Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
181	0.01309	0.2504	b2799	fucO	-15.36	0.022654	183 -- 205	1 -- 21	L-1 2-propanediol oxidoreductase	_____________182_____________________206\n_____________\|_______________________\|\n5'-CUU...GUAAA___A__________G_U_A____UAACA...CGC-3'\n______________GCA_CAAGGAGAAG_A_G_UGGC\n______________:\|\|_\|\|\|:\|\|:\|\|\|_\|_\|_\|\|\|\|\n______________UGU_GUUUCUUUUC_U_C_ACCG\n3'-UUU...ACCCC___A__________G________-5'\n_____________\|______________________\|\n_____________22_____________________1\n	190 -- 196	8 -- 14	-20.3858 kcal/mol	3.51699 kcal/mol	1.51103 kcal/mol	182 206@LB@ \| \|@LB@5'-CUU...GUAAA A G U A UAACA...CGC-3'@LB@ GCA CAAGGAGAAG A G UGGC@LB@ :\|\| \|\|\|:\|\|:\|\|\| \| \| \|\|\|\|@LB@ UGU GUUUCUUUUC U C ACCG@LB@3'-UUU...ACCCC A G -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -15.36 kcal/mol@LB@Hybridization Energy -20.3858 kcal/mol@LB@Unfolding Energy - mRNA 3.51699 kcal/mol@LB@Unfolding Energy - sRNA 1.51103 kcal/mol@LB@@LB@Position - mRNA 183 -- 205@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 8 -- 14@LB@
182	0.01317	0.2504	b2101	yegW	-15.40	0.022099	126 -- 162	3 -- 28	putative DNA-binding transcriptional regulator	_____________125______________________________________163\n_____________\|________________________________________\|\n5'-CGG...UCGUU_________________AACGCCUUAAAUAA_________AUAUA...AAA-3'\n______________GAUGGG___AUUAAAGA______________GAAGCAGUG\n______________:\|\|\|\|\|___\|\|:\|\|\|\|\|______________:\|\|\|\|\|\|\|\|\n______________UUACCC___UAGUUUCU______________UUUCGUCAC\n3'-UUU...GUGUU______CUG_______________________________CG-5'\n_____________\|________________________________________\|\n_____________29_______________________________________2\n	156 -- 162	3 -- 9	-24.3736 kcal/mol	6.45453 kcal/mol	2.51813 kcal/mol	125 163@LB@ \| \|@LB@5'-CGG...UCGUU AACGCCUUAAAUAA AUAUA...AAA-3'@LB@ GAUGGG AUUAAAGA GAAGCAGUG@LB@ :\|\|\|\|\| \|\|:\|\|\|\|\| :\|\|\|\|\|\|\|\|@LB@ UUACCC UAGUUUCU UUUCGUCAC@LB@3'-UUU...GUGUU CUG CG-5'@LB@ \| \|@LB@ 29 2@LB@@LB@@LB@Energy -15.40 kcal/mol@LB@Hybridization Energy -24.3736 kcal/mol@LB@Unfolding Energy - mRNA 6.45453 kcal/mol@LB@Unfolding Energy - sRNA 2.51813 kcal/mol@LB@@LB@Position - mRNA 126 -- 162@LB@Position - sRNA 3 -- 28@LB@Position Seed - mRNA 156 -- 162@LB@Position Seed - sRNA 3 -- 9@LB@
183	0.01318	0.2504	b4483	tatD	-12.95	0.080004	100 -- 117	8 -- 25	quality control of Tat-exported FeS proteins; Mg-dependent cytoplasmic DNase	_____________99__________________118\n_____________\|___________________\|\n5'-CUG...GUAAA___C__A____________GAAAA...UCA-3'\n______________GGG_GA_AUC_GGGAAGAG\n______________\|\|\|_\|\|_\|\|\|_::\|\|\|:\|\|\n______________CCC_CU_UAG_UUCUUUUC\n3'-UUU...UUUUA______G___U________GUCACCG-5'\n_____________\|___________________\|\n_____________26__________________7\n	111 -- 117	8 -- 14	-16.1661 kcal/mol	2.51597 kcal/mol	0.697717 kcal/mol	99 118@LB@ \| \|@LB@5'-CUG...GUAAA C A GAAAA...UCA-3'@LB@ GGG GA AUC GGGAAGAG@LB@ \|\|\| \|\| \|\|\| ::\|\|\|:\|\|@LB@ CCC CU UAG UUCUUUUC@LB@3'-UUU...UUUUA G U GUCACCG-5'@LB@ \| \|@LB@ 26 7@LB@@LB@@LB@Energy -12.95 kcal/mol@LB@Hybridization Energy -16.1661 kcal/mol@LB@Unfolding Energy - mRNA 2.51597 kcal/mol@LB@Unfolding Energy - sRNA 0.697717 kcal/mol@LB@@LB@Position - mRNA 100 -- 117@LB@Position - sRNA 8 -- 25@LB@Position Seed - mRNA 111 -- 117@LB@Position Seed - sRNA 8 -- 14@LB@
184	0.0132	0.2504	b2897	sdhE	-10.91	0.194803	203 -- 220	7 -- 23	flavinator of succinate dehydrogenase; antitoxin of CptAB toxin-antitoxin pair	_____________202________________221\n_____________\|__________________\|\n5'-GUA...AAGAU_________CAAC_____CCGCA...ACA-3'\n______________GGACAUUAA____AAAGC\n______________\|\|\|\|\|\|:\|\|____\|\|\|\|\|\n______________CCUGUAGUU____UUUCG\n3'-UUU...UUACC_________UCU______UCACCG-5'\n_____________\|__________________\|\n_____________24_________________6\n	205 -- 211	15 -- 21	-14.6885 kcal/mol	3.17611 kcal/mol	0.599063 kcal/mol	202 221@LB@ \| \|@LB@5'-GUA...AAGAU CAAC CCGCA...ACA-3'@LB@ GGACAUUAA AAAGC@LB@ \|\|\|\|\|\|:\|\| \|\|\|\|\|@LB@ CCUGUAGUU UUUCG@LB@3'-UUU...UUACC UCU UCACCG-5'@LB@ \| \|@LB@ 24 6@LB@@LB@@LB@Energy -10.91 kcal/mol@LB@Hybridization Energy -14.6885 kcal/mol@LB@Unfolding Energy - mRNA 3.17611 kcal/mol@LB@Unfolding Energy - sRNA 0.599063 kcal/mol@LB@@LB@Position - mRNA 203 -- 220@LB@Position - sRNA 7 -- 23@LB@Position Seed - mRNA 205 -- 211@LB@Position Seed - sRNA 15 -- 21@LB@
185	0.0132	0.2504	b2009	sbmC	-15.75	0.018057	263 -- 294	1 -- 27	DNA gyrase inhibitor	_____________262______________________________295\n_____________\|________________________________\|\n5'-CUG...GGCCC_____AACA____G___________UU__A__AGUUGA-3'\n______________GUGGG____GACG_UAAAGAAAGGC__UG_GC\n______________:\|\|\|\|____\|\|\|:_:\|\|\|\|\|\|\|:\|\|__\|\|_\|\|\n______________UACCC____CUGU_GUUUCUUUUCG__AC_CG\n3'-UUU...UGUUU_____________A___________UC_____-5'\n_____________\|_______________________________\|\n_____________28______________________________1\n	281 -- 287	7 -- 13	-27.1244 kcal/mol	8.85955 kcal/mol	2.51867 kcal/mol	262 295@LB@ \| \|@LB@5'-CUG...GGCCC AACA G UU A AGUUGA-3'@LB@ GUGGG GACG UAAAGAAAGGC UG GC@LB@ :\|\|\|\| \|\|\|: :\|\|\|\|\|\|\|:\|\| \|\| \|\|@LB@ UACCC CUGU GUUUCUUUUCG AC CG@LB@3'-UUU...UGUUU A UC -5'@LB@ \| \|@LB@ 28 1@LB@@LB@@LB@Energy -15.75 kcal/mol@LB@Hybridization Energy -27.1244 kcal/mol@LB@Unfolding Energy - mRNA 8.85955 kcal/mol@LB@Unfolding Energy - sRNA 2.51867 kcal/mol@LB@@LB@Position - mRNA 263 -- 294@LB@Position - sRNA 1 -- 27@LB@Position Seed - mRNA 281 -- 287@LB@Position Seed - sRNA 7 -- 13@LB@
186	0.01333	0.2514	b0084	ftsI	-11.80	0.135121	196 -- 214	1 -- 22	transpeptidase involved in septal peptidoglycan synthesis; penicillin-binding protein 3	_____________195____________________215\n_____________\|______________________\|\n5'-GUU...AACGC_______U__________C___GAAAA...UGG-3'\n______________GACG_CA___GAAAGCAG_GGC\n______________\|\|\|:_\|\|___:\|\|\|\|\|\|\|_\|\|\|\n______________CUGU_GU___UUUUCGUC_CCG\n3'-UUU...UACCC____A__UUC________A___-5'\n_____________\|_____________________\|\n_____________23____________________1\n	204 -- 210	5 -- 11	-21.3841 kcal/mol	8.04628 kcal/mol	1.54023 kcal/mol	195 215@LB@ \| \|@LB@5'-GUU...AACGC U C GAAAA...UGG-3'@LB@ GACG CA GAAAGCAG GGC@LB@ \|\|\|: \|\| :\|\|\|\|\|\|\| \|\|\|@LB@ CUGU GU UUUUCGUC CCG@LB@3'-UUU...UACCC A UUC A -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -11.80 kcal/mol@LB@Hybridization Energy -21.3841 kcal/mol@LB@Unfolding Energy - mRNA 8.04628 kcal/mol@LB@Unfolding Energy - sRNA 1.54023 kcal/mol@LB@@LB@Position - mRNA 196 -- 214@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 204 -- 210@LB@Position Seed - sRNA 5 -- 11@LB@
187	0.01374	0.2529	b1344	ttcA	-15.51	0.020761	46 -- 73	1 -- 29	tRNA s(2)C32 thioltransferase iron sulfur cluster protein	_____________45_____________________________74\n_____________\|______________________________\|\n5'-GCU...CAGCC____________G___CUGC_____A____GUAUG...UCA-3'\n______________AAAUGGGG_CAU_AAA_____GCAG_UGGU\n______________\|\|\|\|\|\|\|\|_\|\|\|_\|\|\|_____\|\|\|\|_\|\|\|:\n______________UUUACCCC_GUA_UUU_____CGUC_ACCG\n3'-UUU...GGUGU________U___G___CUUUU_________-5'\n_____________\|_____________________________\|\n_____________30____________________________1\n	47 -- 53	22 -- 28	-26.0543 kcal/mol	8.02666 kcal/mol	2.51907 kcal/mol	45 74@LB@ \| \|@LB@5'-GCU...CAGCC G CUGC A GUAUG...UCA-3'@LB@ AAAUGGGG CAU AAA GCAG UGGU@LB@ \|\|\|\|\|\|\|\| \|\|\| \|\|\| \|\|\|\| \|\|\|:@LB@ UUUACCCC GUA UUU CGUC ACCG@LB@3'-UUU...GGUGU U G CUUUU -5'@LB@ \| \|@LB@ 30 1@LB@@LB@@LB@Energy -15.51 kcal/mol@LB@Hybridization Energy -26.0543 kcal/mol@LB@Unfolding Energy - mRNA 8.02666 kcal/mol@LB@Unfolding Energy - sRNA 2.51907 kcal/mol@LB@@LB@Position - mRNA 46 -- 73@LB@Position - sRNA 1 -- 29@LB@Position Seed - mRNA 47 -- 53@LB@Position Seed - sRNA 22 -- 28@LB@
188	0.01388	0.2529	b4198	ulaF	-10.93	0.193396	24 -- 37	2 -- 17	L-ribulose 5-phosphate 4-epimerase	_____________23_______________38\n_____________\|________________\|\n5'-GUC...UUUGG______U_________AUUUC...GCG-3'\n______________CGAAGG___GUAGUGG\n______________\|:\|\|\|:___\|:\|\|\|\|\|\n______________GUUUCU___CGUCACC\n3'-UUU...CUGUA______UUU_______G-5'\n_____________\|________________\|\n_____________18_______________1\n	31 -- 37	2 -- 8	-15.3961 kcal/mol	3.02615 kcal/mol	1.43836 kcal/mol	23 38@LB@ \| \|@LB@5'-GUC...UUUGG U AUUUC...GCG-3'@LB@ CGAAGG GUAGUGG@LB@ \|:\|\|\|: \|:\|\|\|\|\|@LB@ GUUUCU CGUCACC@LB@3'-UUU...CUGUA UUU G-5'@LB@ \| \|@LB@ 18 1@LB@@LB@@LB@Energy -10.93 kcal/mol@LB@Hybridization Energy -15.3961 kcal/mol@LB@Unfolding Energy - mRNA 3.02615 kcal/mol@LB@Unfolding Energy - sRNA 1.43836 kcal/mol@LB@@LB@Position - mRNA 24 -- 37@LB@Position - sRNA 2 -- 17@LB@Position Seed - mRNA 31 -- 37@LB@Position Seed - sRNA 2 -- 8@LB@
189	0.01393	0.2529	b0934	ssuC	-14.25	0.041816	137 -- 153	1 -- 17	aliphatic sulfonate ABC transporter permease	_____________136_______________154\n_____________\|_________________\|\n5'-UGC...AUCCG_____C___________GAAUG...GGC-3'\n______________CAAGG_GAAGCGGUGGC\n______________\|\|\|:\|_:\|\|\|\|:\|\|\|\|\|\n______________GUUUC_UUUCGUCACCG\n3'-UUU...CUGUA_____U___________-5'\n_____________\|________________\|\n_____________18_______________1\n	147 -- 153	1 -- 7	-24.8998 kcal/mol	9.21593 kcal/mol	1.43853 kcal/mol	136 154@LB@ \| \|@LB@5'-UGC...AUCCG C GAAUG...GGC-3'@LB@ CAAGG GAAGCGGUGGC@LB@ \|\|\|:\| :\|\|\|\|:\|\|\|\|\|@LB@ GUUUC UUUCGUCACCG@LB@3'-UUU...CUGUA U -5'@LB@ \| \|@LB@ 18 1@LB@@LB@@LB@Energy -14.25 kcal/mol@LB@Hybridization Energy -24.8998 kcal/mol@LB@Unfolding Energy - mRNA 9.21593 kcal/mol@LB@Unfolding Energy - sRNA 1.43853 kcal/mol@LB@@LB@Position - mRNA 137 -- 153@LB@Position - sRNA 1 -- 17@LB@Position Seed - mRNA 147 -- 153@LB@Position Seed - sRNA 1 -- 7@LB@
190	0.01401	0.2529	b2478	dapA	-10.08	0.266807	73 -- 98	6 -- 25	dihydrodipicolinate synthase	_____________72_________________________99\n_____________\|__________________________\|\n5'-GGC...UUUAA____UGA____UUCUU__________UAAAA...CCA-3'\n______________GGGG___GUUG_____AAGGAAAGCA\n______________\|\|\|\|___:\|::_____\|\|\|:\|\|\|\|\|\|\n______________CCCC___UAGU_____UUCUUUUCGU\n3'-UUU...UUUUA____UG____________________CACCG-5'\n_____________\|__________________________\|\n_____________26_________________________5\n	92 -- 98	6 -- 12	-17.2074 kcal/mol	6.41889 kcal/mol	0.70636 kcal/mol	72 99@LB@ \| \|@LB@5'-GGC...UUUAA UGA UUCUU UAAAA...CCA-3'@LB@ GGGG GUUG AAGGAAAGCA@LB@ \|\|\|\| :\|:: \|\|\|:\|\|\|\|\|\|@LB@ CCCC UAGU UUCUUUUCGU@LB@3'-UUU...UUUUA UG CACCG-5'@LB@ \| \|@LB@ 26 5@LB@@LB@@LB@Energy -10.08 kcal/mol@LB@Hybridization Energy -17.2074 kcal/mol@LB@Unfolding Energy - mRNA 6.41889 kcal/mol@LB@Unfolding Energy - sRNA 0.70636 kcal/mol@LB@@LB@Position - mRNA 73 -- 98@LB@Position - sRNA 6 -- 25@LB@Position Seed - mRNA 92 -- 98@LB@Position Seed - sRNA 6 -- 12@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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