Input and runtime details for job 3751556 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_000913
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 3751556 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:09:18 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:12:06 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:12:59 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:14:52 CET 2020
	Job Completed		@ Mon Jan 27 14:15:02 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3751556&toolName=IntaRNA ( 30 days expiry )

Description of the job

CyaR - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
b2859	-59	-36	-46	-40	6.61	CyaR	9	32	13	19	1.48	-12.89	-20.98	-1.36526	CAGCGGGCUUGUAAUGGGUUAAGU&ACAUAACCCAUAAAAUGCUAGCUG	((((..((.....((((((((.((&)).)))))))).....))..))))	ACGGGAAUAUCUGCGGCAGCGGGCUUGUAAUGGGUUAAGUGAUAACAGAUGUCUGGAAAUAUAGGGGCAAAUCCAAUAAACAAUUGGAAUUUACUUCCAUUGAUCUGUUUACUCAGUGGUUGUCACUUUUAUCGAGAAAGGUUUGCAGAA	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0091552	0.9277422	ygeO	947325	pseudogene orgA family part of T3SS PAI ETT2 remnant
b3600	54	72	57	63	6.76	CyaR	14	33	24	30	1.44	-10.46	-18.66	-1.10788	ACUGCUGGCAGACGCGGGU&ACCCAUAAAAUGCUAGCUGU	((.(((((((.....((((&))))......))))))).))	CACGGAGAGGGUUUGGGUGAGGGAAAAGCCUCACCCCAGCCCUCUCGGGUAAAAACAUUGAUGAAGGUUAAUACUAUGAAAGCAUUACAUUUUGGCGCAGGUAAUAUCGGUCGUGGCUUUAUCGGUAAACUGCUGGCAGACGCGGGUAUC	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0378997	0.9277422	mtlD	948117	mannitol-1-phosphate dehydrogenase NAD-dependent
b3423	37	59	51	57	6.83	CyaR	9	38	11	17	4.53	-12.11	-23.47	-1.28264	CUGGUUAAACAGCAGGGUUAUGU&ACAUAACCCAUAAAAUGCUAGCUGUACCAG	(((((...(((((.(((((((((&)))))))))...........))))))))))	UAGCCGUGGGUUUAGCGAUGGCUUUUGUUGAUUCGCUCAAUGCGCGAAAACGAAAAUAAUUCCAGGGAUUUAUAAAUGAAACAAACACAACGUCACAACGGUAUUAUCGAACUGGUUAAACAGCAGGGUUAUGUCAGUACCGAAGAGCUG	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0147559	0.9277422	glpR	947926	pseudogene DNA-binding transcriptional repressorregulator Energy metabolism carbon: Anaerobic respiration repressor of the glp operon
b2667	-64	-42	-48	-42	7.3	CyaR	20	48	20	26	4.56	-10.49	-22.35	-1.11106	GAGGCUGGUAUAUGUUGCAUUUU&AAAAUGCUAGCUGUACCAGGAACCACCUC	((((((((((((.((((((((((&))))))).)))))))))))......))))	UUUACGCUGCUGAGGCUGGUAUAUGUUGCAUUUUUAGUUUAGAGUUUACUUAAUUUAGAAAAUACUUAAAUAAAUAUGACUGAACUCGCGCAAUUACAGGCCAGUGCCGAACAGGCAGCGGCCUUAUUGAAAGCAAUGAGCCACCCUAAA	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0372843	0.9277422	ygaV	947136	tributyltin-inducible repressor of ygaVP
b2721	-73	-43	-49	-43	7.31	CyaR	1	39	1	7	4.6	-11.06	-22.97	-1.17143	CCUGGGCUGGGUUUGGCUUUGCAUUUUUAGC&GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGG	((((((((((...(((.((....((((((((&))))))))....)).))).......)))))....)))))	UACCUGGGCUGGGUUUGGCUUUGCAUUUUUAGCGUUCGUCUCCUUGCUGGCGGCGUGAUUAAAGAGAGUUUGAGCAUGUCUGAAGAAAAAUUAGGUCAACAUUAUCUCGCCGCGCUGAAUGAGGCAUUUCCGGGCGUCGUGCUGGACCAC	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0271691	0.9277422	hycE	947396	hydrogenase 3 large subunit
b2025	47	72	47	53	7.32	CyaR	25	47	41	47	4.55	-10.83	-22.7	-1.14707	AGGUGGUGAAAGGCGUACAGUUUCGC&GCUAGCUGUACCAGGAACCACCU	(((((((.......((((((((..((&)).)))))))).....)))))))	UGGCACUGGUGUGCGCGGCGUAAUAGUUGGUCGGGCAUUACUGGAAGGUAAAUUCACCGUGAAGGAGGCCAUCGCAUGCUGGCAAAACGCAUAAUCCCAUGUCUCGACGUUCGUGAUGGUCAGGUGGUGAAAGGCGUACAGUUUCGCAAC	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0309086	0.9277422	hisF	946516	imidazole glycerol phosphate synthase catalytic subunit with HisH
b4523	-72	-41	-64	-58	7.48	CyaR	15	49	35	41	4.57	-11.84	-23.89	-1.25404	GGCGGUUUUUCUUGGUUCUGCACUUUUCUGGG&CCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCC	((.(((..((((((((...((..((((.((((&)))).)))).....))...))))))))..))).))	GCUGGCGGUUUUUCUUGGUUCUGCACUUUUCUGGGUGGUUGUCGCACUGCUGAUUUGGAAAGUGUGGGGAUAACUAUGGUCGGUCAGGAGCAACUGGAGUCGUCACCAUUAUGCCAGCAUAGUGACAAUGAAACAGAAACGAAACGGGAA	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0173235	0.9277422	yciX	1450257	uncharacterized protein
b2568	40	60	54	60	7.67	CyaR	16	38	16	22	4.49	-12.94	-25.1	-1.37055	CUGGUGACGGGCAUUUUAUGG&CCAUAAAAUGCUAGCUGUACCAG	(((((.(((((((((((((((&)))))))))))...)))))))))	GUCGAGCUGCCGCAGGAAGCGUUCCUCGCCAUUCUGCACGUCGGCAAAGACAACAAAUAACCCUUAGGAGUUGGCAUGGCGAAUAUGUUUGCCCUGAUUCUGGUGAUUGCCACACUGGUGACGGGCAUUUUAUGGUGCGUGGAUAAAUUC	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0088729	0.9277422	lepB	947040	leader peptidase (signal peptidase I)
b2957	43	68	53	59	7.84	CyaR	22	43	29	35	4.55	-12.83	-25.22	-1.3589	GGUUUUAGUGGUGCAGCAUUGGCAUU&AAUGCUAGCUGUACCAGGAACC	((((....(((((((((..(((((((&))))))))))))))))..))))	UAAAUUGUUUAACGUCAAAUUUCCCAUACAGAGCUAAGGGAUAAUGCGUAGCGUUCACGUAACUGGAGGAAUGAAAUGGAGUUUUUCAAAAAGACGGCACUUGCCGCACUGGUUAUGGGUUUUAGUGGUGCAGCAUUGGCAUUACCCAAU	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0095047	0.9277422	ansB	947454	periplasmic L-asparaginase 2
b1278	-56	-30	-56	-50	7.85	CyaR	10	41	35	41	4.6	-12.73	-25.18	-1.34831	UUCCUGGAACUGGCGUUUUCAGUUAUG&CAUAACCCAUAAAAUGCUAGCUGUACCAGGAA	(((((((..((((((((((..((((((&))))))....)))))))))).....)))))))	GGCACAAAUCUGUCAAUUUUUCCUGGAACUGGCGUUUUCAGUUAUGAUUGUGGGACUUAUCAAAAAGGAGAGGCCAUGCGUUCGAUUGCCAGACGUACCGCAGUGGGAGCUGCACUAUUGCUUGUCAUGCCAGUAGCCGUAUGGAUUUCU	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0101143	0.9277422	pgpB	945863	phosphatidylglycerophosphatase B

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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