Input and runtime details for job 7814698 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_002516
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 7814698 (server version trunk)

	Job Submitted & Queued		@ Fri Jan 07 21:23:03 CET 2022
	Pre-Processing Started		@ Fri Jan 07 21:23:24 CET 2022
	IntaRNA Started		@ Fri Jan 07 21:25:48 CET 2022
	IntaRNA Finished & Post-Processing		@ Fri Jan 07 21:26:49 CET 2022
	Post-Processing Finished		@ Fri Jan 07 21:27:12 CET 2022
	Job Completed		@ Fri Jan 07 21:27:13 CET 2022

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=7814698&toolName=IntaRNA ( 30 days expiry )

Description of the job

PrrF1 - Pseudomonas aeruginosa

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_002516 Pseudomonas aeruginosa PAO1

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
PA5070	13	29	23	29	3.11	PrrF1	104	121	104	110	2.23	-11.88	-17.22	-1.18469	AAACCCGAGCAGCCUGA&UCAGGCUAAUCACGGUUU	(((((.((..(((((((&)))))))..))..)))))	CGCGGACGCCGCGGCACCGCCGGCAGUCGCUGCCUCCACUCCCCCUUCGCCACCGUCCGAGACGCCGCGUAAUCCAUGAGCGCCGAUAAACCCGAGCAGCCUGAGCACGACCAGGAAAUGCCCCUGGUCUCGCACCUGACCGAACUGCGU	UUGACAUGGAAAUGAGAAUCAUUAUUAUGUCACUCAACUGGUCGCGAGAUCAGCCGGUAAGCUGAGAGACCCACGCAGUCGGACUCUUCAGAUUAUCUCCUCAUCAGGCUAAUCACGGUUUUUGACCCGGCACUUUGCCGGGUCUUUUUUU	0.0167935	0.9556607	tatC	879671	transporter TatC
PA2741	-74	-54	-66	-60	4.49	PrrF1	74	91	78	84	5.88	-11.04	-21.41	-1.10093	CUGAACAAGUCUGACGUUGCG&CGCAGUCGGACUCUUCAG	(((((..((((((((..((((&)))))))))))).)))))	GCUGAACAAGUCUGACGUUGCGCGCGUAGAACGCUCCCUGCGUCUGCGCUGAUUAUUAAGGUAGAGGAUUAAUUCAUGGCUCGUGUUAAGCGUGGCGUUAUCGCCCGUCGUCGUCACAAGAAAAUUCUGAAGCUCGCCAAGGGCUACUAC	UUGACAUGGAAAUGAGAAUCAUUAUUAUGUCACUCAACUGGUCGCGAGAUCAGCCGGUAAGCUGAGAGACCCACGCAGUCGGACUCUUCAGAUUAUCUCCUCAUCAGGCUAAUCACGGUUUUUGACCCGGCACUUUGCCGGGUCUUUUUUU	0.027159	0.9556607	rplT	882982	50S ribosomal protein L20
PA0059	-61	-42	-51	-45	10.39	PrrF1	73	91	76	82	5.88	-11.24	-27.51	-1.12087	CUGAACUUGUCCGGCUGCGU&ACGCAGUCGGACUCUUCAG	(((((...((((((((((((&))))))))))))..)))))	GCCGCGCAAGCGGACUGAACUUGUCCGGCUGCGUCGUCCUCCCAUGUAGACAUGCCCGCUCGCCGACGCGAGCGGAUGUCCAUCCACAGCUCCGGAGGAGUCGACAUGAAGAAGACGGCAUCGGCGGUCUGGCAAGGCGGCCUGAAAGAC	UUGACAUGGAAAUGAGAAUCAUUAUUAUGUCACUCAACUGGUCGCGAGAUCAGCCGGUAAGCUGAGAGACCCACGCAGUCGGACUCUUCAGAUUAUCUCCUCAUCAGGCUAAUCACGGUUUUUGACCCGGCACUUUGCCGGGUCUUUUUUU	0.0242675	0.9556607	osmC	878070	osmotically inducible protein OsmC
PA2659	-9	17	10	16	3.85	PrrF1	75	99	76	82	6.14	-15.78	-25.77	-1.57361	GAGAUUCCCAUGAAGAAACUGACUGC&GCAGUCGGACUCUUCAGAUUAUCUC	(((((.....((((((..((((((((&))))))))..))))))....)))))	AGCUUCAAUUAAGAGCGCCCGGUUAAUCUGCAAACCGUCGAAAGCAACACGAUUGAUUCCCACGGAGAGAUUCCCAUGAAGAAACUGACUGCCCUGUUCGCCGCCACCGCCAUCGCCCUGACUGCCGGCCUGGCCCAGGCCCGCGACCUC	UUGACAUGGAAAUGAGAAUCAUUAUUAUGUCACUCAACUGGUCGCGAGAUCAGCCGGUAAGCUGAGAGACCCACGCAGUCGGACUCUUCAGAUUAUCUCCUCAUCAGGCUAAUCACGGUUUUUGACCCGGCACUUUGCCGGGUCUUUUUUU	0.0013402	0.5771389		882368	'Product name confidence: class 4 (Homologs of previously reported genes of unknown function or no similarity to any previously reported sequences)'
PA0916	-13	-1	-8	-2	2.41	PrrF1	107	119	108	114	2.23	-13.58	-18.22	-1.35422	ACCGAGGUUAGCC&GGCUAAUCACGGU	((((.((((((((&)))))))).))))	CGCGGCGCUGCCGCUAGAAUUGCGCACUUUUUGAUCAGAGGCGCCAGCAAGCGCGCCCGCCUACCGAGGUUAGCCAUGUCCACCCCCACUCCCAAAGUCGGCUUCGUCAGUCUUGGAUGCCCCAAGGCGCUGGUCGACUCCGAGCGCAUC	UUGACAUGGAAAUGAGAAUCAUUAUUAUGUCACUCAACUGGUCGCGAGAUCAGCCGGUAAGCUGAGAGACCCACGCAGUCGGACUCUUCAGAUUAUCUCCUCAUCAGGCUAAUCACGGUUUUUGACCCGGCACUUUGCCGGGUCUUUUUUU	0.005944	0.9377188		880629	ribosomal protein S12 methylthiotransferase RimO
PA3255	-74	-60	-67	-61	2.74	PrrF1	101	115	102	108	2.12	-15.04	-19.9	-1.49981	UGAUGAGCCUGGUGA&UCAUCAGGCUAAUCA	((((.((((((((((&)))))))))).))))	UUGAUGAGCCUGGUGAUCUUCGACCUGGACGAGACCAUGAUCCACGGCGACUGCUCCAGCCUGUGGAGCCAGCGCAUGGCGAGUCUCGGCUGGGUCGACGAAUCCUUCGUCCGCCGCGACGCCGAACUGAUGGAGCAGUACGCCGAAGGC	UUGACAUGGAAAUGAGAAUCAUUAUUAUGUCACUCAACUGGUCGCGAGAUCAGCCGGUAAGCUGAGAGACCCACGCAGUCGGACUCUUCAGAUUAUCUCCUCAUCAGGCUAAUCACGGUUUUUGACCCGGCACUUUGCCGGGUCUUUUUUU	0.0022566	0.6680946		882418	'Product name confidence: class 4 (Homologs of previously reported genes of unknown function or no similarity to any previously reported sequences)'
PA3263	-66	-51	-58	-52	9.16	PrrF1	104	119	105	111	2.23	-11.58	-22.97	-1.15478	ACCGGGGUUGGCCUGA&UCAGGCUAAUCACGGU	((((.(((((((((((&))))))))))).))))	UAGCGACGGACCGGGGUUGGCCUGAGCCGCCAGCGGCGGAAUAAUGGGUCCUUUGUGCCCGGAGAGAACGACCCCAUGUGGUUCCGCAAUCUGCUCGUCUACCGCCUCACCCAGGAUCUGCAACUGGAUGCCGAUUCCCUCGAGAAAGCC	UUGACAUGGAAAUGAGAAUCAUUAUUAUGUCACUCAACUGGUCGCGAGAUCAGCCGGUAAGCUGAGAGACCCACGCAGUCGGACUCUUCAGAUUAUCUCCUCAUCAGGCUAAUCACGGUUUUUGACCCGGCACUUUGCCGGGUCUUUUUUU	0.0199868	0.9556607		882426	recombination associated protein RdgC
PA5489	-23	26	12	18	9.41	PrrF1	6	53	14	20	18.7	-11.08	-39.19	-1.10492	CUGACCUCGCCUAGGAGUGAACGAUGCGUAACCUGAUUCUCACCGCCAU&AUGGAAAUGAGAAUCAUUAUUAUGUCACUCAACUGGUCGCGAGAUCAG	((((.(((((((((((((((......(((((..(((((((((...((((&))))...)))))))))...)))))))))))..))))..))))).))))	GACCGCUCCCCCCGGUCGAACUGACCGGGCCGAACCCGGCCGGAGAGCGGCACUGACCUCGCCUAGGAGUGAACGAUGCGUAACCUGAUUCUCACCGCCAUGCUGGCCAUGGCCAGCCUGUUCGGCAUGGCCGCCCAGGCCGACGACUAU	UUGACAUGGAAAUGAGAAUCAUUAUUAUGUCACUCAACUGGUCGCGAGAUCAGCCGGUAAGCUGAGAGACCCACGCAGUCGGACUCUUCAGAUUAUCUCCUCAUCAGGCUAAUCACGGUUUUUGACCCGGCACUUUGCCGGGUCUUUUUUU	0.0265569	0.9556607	dsbA	877731	thiol:disulfide interchange protein DsbA
PA1196	36	55	47	53	7.22	PrrF1	103	121	105	111	2.23	-11.73	-21.18	-1.16973	AAGCGGUGAACUGGCCUGGU&AUCAGGCUAAUCACGGUUU	((((.((((..(((((((((&))))))))).)))).))))	GGAUGCUCGGUCGCGCUGCGUCGAAGAUGGGACAACUGACGAGAUUUCGACAUCCGGGAAACCGAGGGGGAGAGCAUGGCGCUUGUCGAGGAACCGACGAUUUCCACGCGAAGCGGUGAACUGGCCUGGUUGGUGGACUGCCUGGCGCCG	UUGACAUGGAAAUGAGAAUCAUUAUUAUGUCACUCAACUGGUCGCGAGAUCAGCCGGUAAGCUGAGAGACCCACGCAGUCGGACUCUUCAGAUUAUCUCCUCAUCAGGCUAAUCACGGUUUUUGACCCGGCACUUUGCCGGGUCUUUUUUU	0.0183269	0.9556607		878419	transcriptional regulator
PA0898	-56	-29	-41	-35	10.07	PrrF1	99	122	104	110	2.24	-12.8	-25.11	-1.27644	AGAAGCGCGGUUGAAAGCCUGAUGGAGG&CCUCAUCAGGCUAAUCACGGUUUU	((((.((.((((...((((((((.((((&)))))))))))))))).)).))))	GCGGUGCCGCUGUCGGCGCAGAAGCGCGGUUGAAAGCCUGAUGGAGGCGUCCCGGCGAGGGCGCCCGAGGAGUCGAUGAUGAGUACCCAUUACAUUGCCGGCCAGUGGCUGGCGGGCCAGGGCGAGACCCUGGAGUCCCUCGACCCGGUU	UUGACAUGGAAAUGAGAAUCAUUAUUAUGUCACUCAACUGGUCGCGAGAUCAGCCGGUAAGCUGAGAGACCCACGCAGUCGGACUCUUCAGAUUAUCUCCUCAUCAGGCUAAUCACGGUUUUUGACCCGGCACUUUGCCGGGUCUUUUUUU	0.0096811	0.9556607	aruD	883040	N-succinylglutamate 5-semialdehyde dehydrogenase

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.2.0 linking Vienna RNA package 2.4.14

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching