Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
132	0.0143	0.3575	b0147	ligT	-9.89	0.288456	69 -- 117	16 -- 69	2'-5' RNA ligase	_____________68__________________________________118\n_____________\|___________________________________\|\n5'-CCA...AAGUA______GUA_G___GCAGCAA_______C_A____ACAAA...CGG-3'\n______________AUUCAG___A_ACG_______CGGGCAA_G_CGAC\n______________\|:\|\|\|\|___\|_\|\|:_______\|:\|\|\|\|\|_\|_\|:\|\|\n______________UGAGUC___U_UGU_______GUCCGUU_C_GUUG\n3'-UUU...GGUUA______AG__G___ACUAC___________A____AAAGU...UAG-5'\n_____________\|___________________________________\|\n_____________70__________________________________15\n	112 -- 118	155 -- 161	-21.4956 kcal/mol	4.52534 kcal/mol	8.18567 kcal/mol	68 118@LB@ \| \|@LB@5'-CCA...AAGUA GUA G GCAGCAA C A ACAAA...CGG-3'@LB@ AUUCAG A ACG CGGGCAA G CGAC@LB@ \|:\|\|\|\| \| \|\|: \|:\|\|\|\|\| \| \|:\|\|@LB@ UGAGUC U UGU GUCCGUU C GUUG@LB@3'-UUU...GGUUA AG G ACUAC A AAAGU...UAG-5'@LB@ \| \|@LB@ 70 15@LB@@LB@@LB@Energy -9.89 kcal/mol@LB@Hybridization Energy -21.4956 kcal/mol@LB@Unfolding Energy - mRNA 4.52534 kcal/mol@LB@Unfolding Energy - sRNA 8.18567 kcal/mol@LB@@LB@Position - mRNA 69 -- 117@LB@Position - sRNA 16 -- 69@LB@Position Seed - mRNA 112 -- 118@LB@Position Seed - sRNA 155 -- 161@LB@
25	0.002009	0.2633	b0134	panB	-9.88	0.290153	57 -- 67	176 -- 186	3-methyl-2-oxobutanoate hydroxymethyltransferase	_____________56__________68\n_____________\|___________\|\n5'-CAG...AAAGC___________UCCAG...UUG-3'\n______________CACCAGUACUU\n______________\|\|\|\|\|:\|\|\|\|:\n______________GUGGUUAUGAG\n3'-UUU...AAAAU___________UCAGU...UAG-5'\n_____________\|___________\|\n_____________187_________175\n	61 -- 67	176 -- 182	-15.2136 kcal/mol	0.973349 kcal/mol	3.38772 kcal/mol	56 68@LB@ \| \|@LB@5'-CAG...AAAGC UCCAG...UUG-3'@LB@ CACCAGUACUU@LB@ \|\|\|\|\|:\|\|\|\|:@LB@ GUGGUUAUGAG@LB@3'-UUU...AAAAU UCAGU...UAG-5'@LB@ \| \|@LB@ 187 175@LB@@LB@@LB@Energy -9.88 kcal/mol@LB@Hybridization Energy -15.2136 kcal/mol@LB@Unfolding Energy - mRNA 0.973349 kcal/mol@LB@Unfolding Energy - sRNA 3.38772 kcal/mol@LB@@LB@Position - mRNA 57 -- 67@LB@Position - sRNA 176 -- 186@LB@Position Seed - mRNA 61 -- 67@LB@Position Seed - sRNA 176 -- 182@LB@
49	0.004667	0.3205	b1277	ribA	-9.86	0.291903	241 -- 249	16 -- 24	GTP cyclohydrolase II	_____________240________250\n_____________\|__________\|\n5'-CCA...AAUAC__________UUAUC...UCG-3'\n______________AGAACCAGCA\n______________\|:\|\|\|\|\|\|\|\|\n______________UUUUGGUCGU\n_______3'-UUUU__________CCAUA...UAG-5'\n_____________\|__________\|\n_____________25_________15\n	178 -- 184	214 -- 220	-13.9783 kcal/mol	2.87719 kcal/mol	5.75438 kcal/mol	240 250@LB@ \| \|@LB@5'-CCA...AAUAC UUAUC...UCG-3'@LB@ AGAACCAGCA@LB@ \|:\|\|\|\|\|\|\|\|@LB@ UUUUGGUCGU@LB@ 3'-UUUU CCAUA...UAG-5'@LB@ \| \|@LB@ 25 15@LB@@LB@@LB@Energy -9.86 kcal/mol@LB@Hybridization Energy -13.9783 kcal/mol@LB@Unfolding Energy - mRNA 2.87719 kcal/mol@LB@Unfolding Energy - sRNA 5.75438 kcal/mol@LB@@LB@Position - mRNA 241 -- 249@LB@Position - sRNA 16 -- 24@LB@Position Seed - mRNA 178 -- 184@LB@Position Seed - sRNA 214 -- 220@LB@
81	0.008673	0.3575	b1683	sufB	-9.84	0.294156	211 -- 257	124 -- 171	component of SufBCD Fe-S cluster assembly scaffold	_____________210_______________258\n_____________\|_________________\|\n5'-GUU...AAUCA________AAUU_____ACCCU...AGC-3'\n______________GAUAUUCA____UCACA\n______________:\|\|\|:\|\|\|____\|\|\|\|\|\n______________UUAUGAGU____AGUGU\n3'-UUU...UGUGG________C________GUACU...UAG-5'\n_____________\|_________________\|\n_____________172_______________123\n	127 -- 133	175 -- 181	-8.93573 kcal/mol	1.46068 kcal/mol	0.409577 kcal/mol	210 258@LB@ \| \|@LB@5'-GUU...AAUCA AAUU ACCCU...AGC-3'@LB@ GAUAUUCA UCACA@LB@ :\|\|\|:\|\|\| \|\|\|\|\|@LB@ UUAUGAGU AGUGU@LB@3'-UUU...UGUGG C GUACU...UAG-5'@LB@ \| \|@LB@ 172 123@LB@@LB@@LB@Energy -9.84 kcal/mol@LB@Hybridization Energy -8.93573 kcal/mol@LB@Unfolding Energy - mRNA 1.46068 kcal/mol@LB@Unfolding Energy - sRNA 0.409577 kcal/mol@LB@@LB@Position - mRNA 211 -- 257@LB@Position - sRNA 124 -- 171@LB@Position Seed - mRNA 127 -- 133@LB@Position Seed - sRNA 175 -- 181@LB@
77	0.007981	0.3458	b1738	chbB	-9.80	0.299184	58 -- 71	174 -- 187	N N'-diacetylchitobiose-specific enzyme IIB component of PTS	_____________57_______________________________72\n_____________\|________________________________\|\n5'-AAU...UAUAA___CAAAGAA______U_______G__U____CGUAU...CGG-3'\n______________GGU_______AUACUA_UGCUCAG_UC_AUAC\n______________\|\|\|_______\|:\|\|:\|_\|:\|\|\|\|\|_\|\|_\|:\|\|\n______________CCA_______UGUGGU_AUGAGUC_AG_UGUG\n3'-UUU...ACCGC___CUAAAA_______U_______________UACUA...UAG-5'\n_____________\|________________________________\|\n_____________188______________________________173\n	65 -- 71	174 -- 180	-20.4225 kcal/mol	4.13376 kcal/mol	5.03959 kcal/mol	57 72@LB@ \| \|@LB@5'-AAU...UAUAA CAAAGAA U G U CGUAU...CGG-3'@LB@ GGU AUACUA UGCUCAG UC AUAC@LB@ \|\|\| \|:\|\|:\| \|:\|\|\|\|\| \|\| \|:\|\|@LB@ CCA UGUGGU AUGAGUC AG UGUG@LB@3'-UUU...ACCGC CUAAAA U UACUA...UAG-5'@LB@ \| \|@LB@ 188 173@LB@@LB@@LB@Energy -9.80 kcal/mol@LB@Hybridization Energy -20.4225 kcal/mol@LB@Unfolding Energy - mRNA 4.13376 kcal/mol@LB@Unfolding Energy - sRNA 5.03959 kcal/mol@LB@@LB@Position - mRNA 58 -- 71@LB@Position - sRNA 174 -- 187@LB@Position Seed - mRNA 65 -- 71@LB@Position Seed - sRNA 174 -- 180@LB@
59	0.005693	0.3247	b1959	yedA	-9.74	0.306961	258 -- 278	1 -- 22	amino acid exporter for phenylalanine threonine	_____________257_______279\n_____________\|_________\|\n5'-UCA...UGAUA_________CAGGU...GCU-3'\n______________CUUGCUUCA\n______________\|\|\|\|\|\|\|\|\|\n______________GAACGAAGU\n3'-UUU...UGGGG_________AG-5'\n_____________\|_________\|\n_____________23________0\n	90 -- 96	3 -- 9	-13.1616 kcal/mol	3.16944 kcal/mol	1.03153 kcal/mol	257 279@LB@ \| \|@LB@5'-UCA...UGAUA CAGGU...GCU-3'@LB@ CUUGCUUCA@LB@ \|\|\|\|\|\|\|\|\|@LB@ GAACGAAGU@LB@3'-UUU...UGGGG AG-5'@LB@ \| \|@LB@ 23 0@LB@@LB@@LB@Energy -9.74 kcal/mol@LB@Hybridization Energy -13.1616 kcal/mol@LB@Unfolding Energy - mRNA 3.16944 kcal/mol@LB@Unfolding Energy - sRNA 1.03153 kcal/mol@LB@@LB@Position - mRNA 258 -- 278@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 90 -- 96@LB@Position Seed - sRNA 3 -- 9@LB@
163	0.01822	0.376	b1073	flgB	-9.73	0.308533	172 -- 220	24 -- 70	flagellar component of cell-proximal portion of basal-body rod	_____________171_______221\n_____________\|_________\|\n5'-UGA...AACAU_________CG-3'\n______________CGCCAAUGC\n______________\|:\|\|\|\|\|:\|\n______________GUGGUUAUG\n3'-UUU...AAAAU_________AGUCA...UAG-5'\n_____________\|_________\|\n_____________71________23\n	292 -- 298	178 -- 184	-11.5881 kcal/mol	1.63005 kcal/mol	3.31726 kcal/mol	171 221@LB@ \| \|@LB@5'-UGA...AACAU CG-3'@LB@ CGCCAAUGC@LB@ \|:\|\|\|\|\|:\|@LB@ GUGGUUAUG@LB@3'-UUU...AAAAU AGUCA...UAG-5'@LB@ \| \|@LB@ 71 23@LB@@LB@@LB@Energy -9.73 kcal/mol@LB@Hybridization Energy -11.5881 kcal/mol@LB@Unfolding Energy - mRNA 1.63005 kcal/mol@LB@Unfolding Energy - sRNA 3.31726 kcal/mol@LB@@LB@Position - mRNA 172 -- 220@LB@Position - sRNA 24 -- 70@LB@Position Seed - mRNA 292 -- 298@LB@Position Seed - sRNA 178 -- 184@LB@
194	0.02283	0.3833	b3823	rhtC	-9.67	0.316154	216 -- 300	113 -- 178	threonine efflux pump	_____________215___________________301\n_____________\|_____________________\|\n5'-AGA...UAUUU____CC_______________CAUUG...GUU-3'\n______________CUCA__GUCGC_CAUGGUGCA\n______________\|\|\|\|__\|\|\|:\|_\|\|\|\|:\|\|\|\|\n______________GAGU__CAGUG_GUACUACGU\n3'-UUU...GUUAU___________U_________CCGUU...UAG-5'\n_____________\|_____________________\|\n_____________179___________________112\n	228 -- 234	161 -- 167	-23.3741 kcal/mol	7.13181 kcal/mol	4.79874 kcal/mol	215 301@LB@ \| \|@LB@5'-AGA...UAUUU CC CAUUG...GUU-3'@LB@ CUCA GUCGC CAUGGUGCA@LB@ \|\|\|\| \|\|\|:\| \|\|\|\|:\|\|\|\|@LB@ GAGU CAGUG GUACUACGU@LB@3'-UUU...GUUAU U CCGUU...UAG-5'@LB@ \| \|@LB@ 179 112@LB@@LB@@LB@Energy -9.67 kcal/mol@LB@Hybridization Energy -23.3741 kcal/mol@LB@Unfolding Energy - mRNA 7.13181 kcal/mol@LB@Unfolding Energy - sRNA 4.79874 kcal/mol@LB@@LB@Position - mRNA 216 -- 300@LB@Position - sRNA 113 -- 178@LB@Position Seed - mRNA 228 -- 234@LB@Position Seed - sRNA 161 -- 167@LB@
115	0.01273	0.3575	b3440	yhhX	-9.67	0.316530	203 -- 215	123 -- 136	putative oxidoreductase	_____________202______________________________216\n_____________\|________________________________\|\n5'-GAC...CGUAU_______AC____AG____A__U____U____CGCAUA-3'\n______________GUACUUA__CGCA___GAU_GC_GGCA_GUCG\n______________:\|\|\|\|:\|__\|:\|\|___\|\|\|_\|\|_\|\|\|\|_\|\|\|:\n______________UAUGAGU__GUGU___CUA_CG_CCGU_CAGU\n3'-UUU...GUGGU_______CA____GUA______U____U____UGAAA...UAG-5'\n_____________\|________________________________\|\n_____________137______________________________122\n	264 -- 270	175 -- 181	-25.3028 kcal/mol	7.53441 kcal/mol	8.15082 kcal/mol	202 216@LB@ \| \|@LB@5'-GAC...CGUAU AC AG A U U CGCAUA-3'@LB@ GUACUUA CGCA GAU GC GGCA GUCG@LB@ :\|\|\|\|:\| \|:\|\| \|\|\| \|\| \|\|\|\| \|\|\|:@LB@ UAUGAGU GUGU CUA CG CCGU CAGU@LB@3'-UUU...GUGGU CA GUA U U UGAAA...UAG-5'@LB@ \| \|@LB@ 137 122@LB@@LB@@LB@Energy -9.67 kcal/mol@LB@Hybridization Energy -25.3028 kcal/mol@LB@Unfolding Energy - mRNA 7.53441 kcal/mol@LB@Unfolding Energy - sRNA 8.15082 kcal/mol@LB@@LB@Position - mRNA 203 -- 215@LB@Position - sRNA 123 -- 136@LB@Position Seed - mRNA 264 -- 270@LB@Position Seed - sRNA 175 -- 181@LB@
181	0.0209	0.3833	b2233	yfaL	-9.66	0.317681	275 -- 285	50 -- 60	adhesin	_____________274______________286\n_____________\|________________\|\n5'-UAU...UCUCU___AA__G________CGGUC...CAU-3'\n______________GCU__CG_UGUCGCGG\n______________:\|\|__\|\|_\|\|\|\|\|\|\|\|\n______________UGA__GC_ACAGCGCC\n3'-UUU...CGUGU___A___G________AUUUU...UAG-5'\n_____________\|________________\|\n_____________61_______________49\n	279 -- 285	50 -- 56	-20.2435 kcal/mol	4.65708 kcal/mol	6.1451 kcal/mol	274 286@LB@ \| \|@LB@5'-UAU...UCUCU AA G CGGUC...CAU-3'@LB@ GCU CG UGUCGCGG@LB@ :\|\| \|\| \|\|\|\|\|\|\|\|@LB@ UGA GC ACAGCGCC@LB@3'-UUU...CGUGU A G AUUUU...UAG-5'@LB@ \| \|@LB@ 61 49@LB@@LB@@LB@Energy -9.66 kcal/mol@LB@Hybridization Energy -20.2435 kcal/mol@LB@Unfolding Energy - mRNA 4.65708 kcal/mol@LB@Unfolding Energy - sRNA 6.1451 kcal/mol@LB@@LB@Position - mRNA 275 -- 285@LB@Position - sRNA 50 -- 60@LB@Position Seed - mRNA 279 -- 285@LB@Position Seed - sRNA 50 -- 56@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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