Input and runtime details for job 8403881 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_000913
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 8403881 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:15:25 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:18:18 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:19:13 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:20:45 CET 2020
	Job Completed		@ Mon Jan 27 14:20:57 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=8403881&toolName=IntaRNA ( 30 days expiry )

Description of the job

RyhB - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
b2819	20	33	23	29	4.07	RyhB	47	62	53	59	3.09	-12.43	-19.59	-1.30698	UACUGGAAGCUGUG&CACAUUGCUUCCAGUA	((((((((((((((&))))..))))))))))	CACAACCCGACCCAACGCCGGGUUGAUUGCCCUGAUGGAUGAGAUGUUUGCCGGUAUGACCCUGGAGGAGGCGUAAUGAAAUUGCAAAAGCAAUUACUGGAAGCUGUGGAGCACAAACAGCUACGCCCGCUGGAUGUGCAAUUUGCCCUG	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0130826	0.9496255	recD	947287	exonuclease V (RecBCD complex) alpha chain
b2155	-55	-41	-50	-44	3.08	RyhB	41	57	44	50	3.2	-11.36	-17.64	-1.19448	GAAGAUGUGAGCGAU&AUUGCUCACAUUGCUUC	(((((((((((((((&)))))))))))..))))	CGUACGCAAGGGGACGUGAAGAAGAUGUGAGCGAUAACCCAUUUUAUUUUCGUAGUUACCUCAUGGAGAUAUGGAAUGUUUAGGUUGAACCCUUUCGUACGGGUCGGGCUGUGUUUGUCCGCUAUUUCUUGUGCAUGGCCUGUGUUAGCG	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0231967	0.9496255	cirA	949042	colicin IA outer membrane receptor and translocator ferric iron-catecholate transporter
b3607	-11	7	-2	5	4.66	RyhB	36	59	38	44	3.29	-13.23	-21.18	-1.3911	UGGAGUAAGCAAUGUCGU&ACGACAUUGCUCACAUUGCUUCCA	((((((((((((((((((&))))))))))).....))).))))	AGGGAACCUUUGUUACCGCUAUGACCCGGCCCGCGCAGAACGGGCCGGUCAUUAUCUCAUCGUGUGGAGUAAGCAAUGUCGUGUGAAGAACUGGAAAUUGUCUGGAACAAUAUUAAAGCCGAAGCCAGAACGCUGGCGGACUGUGAGCCA	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0084199	0.9291058	cysE	948126	serine acetyltransferase
b4348	-73	-53	-59	-53	7.54	RyhB	40	66	40	46	3.3	-15.87	-26.71	-1.66869	GUGAACUGGGGGCGAGCGAUG&CAUUGCUCACAUUGCUUCCAGUAUUAC	(((((((((((((((((((((&)))))))).....))))))))).))))	GCGUGAACUGGGGGCGAGCGAUGAGGCCGAUUUGCAGCGUCAGUUGCUGGAAGAAGCGUUUGGUGGGGUGAAGGAAUGAGUGCGGGGAAAUUGCCGGAGGGGUGGGUUAUCGCCCCAGUAUCUACGGUCACAACUCUAAUCCGAGGAGUA	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0018209	0.7909534	hsdS	948867	specificity determinant for hsdM and hsdR
b2239	58	75	58	64	6.18	RyhB	37	58	52	58	3.22	-11.5	-20.9	-1.2092	GGAAGCAGUGCAAUGGCG&CGACAUUGCUCACAUUGCUUCC	(((((((((((((((.((&)).))))))....)))))))))	UGUUGAUUGUUGUGAUGAUUGGCGAAAAACGUCGCCAUGAACAAUUACUGCAAGAACGCAACGGAGGCUAAUGGCAUGAAAUUGACGCUGAAAAACCUUAGCAUGGCGAUCAUGAUGAGCACUAUAGUCAUGGGAAGCAGUGCAAUGGCG	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0215456	0.9496255	glpQ	946725	periplasmic glycerophosphodiester phosphodiesterase
b1683	8	33	27	33	4.83	RyhB	38	66	38	44	3.3	-21.41	-29.54	-2.25121	GUAAUACUGAAGCAACUGACGAUGUC&GACAUUGCUCACAUUGCUUCCAGUAUUAC	(((((((((((((((.((((((((((&))))))).)))..)))))).)))))))))	GAUAUUCAAAUUUCACAACCCUAAAGCCCAGAAUGAAUGUGGCUGUGGCGAAAGCUUUGGGGUAUAGGCGGUACUAUGUCUCGUAAUACUGAAGCAACUGACGAUGUCAAAACCUGGACCGGCGGCCCGCUGAAUUAUAAAGAAGGAUUC	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	4.71e-05	0.1636725	sufB	945753	component of SufBCD Fe-S cluster assembly scaffold
b3010	-68	-29	-35	-29	12.31	RyhB	38	86	38	44	14.91	-11.97	-39.19	-1.25862	GCCAGCGCCCUGGCUCUCAAUGCUCAUUUCUGCCAAUGUC&GACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGC	(((((((((((((((...((((((((.......(((((((&))))))).......)).....))))))....)))))...))))))))))	UGUGAUCGCCAGCGCCCUGGCUCUCAAUGCUCAUUUCUGCCAAUGUCUUGCCUAUUUCUCCAGAGUGCUGGAGAAAUGCUACAAAAUUGCGCACAAUCAAAUUGCCGCAUUAUUCCUAAGAAAUUACGCGAUAUGAAACGUGAAGAGAUU	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0167745	0.9496255	yqhC	947491	transcriptional activator of yqhD
b0032	-16	-1	-16	-10	5.23	RyhB	10	26	20	26	4.37	-11	-20.6	-1.15662	UUCUCUGGAGGGUGUU&AAGACCCUCGCGGAGAA	(((((((((((((.((&)).)))))).)))))))	AGAUUUGCAUUGAUUUACGUCAUCAUUGUGAAUUAAUAUGCAAAUAAAGUGAGUGAAUAUUCUCUGGAGGGUGUUUUGAUUAAGUCAGCGCUAUUGGUUCUGGAAGACGGAACCCAGUUUCACGGUCGGGCCAUAGGGGCAACAGGUUCG	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0280038	0.9496255	carA	949025	carbamoyl phosphate synthetase small subunit glutamine amidotransferase
b0722	-33	-15	-32	-26	4.14	RyhB	22	41	34	40	6.69	-14.16	-24.99	-1.48889	UGUCGUGCUUUCACUUCUC&GAGAACCUGAAAGCACGACA	(((((((((((((..((((&))))...)))))))))))))	CGAAGCGGGUAAACGCUCCGCCAAAAUCUCCUUUGUUAUUACUGUCGUGCUUUCACUUCUCGCAGGAGUCCUCGUAUGGUAAGCAACGCCUCCGCAUUAGGACGCAAUGGCGUACAUGAUUUCAUCCUCGUUCGCGCUACCGCUAUCGUC	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0049771	0.9291058	sdhD	945322	succinate dehydrogenase membrane subunit binds cytochrome b556
b4532	-9	18	-5	2	7.78	RyhB	38	58	48	54	3.21	-13.67	-24.66	-1.43737	GGAGGCGAUGUGAAACAAAGCGAGUUC&GACAUUGCUCACAUUGCUUCC	(((((((((((((......((((..((&))..)))))))))))))))))	AUUUUCGAGCAAAUUACACACGGAGGUAAACACUAAUGUUUACUUUGUUGAUAUACUCAGCGGCAGGGAGGCGAUGUGAAACAAAGCGAGUUCAGACGUUGGCUCGAAUCUCAGGGCGUCGAUGUAGCGAAUGGCAGCAACCAUUUGAAA	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0065776	0.9291058	hicA	945989	mRNA interferase toxin of the HicAB toxin-antitoxin system

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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