Input and runtime details for job 1229462 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1229462 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:25:41 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:59 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:22:57 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:23:04 CET 2018
	Job Completed		@ Fri Feb 16 20:23:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1229462&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicF

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
131	0.02082	0.5217	b2038	rfbC	-12.83	0.023839	249 -- 268	2 -- 18	dTDP-4-deoxyrhamnose-3 5-epimerase	_____________248__________________269\n_____________\|____________________\|\n5'-GGG...AGCCA_____AUU____________AGGUU...CAG-3'\n______________AGAGU___UGGUGAUGAUAG\n______________\|:\|\|\|___\|::\|\|\|\|\|\|\|\|\|\n______________UUUCA___AUUACUACUAUC\n3'-UUU...AUUUA____________________G-5'\n_____________\|____________________\|\n_____________19___________________1\n	262 -- 268	2 -- 8	-17.1909 kcal/mol	4.30321 kcal/mol	0.0608678 kcal/mol	248 269@LB@ \| \|@LB@5'-GGG...AGCCA AUU AGGUU...CAG-3'@LB@ AGAGU UGGUGAUGAUAG@LB@ \|:\|\|\| \|::\|\|\|\|\|\|\|\|\|@LB@ UUUCA AUUACUACUAUC@LB@3'-UUU...AUUUA G-5'@LB@ \| \|@LB@ 19 1@LB@@LB@@LB@Energy -12.83 kcal/mol@LB@Hybridization Energy -17.1909 kcal/mol@LB@Unfolding Energy - mRNA 4.30321 kcal/mol@LB@Unfolding Energy - sRNA 0.0608678 kcal/mol@LB@@LB@Position - mRNA 249 -- 268@LB@Position - sRNA 2 -- 18@LB@Position Seed - mRNA 262 -- 268@LB@Position Seed - sRNA 2 -- 8@LB@
132	0.0212	0.526	b2455	eutE	-9.17	0.140915	224 -- 235	1 -- 12	aldehyde oxidoreductase ethanolamine utilization protein	_____________223__________236\n_____________\|____________\|\n5'-GGU...GAACA________A___GGUAC...UCG-3'\n______________GGUGGUGA_AGC\n______________::\|\|:\|\|\|_\|\|\|\n______________UUACUACU_UCG\n3'-UUU...UUCAA________A___-5'\n_____________\|___________\|\n_____________13__________1\n	225 -- 231	5 -- 11	-12.5804 kcal/mol	3.3751 kcal/mol	0.0394463 kcal/mol	223 236@LB@ \| \|@LB@5'-GGU...GAACA A GGUAC...UCG-3'@LB@ GGUGGUGA AGC@LB@ ::\|\|:\|\|\| \|\|\|@LB@ UUACUACU UCG@LB@3'-UUU...UUCAA A -5'@LB@ \| \|@LB@ 13 1@LB@@LB@@LB@Energy -9.17 kcal/mol@LB@Hybridization Energy -12.5804 kcal/mol@LB@Unfolding Energy - mRNA 3.3751 kcal/mol@LB@Unfolding Energy - sRNA 0.0394463 kcal/mol@LB@@LB@Position - mRNA 224 -- 235@LB@Position - sRNA 1 -- 12@LB@Position Seed - mRNA 225 -- 231@LB@Position Seed - sRNA 5 -- 11@LB@
133	0.02154	0.526	b1105	lpoB	-10.17	0.089721	187 -- 196	50 -- 59	OM lipoprotein stimulator of MrcB transpeptidase	_____________186________197\n_____________\|__________\|\n5'-UUA...UUGUA__________CUUGA...AAC-3'\n______________AGGGGUGAAU\n______________\|\|\|\|\|\|:\|\|:\n______________UCCCCAUUUG\n3'-UUU...CUUUA__________UCUGU...UCG-5'\n_____________\|__________\|\n_____________60_________49\n	190 -- 196	50 -- 56	-15.1396 kcal/mol	4.24097 kcal/mol	0.728772 kcal/mol	186 197@LB@ \| \|@LB@5'-UUA...UUGUA CUUGA...AAC-3'@LB@ AGGGGUGAAU@LB@ \|\|\|\|\|\|:\|\|:@LB@ UCCCCAUUUG@LB@3'-UUU...CUUUA UCUGU...UCG-5'@LB@ \| \|@LB@ 60 49@LB@@LB@@LB@Energy -10.17 kcal/mol@LB@Hybridization Energy -15.1396 kcal/mol@LB@Unfolding Energy - mRNA 4.24097 kcal/mol@LB@Unfolding Energy - sRNA 0.728772 kcal/mol@LB@@LB@Position - mRNA 187 -- 196@LB@Position - sRNA 50 -- 59@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 50 -- 56@LB@
134	0.02184	0.526	b0034	caiF	-11.03	0.059446	251 -- 259	1 -- 9	cai operon transcriptional activator	_____________250_______260\n_____________\|_________\|\n5'-UGA...GAAUG_________UGAAA...AUU-3'\n______________GAUGAUGGC\n______________\|\|\|\|\|\|:\|\|\n______________CUACUAUCG\n3'-UUU...AAUUA_________-5'\n_____________\|________\|\n_____________10_______1\n	253 -- 259	1 -- 7	-13.9707 kcal/mol	2.91573 kcal/mol	0.0200155 kcal/mol	250 260@LB@ \| \|@LB@5'-UGA...GAAUG UGAAA...AUU-3'@LB@ GAUGAUGGC@LB@ \|\|\|\|\|\|:\|\|@LB@ CUACUAUCG@LB@3'-UUU...AAUUA -5'@LB@ \| \|@LB@ 10 1@LB@@LB@@LB@Energy -11.03 kcal/mol@LB@Hybridization Energy -13.9707 kcal/mol@LB@Unfolding Energy - mRNA 2.91573 kcal/mol@LB@Unfolding Energy - sRNA 0.0200155 kcal/mol@LB@@LB@Position - mRNA 251 -- 259@LB@Position - sRNA 1 -- 9@LB@Position Seed - mRNA 253 -- 259@LB@Position Seed - sRNA 1 -- 7@LB@
135	0.02191	0.526	b1342	ydaN	-17.19	0.001818	129 -- 197	1 -- 58	putative Zn(II) transporter	_____________128______________________________________________________________________198\n_____________\|________________________________________________________________________\|\n5'-CGU...GUAAU_______UG_______GCGUAACAGGGC______GCUA____UACA________UGC___________A___ACUGU...AUG-3'\n______________GGGGUGA__AGAUGUU____________CAGAAG_____GAC______AAAUAA___GUUGAUGAUGG_GGC\n______________\|\|\|\|\|:\|__\|\|\|::\|\|____________\|\|\|\|\|:_____\|\|\|______\|\|\|\|\|\|___\|\|\|:\|\|\|\|\|\|:_:\|\|\n______________CCCCAUU__UCUGUAA____________GUCUUU_____CUG______UUUAUU___CAAUUACUACU_UCG\n3'-UUU...UUUAU_______UG_________________________ACUUA___CCAUUA______U_____________A___-5'\n_____________\|_______________________________________________________________________\|\n_____________59______________________________________________________________________1\n	187 -- 193	5 -- 11	-40.4273 kcal/mol	16.9018 kcal/mol	6.33758 kcal/mol	128 198@LB@ \| \|@LB@5'-CGU...GUAAU UG GCGUAACAGGGC GCUA UACA UGC A ACUGU...AUG-3'@LB@ GGGGUGA AGAUGUU CAGAAG GAC AAAUAA GUUGAUGAUGG GGC@LB@ \|\|\|\|\|:\| \|\|\|::\|\| \|\|\|\|\|: \|\|\| \|\|\|\|\|\| \|\|\|:\|\|\|\|\|\|: :\|\|@LB@ CCCCAUU UCUGUAA GUCUUU CUG UUUAUU CAAUUACUACU UCG@LB@3'-UUU...UUUAU UG ACUUA CCAUUA U A -5'@LB@ \| \|@LB@ 59 1@LB@@LB@@LB@Energy -17.19 kcal/mol@LB@Hybridization Energy -40.4273 kcal/mol@LB@Unfolding Energy - mRNA 16.9018 kcal/mol@LB@Unfolding Energy - sRNA 6.33758 kcal/mol@LB@@LB@Position - mRNA 129 -- 197@LB@Position - sRNA 1 -- 58@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 5 -- 11@LB@
136	0.02238	0.526	b1883	cheB	-10.46	0.078180	185 -- 206	1 -- 23	fused chemotaxis regulator: protein-glutamate methylesterase in two-component regulatory system with CheA	_____________184______________________207\n_____________\|________________________\|\n5'-GGG...UGCGC_________A____C_________AAAAU...CGA-3'\n______________UAAGUAAGG_UUAA_GAUGA__GC\n______________\|\|\|:\|\|\|:\|_\|\|\|\|_\|\|\|\|\|__\|\|\n______________AUUUAUUUC_AAUU_CUACU__CG\n3'-UUU...CCAUU______________A_____AU__-5'\n_____________\|_______________________\|\n_____________24______________________1\n	187 -- 193	15 -- 21	-15.1436 kcal/mol	4.57462 kcal/mol	0.105066 kcal/mol	184 207@LB@ \| \|@LB@5'-GGG...UGCGC A C AAAAU...CGA-3'@LB@ UAAGUAAGG UUAA GAUGA GC@LB@ \|\|\|:\|\|\|:\| \|\|\|\| \|\|\|\|\| \|\|@LB@ AUUUAUUUC AAUU CUACU CG@LB@3'-UUU...CCAUU A AU -5'@LB@ \| \|@LB@ 24 1@LB@@LB@@LB@Energy -10.46 kcal/mol@LB@Hybridization Energy -15.1436 kcal/mol@LB@Unfolding Energy - mRNA 4.57462 kcal/mol@LB@Unfolding Energy - sRNA 0.105066 kcal/mol@LB@@LB@Position - mRNA 185 -- 206@LB@Position - sRNA 1 -- 23@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 15 -- 21@LB@
137	0.02279	0.526	b1739	osmE	-8.84	0.162198	202 -- 246	21 -- 65	osmotically-inducible lipoprotein	_____________201____________________________________________247\n_____________\|______________________________________________\|\n5'-GAA...GAAUA____CA__AAUAU______A____U______C__C_______U___CCAUG...AGC-3'\n______________UGAA__AG_____GGCAGG_AUUC__GAGUG_AG_GGCGGUA_UAA\n______________\|\|\|\|__\|\|_____::\|\|\|:_\|\|\|\|__:\|:\|\|_\|:_\|:\|\|\|\|\|_\|\|\|\n______________ACUU__UC_____UUGUCU_UAAG__UUUAC_UU_CUGCCAU_AUU\n3'-UUU...GGCCA____UA__CCCAU______G____UC________A_______U___UAUUU...UCG-5'\n_____________\|______________________________________________\|\n_____________66_____________________________________________20\n	236 -- 242	25 -- 31	-25.0909 kcal/mol	9.03477 kcal/mol	7.21767 kcal/mol	201 247@LB@ \| \|@LB@5'-GAA...GAAUA CA AAUAU A U C C U CCAUG...AGC-3'@LB@ UGAA AG GGCAGG AUUC GAGUG AG GGCGGUA UAA@LB@ \|\|\|\| \|\| ::\|\|\|: \|\|\|\| :\|:\|\| \|: \|:\|\|\|\|\| \|\|\|@LB@ ACUU UC UUGUCU UAAG UUUAC UU CUGCCAU AUU@LB@3'-UUU...GGCCA UA CCCAU G UC A U UAUUU...UCG-5'@LB@ \| \|@LB@ 66 20@LB@@LB@@LB@Energy -8.84 kcal/mol@LB@Hybridization Energy -25.0909 kcal/mol@LB@Unfolding Energy - mRNA 9.03477 kcal/mol@LB@Unfolding Energy - sRNA 7.21767 kcal/mol@LB@@LB@Position - mRNA 202 -- 246@LB@Position - sRNA 21 -- 65@LB@Position Seed - mRNA 236 -- 242@LB@Position Seed - sRNA 25 -- 31@LB@
138	0.02287	0.526	b0081	mraZ	-12.90	0.022954	180 -- 193	52 -- 64	RsmH methytransferase inhibitor	_____________179____________194\n_____________\|______________\|\n5'-GAU...AAGUG_____A________GGCUG...AAA-3'\n______________GGAAU_AGGGGUGA\n______________\|:\|\|\|_\|\|\|\|\|\|:\|\n______________CUUUA_UCCCCAUU\n3'-UUU...GCCAA______________UGUCU...UCG-5'\n_____________\|______________\|\n_____________65_____________51\n	187 -- 193	52 -- 58	-14.7021 kcal/mol	1.72638 kcal/mol	0.0778991 kcal/mol	179 194@LB@ \| \|@LB@5'-GAU...AAGUG A GGCUG...AAA-3'@LB@ GGAAU AGGGGUGA@LB@ \|:\|\|\| \|\|\|\|\|\|:\|@LB@ CUUUA UCCCCAUU@LB@3'-UUU...GCCAA UGUCU...UCG-5'@LB@ \| \|@LB@ 65 51@LB@@LB@@LB@Energy -12.90 kcal/mol@LB@Hybridization Energy -14.7021 kcal/mol@LB@Unfolding Energy - mRNA 1.72638 kcal/mol@LB@Unfolding Energy - sRNA 0.0778991 kcal/mol@LB@@LB@Position - mRNA 180 -- 193@LB@Position - sRNA 52 -- 64@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 52 -- 58@LB@
139	0.0229	0.526	b3958	argC	-10.25	0.086580	11 -- 36	23 -- 56	N-acetyl-gamma-glutamylphosphate reductase NAD(P)-binding	_____________10___________________________________37\n_____________\|____________________________________\|\n5'-AUC...AGCGC_______CUC__________________________UUGUU...CUG-3'\n______________GGUAAAU___GAUA__________AAUGGCGGUAAU\n______________\|\|\|\|\|\|:___\|\|:\|__________\|\|\|\|:\|\|\|\|\|\|\|\n______________CCAUUUG___CUGU__________UUACUGCCAUUA\n3'-UUU...UAUCC_______U______AAGUCUUUAC____________UUUAU...UCG-5'\n_____________\|____________________________________\|\n_____________57___________________________________22\n	30 -- 36	23 -- 29	-21.8632 kcal/mol	5.30467 kcal/mol	6.31216 kcal/mol	10 37@LB@ \| \|@LB@5'-AUC...AGCGC CUC UUGUU...CUG-3'@LB@ GGUAAAU GAUA AAUGGCGGUAAU@LB@ \|\|\|\|\|\|: \|\|:\| \|\|\|\|:\|\|\|\|\|\|\|@LB@ CCAUUUG CUGU UUACUGCCAUUA@LB@3'-UUU...UAUCC U AAGUCUUUAC UUUAU...UCG-5'@LB@ \| \|@LB@ 57 22@LB@@LB@@LB@Energy -10.25 kcal/mol@LB@Hybridization Energy -21.8632 kcal/mol@LB@Unfolding Energy - mRNA 5.30467 kcal/mol@LB@Unfolding Energy - sRNA 6.31216 kcal/mol@LB@@LB@Position - mRNA 11 -- 36@LB@Position - sRNA 23 -- 56@LB@Position Seed - mRNA 30 -- 36@LB@Position Seed - sRNA 23 -- 29@LB@
140	0.02319	0.526	b3615	waaH	-10.58	0.073933	178 -- 212	1 -- 47	LPS(HepIII)-glucuronic acid glycosyltransferase	_____________177______________________________________________213\n_____________\|________________________________________________\|\n5'-GAU...GGAUA____________C____U_______UU________________AC___ACCAA...GGA-3'\n______________AC_UUCAGAGGU____G_CGGUAAU___________AUGAUGA__AGC\n______________\|\|_\|\|\|\|\|\|::\|____\|_\|\|\|\|\|\|\|___________\|\|\|\|\|\|\|__\|\|\|\n______________UG_AAGUCUUUA____C_GCCAUUA___________UACUACU__UCG\n3'-UUU...UUGUC__U_________CUUA_U_______UUUAUUUCAAU_______A____-5'\n_____________\|_______________________________________________\|\n_____________48______________________________________________1\n	201 -- 207	5 -- 11	-24.9941 kcal/mol	8.09222 kcal/mol	6.32011 kcal/mol	177 213@LB@ \| \|@LB@5'-GAU...GGAUA C U UU AC ACCAA...GGA-3'@LB@ AC UUCAGAGGU G CGGUAAU AUGAUGA AGC@LB@ \|\| \|\|\|\|\|\|::\| \| \|\|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|@LB@ UG AAGUCUUUA C GCCAUUA UACUACU UCG@LB@3'-UUU...UUGUC U CUUA U UUUAUUUCAAU A -5'@LB@ \| \|@LB@ 48 1@LB@@LB@@LB@Energy -10.58 kcal/mol@LB@Hybridization Energy -24.9941 kcal/mol@LB@Unfolding Energy - mRNA 8.09222 kcal/mol@LB@Unfolding Energy - sRNA 6.32011 kcal/mol@LB@@LB@Position - mRNA 178 -- 212@LB@Position - sRNA 1 -- 47@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 5 -- 11@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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