Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
51	0.001671	0.1153	b3527	yhjJ	-13.34	0.053290	153 -- 204	31 -- 75	putative periplasmic M16 family chaperone		_____________152_____________________________________________________205\n_____________\|_______________________________________________________\|\n5'-CUC...UGACC___A___G_____UU___UAUUACCAGGAUA___GA____________CA_____AGGGC...GGC-3'\n______________GAC_ACG_GCAGU__UUU_____________GUU____UCAGGGG_UU__CAUGC\n______________\|\|\|_\|\|:_:\|\|\|:__\|\|:_____________\|\|\|____\|\|\|\|\|\|\|_\|\|__\|\|\|\|\|\n______________CUG_UGU_UGUCG__AAG_____________CAA____AGUCCCC_AA__GUACG\n3'-UUU...CGUUU___A___G_____UU___CAA_____________AGUG_______U__AG_____AAUAU...GCA-5'\n_____________\|_______________________________________________________\|\n_____________76______________________________________________________30\n	189 -- 195	41 -- 47	-32.6504 kcal/mol	12.0549 kcal/mol	7.26183 kcal/mol	152 205@LB@ \| \|@LB@5'-CUC...UGACC A G UU UAUUACCAGGAUA GA CA AGGGC...GGC-3'@LB@ GAC ACG GCAGU UUU GUU UCAGGGG UU CAUGC@LB@ \|\|\| \|\|: :\|\|\|: \|\|: \|\|\| \|\|\|\|\|\|\| \|\| \|\|\|\|\|@LB@ CUG UGU UGUCG AAG CAA AGUCCCC AA GUACG@LB@3'-UUU...CGUUU A G UU CAA AGUG U AG AAUAU...GCA-5'@LB@ \| \|@LB@ 76 30@LB@@LB@@LB@Energy -13.34 kcal/mol@LB@Hybridization Energy -32.6504 kcal/mol@LB@Unfolding Energy - mRNA 12.0549 kcal/mol@LB@Unfolding Energy - sRNA 7.26183 kcal/mol@LB@@LB@Position - mRNA 153 -- 204@LB@Position - sRNA 31 -- 75@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 41 -- 47@LB@
52	0.001789	0.121	b1799	dmlR	-11.56	0.127346	72 -- 108	34 -- 68	transcriptional activator of dmlA	b3060	_____________71_______________________________________109\n_____________\|________________________________________\|\n5'-CGC...GGGAU_________ACGCA_______________UU_____C___CUUCC...CGG-3'\n______________CGCAGCAAU_____UCGU__UUUCA_UCA__GGGUU_UCA\n______________\|:\|\|\|\|\|\|\|_____\|\|\|\|__\|\|\|\|\|_\|\|\|__\|\|:\|\|_:\|\|\n______________GUGUCGUUA_____AGCA__AAAGU_AGU__CCUAA_GGU\n3'-UUU...GAUGU__________________AC_____G___CC_____A___ACGAA...GCA-5'\n_____________\|________________________________________\|\n_____________69_______________________________________33\n	74 -- 80	60 -- 66	-24.8584 kcal/mol	8.52016 kcal/mol	4.78423 kcal/mol	71 109@LB@ \| \|@LB@5'-CGC...GGGAU ACGCA UU C CUUCC...CGG-3'@LB@ CGCAGCAAU UCGU UUUCA UCA GGGUU UCA@LB@ \|:\|\|\|\|\|\|\| \|\|\|\| \|\|\|\|\| \|\|\| \|\|:\|\| :\|\|@LB@ GUGUCGUUA AGCA AAAGU AGU CCUAA GGU@LB@3'-UUU...GAUGU AC G CC A ACGAA...GCA-5'@LB@ \| \|@LB@ 69 33@LB@@LB@@LB@Energy -11.56 kcal/mol@LB@Hybridization Energy -24.8584 kcal/mol@LB@Unfolding Energy - mRNA 8.52016 kcal/mol@LB@Unfolding Energy - sRNA 4.78423 kcal/mol@LB@@LB@Position - mRNA 72 -- 108@LB@Position - sRNA 34 -- 68@LB@Position Seed - mRNA 74 -- 80@LB@Position Seed - sRNA 60 -- 66@LB@
53	0.001859	0.1218	b0529	folD	-11.41	0.136116	200 -- 226	61 -- 85	bifunctional 5 10-methylene-tetrahydrofolate dehydrogenase/ 5 10-methylene-tetrahydrofolate cyclohydrolase		_____________199_________________________227\n_____________\|___________________________\|\n5'-CAA...UCUCU_____CA___________UG_______AACGA...CAC-3'\n______________GAUGG__GCAAAGAUUAU__ACGGUAA\n______________\|\|\|\|\|__\|\|\|\|\|\|\|:\|\|:__\|\|:\|:\|\|\n______________CUACC__CGUUUCUGAUG__UGUCGUU\n3'-UUU...ACCCU__________________UG_______AAGCA...GCA-5'\n_____________\|___________________________\|\n_____________86__________________________60\n	220 -- 226	61 -- 67	-27.4215 kcal/mol	5.77154 kcal/mol	10.2439 kcal/mol	199 227@LB@ \| \|@LB@5'-CAA...UCUCU CA UG AACGA...CAC-3'@LB@ GAUGG GCAAAGAUUAU ACGGUAA@LB@ \|\|\|\|\| \|\|\|\|\|\|\|:\|\|: \|\|:\|:\|\|@LB@ CUACC CGUUUCUGAUG UGUCGUU@LB@3'-UUU...ACCCU UG AAGCA...GCA-5'@LB@ \| \|@LB@ 86 60@LB@@LB@@LB@Energy -11.41 kcal/mol@LB@Hybridization Energy -27.4215 kcal/mol@LB@Unfolding Energy - mRNA 5.77154 kcal/mol@LB@Unfolding Energy - sRNA 10.2439 kcal/mol@LB@@LB@Position - mRNA 200 -- 226@LB@Position - sRNA 61 -- 85@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 61 -- 67@LB@
54	0.00187	0.1218	b3607	cysE	-14.87	0.022582	98 -- 129	42 -- 78	serine acetyltransferase		_____________97_________________________________________130\n_____________\|__________________________________________\|\n5'-CGG...CACGC______GA__________C_____________AACCC_____AACCU...CGA-3'\n______________AAGGAC___GCGCAGCAG__________CACU_____CAGGG\n______________\|\|:\|\|\|___:\|:\|\|\|\|\|:__________\|\|\|\|_____\|\|\|\|\|\n______________UUUCUG___UGUGUCGUU__________GUGA_____GUCCC\n3'-UUU...ACCCG______AUG_________AAGCAACAAA______________CUAAA...GCA-5'\n_____________\|__________________________________________\|\n_____________79_________________________________________41\n	108 -- 114	61 -- 67	-27.0645 kcal/mol	7.42824 kcal/mol	4.76487 kcal/mol	97 130@LB@ \| \|@LB@5'-CGG...CACGC GA C AACCC AACCU...CGA-3'@LB@ AAGGAC GCGCAGCAG CACU CAGGG@LB@ \|\|:\|\|\| :\|:\|\|\|\|\|: \|\|\|\| \|\|\|\|\|@LB@ UUUCUG UGUGUCGUU GUGA GUCCC@LB@3'-UUU...ACCCG AUG AAGCAACAAA CUAAA...GCA-5'@LB@ \| \|@LB@ 79 41@LB@@LB@@LB@Energy -14.87 kcal/mol@LB@Hybridization Energy -27.0645 kcal/mol@LB@Unfolding Energy - mRNA 7.42824 kcal/mol@LB@Unfolding Energy - sRNA 4.76487 kcal/mol@LB@@LB@Position - mRNA 98 -- 129@LB@Position - sRNA 42 -- 78@LB@Position Seed - mRNA 108 -- 114@LB@Position Seed - sRNA 61 -- 67@LB@
55	0.001955	0.1223	b0840	deoR	-10.34	0.214583	216 -- 260	60 -- 103	deoxyribose-5-phosphate-inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx		_____________215____________________________________________261\n_____________\|______________________________________________\|\n5'-GUG...GUCGC_______G________C__C__CU_____A____AA__________AAGUU...UUU-3'\n______________GAAGAGC_UAUCG_GG_AG_UG__GCAAG_AUUA__ACGCAGCGAU\n______________:\|\|:\|\|\|_:\|\|\|\|_\|\|_\|\|_\|\|__\|\|\|\|:_\|:\|\|__\|\|:\|\|\|\|:\|\|\n______________UUUUUCG_GUAGC_CC_UC_AC__CGUUU_UGAU__UGUGUCGUUA\n_________3'-UU_______G_____A__C__U__C______C____G___________AGCAA...GCA-5'\n_____________\|______________________________________________\|\n_____________104____________________________________________59\n	254 -- 260	60 -- 66	-39.4084 kcal/mol	12.9673 kcal/mol	16.1071 kcal/mol	215 261@LB@ \| \|@LB@5'-GUG...GUCGC G C C CU A AA AAGUU...UUU-3'@LB@ GAAGAGC UAUCG GG AG UG GCAAG AUUA ACGCAGCGAU@LB@ :\|\|:\|\|\| :\|\|\|\| \|\| \|\| \|\| \|\|\|\|: \|:\|\| \|\|:\|\|\|\|:\|\|@LB@ UUUUUCG GUAGC CC UC AC CGUUU UGAU UGUGUCGUUA@LB@ 3'-UU G A C U C C G AGCAA...GCA-5'@LB@ \| \|@LB@ 104 59@LB@@LB@@LB@Energy -10.34 kcal/mol@LB@Hybridization Energy -39.4084 kcal/mol@LB@Unfolding Energy - mRNA 12.9673 kcal/mol@LB@Unfolding Energy - sRNA 16.1071 kcal/mol@LB@@LB@Position - mRNA 216 -- 260@LB@Position - sRNA 60 -- 103@LB@Position Seed - mRNA 254 -- 260@LB@Position Seed - sRNA 60 -- 66@LB@
56	0.001982	0.1223	b2304	yfcH	-16.49	0.008143	193 -- 225	40 -- 77	putative NAD-dependent nucleotide-sugar epimerase		_____________192_________________________________________226\n_____________\|___________________________________________\|\n5'-AAA...AUCGG________AUGA__________________________A____CAGGA...ACC-3'\n______________GAGGCUGC____ACAUAGUGA_________UCAC_CGG_GGGA\n______________:\|\|:\|\|:\|____\|\|\|:\|\|::\|_________\|\|\|\|_\|:\|_\|\|\|\|\n______________UUCUGAUG____UGUGUCGUU_________AGUG_GUC_CCCU\n3'-UUU...CCCGU_____________________AAGCAACAA____A________AAAGG...GCA-5'\n_____________\|___________________________________________\|\n_____________78__________________________________________39\n	206 -- 212	62 -- 68	-29.217 kcal/mol	7.9505 kcal/mol	4.77871 kcal/mol	192 226@LB@ \| \|@LB@5'-AAA...AUCGG AUGA A CAGGA...ACC-3'@LB@ GAGGCUGC ACAUAGUGA UCAC CGG GGGA@LB@ :\|\|:\|\|:\| \|\|\|:\|\|::\| \|\|\|\| \|:\| \|\|\|\|@LB@ UUCUGAUG UGUGUCGUU AGUG GUC CCCU@LB@3'-UUU...CCCGU AAGCAACAA A AAAGG...GCA-5'@LB@ \| \|@LB@ 78 39@LB@@LB@@LB@Energy -16.49 kcal/mol@LB@Hybridization Energy -29.217 kcal/mol@LB@Unfolding Energy - mRNA 7.9505 kcal/mol@LB@Unfolding Energy - sRNA 4.77871 kcal/mol@LB@@LB@Position - mRNA 193 -- 225@LB@Position - sRNA 40 -- 77@LB@Position Seed - mRNA 206 -- 212@LB@Position Seed - sRNA 62 -- 68@LB@
57	0.002011	0.1223	b3057	bacA	-16.67	0.007222	149 -- 193	41 -- 71	undecaprenyl pyrophosphate phosphatase		_____________148___________________________________________194\n_____________\|_____________________________________________\|\n5'-UUC...UGGUC_______________GACAAG_GGUACAUUUUAAUA_________UUUAU...UUG-3'\n______________AUACGCAGUAGUUCG______C______________AUUUAGGGG\n______________\|:\|\|:\|\|\|:\|:\|\|\|\|______\|______________\|:\|:\|\|\|\|\|\n______________UGUGUGUCGUUAAGC______G______________UGAGUCCCC\n3'-UUU...UCUGA_______________AACAAA________________________UAAAG...GCA-5'\n_____________\|_____________________________________________\|\n_____________72____________________________________________40\n	187 -- 193	41 -- 47	-30.087 kcal/mol	8.67993 kcal/mol	4.74226 kcal/mol	148 194@LB@ \| \|@LB@5'-UUC...UGGUC GACAAG GGUACAUUUUAAUA UUUAU...UUG-3'@LB@ AUACGCAGUAGUUCG C AUUUAGGGG@LB@ \|:\|\|:\|\|\|:\|:\|\|\|\| \| \|:\|:\|\|\|\|\|@LB@ UGUGUGUCGUUAAGC G UGAGUCCCC@LB@3'-UUU...UCUGA AACAAA UAAAG...GCA-5'@LB@ \| \|@LB@ 72 40@LB@@LB@@LB@Energy -16.67 kcal/mol@LB@Hybridization Energy -30.087 kcal/mol@LB@Unfolding Energy - mRNA 8.67993 kcal/mol@LB@Unfolding Energy - sRNA 4.74226 kcal/mol@LB@@LB@Position - mRNA 149 -- 193@LB@Position - sRNA 41 -- 71@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 41 -- 47@LB@
58	0.002015	0.1223	b0189	rof	-8.06	0.473679	29 -- 61	40 -- 71	modulator of Rho-dependent transcription termination		_____________28_________________________________62\n_____________\|__________________________________\|\n5'-AGG...AGUUA_______A_____A__CG_AAA________C___GACUU...GCG-3'\n______________AUACGCA_GCGGU_CG__G___UCGC_CAG_GGA\n______________\|:\|\|:\|\|_\|\|::\|_\|\|__\|___\|\|:\|_\|\|\|_\|\|\|\n______________UGUGUGU_CGUUA_GC__C___AGUG_GUC_CCU\n3'-UUU...UCUGA_____________A__AA_AA_____A___C___AAAGG...GCA-5'\n_____________\|__________________________________\|\n_____________72_________________________________39\n	29 -- 35	65 -- 71	-25.5885 kcal/mol	12.7864 kcal/mol	4.74818 kcal/mol	28 62@LB@ \| \|@LB@5'-AGG...AGUUA A A CG AAA C GACUU...GCG-3'@LB@ AUACGCA GCGGU CG G UCGC CAG GGA@LB@ \|:\|\|:\|\| \|\|::\| \|\| \| \|\|:\| \|\|\| \|\|\|@LB@ UGUGUGU CGUUA GC C AGUG GUC CCU@LB@3'-UUU...UCUGA A AA AA A C AAAGG...GCA-5'@LB@ \| \|@LB@ 72 39@LB@@LB@@LB@Energy -8.06 kcal/mol@LB@Hybridization Energy -25.5885 kcal/mol@LB@Unfolding Energy - mRNA 12.7864 kcal/mol@LB@Unfolding Energy - sRNA 4.74818 kcal/mol@LB@@LB@Position - mRNA 29 -- 61@LB@Position - sRNA 40 -- 71@LB@Position Seed - mRNA 29 -- 35@LB@Position Seed - sRNA 65 -- 71@LB@
59	0.002304	0.1363	b2508	guaB	-11.36	0.139274	1 -- 26	38 -- 71	IMP dehydrogenase		______________1__________________________________27\n______________\|__________________________________\|\n___________5'-__UA_______AA____________G_________GAGAA...AGC-3'\n______________AC__ACGGUAA___________AUU_CAGGGGAUU\n______________\|\|__\|\|:\|:\|\|___________\|:\|_\|\|\|\|\|\|\|\|\|\n______________UG__UGUCGUU___________UGA_GUCCCCUAA\n3'-UUU...UCUGA__UG_______AAGCAACAAAG_____________AGGUA...GCA-5'\n_____________\|___________________________________\|\n_____________72__________________________________37\n	20 -- 26	38 -- 44	-21.1303 kcal/mol	5.02575 kcal/mol	4.74986 kcal/mol	1 27@LB@ \| \|@LB@ 5'- UA AA G GAGAA...AGC-3'@LB@ AC ACGGUAA AUU CAGGGGAUU@LB@ \|\| \|\|:\|:\|\| \|:\| \|\|\|\|\|\|\|\|\|@LB@ UG UGUCGUU UGA GUCCCCUAA@LB@3'-UUU...UCUGA UG AAGCAACAAAG AGGUA...GCA-5'@LB@ \| \|@LB@ 72 37@LB@@LB@@LB@Energy -11.36 kcal/mol@LB@Hybridization Energy -21.1303 kcal/mol@LB@Unfolding Energy - mRNA 5.02575 kcal/mol@LB@Unfolding Energy - sRNA 4.74986 kcal/mol@LB@@LB@Position - mRNA 1 -- 26@LB@Position - sRNA 38 -- 71@LB@Position Seed - mRNA 20 -- 26@LB@Position Seed - sRNA 38 -- 44@LB@
60	0.002324	0.1363	b0881	clpS	-11.74	0.117068	9 -- 15	40 -- 46	regulatory protein for ClpA substrate specificity		_____________8_______16\n_____________\|_______\|\n___5'-GCAUUUGA_______CAAGG...UCA-3'\n______________CAGGGGA\n______________\|\|\|\|\|\|\|\n______________GUCCCCU\n3'-UUU...AGUGA_______AAAGG...GCA-5'\n_____________\|_______\|\n_____________47______39\n	9 -- 15	40 -- 46	-13.2936 kcal/mol	0.199257 kcal/mol	1.35582 kcal/mol	8 16@LB@ \| \|@LB@ 5'-GCAUUUGA CAAGG...UCA-3'@LB@ CAGGGGA@LB@ \|\|\|\|\|\|\|@LB@ GUCCCCU@LB@3'-UUU...AGUGA AAAGG...GCA-5'@LB@ \| \|@LB@ 47 39@LB@@LB@@LB@Energy -11.74 kcal/mol@LB@Hybridization Energy -13.2936 kcal/mol@LB@Unfolding Energy - mRNA 0.199257 kcal/mol@LB@Unfolding Energy - sRNA 1.35582 kcal/mol@LB@@LB@Position - mRNA 9 -- 15@LB@Position - sRNA 40 -- 46@LB@Position Seed - mRNA 9 -- 15@LB@Position Seed - sRNA 40 -- 46@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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