Input and runtime details for job 8257533 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 8257533 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:34:31 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:34:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:30:38 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:30:45 CET 2018
	Job Completed		@ Fri Feb 16 22:31:01 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=8257533&toolName=CopraRNA ( 30 days expiry )

Description of the job

LhrA2

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003210 Listeria monocytogenes EGD-e

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
91	0.02389	0.6733	lmo2425	N/A	-16.15	0.059838	240 -- 263	146 -- 170	glycine cleavage system protein H	_____________239______________264\n_____________\|________________\|\n5'-CGA...AACAC________________GACGA...AUC-3'\n______________GA_AUGGGUGAAAGCA\n______________\|\|_\|\|\|::\|\|\|\|\|:\|\|\n______________CU_UACUUACUUUUGU\n3'-GUU...GGUUA__C_____________UUAUU...GGA-5'\n_____________\|________________\|\n_____________171______________145\n	248 -- 254	155 -- 161	-15.1065 kcal/mol	3.08859 kcal/mol	0.929544 kcal/mol	239 264@LB@ \| \|@LB@5'-CGA...AACAC GACGA...AUC-3'@LB@ GA AUGGGUGAAAGCA@LB@ \|\| \|\|\|::\|\|\|\|\|:\|\|@LB@ CU UACUUACUUUUGU@LB@3'-GUU...GGUUA C UUAUU...GGA-5'@LB@ \| \|@LB@ 171 145@LB@@LB@@LB@Energy -16.15 kcal/mol@LB@Hybridization Energy -15.1065 kcal/mol@LB@Unfolding Energy - mRNA 3.08859 kcal/mol@LB@Unfolding Energy - sRNA 0.929544 kcal/mol@LB@@LB@Position - mRNA 240 -- 263@LB@Position - sRNA 146 -- 170@LB@Position Seed - mRNA 248 -- 254@LB@Position Seed - sRNA 155 -- 161@LB@
92	0.02404	0.6733	lmo1947	resE	-16.63	0.048416	181 -- 212	141 -- 170	two-component sensor histidine kinas	_____________180_________________213\n_____________\|___________________\|\n5'-AAG...ACCGA_______AUA_________UUGGA...UUU-3'\n______________GGUGAGA___GAUGAAGAU\n______________::\|\|\|\|\|___:\|\|\|\|\|:\|:\n______________UUACUCU___UUACUUUUG\n3'-GUU...GCCGG_______AC__________UUUAU...GGA-5'\n_____________\|___________________\|\n_____________171_________________140\n	202 -- 208	156 -- 162	-13.4823 kcal/mol	2.25638 kcal/mol	0.941471 kcal/mol	180 213@LB@ \| \|@LB@5'-AAG...ACCGA AUA UUGGA...UUU-3'@LB@ GGUGAGA GAUGAAGAU@LB@ ::\|\|\|\|\| :\|\|\|\|\|:\|:@LB@ UUACUCU UUACUUUUG@LB@3'-GUU...GCCGG AC UUUAU...GGA-5'@LB@ \| \|@LB@ 171 140@LB@@LB@@LB@Energy -16.63 kcal/mol@LB@Hybridization Energy -13.4823 kcal/mol@LB@Unfolding Energy - mRNA 2.25638 kcal/mol@LB@Unfolding Energy - sRNA 0.941471 kcal/mol@LB@@LB@Position - mRNA 181 -- 212@LB@Position - sRNA 141 -- 170@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 156 -- 162@LB@
93	0.02416	0.6733	lmo2042	N/A	-17.13	0.038629	149 -- 196	158 -- 206	cell division protein MraZ	_____________148_____________________________________________197\n_____________\|_______________________________________________\|\n5'-UUU...GCGAA_________________UAAAG_____AUGU____AA__________UCUAA...CCC-3'\n______________GUGGGG___AGAUGUGG______UGGU____AUGA__GUGGGUGAAG\n______________:\|\|:\|\|___\|\|\|::\|\|:______:\|\|:____\|\|\|\|__:\|\|::\|\|\|\|:\n______________UACUCC___UCUGUACU______GCCG____UACU__UACUUACUUU\n3'-GUU...UGCCC______GUA________UGAUGU____GU______C___________UGUUU...GGA-5'\n_____________\|_______________________________________________\|\n_____________207_____________________________________________157\n	190 -- 196	158 -- 164	-27.738 kcal/mol	5.20754 kcal/mol	13.9391 kcal/mol	148 197@LB@ \| \|@LB@5'-UUU...GCGAA UAAAG AUGU AA UCUAA...CCC-3'@LB@ GUGGGG AGAUGUGG UGGU AUGA GUGGGUGAAG@LB@ :\|\|:\|\| \|\|\|::\|\|: :\|\|: \|\|\|\| :\|\|::\|\|\|\|:@LB@ UACUCC UCUGUACU GCCG UACU UACUUACUUU@LB@3'-GUU...UGCCC GUA UGAUGU GU C UGUUU...GGA-5'@LB@ \| \|@LB@ 207 157@LB@@LB@@LB@Energy -17.13 kcal/mol@LB@Hybridization Energy -27.738 kcal/mol@LB@Unfolding Energy - mRNA 5.20754 kcal/mol@LB@Unfolding Energy - sRNA 13.9391 kcal/mol@LB@@LB@Position - mRNA 149 -- 196@LB@Position - sRNA 158 -- 206@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 158 -- 164@LB@
94	0.02439	0.6733	lmo0952	N/A	-17.21	0.037353	188 -- 223	166 -- 199	lmo0952	_____________187__________________________________224\n_____________\|____________________________________\|\n5'-AAC...AAACG________UU__GAU_____A___AA__________AGUAC...UAA-3'\n______________GAGGCAUA__CA___GAUUA_ACG__CUGGUGAGAU\n______________\|\|\|\|\|\|\|\|__\|\|___:\|:\|\|_\|\|\|__\|:::\|\|\|\|\|\|\n______________CUCCGUAU__GU___UUGAU_UGC__GGUUACUCUA\n3'-GUU...CCCUA________CU__AC______G___C___________CUUAC...GGA-5'\n_____________\|____________________________________\|\n_____________200__________________________________165\n	217 -- 223	166 -- 172	-30.0979 kcal/mol	6.14462 kcal/mol	13.4403 kcal/mol	187 224@LB@ \| \|@LB@5'-AAC...AAACG UU GAU A AA AGUAC...UAA-3'@LB@ GAGGCAUA CA GAUUA ACG CUGGUGAGAU@LB@ \|\|\|\|\|\|\|\| \|\| :\|:\|\| \|\|\| \|:::\|\|\|\|\|\|@LB@ CUCCGUAU GU UUGAU UGC GGUUACUCUA@LB@3'-GUU...CCCUA CU AC G C CUUAC...GGA-5'@LB@ \| \|@LB@ 200 165@LB@@LB@@LB@Energy -17.21 kcal/mol@LB@Hybridization Energy -30.0979 kcal/mol@LB@Unfolding Energy - mRNA 6.14462 kcal/mol@LB@Unfolding Energy - sRNA 13.4403 kcal/mol@LB@@LB@Position - mRNA 188 -- 223@LB@Position - sRNA 166 -- 199@LB@Position Seed - mRNA 217 -- 223@LB@Position Seed - sRNA 166 -- 172@LB@
95	0.02474	0.6733	lmo2446	N/A	-18.44	0.021161	178 -- 278	95 -- 188	glycosidase	_____________177____________________279\n_____________\|______________________\|\n5'-UGA...AGGAA___________GC_A_______GAAAA...ACG-3'\n______________AGUGAGAUGAA__G_AGAACGA\n______________\|:\|\|\|\|\|\|\|\|\|__\|_\|:\|\|\|:\|\n______________UUACUCUACUU__C_UUUUGUU\n3'-GUU...GCCGG___________A__________UAUUA...GGA-5'\n_____________\|______________________\|\n_____________189____________________94\n	210 -- 216	154 -- 160	-17.9823 kcal/mol	4.45349 kcal/mol	0.959215 kcal/mol	177 279@LB@ \| \|@LB@5'-UGA...AGGAA GC A GAAAA...ACG-3'@LB@ AGUGAGAUGAA G AGAACGA@LB@ \|:\|\|\|\|\|\|\|\|\| \| \|:\|\|\|:\|@LB@ UUACUCUACUU C UUUUGUU@LB@3'-GUU...GCCGG A UAUUA...GGA-5'@LB@ \| \|@LB@ 189 94@LB@@LB@@LB@Energy -18.44 kcal/mol@LB@Hybridization Energy -17.9823 kcal/mol@LB@Unfolding Energy - mRNA 4.45349 kcal/mol@LB@Unfolding Energy - sRNA 0.959215 kcal/mol@LB@@LB@Position - mRNA 178 -- 278@LB@Position - sRNA 95 -- 188@LB@Position Seed - mRNA 210 -- 216@LB@Position Seed - sRNA 154 -- 160@LB@
96	0.02498	0.6733	lmo1278	clpQ	-17.33	0.035430	185 -- 216	173 -- 203	ATP-dependent protease peptidase subunit	_____________184_____217\n_____________\|_______\|\n5'-AAA...UCAAC_______AAAAG...UGA-3'\n______________AUGACGG\n______________\|\|:\|\|\|\|\n______________UAUUGCC\n3'-GUU...AUACG_______CUACU...GGA-5'\n_____________\|_______\|\n_____________204_____172\n	238 -- 244	203 -- 209	-8.20287 kcal/mol	1.40847 kcal/mol	0.374301 kcal/mol	184 217@LB@ \| \|@LB@5'-AAA...UCAAC AAAAG...UGA-3'@LB@ AUGACGG@LB@ \|\|:\|\|\|\|@LB@ UAUUGCC@LB@3'-GUU...AUACG CUACU...GGA-5'@LB@ \| \|@LB@ 204 172@LB@@LB@@LB@Energy -17.33 kcal/mol@LB@Hybridization Energy -8.20287 kcal/mol@LB@Unfolding Energy - mRNA 1.40847 kcal/mol@LB@Unfolding Energy - sRNA 0.374301 kcal/mol@LB@@LB@Position - mRNA 185 -- 216@LB@Position - sRNA 173 -- 203@LB@Position Seed - mRNA 238 -- 244@LB@Position Seed - sRNA 203 -- 209@LB@
97	0.02539	0.6733	lmo2585	N/A	-13.14	0.201793	84 -- 125	164 -- 211		_____________83______126\n_____________\|_______\|\n5'-ACA...GAUGC_______AAGGG...AAG-3'\n______________GGCGGGA\n______________::\|\|\|\|\|\n______________UUGCCCU\n3'-GUU...ACGUA_______ACUCC...GGA-5'\n_____________\|_______\|\n_____________212_____163\n	178 -- 184	201 -- 207	-10.7547 kcal/mol	3.27081 kcal/mol	0.326294 kcal/mol	83 126@LB@ \| \|@LB@5'-ACA...GAUGC AAGGG...AAG-3'@LB@ GGCGGGA@LB@ ::\|\|\|\|\|@LB@ UUGCCCU@LB@3'-GUU...ACGUA ACUCC...GGA-5'@LB@ \| \|@LB@ 212 163@LB@@LB@@LB@Energy -13.14 kcal/mol@LB@Hybridization Energy -10.7547 kcal/mol@LB@Unfolding Energy - mRNA 3.27081 kcal/mol@LB@Unfolding Energy - sRNA 0.326294 kcal/mol@LB@@LB@Position - mRNA 84 -- 125@LB@Position - sRNA 164 -- 211@LB@Position Seed - mRNA 178 -- 184@LB@Position Seed - sRNA 201 -- 207@LB@
98	0.02549	0.6733	lmo2155	N/A	-16.07	0.061955	240 -- 298	157 -- 207	ribonucleotide-diphosphate reductase subunit alpha	_____________239________299\n_____________\|__________\|\n5'-UAU...UAAAA__________AACAG...AAA-3'\n______________GACGGGG_GA\n______________:\|\|\|\|\|:_\|\|\n______________UUGCCCU_CU\n3'-GUU...ACGUA_______A__CCGUA...GGA-5'\n_____________\|__________\|\n_____________208________156\n	240 -- 246	201 -- 207	-11.5149 kcal/mol	1.00175 kcal/mol	0.374399 kcal/mol	239 299@LB@ \| \|@LB@5'-UAU...UAAAA AACAG...AAA-3'@LB@ GACGGGG GA@LB@ :\|\|\|\|\|: \|\|@LB@ UUGCCCU CU@LB@3'-GUU...ACGUA A CCGUA...GGA-5'@LB@ \| \|@LB@ 208 156@LB@@LB@@LB@Energy -16.07 kcal/mol@LB@Hybridization Energy -11.5149 kcal/mol@LB@Unfolding Energy - mRNA 1.00175 kcal/mol@LB@Unfolding Energy - sRNA 0.374399 kcal/mol@LB@@LB@Position - mRNA 240 -- 298@LB@Position - sRNA 157 -- 207@LB@Position Seed - mRNA 240 -- 246@LB@Position Seed - sRNA 201 -- 207@LB@
99	0.02562	0.6733	lmo2201	N/A	-18.35	0.022079	190 -- 255	101 -- 170	3-oxoacyl-ACP synthase	_____________189_____________________________________________________________________256\n_____________\|_______________________________________________________________________\|\n5'-AAC...AUAAG________________________GAAGA__A___GUU__U______G__GCU___A___C__________ACGAU...AUG-3'\n______________GAGAUGAGU__AAAUGGAUAA_GA_____GU_GUU___AC_GGUGUU_GA___GUU__CA__CUAUUGGAA\n______________\|\|\|\|\|\|\|:\|__\|\|\|:::\|\|\|\|_\|\|_____\|\|_\|\|:___\|\|_\|:\|\|\|:_\|\|___\|\|\|__\|\|__\|\|\|\|\|\|\|\|\|\n______________CUCUACUUA__UUUGUUUAUU_CU_____CA_CAG___UG_CUACAG_CU___CAA__GU__GAUAACCUU\n3'-GUU...GGUUA_________CU__________A__AAAAC______AUU__U______A__GAU___CG__AA_________CUACC...GGA-5'\n_____________\|_______________________________________________________________________\|\n_____________171_____________________________________________________________________100\n	249 -- 255	101 -- 107	-41.8612 kcal/mol	11.5076 kcal/mol	19.339 kcal/mol	189 256@LB@ \| \|@LB@5'-AAC...AUAAG GAAGA A GUU U G GCU A C ACGAU...AUG-3'@LB@ GAGAUGAGU AAAUGGAUAA GA GU GUU AC GGUGUU GA GUU CA CUAUUGGAA@LB@ \|\|\|\|\|\|\|:\| \|\|\|:::\|\|\|\| \|\| \|\| \|\|: \|\| \|:\|\|\|: \|\| \|\|\| \|\| \|\|\|\|\|\|\|\|\|@LB@ CUCUACUUA UUUGUUUAUU CU CA CAG UG CUACAG CU CAA GU GAUAACCUU@LB@3'-GUU...GGUUA CU A AAAAC AUU U A GAU CG AA CUACC...GGA-5'@LB@ \| \|@LB@ 171 100@LB@@LB@@LB@Energy -18.35 kcal/mol@LB@Hybridization Energy -41.8612 kcal/mol@LB@Unfolding Energy - mRNA 11.5076 kcal/mol@LB@Unfolding Energy - sRNA 19.339 kcal/mol@LB@@LB@Position - mRNA 190 -- 255@LB@Position - sRNA 101 -- 170@LB@Position Seed - mRNA 249 -- 255@LB@Position Seed - sRNA 101 -- 107@LB@
100	0.02596	0.6733	lmo2805	N/A	-13.33	0.188364	273 -- 298	147 -- 172	hypothetical secreted protein	_____________272_______________________________________299\n_____________\|_________________________________________\|\n5'-GAA...CAUAC______GG_____AG_____AAAAA________________AAUAG...AAA-3'\n______________AACAGA____UGA__AGUUG____________GAGAUGGAU\n______________\|\|\|\|\|\|____\|\|\|__\|\|\|\|\|____________:\|\|\|\|\|\|\|\|\n______________UUGUCU____ACU__UCAAC____________UUCUACCUA\n3'-GUU...CAGAU______ACAG___GA_____GGUAAGAUAACC_________UGUGG...GGA-5'\n_____________\|_________________________________________\|\n_____________173_______________________________________146\n	211 -- 217	94 -- 100	-22.9496 kcal/mol	2.56346 kcal/mol	9.49359 kcal/mol	272 299@LB@ \| \|@LB@5'-GAA...CAUAC GG AG AAAAA AAUAG...AAA-3'@LB@ AACAGA UGA AGUUG GAGAUGGAU@LB@ \|\|\|\|\|\| \|\|\| \|\|\|\|\| :\|\|\|\|\|\|\|\|@LB@ UUGUCU ACU UCAAC UUCUACCUA@LB@3'-GUU...CAGAU ACAG GA GGUAAGAUAACC UGUGG...GGA-5'@LB@ \| \|@LB@ 173 146@LB@@LB@@LB@Energy -13.33 kcal/mol@LB@Hybridization Energy -22.9496 kcal/mol@LB@Unfolding Energy - mRNA 2.56346 kcal/mol@LB@Unfolding Energy - sRNA 9.49359 kcal/mol@LB@@LB@Position - mRNA 273 -- 298@LB@Position - sRNA 147 -- 172@LB@Position Seed - mRNA 211 -- 217@LB@Position Seed - sRNA 94 -- 100@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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