Input and runtime details for job 4627738 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_003210
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 4627738 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:20:43 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:22:37 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:24:00 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:24:55 CET 2020
	Job Completed		@ Mon Jan 27 14:25:04 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=4627738&toolName=IntaRNA ( 30 days expiry )

Description of the job

LhrA2 - Listeria monocytogenes

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_003210 Listeria monocytogenes EGD-e

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
lmo0292	3	49	39	45	1.83	LhrA2	155	203	159	165	7.05	-14.32	-23.2	-1.35026	GGACGAGAAAGAAAAGAAUUUAAAUGAAAACAGCGAGAAUGAAAGCA&UGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCC	(((.(((..(((...(((((...(((........(((((((((((((&))))))))))).))........)))..)))))...)))....))).)))	UUUUCAUCGUUUUUUCAUAUUUGGGGUUUAUUAUGGAAGUAUAUAAAUGCGAAAUUUGCAGAGAGGAAGGAAGACAUGGACGAGAAAGAAAAGAAUUUAAAUGAAAACAGCGAGAAUGAAAGCACGCCGAAAAGAGAGGUCGAGGAUACU	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.0218921	0.9474659		987455	heat-shock protein htrA serine protease
lmo2768	30	51	41	47	4.37	LhrA2	161	179	165	171	2.1	-16.16	-22.63	-1.52376	AUGGCGUAAUAUGAGAUGGAUG&CAUUCAUCUCAUUGGCCGU	(((((.....((((((((((((&))))))))))))..)))))	UUUCACGGUGCAUUUAGCAAAUGAUAAAAUAGAUUAUUACGAGAUUUAAAACAGACGGGAGGGACAAGGCUAAUAAUGUUUAAUCGAGGGUUAUGGUACCGGGAAUGGCGUAAUAUGAGAUGGAUGCUUUUGGGUGUUGUGAUACUAUUU	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.0081436	0.863511		986273	hypothetical membrane protein
lmo2797	-16	53	47	53	11.21	LhrA2	153	209	153	159	8.55	-13.46	-33.22	-1.26917	AUAAGGGGGUACGGGCAUGUUAUUGGACCGAGUAACUUUAGAGGAUAUUGAGGUAAAUUUAAAAGCAGA&UUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAU	((((.(((((...((((......(((((.....((((.....((....((((((.(((...((((((((&)))))))).))).))))))....))....))))...)))))))))..))))).))))	UUGGAGCGAGAGUCGCUGGCGGAGGUAGAUUUAACAGAAAUAAUGGCUGAAUAUAAUAAAUAAGGGGGUACGGGCAUGUUAUUGGACCGAGUAACUUUAGAGGAUAUUGAGGUAAAUUUAAAAGCAGAAGACUGGCGGGAAGCUAUCCAA	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.0339368	0.9493467		986733	PTS mannitol transporter subunit IIA
lmo0196	-2	56	47	53	14.05	LhrA2	156	208	159	165	7.39	-15.48	-36.92	-1.45964	UAAUGGAGAUUACAGAUGUGAGAUUACGACGUGUUGAGACAGAUGGGAGAAUGAAAGC&GUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUA	((((((.(((...((((((((.(((((.(((.(((((......(((((((((((((((&)))))))))).)))))))))))))))))))))))))).......)))))))))	UUUCUCGAGUGUGUACUUAAGUUGCAUGUGAGCAUUGUUUACUAGCAGCGCAACUAGAUUAAGGUGGUGAAGAUAAUGGAGAUUACAGAUGUGAGAUUACGACGUGUUGAGACAGAUGGGAGAAUGAAAGCUAUUUCUUCAAUAACGAUU	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.0118324	0.8692263		987023	regulatory protein SpoVG
lmo0198	29	59	47	53	7.11	LhrA2	153	183	159	165	3.17	-13.57	-23.85	-1.27954	CUGCUGGCCAAGGCACACGGAUGAAAUCAAA&UUUGUUUUCAUUCAUCUCAUUGGCCGUGUAG	((((.((((((.......((((((((.((((&)))).))))))))......))))))..))))	UGCAUUCUUGAAAAAAUGUAAAUUUUACGUUAUCAUUCAUAGUGGAUGAAUAGUGAAUAUUGGAGGUUCUAUAUAAUGUCAAAACGAUAUGCUGUAGUGCUUGCUGCUGGCCAAGGCACACGGAUGAAAUCAAAACUUUACAAAGUAUUG	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.0321135	0.9493467	glmU	987026	bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase
lmo2299	37	63	56	62	6.01	LhrA2	155	181	156	162	2.25	-15.54	-23.8	-1.4653	AUGCGGAGAAUGGGACUAUUGAAAGCA&UGUUUUCAUUCAUCUCAUUGGCCGUGU	((((((..(((((((....((((((((&))))))))....)))))))..))))))	ACGUGUGAUGCUUUUCAGUACUAUGUAAAAGACAAUCUAAGGAAGUUAGGGCUCAAAUACUAGGGGGGGGAAACCUUGAUUAACCAAAUAAUCGCAAGCGUGAAAGGAGUGAUGCGGAGAAUGGGACUAUUGAAAGCACUGAAAGAUGUA	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.0114532	0.863511		984584	portal protein
lmo1767	11	63	28	34	14.05	LhrA2	158	213	191	197	8.5	-13.96	-36.51	-1.31632	AUGCAUAUAGUAAAGCAGGCGUGGACGUAGAAGCUGGCUAUCAAGUAGUGGAA&UUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAU	(((((((.........((((((((((((.(((.(((((((...((.(((((((&)))))))...))...)))))....)))))))))))))))))........)))))))	ACCCCUUUAUACGAUUAUGAAGCGGAAUAUUUAGCGAGCUUAGAAGCAGAAAAAUAAUGAGGAGUGACAAAUCGAAUGGCAGAAAAUGCAUAUAGUAAAGCAGGCGUGGACGUAGAAGCUGGCUAUCAAGUAGUGGAACGCAUCAAAAAA	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.0263503	0.9474659	purM	985975	phosphoribosylaminoimidazole synthetase
lmo0383	24	64	58	64	7.65	LhrA2	151	186	151	157	4.14	-18.44	-30.23	-1.73875	GAAUUAUAUUGACGGCGAAUGGGUCGAGAGUAAGACAGAUA&UAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUC	((((((((....((((.((((((....((((((((((((((&)))))))))).))))..)))))).))))))))))))	UUUUAUUGGCUUAAUAUGGGGGUAUAAAUCGAUUGAUGGUUAACUUAUGAGUAAUUUUAAGUGGAGGGAUUAUUUAUGGCAGAUGUACGGAAAUUAAAGAAUUAUAUUGACGGCGAAUGGGUCGAGAGUAAGACAGAUAAAUAUGAAGAU	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.002162	0.863511		987639	methylmalonate-semialdehyde dehydrogenase
lmo1798	21	65	21	27	3.39	LhrA2	129	169	163	169	6.56	-13.42	-23.37	-1.2654	AGAUGAACUUGAAAAUAAAAUUAUUGAUCAAUUGACAGAAGCAGA&UCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCU	(((((((..((((((((((..((.((((..........(((((((&)))))))..........)))).)))))))))))))))))))	AUAGAGCCACAAUACAACGAUUCCCUGUCGCUAAGAAAAUUUAGCGGCAGGGAAAUUAUAAAAGGAGUGGUACAUAUGCCUUGGAGAGGUAUAUUAGAUGAACUUGAAAAUAAAAUUAUUGAUCAAUUGACAGAAGCAGAAGAGCACGCC	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.034623	0.9493467		985932
lmo0482	44	69	61	67	3.37	LhrA2	172	210	174	180	7.54	-15.99	-26.9	-1.50773	CAGAAUGGCUAGAGGCACACGGUCAA&UUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUG	((.(((((...(((((((((((((((&)))))))))).............)))))...))))).))	AAUAGCAUUGCUUGUGAACCAUAAAACAGCUAUAAUCUUUAUAGACGAAAACAAGACCUGAAAAGAGGCUGAACAAUGGAAAAAAGCUCCAUUUAUGGAUUAACAUGGACAAAUUUAACAGAAUGGCUAGAGGCACACGGUCAAAAGAAA	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.008949	0.863511		986229	ribosomal RNA large subunit methyltransferase N

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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