Input and runtime details for job 8403881 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_000913
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 8403881 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:15:25 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:18:18 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:19:13 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:20:45 CET 2020
	Job Completed		@ Mon Jan 27 14:20:57 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=8403881&toolName=IntaRNA ( 30 days expiry )

Description of the job

RyhB - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
b2830	62	74	68	74	1.12	RyhB	44	56	44	50	3.2	-13.5	-17.82	-1.41949	AGGUAAUGUGGGC&GCUCACAUUGCUU	(((((((((((((&)))))))))))))	GCAGAGAGUGGUUUACCGUCUUCCUGGAGUAUGAAACAAUCAUUCGUAUAUAAAGCUUUAUUUUGAGGUAGUCCGGUGAUUGAUGACGAUGGCUACCGCCCAAACGUAGGUAUCGUGAUUUGUAAUCGCCAGGGGCAGGUAAUGUGGGCC	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0072388	0.9291058	rppH	947300	RNA pyrophosphohydrolase
b1120	-17	5	-7	-1	9.07	RyhB	46	68	52	58	3.24	-14.88	-27.19	-1.5646	AAGUGGUGCCGGAAGCAAUGGA&UCACAUUGCUUCCAGUAUUACUU	(((((((((.((((((((((((&)).)))))))))).)))))))))	CGUAAAAAUAAACGCCGCCUGCGCGAGCGUUUGCGUCAGCGUAUUUUUUUCAGAGAUAAAGUGGUGCCGGAAGCAAUGGAAAAACCAAGAGUACUCGUACUGACAGGGGCAGGAAUUUCUGCGGAAUCAGGUAUUCGUACCUUUCGCGCC	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0032796	0.9291058	cobB	945687	deacetylase of acs and cheY chemotaxis regulator
b3391	-11	13	5	11	0.42	RyhB	46	68	49	55	3.24	-11.42	-15.08	-1.20079	AGGAGAUACAAAUGAAGCAAUGGA&UCACAUUGCUUCCAGUAUUACUU	(((..((((....(((((((((((&)).)))))))))..))))..)))	CAGACGUACUAACGCUGGGUACCGGGACAAACUGCGAACCGCCACAAUGGUUGUGGCAACGGCAAGGAGAUACAAAUGAAGCAAUGGAUAGCCGCACUACUGUUGAUGCUGAUACCCGGCGUACAGGCGGCAAAGCCGCAAAAAGUGACG	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0224751	0.9496255	hofQ	947901	DNA catabolic putative fimbrial transporter
b1279	-12	4	-9	-3	7.4	RyhB	46	61	52	58	3.24	-10.81	-21.45	-1.13665	ACGGGAAGUAAUGUGA&UCACAUUGCUUCCAGU	((.(((((((((((((&))))))))))))).))	AAAAUGGUACUUUAAAGGGCUAUUGCGGUAAGUUGACCAUAAUUUAUUCGCUCUAACCACAUAACGGGAAGUAAUGUGAAAUAUUUACUCAUUUUCUUACUGGUGUUAGCGAUCUUCGUGAUUUCGGUCACGUUGGGUGCGCAGAACGAU	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0309022	0.9496255	yciS	944936	DUF1049 family inner membrane protein function unknown
b2819	20	33	23	29	4.07	RyhB	47	62	53	59	3.09	-12.43	-19.59	-1.30698	UACUGGAAGCUGUG&CACAUUGCUUCCAGUA	((((((((((((((&))))..))))))))))	CACAACCCGACCCAACGCCGGGUUGAUUGCCCUGAUGGAUGAGAUGUUUGCCGGUAUGACCCUGGAGGAGGCGUAAUGAAAUUGCAAAAGCAAUUACUGGAAGCUGUGGAGCACAAACAGCUACGCCCGCUGGAUGUGCAAUUUGCCCUG	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0130826	0.9496255	recD	947287	exonuclease V (RecBCD complex) alpha chain
b1189	10	30	13	19	8.06	RyhB	47	66	57	63	3.09	-11.54	-22.69	-1.2134	GUCAUACUGGGAAGUGGUGUG&CACAUUGCUUCCAGUAUUAC	((.(((((((.((((((((((&))))))))))))))))).))	AAUAUUUUCAACUGAGUUAUCAAGAUGUGAUUAGAUUAUUAUUCUUUUACUGUAUCUACCGUUAUCGGAGUGGCUAUGCGAGUUGUCAUACUGGGAAGUGGUGUGGUAGGCGUUGCCAGCGCCUGGUACUUAAAUCAGGCAGGACAUGAG	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0210945	0.9496255	dadA	945752	D-amino acid dehydrogenase
b0728	-74	-59	-66	-60	1.61	RyhB	50	64	51	57	2.98	-10.97	-15.56	-1.15347	AAUGCCCGGAAGCGAU&AUUGCUUCCAGUAUU	(((((..(((((((((&))))))))).)))))	CAAUGCCCGGAAGCGAUACGAAAUAUUCGGUCUACGGUUUAAAAGAUAACGAUUACUGAAGGAUGGACAGAACACAUGAACUUACAUGAAUAUCAGGCAAAACAACUUUUUGCCCGCUAUGGCUUACCAGCACCGGUGGGUUAUGCCUGU	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0284439	0.9496255	sucC	945312	succinyl-CoA synthetase beta subunit
b3928	30	40	33	39	2.04	RyhB	51	61	52	58	2.88	-13.08	-18	-1.37533	ACUGGAAGCAA&UUGCUUCCAGU	(((((((((((&)))))))))))	UCGUAUUUAUUAAGGCGUCACCGGUAAUCGGGACGAGGAUUUUUAUCCAUCAACGCCUUGCAAUUCAGGAGAGGUAUGACAAUGUCAUUAGAAGUGUUUGAGAAACUGGAAGCAAAAGUACAGCAGGCGAUUGAUACCAUCACUCUGUUG	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0091526	0.9291058	zapB	948420	FtsZ stabilizer septal ring assembly factor cell division stimulator
b3777	15	47	35	41	9.19	RyhB	51	84	56	62	14.45	-11.41	-35.05	-1.19973	CAGCCCUAAGGUUGGUGAAAUACUGGAAUGCAA&UUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUG	((((((...(((((((((((((((((((.((((&))))))))))))))..))).)))))).)).))))	CAUUAGACUUAGAAAAUAUCGGGUCGUAACGUUGUGUUAUUACUUUUUCAGAGUUUACUAAGGAGAGGUUUACUCGUGGCGAUUAAUUUCAGCCCUAAGGUUGGUGAAAUACUGGAAUGCAACUUUGGUAACUAUCCUGUUAGCCAAAAU	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0225939	0.9496255	yifN	2847676	PemK toxin family pseudogene
b2283	-15	-6	-13	-7	0.75	RyhB	52	61	53	59	2.88	-13.04	-16.67	-1.37112	ACUGGAAGCA&UGCUUCCAGU	((((((((((&))))))))))	ACCUGCUGAAAGAGCGCUGGUAACCGAAUUUCGAUUAACGCUCAGUCUCUGACUGAGAAAACUGGAAGCAUGCUAAUGGCUACAAUUCAUGUAGACGGCAAAGAAUACGAGGUCAACGGAGCGGACAACCUGCUGGAAGCUUGUCUGUCU	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0093579	0.9291058	nuoG	946762	NADH:ubiquinone oxidoreductase chain G

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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