Input and runtime details for job 3751556 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_000913
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 3751556 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:09:18 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:12:06 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:12:59 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:14:52 CET 2020
	Job Completed		@ Mon Jan 27 14:15:02 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3751556&toolName=IntaRNA ( 30 days expiry )

Description of the job

CyaR - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
b4686	-70	-59	-70	-64	1.79	CyaR	19	30	24	30	1.38	-11.09	-14.26	-1.17461	GCUGGUAUUUUA&UAAAAUGCUAGC	((((((((((((&))))))))))))	AAAUUGCUGGUAUUUUACGCUUUACUGUUCCGAUAAGUUCAGUAUGAUCUUGCCCGGAAAACGGGGAGAGUCAUUAUGCUGGAAUCAAUAAUUAAUCUGGUAUCGAGUGGCGCAGUUGACAGCCACACACCGCAAACUGCCGUUGCUGCC	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0267126	0.9277422	yshB	7751621	uncharacterized protein
b4342	-53	-28	-45	-39	2.01	CyaR	5	28	15	21	1.43	-12.97	-16.41	-1.37373	UAGAAAUGUUGUGGGGUUUUGUUUUU&AAAAACAUAACCCAUAAAAUGCUA	(((.....(((((((....(((((((&)))))))...)))))))....)))	UCUGAAAGGUUUUCUUUCAAAUUAGAAAUGUUGUGGGGUUUUGUUUUUGUAUCUUUUAUCUCUAAGGAGCUUGCUUUGGGUCAAUCAGAAUACAUUUCAUGGGUAAAAUGUACUUCCUGGCUAAGUAACUUUGUGAAUCUUAGAGGGUUG	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0087074	0.9277422	yjiT	945056	pseudogene
b2636	-57	-40	-55	-49	2.03	CyaR	9	23	15	21	0.17	-11.73	-13.93	-1.24239	UUUUGUGGGAAUUUAUGU&ACAUAACCCAUAAAA	(((((((((...((((((&)))))))))))))))	CGGCGUGCCGGUGGGUAUUUUUGUGGGAAUUUAUGUCUUUGCCAAAGUGGUUCGUCUGAUUUCAGGGAAAAAAUAAUGAAACGUAAAACACUACCUCUGCUGGCGCUGGUUGCCACCUCUCUGUUUCUGAGCGCCUGCGAUGACAGGAGU	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0184807	0.9277422	yfjS	945668	CP4-57 prophage uncharacterized protein
b1615	-74	-64	-74	-68	2.11	CyaR	24	35	29	35	1.42	-12.16	-15.69	-1.28794	GUGCAGCAGCA&UGCUAGCUGUAC	(((((((((((&)))).)))))))	AGUGCAGCAGCAAUUAAUCAGCGAUAUCACUAAUUAAUAUUCAAUAAAAAUAAUCAGAACAUCAAAGGUGCAACUAUGAGAAAAAUAGUGGCCAUGGCCGUUAUUUGCCUGACGGCUGCCUCUGGCCUUACCUCUGCUUAUGCGGCGCAA	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0143201	0.9277422	uidC	944820	putative outer membrane porin for beta-glucuronides porin protein
b4040	30	39	31	37	2.12	CyaR	21	30	23	29	1.38	-10.32	-13.82	-1.09305	GCUGGCGUUU&AAAUGCUAGC	((((((((((&))))))))))	CGAUUAAGCGGUAAACCGCUGUUGCUAACAGAACUGUUUUUACCGGCGUCACCGUUGUACUAAGAGGAAAAAAAUAUGGAGUGGAGUCUGACGCAGAAUAAGCUGCUGGCGUUUCAUCGCUUAAUGCGUACGGAUAAGCCAAUUGGCGCG	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0408932	0.9277422	ubiA	948540	p-hydroxybenzoate octaprenyltransferase
b2939	64	73	64	70	2.17	CyaR	11	20	14	20	0.16	-10.76	-13.09	-1.13966	UAUGGGUUGU&AUAACCCAUA	((((((((((&))))))))))	CUUGUAAAAACAAGAGUUUUCUCGUGGUUUCGCCGAACUUUCACACUUACGUUCGGUUAUGUGCUUAAUAAUGUUAUGAAAAAGAAACCGGUUGCGCAGUUGGAGCGUCAGCAUUCACUGCUGGAAAAUCCAUGUGCUUAUGGGUUGUUA	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.032135	0.9277422	yqgB	947433	uncharacterized protein
b3890	60	67	61	67	2.18	CyaR	29	36	29	35	1.16	-10.64	-13.98	-1.12695	GGUGCAGC&GCUGUACC	((((((((&))))))))	CAAGGGUACUUUUCUCUCCAUAGAUUCAAAAAUGAUAUUGGCGAGAUAUUUAUGAACUCUCUGGCAGGUAUAGAUAUGGGCAGAAUUUUACUCGAUUUAUCGAAUGAGGUGAUUAAGCAACUUGAUGAUCUCGAGGUGCAGCGUAAUCUU	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0343392	0.9277422	yiiF	948385	putative thymol sensitivity protein CopG family putative transcriptional regulator
b4547	23	35	29	35	2.33	CyaR	34	46	34	40	3.2	-13.97	-19.5	-1.47965	GGUGGAUUCUGGU&ACCAGGAACCACC	(((((.(((((((&))))))).)))))	CUACUUGCCCGUAUGUAUCAACGUAUCAUGGAACAGCUCGUCGCCUGAUGAGUGGUUCUGCAAGAGGAAAUAAGCAUGGACUGGCUGGCUAAAUAUUGGUGGAUUCUGGUGAUUGUCUUUUUGGUAGGCGUCCUGCUGAACGUGAUUAAA	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.004561	0.8345133	ypfN	1450286	putative membrane protein UPF0370 family
b3284	-60	-47	-59	-53	2.73	CyaR	16	31	24	30	1.42	-10.6	-14.75	-1.12271	AGUUAGCAGGAUGG&CCAUAAAAUGCUAGCU	((((((((..((((&))))....))))))))	AACUACUCGAACUGGAGUUAGCAGGAUGGAUCGCAGCUGUACCCGGCGGCUAUGUCCGAUUGAGGAGGGCAUGCCAUGUUCGACGUACUAAUGUAUUUGUUUGAAACCUAUAUUCACACAGAAGCUGAGUUGCGUGUGGAUCAAGACAAA	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0351035	0.9277422	smg	947781	DUF494 family putative periplasmic protein
b0435	-63	-44	-55	-49	2.84	CyaR	21	36	26	32	1.43	-13.8	-18.07	-1.46164	GGUAAAAGCGGCUAGUAUUU&AAAUGCUAGCUGUACC	((((....((((((((((((&))))))))))))))))	UGCAAUGGAAACGGUAAAAGCGGCUAGUAUUUAAAGGGAUGGAUGACAUCUCAGCGUUGUCGGAGGAGAUAUUUCAUGAUGAUACGUGAGCGGAUAGAAGAAAAAUUAAGGGCGGCGUUCCAACCCGUAUUCCUCGAAGUAGUGGAUGAA	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0051044	0.8345133	bolA	947043	stationary-phase morphogene transcriptional repressor for mreB also regulator for dacA dacC and ampC

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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