Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
121	0.01323	0.3575	b3429	glgA	-14.56	0.033318	190 -- 240	123 -- 176	glycogen synthase	_____________189__________________241\n_____________\|____________________\|\n5'-GUA...AUGCA__________GU________AGAUG...ACG-3'\n______________GGUUUUACAU__AUGUUCAG\n______________\|:\|\|\|\|\|\|\|:__\|\|::\|\|\|\|\n______________CUAAAAUGUG__UAUGAGUC\n3'-UUU...GCCCA__________GU________AGUGU...UAG-5'\n_____________\|____________________\|\n_____________177__________________122\n	219 -- 225	174 -- 180	-15.9439 kcal/mol	3.46677 kcal/mol	4.94566 kcal/mol	189 241@LB@ \| \|@LB@5'-GUA...AUGCA GU AGAUG...ACG-3'@LB@ GGUUUUACAU AUGUUCAG@LB@ \|:\|\|\|\|\|\|\|: \|\|::\|\|\|\|@LB@ CUAAAAUGUG UAUGAGUC@LB@3'-UUU...GCCCA GU AGUGU...UAG-5'@LB@ \| \|@LB@ 177 122@LB@@LB@@LB@Energy -14.56 kcal/mol@LB@Hybridization Energy -15.9439 kcal/mol@LB@Unfolding Energy - mRNA 3.46677 kcal/mol@LB@Unfolding Energy - sRNA 4.94566 kcal/mol@LB@@LB@Position - mRNA 190 -- 240@LB@Position - sRNA 123 -- 176@LB@Position Seed - mRNA 219 -- 225@LB@Position Seed - sRNA 174 -- 180@LB@
104	0.01113	0.3575	b2108	yehA	-14.54	0.033608	284 -- 300	56 -- 72	putative fimbrial-like adhesin protein	_____________283_____________300\n_____________\|_______________\|\n5'-GUU...CUGUU___________G____-3'\n______________CAGCACAACUU_GUUG\n______________\|\|\|\|\|\|\|\|\|\|\|_\|:\|\|\n______________GUCGUGUUGAA_CGAC\n3'-UUU...CGUUG___________G____AGCGC...UAG-5'\n_____________\|________________\|\n_____________73_______________55\n	289 -- 295	61 -- 67	-24.1215 kcal/mol	2.36332 kcal/mol	11.1531 kcal/mol	283 300@LB@ \| \|@LB@5'-GUU...CUGUU G -3'@LB@ CAGCACAACUU GUUG@LB@ \|\|\|\|\|\|\|\|\|\|\| \|:\|\|@LB@ GUCGUGUUGAA CGAC@LB@3'-UUU...CGUUG G AGCGC...UAG-5'@LB@ \| \|@LB@ 73 55@LB@@LB@@LB@Energy -14.54 kcal/mol@LB@Hybridization Energy -24.1215 kcal/mol@LB@Unfolding Energy - mRNA 2.36332 kcal/mol@LB@Unfolding Energy - sRNA 11.1531 kcal/mol@LB@@LB@Position - mRNA 284 -- 300@LB@Position - sRNA 56 -- 72@LB@Position Seed - mRNA 289 -- 295@LB@Position Seed - sRNA 61 -- 67@LB@
155	0.01733	0.3749	b3542	dppC	-14.36	0.036537	40 -- 62	74 -- 93	dipeptide/heme ABC transporter permease	_____________39_______________________________________________________63\n_____________\|________________________________________________________\|\n5'-GGG...UCGUC____U__U___C__________UA___C_____GA_______C_____________AGAAG...UCG-3'\n______________AACU_GC_GGU__GAUCUGCUG__CGG_GUGGU___AC_CCG_GUAUUC_GUCAUA\n______________\|\|\|:_\|\|_\|\|\|__:\|\|\|\|\|\|\|\|__\|\|\|_\|\|\|:\|___\|\|_\|\|:_:\|\|:\|\|_\|\|\|\|:\|\n______________UUGG_CG_CCA__UUAGACGAC__GCC_CACUA___UG_GGU_UAUGAG_CAGUGU\n_____3'-UUUUUU____U__U___UA_________C__________AAA__U__________U______GUACU...UAG-5'\n_____________\|________________________________________________________\|\n_____________94_______________________________________________________73\n	146 -- 152	200 -- 206	-46.3163 kcal/mol	15.5571 kcal/mol	18.3074 kcal/mol	39 63@LB@ \| \|@LB@5'-GGG...UCGUC U U C UA C GA C AGAAG...UCG-3'@LB@ AACU GC GGU GAUCUGCUG CGG GUGGU AC CCG GUAUUC GUCAUA@LB@ \|\|\|: \|\| \|\|\| :\|\|\|\|\|\|\|\| \|\|\| \|\|\|:\| \|\| \|\|: :\|\|:\|\| \|\|\|\|:\|@LB@ UUGG CG CCA UUAGACGAC GCC CACUA UG GGU UAUGAG CAGUGU@LB@ 3'-UUUUUU U U UA C AAA U U GUACU...UAG-5'@LB@ \| \|@LB@ 94 73@LB@@LB@@LB@Energy -14.36 kcal/mol@LB@Hybridization Energy -46.3163 kcal/mol@LB@Unfolding Energy - mRNA 15.5571 kcal/mol@LB@Unfolding Energy - sRNA 18.3074 kcal/mol@LB@@LB@Position - mRNA 40 -- 62@LB@Position - sRNA 74 -- 93@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 200 -- 206@LB@
116	0.01276	0.3575	b2989	yghU	-14.25	0.038539	247 -- 281	170 -- 208	putative S-transferase	_____________246______________________________________282\n_____________\|________________________________________\|\n5'-UUA...GGAUA____C________C_C_UU________________A____CCGGU...CGC-3'\n______________AAUC_GCUGGCGG_G_G______CGCCAAUA_UCA_UCGC\n______________\|\|\|\|_\|\|\|\|\|\|\|\|_\|_\|______\|:\|\|\|\|\|\|_\|\|\|_\|\|:\|\n______________UUAG_CGACCGCC_C_C______GUGGUUAU_AGU_AGUG\n3'-UUU...CCAUA____A__________A_UAAAAU________G___C____UGUAC...UAG-5'\n_____________\|________________________________________\|\n_____________209______________________________________169\n	267 -- 273	179 -- 185	-35.9489 kcal/mol	9.63265 kcal/mol	15.256 kcal/mol	246 282@LB@ \| \|@LB@5'-UUA...GGAUA C C C UU A CCGGU...CGC-3'@LB@ AAUC GCUGGCGG G G CGCCAAUA UCA UCGC@LB@ \|\|\|\| \|\|\|\|\|\|\|\| \| \| \|:\|\|\|\|\|\| \|\|\| \|\|:\|@LB@ UUAG CGACCGCC C C GUGGUUAU AGU AGUG@LB@3'-UUU...CCAUA A A UAAAAU G C UGUAC...UAG-5'@LB@ \| \|@LB@ 209 169@LB@@LB@@LB@Energy -14.25 kcal/mol@LB@Hybridization Energy -35.9489 kcal/mol@LB@Unfolding Energy - mRNA 9.63265 kcal/mol@LB@Unfolding Energy - sRNA 15.256 kcal/mol@LB@@LB@Position - mRNA 247 -- 281@LB@Position - sRNA 170 -- 208@LB@Position Seed - mRNA 267 -- 273@LB@Position Seed - sRNA 179 -- 185@LB@
80	0.008443	0.3551	b3791	wecE	-14.19	0.039608	22 -- 66	14 -- 64	TDP-4-oxo-6-deoxy-D-glucose transaminase	_____________21______________67\n_____________\|_______________\|\n5'-GAC...ACCGC_______________GGUGA...CCC-3'\n______________GUACU_GGUUAUACA\n______________:\|\|\|\|_:\|\|:\|:\|\|\|\n______________UAUGA_UCAGUGUGU\n3'-UUU...GUGGU_____G_________ACUAC...UAG-5'\n_____________\|_______________\|\n_____________65______________13\n	185 -- 191	167 -- 173	-12.5686 kcal/mol	2.73267 kcal/mol	0.435744 kcal/mol	21 67@LB@ \| \|@LB@5'-GAC...ACCGC GGUGA...CCC-3'@LB@ GUACU GGUUAUACA@LB@ :\|\|\|\| :\|\|:\|:\|\|\|@LB@ UAUGA UCAGUGUGU@LB@3'-UUU...GUGGU G ACUAC...UAG-5'@LB@ \| \|@LB@ 65 13@LB@@LB@@LB@Energy -14.19 kcal/mol@LB@Hybridization Energy -12.5686 kcal/mol@LB@Unfolding Energy - mRNA 2.73267 kcal/mol@LB@Unfolding Energy - sRNA 0.435744 kcal/mol@LB@@LB@Position - mRNA 22 -- 66@LB@Position - sRNA 14 -- 64@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 167 -- 173@LB@
68	0.00659	0.3261	b4080	mdtP	-14.15	0.040423	195 -- 221	144 -- 172	outer membrane factor of efflux pump	_____________194_______________________________222\n_____________\|_________________________________\|\n5'-CUG...CAAGG__C_____A________AUC_____________CGUCU...CUC-3'\n______________AG_CACGC_AUGAU_CA_____GUCAACUUUCA\n______________\|\|_\|\|\|:\|_\|\|\|\|\|_\|\|_____\|\|\|\|\|\|\|\|\|\|\|\n______________UC_GUGUG_UACUA_GU_____CAGUUGAAAGU\n3'-UUU...AUGAG__A___________C__CCGUU___________CUUAA...UAG-5'\n_____________\|_________________________________\|\n_____________173_______________________________143\n	215 -- 221	144 -- 150	-24.8993 kcal/mol	3.71866 kcal/mol	8.07675 kcal/mol	194 222@LB@ \| \|@LB@5'-CUG...CAAGG C A AUC CGUCU...CUC-3'@LB@ AG CACGC AUGAU CA GUCAACUUUCA@LB@ \|\| \|\|\|:\| \|\|\|\|\| \|\| \|\|\|\|\|\|\|\|\|\|\|@LB@ UC GUGUG UACUA GU CAGUUGAAAGU@LB@3'-UUU...AUGAG A C CCGUU CUUAA...UAG-5'@LB@ \| \|@LB@ 173 143@LB@@LB@@LB@Energy -14.15 kcal/mol@LB@Hybridization Energy -24.8993 kcal/mol@LB@Unfolding Energy - mRNA 3.71866 kcal/mol@LB@Unfolding Energy - sRNA 8.07675 kcal/mol@LB@@LB@Position - mRNA 195 -- 221@LB@Position - sRNA 144 -- 172@LB@Position Seed - mRNA 215 -- 221@LB@Position Seed - sRNA 144 -- 150@LB@
17	0.001014	0.2007	b4032	malG	-14.09	0.041561	127 -- 139	193 -- 205	maltose transporter subunit	_____________126___________140\n_____________\|_____________\|\n5'-CGU...UCUUC_____________CGCUG...UGG-3'\n______________CUGCUGGUGGGUG\n______________\|\|\|\|\|\|\|:\|\|\|\|\|\n______________GACGACCGCCCAC\n3'-UUU...UAUUA_____________UAAAA...UAG-5'\n_____________\|_____________\|\n_____________206___________192\n	132 -- 138	194 -- 200	-26.9183 kcal/mol	7.69341 kcal/mol	9.06058 kcal/mol	126 140@LB@ \| \|@LB@5'-CGU...UCUUC CGCUG...UGG-3'@LB@ CUGCUGGUGGGUG@LB@ \|\|\|\|\|\|\|:\|\|\|\|\|@LB@ GACGACCGCCCAC@LB@3'-UUU...UAUUA UAAAA...UAG-5'@LB@ \| \|@LB@ 206 192@LB@@LB@@LB@Energy -14.09 kcal/mol@LB@Hybridization Energy -26.9183 kcal/mol@LB@Unfolding Energy - mRNA 7.69341 kcal/mol@LB@Unfolding Energy - sRNA 9.06058 kcal/mol@LB@@LB@Position - mRNA 127 -- 139@LB@Position - sRNA 193 -- 205@LB@Position Seed - mRNA 132 -- 138@LB@Position Seed - sRNA 194 -- 200@LB@
105	0.01118	0.3575	b0439	lon	-14.09	0.041610	272 -- 293	1 -- 24	DNA-binding ATP-dependent protease La	_____________271_______294\n_____________\|_________\|\n5'-AUC...CACAU_________CUUAU...GGG-3'\n______________GGUCAUCCC\n______________\|\|\|\|\|\|\|\|\|\n______________CCAGUAGGG\n3'-UUU...UAACG_________UGACG...UAG-5'\n_____________\|_________\|\n_____________25________0\n	277 -- 283	128 -- 134	-16.7868 kcal/mol	1.03204 kcal/mol	4.55487 kcal/mol	271 294@LB@ \| \|@LB@5'-AUC...CACAU CUUAU...GGG-3'@LB@ GGUCAUCCC@LB@ \|\|\|\|\|\|\|\|\|@LB@ CCAGUAGGG@LB@3'-UUU...UAACG UGACG...UAG-5'@LB@ \| \|@LB@ 25 0@LB@@LB@@LB@Energy -14.09 kcal/mol@LB@Hybridization Energy -16.7868 kcal/mol@LB@Unfolding Energy - mRNA 1.03204 kcal/mol@LB@Unfolding Energy - sRNA 4.55487 kcal/mol@LB@@LB@Position - mRNA 272 -- 293@LB@Position - sRNA 1 -- 24@LB@Position Seed - mRNA 277 -- 283@LB@Position Seed - sRNA 128 -- 134@LB@
169	0.01937	0.3812	b4557	yidD	-14.07	0.041992	2 -- 13	11 -- 22	membrane protein insertion efficiency factor UPF0161 family inner membrane protein	_____________1______________________14\n_____________\|______________________\|\n__________5'-C____________GUAUCG____UUACA...UCA-3'\n______________UGGGGCAUCCCC_______GUC\n______________\|\|\|\|\|\|\|:\|\|\|\|_______:\|\|\n______________ACCCCGUGGGGG_______UAG\n3'-UUU...UGCGU____________AACGAAG___-5'\n_____________\|_____________________\|\n_____________23____________________11\n	7 -- 13	11 -- 17	-28.608 kcal/mol	8.24927 kcal/mol	10.7363 kcal/mol	1 14@LB@ \| \|@LB@ 5'-C GUAUCG UUACA...UCA-3'@LB@ UGGGGCAUCCCC GUC@LB@ \|\|\|\|\|\|\|:\|\|\|\| :\|\|@LB@ ACCCCGUGGGGG UAG@LB@3'-UUU...UGCGU AACGAAG -5'@LB@ \| \|@LB@ 23 11@LB@@LB@@LB@Energy -14.07 kcal/mol@LB@Hybridization Energy -28.608 kcal/mol@LB@Unfolding Energy - mRNA 8.24927 kcal/mol@LB@Unfolding Energy - sRNA 10.7363 kcal/mol@LB@@LB@Position - mRNA 2 -- 13@LB@Position - sRNA 11 -- 22@LB@Position Seed - mRNA 7 -- 13@LB@Position Seed - sRNA 11 -- 17@LB@
34	0.002769	0.2736	b2416	ptsI	-14.05	0.042463	178 -- 225	128 -- 177	PEP-protein phosphotransferase of PTS system (enzyme I)	_____________177_________________________________________226\n_____________\|___________________________________________\|\n5'-GUG...AAAAA_________AGUAAGGUAGGGU______UU______UU_____AUCCC...CCG-3'\n______________UCAGUCACA_____________UAUGAU__CAGGCA__UUAGC\n______________\|\|\|\|\|\|\|\|\|_____________:\|\|\|\|\|__\|\|\|\|\|\|__\|:\|:\|\n______________AGUCAGUGU_____________GUACUA__GUCCGU__AGUUG\n3'-UUU...UUAUG____________________________C_______UC_____AAAGU...UAG-5'\n_____________\|___________________________________________\|\n_____________178_________________________________________127\n	180 -- 186	169 -- 175	-30.6202 kcal/mol	5.78851 kcal/mol	8.17404 kcal/mol	177 226@LB@ \| \|@LB@5'-GUG...AAAAA AGUAAGGUAGGGU UU UU AUCCC...CCG-3'@LB@ UCAGUCACA UAUGAU CAGGCA UUAGC@LB@ \|\|\|\|\|\|\|\|\| :\|\|\|\|\| \|\|\|\|\|\| \|:\|:\|@LB@ AGUCAGUGU GUACUA GUCCGU AGUUG@LB@3'-UUU...UUAUG C UC AAAGU...UAG-5'@LB@ \| \|@LB@ 178 127@LB@@LB@@LB@Energy -14.05 kcal/mol@LB@Hybridization Energy -30.6202 kcal/mol@LB@Unfolding Energy - mRNA 5.78851 kcal/mol@LB@Unfolding Energy - sRNA 8.17404 kcal/mol@LB@@LB@Position - mRNA 178 -- 225@LB@Position - sRNA 128 -- 177@LB@Position Seed - mRNA 180 -- 186@LB@Position Seed - sRNA 169 -- 175@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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