Input and runtime details for job 6756176 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6756176 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:22:58 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:23:17 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:01:55 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:02:03 CET 2018
	Job Completed		@ Fri Feb 16 20:02:30 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6756176&toolName=CopraRNA ( 30 days expiry )

Description of the job

DsrA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
131	0.01234	0.3033	b4139	aspA	-5.03	0.315570	237 -- 252	26 -- 41	aspartate ammonia-lyase	_____________236______________253\n_____________\|________________\|\n5'-GAU...UGUUG_____CA_________CAGCU...ACU-3'\n______________GGUAC__GGGAAGUUC\n______________:\|:\|\|__:::\|\|:\|\|\|\n______________UCGUG__UUUUUUAAG\n3'-UUU...GUUCU_____AA_________CAAUG...CAA-5'\n_____________\|________________\|\n_____________42_______________25\n	246 -- 252	26 -- 32	-10.8102 kcal/mol	4.127 kcal/mol	1.64829 kcal/mol	236 253@LB@ \| \|@LB@5'-GAU...UGUUG CA CAGCU...ACU-3'@LB@ GGUAC GGGAAGUUC@LB@ :\|:\|\| :::\|\|:\|\|\|@LB@ UCGUG UUUUUUAAG@LB@3'-UUU...GUUCU AA CAAUG...CAA-5'@LB@ \| \|@LB@ 42 25@LB@@LB@@LB@Energy -5.03 kcal/mol@LB@Hybridization Energy -10.8102 kcal/mol@LB@Unfolding Energy - mRNA 4.127 kcal/mol@LB@Unfolding Energy - sRNA 1.64829 kcal/mol@LB@@LB@Position - mRNA 237 -- 252@LB@Position - sRNA 26 -- 41@LB@Position Seed - mRNA 246 -- 252@LB@Position Seed - sRNA 26 -- 32@LB@
132	0.01257	0.3067	b2516	rodZ	-8.98	0.032240	231 -- 240	34 -- 43	MreB assembly cytoskeletal protein	_____________230________241\n_____________\|__________\|\n5'-UAA...AAAAU__________CUACC...UUG-3'\n______________GAAGCACUUA\n______________\|\|\|\|\|\|\|\|\|\|\n______________CUUCGUGAAU\n3'-UUU...UCGUU__________UUUUU...CAA-5'\n_____________\|__________\|\n_____________44_________33\n	234 -- 240	34 -- 40	-13.7965 kcal/mol	1.10051 kcal/mol	3.71643 kcal/mol	230 241@LB@ \| \|@LB@5'-UAA...AAAAU CUACC...UUG-3'@LB@ GAAGCACUUA@LB@ \|\|\|\|\|\|\|\|\|\|@LB@ CUUCGUGAAU@LB@3'-UUU...UCGUU UUUUU...CAA-5'@LB@ \| \|@LB@ 44 33@LB@@LB@@LB@Energy -8.98 kcal/mol@LB@Hybridization Energy -13.7965 kcal/mol@LB@Unfolding Energy - mRNA 1.10051 kcal/mol@LB@Unfolding Energy - sRNA 3.71643 kcal/mol@LB@@LB@Position - mRNA 231 -- 240@LB@Position - sRNA 34 -- 43@LB@Position Seed - mRNA 234 -- 240@LB@Position Seed - sRNA 34 -- 40@LB@
133	0.0127	0.3077	b2452	eutH	-4.80	0.352106	186 -- 194	38 -- 46	ethanolamine transporter	_____________185_______195\n_____________\|_________\|\n5'-UUG...ACUAA_________AAGGC...UCC-3'\n______________CAGGGAGUA\n______________\|\|:\|:\|\|:\|\n______________GUUCUUCGU\n3'-UUU...AAUUC_________GAAUU...CAA-5'\n_____________\|_________\|\n_____________47________37\n	188 -- 194	38 -- 44	-8.62764 kcal/mol	1.2009 kcal/mol	2.62609 kcal/mol	185 195@LB@ \| \|@LB@5'-UUG...ACUAA AAGGC...UCC-3'@LB@ CAGGGAGUA@LB@ \|\|:\|:\|\|:\|@LB@ GUUCUUCGU@LB@3'-UUU...AAUUC GAAUU...CAA-5'@LB@ \| \|@LB@ 47 37@LB@@LB@@LB@Energy -4.80 kcal/mol@LB@Hybridization Energy -8.62764 kcal/mol@LB@Unfolding Energy - mRNA 1.2009 kcal/mol@LB@Unfolding Energy - sRNA 2.62609 kcal/mol@LB@@LB@Position - mRNA 186 -- 194@LB@Position - sRNA 38 -- 46@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 38 -- 44@LB@
134	0.01281	0.308	b3316	rpsS	-8.71	0.038435	274 -- 288	39 -- 53	30S ribosomal subunit protein S19	_____________273______________289\n_____________\|________________\|\n5'-GCG...GGAAA__G____A________CCCUG...CUU-3'\n______________GC__GAG_CAAGAAGC\n______________\|\|__:\|\|_\|\|\|\|\|\|\|\|\n______________CG__UUC_GUUCUUCG\n3'-UUU...UUGAA__AA____________UGAAU...CAA-5'\n_____________\|________________\|\n_____________54_______________38\n	282 -- 288	39 -- 45	-16.1876 kcal/mol	1.46049 kcal/mol	6.01745 kcal/mol	273 289@LB@ \| \|@LB@5'-GCG...GGAAA G A CCCUG...CUU-3'@LB@ GC GAG CAAGAAGC@LB@ \|\| :\|\| \|\|\|\|\|\|\|\|@LB@ CG UUC GUUCUUCG@LB@3'-UUU...UUGAA AA UGAAU...CAA-5'@LB@ \| \|@LB@ 54 38@LB@@LB@@LB@Energy -8.71 kcal/mol@LB@Hybridization Energy -16.1876 kcal/mol@LB@Unfolding Energy - mRNA 1.46049 kcal/mol@LB@Unfolding Energy - sRNA 6.01745 kcal/mol@LB@@LB@Position - mRNA 274 -- 288@LB@Position - sRNA 39 -- 53@LB@Position Seed - mRNA 282 -- 288@LB@Position Seed - sRNA 39 -- 45@LB@
135	0.01294	0.3087	b3785	wzzE	-4.46	0.409883	275 -- 291	39 -- 56	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein	_____________274_________________292\n_____________\|___________________\|\n5'-GCU...CGUAC___________U_______UAUGG...UUA-3'\n______________CUUGU__GGGC_GGGAAGC\n______________\|\|\|\|:__::\|\|_::\|\|\|\|\|\n______________GAACG__UUCG_UUCUUCG\n3'-UUU...ACUUU_____AA____________UGAAU...CAA-5'\n_____________\|___________________\|\n_____________57__________________38\n	285 -- 291	39 -- 45	-16.5835 kcal/mol	6.01236 kcal/mol	6.11167 kcal/mol	274 292@LB@ \| \|@LB@5'-GCU...CGUAC U UAUGG...UUA-3'@LB@ CUUGU GGGC GGGAAGC@LB@ \|\|\|\|: ::\|\| ::\|\|\|\|\|@LB@ GAACG UUCG UUCUUCG@LB@3'-UUU...ACUUU AA UGAAU...CAA-5'@LB@ \| \|@LB@ 57 38@LB@@LB@@LB@Energy -4.46 kcal/mol@LB@Hybridization Energy -16.5835 kcal/mol@LB@Unfolding Energy - mRNA 6.01236 kcal/mol@LB@Unfolding Energy - sRNA 6.11167 kcal/mol@LB@@LB@Position - mRNA 275 -- 291@LB@Position - sRNA 39 -- 56@LB@Position Seed - mRNA 285 -- 291@LB@Position Seed - sRNA 39 -- 45@LB@
136	0.01308	0.3087	b0131	panD	-6.12	0.181240	260 -- 282	17 -- 40	aspartate 1-decarboxylase	_____________259______________________283\n_____________\|________________________\|\n5'-AUU...GACCU_____AU_________________UUGAC...UUG-3'\n______________GCACU__GAAGGUUC_UUGCGCCA\n______________\|\|\|\|\|__:\|\|::\|\|\|_\|\|:\|:\|\|\|\n______________CGUGA__UUUUUAAG_AAUGUGGU\n3'-UUU...UUCUU_____AU________C________CCUUU...CAA-5'\n_____________\|________________________\|\n_____________41_______________________16\n	268 -- 274	26 -- 32	-23.1255 kcal/mol	7.80932 kcal/mol	9.19627 kcal/mol	259 283@LB@ \| \|@LB@5'-AUU...GACCU AU UUGAC...UUG-3'@LB@ GCACU GAAGGUUC UUGCGCCA@LB@ \|\|\|\|\| :\|\|::\|\|\| \|\|:\|:\|\|\|@LB@ CGUGA UUUUUAAG AAUGUGGU@LB@3'-UUU...UUCUU AU C CCUUU...CAA-5'@LB@ \| \|@LB@ 41 16@LB@@LB@@LB@Energy -6.12 kcal/mol@LB@Hybridization Energy -23.1255 kcal/mol@LB@Unfolding Energy - mRNA 7.80932 kcal/mol@LB@Unfolding Energy - sRNA 9.19627 kcal/mol@LB@@LB@Position - mRNA 260 -- 282@LB@Position - sRNA 17 -- 40@LB@Position Seed - mRNA 268 -- 274@LB@Position Seed - sRNA 26 -- 32@LB@
137	0.01315	0.3087	b2239	glpQ	-7.04	0.107729	242 -- 248	36 -- 42	periplasmic glycerophosphodiester phosphodiesterase	_____________241_____249\n_____________\|_______\|\n5'-CGG...AUGAU_______AUAGU...UCA-3'\n______________GAGCACU\n______________:\|\|\|\|\|\|\n______________UUCGUGA\n3'-UUU...CGUUC_______AUUUU...CAA-5'\n_____________\|_______\|\n_____________43______35\n	242 -- 248	36 -- 42	-9.09813 kcal/mol	0.455964 kcal/mol	1.60154 kcal/mol	241 249@LB@ \| \|@LB@5'-CGG...AUGAU AUAGU...UCA-3'@LB@ GAGCACU@LB@ :\|\|\|\|\|\|@LB@ UUCGUGA@LB@3'-UUU...CGUUC AUUUU...CAA-5'@LB@ \| \|@LB@ 43 35@LB@@LB@@LB@Energy -7.04 kcal/mol@LB@Hybridization Energy -9.09813 kcal/mol@LB@Unfolding Energy - mRNA 0.455964 kcal/mol@LB@Unfolding Energy - sRNA 1.60154 kcal/mol@LB@@LB@Position - mRNA 242 -- 248@LB@Position - sRNA 36 -- 42@LB@Position Seed - mRNA 242 -- 248@LB@Position Seed - sRNA 36 -- 42@LB@
138	0.01322	0.3087	b1523	yneG	-4.22	0.452951	267 -- 281	24 -- 41	DUF4186 family protein	_____________266________________282\n_____________\|__________________\|\n5'-AUG...GUCUG_____________G____CAGUA...AAG-3'\n______________GGCA__UGAAAGA__CGU\n______________:\|\|\|__\|:\|\|\|:\|__\|\|\|\n______________UCGU__AUUUUUU__GCA\n3'-UUU...GUUCU____GA_______AA___AUGUG...CAA-5'\n_____________\|__________________\|\n_____________42_________________23\n	271 -- 277	29 -- 35	-8.58312 kcal/mol	2.71204 kcal/mol	1.64881 kcal/mol	266 282@LB@ \| \|@LB@5'-AUG...GUCUG G CAGUA...AAG-3'@LB@ GGCA UGAAAGA CGU@LB@ :\|\|\| \|:\|\|\|:\| \|\|\|@LB@ UCGU AUUUUUU GCA@LB@3'-UUU...GUUCU GA AA AUGUG...CAA-5'@LB@ \| \|@LB@ 42 23@LB@@LB@@LB@Energy -4.22 kcal/mol@LB@Hybridization Energy -8.58312 kcal/mol@LB@Unfolding Energy - mRNA 2.71204 kcal/mol@LB@Unfolding Energy - sRNA 1.64881 kcal/mol@LB@@LB@Position - mRNA 267 -- 281@LB@Position - sRNA 24 -- 41@LB@Position Seed - mRNA 271 -- 277@LB@Position Seed - sRNA 29 -- 35@LB@
139	0.01338	0.3093	b3765	yifB	-8.91	0.033732	103 -- 139	26 -- 57	magnesium chelatase family protein and putative transcriptional regulator	_____________102______________________________________140\n_____________\|________________________________________\|\n5'-AAA...ACUUC______ACAAA___A_____AUC__C______________ACGAA...CGA-3'\n______________ACUUGC_____UAA_CAGGA___AG_CACU___AAAAUUC\n______________\|\|\|\|\|\|_____\|\|\|_\|\|:\|\|___\|\|_\|\|\|\|___\|\|\|\|\|\|\|\n______________UGAACG_____AUU_GUUCU___UC_GUGA___UUUUAAG\n3'-UUU...UACUU______A_______C_______________AUU_______CAAUG...CAA-5'\n_____________\|________________________________________\|\n_____________58_______________________________________25\n	133 -- 139	26 -- 32	-18.0428 kcal/mol	2.85625 kcal/mol	6.27605 kcal/mol	102 140@LB@ \| \|@LB@5'-AAA...ACUUC ACAAA A AUC C ACGAA...CGA-3'@LB@ ACUUGC UAA CAGGA AG CACU AAAAUUC@LB@ \|\|\|\|\|\| \|\|\| \|\|:\|\| \|\| \|\|\|\| \|\|\|\|\|\|\|@LB@ UGAACG AUU GUUCU UC GUGA UUUUAAG@LB@3'-UUU...UACUU A C AUU CAAUG...CAA-5'@LB@ \| \|@LB@ 58 25@LB@@LB@@LB@Energy -8.91 kcal/mol@LB@Hybridization Energy -18.0428 kcal/mol@LB@Unfolding Energy - mRNA 2.85625 kcal/mol@LB@Unfolding Energy - sRNA 6.27605 kcal/mol@LB@@LB@Position - mRNA 103 -- 139@LB@Position - sRNA 26 -- 57@LB@Position Seed - mRNA 133 -- 139@LB@Position Seed - sRNA 26 -- 32@LB@
140	0.01344	0.3093	b0426	yajQ	-3.92	0.510019	240 -- 246	37 -- 43	phage Phi6 host factor ATP/GTP binding protein	_____________239_____247\n_____________\|_______\|\n5'-UAA...UUCAG_______GUAAC...GUA-3'\n______________GAAGCAC\n______________\|\|\|\|\|\|\|\n______________CUUCGUG\n3'-UUU...UCGUU_______AAUUU...CAA-5'\n_____________\|_______\|\n_____________44______36\n	240 -- 246	37 -- 43	-10.1802 kcal/mol	2.59742 kcal/mol	3.6595 kcal/mol	239 247@LB@ \| \|@LB@5'-UAA...UUCAG GUAAC...GUA-3'@LB@ GAAGCAC@LB@ \|\|\|\|\|\|\|@LB@ CUUCGUG@LB@3'-UUU...UCGUU AAUUU...CAA-5'@LB@ \| \|@LB@ 44 36@LB@@LB@@LB@Energy -3.92 kcal/mol@LB@Hybridization Energy -10.1802 kcal/mol@LB@Unfolding Energy - mRNA 2.59742 kcal/mol@LB@Unfolding Energy - sRNA 3.6595 kcal/mol@LB@@LB@Position - mRNA 240 -- 246@LB@Position - sRNA 37 -- 43@LB@Position Seed - mRNA 240 -- 246@LB@Position Seed - sRNA 37 -- 43@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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