Input and runtime details for job 6756176 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6756176 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:22:58 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:23:17 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:01:55 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:02:03 CET 2018
	Job Completed		@ Fri Feb 16 20:02:30 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6756176&toolName=CopraRNA ( 30 days expiry )

Description of the job

DsrA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
61	0.003184	0.1645	b4112	basS	-6.79	0.124428	7 -- 41	10 -- 54	sensory histidine kinase in two-component regulatory system with BasR		_____________6______________________________________________42\n_____________\|______________________________________________\|\n_____5'-GGUUAA____CA_______C___C_____________GU_____________CUCUA...UAU-3'\n______________UGCU__AAGCAGG_AGU____________CG__GCAUCGGGAAAUU\n______________\|\|\|\|__\|\|\|\|\|:\|_\|\|:____________\|\|__:\|\|:\|:\|\|\|\|\|\|:\n______________ACGA__UUCGUUC_UCG____________GC__UGUGGUCCUUUAG\n3'-UUU...UUUGA____A________U___UGAAUUUUUUAA__AA_____________ACUAC...CAA-5'\n_____________\|______________________________________________\|\n_____________55_____________________________________________9\n	35 -- 41	10 -- 16	-31.3926 kcal/mol	10.7631 kcal/mol	13.8372 kcal/mol	6 42@LB@ \| \|@LB@ 5'-GGUUAA CA C C GU CUCUA...UAU-3'@LB@ UGCU AAGCAGG AGU CG GCAUCGGGAAAUU@LB@ \|\|\|\| \|\|\|\|\|:\| \|\|: \|\| :\|\|:\|:\|\|\|\|\|\|:@LB@ ACGA UUCGUUC UCG GC UGUGGUCCUUUAG@LB@3'-UUU...UUUGA A U UGAAUUUUUUAA AA ACUAC...CAA-5'@LB@ \| \|@LB@ 55 9@LB@@LB@@LB@Energy -6.79 kcal/mol@LB@Hybridization Energy -31.3926 kcal/mol@LB@Unfolding Energy - mRNA 10.7631 kcal/mol@LB@Unfolding Energy - sRNA 13.8372 kcal/mol@LB@@LB@Position - mRNA 7 -- 41@LB@Position - sRNA 10 -- 54@LB@Position Seed - mRNA 35 -- 41@LB@Position Seed - sRNA 10 -- 16@LB@
62	0.00332	0.1645	b1305	pspB	-5.29	0.278747	189 -- 201	34 -- 46	psp operon transcription co-activator		_____________188___________202\n_____________\|_____________\|\n5'-AUU...UCGUC_____________UGAGC...GUG-3'\n______________UAAGGAGUACUUA\n______________:\|\|\|:\|\|:\|\|\|\|\|\n______________GUUCUUCGUGAAU\n3'-UUU...AAUUC_____________UUUUU...CAA-5'\n_____________\|_____________\|\n_____________47____________33\n	195 -- 201	34 -- 40	-14.7021 kcal/mol	3.41245 kcal/mol	5.99897 kcal/mol	188 202@LB@ \| \|@LB@5'-AUU...UCGUC UGAGC...GUG-3'@LB@ UAAGGAGUACUUA@LB@ :\|\|\|:\|\|:\|\|\|\|\|@LB@ GUUCUUCGUGAAU@LB@3'-UUU...AAUUC UUUUU...CAA-5'@LB@ \| \|@LB@ 47 33@LB@@LB@@LB@Energy -5.29 kcal/mol@LB@Hybridization Energy -14.7021 kcal/mol@LB@Unfolding Energy - mRNA 3.41245 kcal/mol@LB@Unfolding Energy - sRNA 5.99897 kcal/mol@LB@@LB@Position - mRNA 189 -- 201@LB@Position - sRNA 34 -- 46@LB@Position Seed - mRNA 195 -- 201@LB@Position Seed - sRNA 34 -- 40@LB@
63	0.003338	0.1645	b2671	ygaC	-8.88	0.034356	231 -- 246	25 -- 40	uncharacterized protein		_____________230______________247\n_____________\|________________\|\n5'-UAU...CGCGC____________G___GUUUU...AAA-3'\n______________GUACUUGAAAAA_UCG\n______________\|:\|\|\|\|:\|\|\|\|\|_\|\|\|\n______________CGUGAAUUUUUU_AGC\n3'-UUU...UUCUU____________A___AAUGU...CAA-5'\n_____________\|________________\|\n_____________41_______________24\n	231 -- 237	34 -- 40	-15.9773 kcal/mol	5.44668 kcal/mol	1.64821 kcal/mol	230 247@LB@ \| \|@LB@5'-UAU...CGCGC G GUUUU...AAA-3'@LB@ GUACUUGAAAAA UCG@LB@ \|:\|\|\|\|:\|\|\|\|\| \|\|\|@LB@ CGUGAAUUUUUU AGC@LB@3'-UUU...UUCUU A AAUGU...CAA-5'@LB@ \| \|@LB@ 41 24@LB@@LB@@LB@Energy -8.88 kcal/mol@LB@Hybridization Energy -15.9773 kcal/mol@LB@Unfolding Energy - mRNA 5.44668 kcal/mol@LB@Unfolding Energy - sRNA 1.64821 kcal/mol@LB@@LB@Position - mRNA 231 -- 246@LB@Position - sRNA 25 -- 40@LB@Position Seed - mRNA 231 -- 237@LB@Position Seed - sRNA 34 -- 40@LB@
64	0.003339	0.1645	b3166	truB	-7.27	0.093914	187 -- 199	36 -- 48	tRNA pseudouridine synthase B: tRNA pseudouridine(55) synthase and putative tmRNA pseudouridine(342) synthase		_____________186___________200\n_____________\|_____________\|\n5'-GGC...ACGAC_________G___AAUGA...AAC-3'\n______________AGCAAGGAG_ACU\n______________\|\|\|\|\|\|:\|\|_\|\|\|\n______________UCGUUCUUC_UGA\n3'-UUU...CGAAU_________G___AUUUU...CAA-5'\n_____________\|_____________\|\n_____________49____________35\n	189 -- 195	40 -- 46	-16.8762 kcal/mol	3.76451 kcal/mol	5.83861 kcal/mol	186 200@LB@ \| \|@LB@5'-GGC...ACGAC G AAUGA...AAC-3'@LB@ AGCAAGGAG ACU@LB@ \|\|\|\|\|\|:\|\| \|\|\|@LB@ UCGUUCUUC UGA@LB@3'-UUU...CGAAU G AUUUU...CAA-5'@LB@ \| \|@LB@ 49 35@LB@@LB@@LB@Energy -7.27 kcal/mol@LB@Hybridization Energy -16.8762 kcal/mol@LB@Unfolding Energy - mRNA 3.76451 kcal/mol@LB@Unfolding Energy - sRNA 5.83861 kcal/mol@LB@@LB@Position - mRNA 187 -- 199@LB@Position - sRNA 36 -- 48@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 40 -- 46@LB@
65	0.003382	0.1645	b1739	osmE	-4.35	0.428849	173 -- 228	7 -- 58	osmotically-inducible lipoprotein		_____________172______________________________________________________229\n_____________\|________________________________________________________\|\n5'-GAA...UAUCU___AC___GA___A_______A__GAAUAUGAACAAGAA____G______U_____GUGCA...AGC-3'\n______________AAC__GCU__UAA_CAAGAGG_CG_______________UAUG_CAGGAA_UCUGA\n______________\|\|\|__\|\|\|__\|\|\|_\|\|\|\|\|:\|_\|:_______________\|\|::_\|\|\|\|\|\|_\|\|\|\|\|\n______________UUG__CGA__AUU_GUUCUUC_GU_______________AUGU_GUCCUU_AGACU\n3'-UUU...CUACU___AA________C__________GAAUUUUUUAAGCA_____G______U_____ACACAA-5'\n_____________\|________________________________________________________\|\n_____________59_______________________________________________________6\n	187 -- 193	40 -- 46	-28.7812 kcal/mol	10.5809 kcal/mol	13.8467 kcal/mol	172 229@LB@ \| \|@LB@5'-GAA...UAUCU AC GA A A GAAUAUGAACAAGAA G U GUGCA...AGC-3'@LB@ AAC GCU UAA CAAGAGG CG UAUG CAGGAA UCUGA@LB@ \|\|\| \|\|\| \|\|\| \|\|\|\|\|:\| \|: \|\|:: \|\|\|\|\|\| \|\|\|\|\|@LB@ UUG CGA AUU GUUCUUC GU AUGU GUCCUU AGACU@LB@3'-UUU...CUACU AA C GAAUUUUUUAAGCA G U ACACAA-5'@LB@ \| \|@LB@ 59 6@LB@@LB@@LB@Energy -4.35 kcal/mol@LB@Hybridization Energy -28.7812 kcal/mol@LB@Unfolding Energy - mRNA 10.5809 kcal/mol@LB@Unfolding Energy - sRNA 13.8467 kcal/mol@LB@@LB@Position - mRNA 173 -- 228@LB@Position - sRNA 7 -- 58@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 40 -- 46@LB@
66	0.003432	0.1645	b0882	clpA	-7.45	0.084488	282 -- 290	34 -- 42	ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease chaperone activity		_____________281_______291\n_____________\|_________\|\n5'-UUU...CCGUC_________UUACU...CGC-3'\n______________GAGCACUUG\n______________:\|\|\|\|\|\|\|:\n______________UUCGUGAAU\n3'-UUU...CGUUC_________UUUUU...CAA-5'\n_____________\|_________\|\n_____________43________33\n	284 -- 290	34 -- 40	-10.5586 kcal/mol	1.49281 kcal/mol	1.61613 kcal/mol	281 291@LB@ \| \|@LB@5'-UUU...CCGUC UUACU...CGC-3'@LB@ GAGCACUUG@LB@ :\|\|\|\|\|\|\|:@LB@ UUCGUGAAU@LB@3'-UUU...CGUUC UUUUU...CAA-5'@LB@ \| \|@LB@ 43 33@LB@@LB@@LB@Energy -7.45 kcal/mol@LB@Hybridization Energy -10.5586 kcal/mol@LB@Unfolding Energy - mRNA 1.49281 kcal/mol@LB@Unfolding Energy - sRNA 1.61613 kcal/mol@LB@@LB@Position - mRNA 282 -- 290@LB@Position - sRNA 34 -- 42@LB@Position Seed - mRNA 284 -- 290@LB@Position Seed - sRNA 34 -- 40@LB@
67	0.003475	0.1645	b0416	nusB	-6.41	0.154688	140 -- 154	27 -- 40	transcription antitermination protein		_____________139_____________155\n_____________\|_______________\|\n5'-AAG...UGACC__________U____AAUGU...AGU-3'\n______________GCGCUUGAAA_GAUU\n______________\|\|:\|\|\|:\|\|\|_:\|\|\|\n______________CGUGAAUUUU_UUAA\n3'-UUU...UUCUU_______________GCAAU...CAA-5'\n_____________\|_______________\|\n_____________41______________26\n	142 -- 148	32 -- 38	-12.213 kcal/mol	4.16264 kcal/mol	1.6425 kcal/mol	139 155@LB@ \| \|@LB@5'-AAG...UGACC U AAUGU...AGU-3'@LB@ GCGCUUGAAA GAUU@LB@ \|\|:\|\|\|:\|\|\| :\|\|\|@LB@ CGUGAAUUUU UUAA@LB@3'-UUU...UUCUU GCAAU...CAA-5'@LB@ \| \|@LB@ 41 26@LB@@LB@@LB@Energy -6.41 kcal/mol@LB@Hybridization Energy -12.213 kcal/mol@LB@Unfolding Energy - mRNA 4.16264 kcal/mol@LB@Unfolding Energy - sRNA 1.6425 kcal/mol@LB@@LB@Position - mRNA 140 -- 154@LB@Position - sRNA 27 -- 40@LB@Position Seed - mRNA 142 -- 148@LB@Position Seed - sRNA 32 -- 38@LB@
68	0.003533	0.1645	b3650	spoT	-9.27	0.026581	116 -- 140	36 -- 60	bifunctional (p)ppGpp synthetase II/ guanosine-3' 5'-bis pyrophosphate 3'-pyrophosphohydrolase		_____________115________________________141\n_____________\|__________________________\|\n5'-UAG...CGCCA_____A___AA___________C___GAAUU...CUC-3'\n______________GGAAC__GC__GAGCAGGAAGC_GCU\n______________\|:\|\|\|__\|\|__:\|\|\|\|:\|\|\|\|\|_:\|\|\n______________CUUUG__CG__UUCGUUCUUCG_UGA\n3'-UUU...CCCUA_____AA__AA_______________AUUUU...CAA-5'\n_____________\|__________________________\|\n_____________61_________________________35\n	130 -- 136	39 -- 45	-24.6059 kcal/mol	9.08429 kcal/mol	6.24973 kcal/mol	115 141@LB@ \| \|@LB@5'-UAG...CGCCA A AA C GAAUU...CUC-3'@LB@ GGAAC GC GAGCAGGAAGC GCU@LB@ \|:\|\|\| \|\| :\|\|\|\|:\|\|\|\|\| :\|\|@LB@ CUUUG CG UUCGUUCUUCG UGA@LB@3'-UUU...CCCUA AA AA AUUUU...CAA-5'@LB@ \| \|@LB@ 61 35@LB@@LB@@LB@Energy -9.27 kcal/mol@LB@Hybridization Energy -24.6059 kcal/mol@LB@Unfolding Energy - mRNA 9.08429 kcal/mol@LB@Unfolding Energy - sRNA 6.24973 kcal/mol@LB@@LB@Position - mRNA 116 -- 140@LB@Position - sRNA 36 -- 60@LB@Position Seed - mRNA 130 -- 136@LB@Position Seed - sRNA 39 -- 45@LB@
69	0.00357	0.1645	b0571	cusR	-5.90	0.203942	86 -- 99	6 -- 21	response regulator in two-component regulatory system with CusS	b1969	_____________85_______________100\n_____________\|________________\|\n5'-AGC...UUACU_______C________UUUCC...GGC-3'\n______________CGCCAGG___UCUGAU\n______________\|:\|\|\|\|\|___\|\|\|\|\|\|\n______________GUGGUCC___AGACUA\n3'-UUU...GCAAU_______UUU______CACAA-5'\n_____________\|________________\|\n_____________22_______________5\n	86 -- 92	15 -- 21	-18.4182 kcal/mol	4.95815 kcal/mol	7.56001 kcal/mol	85 100@LB@ \| \|@LB@5'-AGC...UUACU C UUUCC...GGC-3'@LB@ CGCCAGG UCUGAU@LB@ \|:\|\|\|\|\| \|\|\|\|\|\|@LB@ GUGGUCC AGACUA@LB@3'-UUU...GCAAU UUU CACAA-5'@LB@ \| \|@LB@ 22 5@LB@@LB@@LB@Energy -5.90 kcal/mol@LB@Hybridization Energy -18.4182 kcal/mol@LB@Unfolding Energy - mRNA 4.95815 kcal/mol@LB@Unfolding Energy - sRNA 7.56001 kcal/mol@LB@@LB@Position - mRNA 86 -- 99@LB@Position - sRNA 6 -- 21@LB@Position Seed - mRNA 86 -- 92@LB@Position Seed - sRNA 15 -- 21@LB@
70	0.003574	0.1645	b0145	dksA	-6.64	0.135564	191 -- 197	39 -- 45	transcriptional regulator of rRNA transcription; DnaK suppressor protein		_____________190_____198\n_____________\|_______\|\n5'-AAU...UUAAG_______AACAU...AUG-3'\n______________GAGAAGC\n______________:\|\|\|\|\|\|\n______________UUCUUCG\n3'-UUU...AUUCG_______UGAAU...CAA-5'\n_____________\|_______\|\n_____________46______38\n	191 -- 197	39 -- 45	-7.63285 kcal/mol	0.475666 kcal/mol	0.514773 kcal/mol	190 198@LB@ \| \|@LB@5'-AAU...UUAAG AACAU...AUG-3'@LB@ GAGAAGC@LB@ :\|\|\|\|\|\|@LB@ UUCUUCG@LB@3'-UUU...AUUCG UGAAU...CAA-5'@LB@ \| \|@LB@ 46 38@LB@@LB@@LB@Energy -6.64 kcal/mol@LB@Hybridization Energy -7.63285 kcal/mol@LB@Unfolding Energy - mRNA 0.475666 kcal/mol@LB@Unfolding Energy - sRNA 0.514773 kcal/mol@LB@@LB@Position - mRNA 191 -- 197@LB@Position - sRNA 39 -- 45@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 39 -- 45@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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