUGCCGGAGCGUAUUUUC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0128866	0.7904009		1254544	putative inner membrane protein
STM3076	-22	13	-22	-16	5.91	GcvB	35	75	69	75	10.97	-14.34	-31.22	-1.39035	CAACAUCCGAUCUGGAGUCAAAAUGUCCUCACGUA&UGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUG	((((((((((((...((((((((.((((.((((((&)))))).......))))))))))))...))))).)))))))	UUCCGGCGUAGCCCAAAACGCGCUGUCGUCAAGUCGUUACGGGCAUGGCCUUUCAACAUCCGAUCUGGAGUCAAAAUGUCCUCACGUAAAGAGCUUGCCAAUGCUAUUCGUGCGCUGAGCAUGGACGCAGUACAGAAAGCCAAGUCCGGU	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0141849	0.7904009	tktA	1254599	transketolase
STM3083	-36	-6	-13	-7	4.19	GcvB	130	160	131	137	6.26	-15.04	-25.49	-1.45822	CAGAAUUGGUAAACAAAACAGGAGUGGGAAA&UUUUUCACUUCCUGUACAUUUACCCUGUCUG	((((...((((((....((((((((((((((&)))))))).))))))...))))))...))))	AGUGUGAUCAUAUUCACGGAUUUUGGUAUGGUGUUUAAGCAGAAUUGGUAAACAAAACAGGAGUGGGAAAAAUUCAUGGACACCAUAGCUCGCCAGUUAGACGCCGCAAGGCACCAAUUCAAAACGACUUAUUCACGUCAGGGAAUGGAA	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.009252	0.7883581		1254606	fructuronate reductase
STM3089	56	73	67	73	1.87	GcvB	157	174	157	163	3.11	-16.07	-21.05	-1.55809	UUAAUCAGUAUGGAUGGA&UCUGUCCAUAGUGAUUAA	(((((((.((((((((((&)))))))))).)))))))	GUAAAAAGGUGUUUUGCCAUAUUUAAUUUCACCUAAGGAGAAUUUAGUUAGCUCAAACUGUUGUGCGGAAUAGUCAUGACAGAAGAUUCUACCAUGAGUCUGAAGGUUGCUGACACUGGCAGUCUGAGUGUUAAUCAGUAUGGAUGGAUU	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0047561	0.6975035	yqgD	1254612	similar to E. coli orf hypothetical protein (AAC75978.1) Blastp hit to AAC75978.1 (83 aa) 52% identity in aa 1 - 78
STM3093	-66	-41	-47	-41	4.25	GcvB	72	101	72	78	11.77	-15.59	-31.61	-1.51155	GCGGACUGAUUGCAUACAGACACAAC&GUUGUGUUGUUGUGUUUGCAAUUGGUCUGC	((((((((((((((((((.(((((((&)))))))...)))))..)))))))))))))	CUUUCCUGAGCGGACUGAUUGCAUACAGACACAACUUUCGUUACGUUGCGGGCUCGUUUUGCUAUGGAGUGUGCAAUGUACCGUAAUUUUUCUUUUGCCGCUGCGUUGCUGGCCGCAGCGUUUUCAGGCCAGGCCCUGGCCGAUGGCAUU	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.006522	0.7419678	endA	1254616	deoxyribonuclease I
STM3236	25	40	34	40	5.36	GcvB	154	169	154	160	2.34	-15.54	-23.24	-1.5067	CAAUGUGGACAGGCAG&CUGUCUGUCCAUAGUG	((.(((((((((((((&))))))))))))).))	CCGGGCAGAAUAUCUGGCUAACAAUGCAGCGUCCAGCCCCUUAACAUAAAAGGAAGUAAAGAGAGGUUAAUAACGAUGAUUACGACCCGAACCGCCAAACAAUGUGGACAGGCAGACUACGGAUGGCUACAGGCCCGCUAUACCUUCUCC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0067362	0.7419678	yhaK	1254759	similar to E. coli orf hypothetical protein (AAC76141.1) Blastp hit to AAC76141.1 (233 aa) 84% identity in aa 1 - 233
STM3251	-5	39	5	11	7.81	GcvB	45	87	72	78	12.16	-20.16	-40.13	-1.95464	AACGCAUGAACAUAACAAUCGCAACAUUGGGUGAAUUGCUCGUC&GAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUU	((((((..(((((((((.(((((((..((((..((..(((((((&)))))))..))..))))..))))))))))))))))..))))))	CCAACAGCUAUACCAAUAACGAAUGUCUGACCUGGAUAAACCCGACCCUGCAUCAGGCAAGAUGAAGGACAACGCAUGAACAUAACAAUCGCAACAUUGGGUGAAUUGCUCGUCGAGUUUCUGGCAAAAAAAGAGAACCAGGGAUUUUCC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0001949	0.2200421		1254774	sugar kinase
STM3257	-68	-44	-63	-57	1.65	GcvB	46	81	70	76	9.96	-13.98	-25.59	-1.35545	ACAAAACAACAUUACGAAAGCCUAU&AUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGU	((((.((((((((((((((((((((&))))))))...........)))))))))))).))))	CAAAAUCACAAAACAACAUUACGAAAGCCUAUAAAUUCUUUCGAAGAAGAAAAACGAAAGAACUCGGAGAUAAAAGUGAAAGAAAUAAUUGCUCGUCAUAAAGCAGGAGAACAUCUGGGCAUCUGUUCAGUUUGUUCUGCUCAUCCCCUG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0175423	0.8300455		1254780	D-tagatose-16-bisphosphate aldolase subunit GatZ

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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