Input and runtime details for job 3751556 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_000913
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 3751556 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:09:18 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:12:06 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:12:59 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:14:52 CET 2020
	Job Completed		@ Mon Jan 27 14:15:02 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3751556&toolName=IntaRNA ( 30 days expiry )

Description of the job

CyaR - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
b3168	49	67	61	67	11.55	CyaR	29	45	29	35	4.3	-11.86	-27.71	-1.25616	GUGGAACGCCUGGUACAGC&GCUGUACCAGGAACCAC	((((....(((((((((((&)))))))))))..))))	GCACUGAUUAUGGCUGCCCGUAAUAUUUGCUGGUUCGGUGACGAAGCGUAAUAAACUGUAGCAGGAAGGAACAGCAUGACAGAUGUAACGAUUAAAACGCUGGCCGCAGAGCGACAGACCUCCGUGGAACGCCUGGUACAGCAAUUUGCU	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0171203	0.9277422	infB	947684	translation initiation factor IF-2
b3197	10	40	34	40	9.83	CyaR	1	35	1	7	1.49	-13.72	-25.04	-1.45317	GUAGAGUUACAACCGGGUUUUGACUUUCAGC&GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUAC	(((.(((((((...(((((.((..(((((((&)))))))..)).)))))......)).))))).)))	CUGGCGGAUUUAUCGUAUGGCUGGCGAUGUUGUACUGGUUAUCGCCAAUACUCGUUGAAUAACUGGAAACGCAUUAUGUCGCACGUAGAGUUACAACCGGGUUUUGACUUUCAGCAAGCAGGUAAAGAAGUCCUGGCGAUUGAACGUGAA	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0053801	0.8345133	kdsD	947734	D-arabinose 5-phosphate isomerase
b3215	60	73	67	73	5.91	CyaR	36	51	36	42	3.22	-10.88	-20.01	-1.15237	AGGGAUGGUUCCUG&CAGGAACCACCUCCUU	((((((((((((((&)))))))))..)))))	UCGCAAACAGUGCAUAACAUAUAUUAACAAUAUGUUUCUGCGUUAUUUAUCAUUAUUACAGGAGUUUUAAUUUAAAUGCUCAGACACAUUACAUUCACUGUAUUUAUAACAACAUCAAUGAAUACCUUAGCCACAGGGAUGGUUCCUGAA	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0300585	0.9277422	yhcA	947741	putative periplasmic chaperone protein
b3284	-60	-47	-59	-53	2.73	CyaR	16	31	24	30	1.42	-10.6	-14.75	-1.12271	AGUUAGCAGGAUGG&CCAUAAAAUGCUAGCU	((((((((..((((&))))....))))))))	AACUACUCGAACUGGAGUUAGCAGGAUGGAUCGCAGCUGUACCCGGCGGCUAUGUCCGAUUGAGGAGGGCAUGCCAUGUUCGACGUACUAAUGUAUUUGUUUGAAACCUAUAUUCACACAGAAGCUGAGUUGCGUGUGGAUCAAGACAAA	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0351035	0.9277422	smg	947781	DUF494 family putative periplasmic protein
b3412	-55	-38	-45	-39	7.86	CyaR	23	40	24	30	4.55	-11.17	-23.58	-1.18308	UCCCGGUACUGUUAGCAU&AUGCUAGCUGUACCAGGA	(((.(((((.((((((((&)))))))).))))).)))	AUUCGGCAUAGCCAGCAUAAUCCCGGUACUGUUAGCAUAUGUUCAUCCUUGUAAGUCAAAAGAGAACAAUAGCGGAUGAAUAACAUCUGGUGGCAGACCAAAGGUCAGGGGAAUGUUCAUCUUGUGCUGCUGCACGGAUGGGGACUGAAU	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0255288	0.9277422	bioH	947916	pimeloyl-ACP methyl ester carboxylesterase
b3423	37	59	51	57	6.83	CyaR	9	38	11	17	4.53	-12.11	-23.47	-1.28264	CUGGUUAAACAGCAGGGUUAUGU&ACAUAACCCAUAAAAUGCUAGCUGUACCAG	(((((...(((((.(((((((((&)))))))))...........))))))))))	UAGCCGUGGGUUUAGCGAUGGCUUUUGUUGAUUCGCUCAAUGCGCGAAAACGAAAAUAAUUCCAGGGAUUUAUAAAUGAAACAAACACAACGUCACAACGGUAUUAUCGAACUGGUUAAACAGCAGGGUUAUGUCAGUACCGAAGAGCUG	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0147559	0.9277422	glpR	947926	pseudogene DNA-binding transcriptional repressorregulator Energy metabolism carbon: Anaerobic respiration repressor of the glp operon
b3426	27	53	46	52	11.1	CyaR	23	50	24	30	4.61	-10.8	-26.51	-1.14389	AGGGGGCGGCAUCAAUGGUGCUGGUAU&AUGCUAGCUGUACCAGGAACCACCUCCU	((((((.((..((..((((((((((((&))))))))...)))).)).)).))))))	CCUAAAGCGCGAUUCUUUGCUAAUAUGUUCGAUAACGAACAUUUAUGAGCUUUAACGAAAGUGAAUGAGGGCAGCAUGGAAACCAAAGAUCUGAUUGUGAUAGGGGGCGGCAUCAAUGGUGCUGGUAUCGCGGCAGACGCCGCUGGACGC	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.031429	0.9277422	glpD	947934	sn-glycerol-3-phosphate dehydrogenase aerobic FAD/NAD(P)-binding
b3456	45	74	68	74	9.45	CyaR	15	42	15	21	4.57	-14.38	-28.4	-1.52307	GUUCUUUGUGCUGGCGGGCGUCUUUAUGGG&CCCAUAAAAUGCUAGCUGUACCAGGAAC	((((((.((((..((..((((.((((((((&))))))))))))..)).)))).))))))	CUCAUUCGCCCUGCUGAUUCUGGUGCUGCUGGUGAUGCCGACCGGUAUUCUGGGUCGCCCGGAGGUAGAGAAAGUAUGAAACCGAUGCAUAUUGCAAUGGCGCUGCUCUCUGCCGCGAUGUUCUUUGUGCUGGCGGGCGUCUUUAUGGGC	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0034603	0.779419	livM	947966	branched-chain amino acid ABC transporter permease
b3482	42	71	42	48	8.56	CyaR	1	35	29	35	1.49	-12.85	-22.9	-1.36102	GUAUGGCGGUAGCAUUGUUCAGGGUUCAGC&GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUAC	(((((((..(((((((.....((((.((((&)))).........))))....))))))))))))))	CAUUGUGACCACAAGUUUUUCUUCGCUUUUUCGUAUGAAGAUACUGUCAUUAAAAUAAUAGAAAAGGAUUUUACGAUGAGCGGAAAACCGGCGGCGCGUCAGGGUGACAUGACGCAGUAUGGCGGUAGCAUUGUUCAGGGUUCAGCCGGG	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0093869	0.9277422	rhsB	947994	Rhs protein with DUF4329 family putative toxin domain putative neighboring cell growth inhibitor
b3492	28	49	35	41	10.02	CyaR	9	43	29	35	4.6	-10.65	-25.27	-1.128	GGCGCUGGUGCGGCGGGUAUGU&ACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACC	((..((((((((((((((((((&)))).))))...........))))))))))...))	UAGUAAUAUUGAUGCUGGUGCGACCACUGAGGAAUCUUUACAAUUCACGCCCGUUUUUUCUAAGAGGAGCGCAACGUGGAAAGGUUUGAUGCCAUUAUUAUAGGCGCUGGUGCGGCGGGUAUGUUCUGUUCUGCGCUGGCAGGUCAGGCA	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0341505	0.9277422	yhiN	947996	putative oxidoreductase

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching