Input and runtime details for job 3751556 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_000913
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 3751556 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:09:18 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:12:06 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:12:59 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:14:52 CET 2020
	Job Completed		@ Mon Jan 27 14:15:02 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3751556&toolName=IntaRNA ( 30 days expiry )

Description of the job

CyaR - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
b3456	45	74	68	74	9.45	CyaR	15	42	15	21	4.57	-14.38	-28.4	-1.52307	GUUCUUUGUGCUGGCGGGCGUCUUUAUGGG&CCCAUAAAAUGCUAGCUGUACCAGGAAC	((((((.((((..((..((((.((((((((&))))))))))))..)).)))).))))))	CUCAUUCGCCCUGCUGAUUCUGGUGCUGCUGGUGAUGCCGACCGGUAUUCUGGGUCGCCCGGAGGUAGAGAAAGUAUGAAACCGAUGCAUAUUGCAAUGGCGCUGCUCUCUGCCGCGAUGUUCUUUGUGCUGGCGGGCGUCUUUAUGGGC	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0034603	0.779419	livM	947966	branched-chain amino acid ABC transporter permease
b3426	27	53	46	52	11.1	CyaR	23	50	24	30	4.61	-10.8	-26.51	-1.14389	AGGGGGCGGCAUCAAUGGUGCUGGUAU&AUGCUAGCUGUACCAGGAACCACCUCCU	((((((.((..((..((((((((((((&))))))))...)))).)).)).))))))	CCUAAAGCGCGAUUCUUUGCUAAUAUGUUCGAUAACGAACAUUUAUGAGCUUUAACGAAAGUGAAUGAGGGCAGCAUGGAAACCAAAGAUCUGAUUGUGAUAGGGGGCGGCAUCAAUGGUGCUGGUAUCGCGGCAGACGCCGCUGGACGC	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.031429	0.9277422	glpD	947934	sn-glycerol-3-phosphate dehydrogenase aerobic FAD/NAD(P)-binding
b2556	40	58	48	54	6.05	CyaR	20	38	24	30	4.48	-15.09	-25.62	-1.59827	CUGGUAAUGCUGGCAUUUU&AAAAUGCUAGCUGUACCAG	((((((..(((((((((((&)))))))))))..))))))	UAAUUCCCGGCUUUGUUGUAUCUGAACUUCCCCUCGGUUAGCAUCAGGCUAUUCGCGUCUGACGAGAGUAACACCUUGAAACGCUGGCCCGUUUUUCCCCGCUCAUUACGACAACUGGUAAUGCUGGCAUUUUUGCUGAUUCUGCUGCCC	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0021103	0.7364947	glrK	947013	sensor protein kinase regulating glmY sRNA in two-component system with response regulator GlrR
b2308	-11	19	7	13	12.62	CyaR	2	36	14	20	1.49	-12.74	-26.85	-1.34937	GGACAGGCAGCAUGUUGUAUGGGUUUUCAG&CUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACC	((((((..(((((....(((((((((((((&))))))......)))))))..)))))..)))).))	AGAAAGCCCGCUCCCUUCGGGUAACCGGAGGGAGACAAGAGGCGGUAAUUCACCACACACGACAGGACAGGCAGCAUGUUGUAUGGGUUUUCAGGUGUUAUUUUACAGGGUGCGCUCGUCACGCUGGAGCUGGCUAUCAGCUCUGUAGUG	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0100518	0.9277422	hisQ	947235	histidine ABC transporter permease
b0444	28	64	54	60	13.08	CyaR	23	49	30	36	4.55	-11.11	-28.74	-1.17673	GGAGGUCAGGAUUCCACCACCUGUCUGGUGCAGGCAU&AUGCUAGCUGUACCAGGAACCACCUCC	((((((..((.((((..........((((((((((((&))))...))))))))))))))))))))	CAUCGCAAUGAAUAGCUGGUCCGGGUUAAUCGCAGGCGUUACCAUAACGCUUUUAUUGUUCAAGCAGGAUUAUCUAUGAAACGUGCUGUCGUUGUGUUCAGUGGAGGUCAGGAUUCCACCACCUGUCUGGUGCAGGCAUUACAACAAUAU	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0264121	0.9277422	queC	947034	7-cyano-7-deazaguanine (preQ0) synthase queuosine biosynthesis
b1041	54	73	67	73	5.82	CyaR	10	34	10	16	1.48	-11.4	-18.7	-1.20744	UGCAGCCGCAGCAGGUUAUG&CAUAACCCAUAAAAUGCUAGCUGUA	((((((...(((((((((((&))))))).......)))).))))))	AAAUACAGGUUGCGUUAACAACCAAGUUGAAAUGAUUUAAUUUCUUAAAUGUACGACCAGGUCCAGGGUGACAACAUGAAAAACAAAUUGUUAUUUAUGAUGUUAACAAUACUGGGUGCGCCUGGGAUUGCAGCCGCAGCAGGUUAUGAU	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.022384	0.9277422	csgB	947391	curlin nucleator protein minor subunit in curli complex
b4547	23	35	29	35	2.33	CyaR	34	46	34	40	3.2	-13.97	-19.5	-1.47965	GGUGGAUUCUGGU&ACCAGGAACCACC	(((((.(((((((&))))))).)))))	CUACUUGCCCGUAUGUAUCAACGUAUCAUGGAACAGCUCGUCGCCUGAUGAGUGGUUCUGCAAGAGGAAAUAAGCAUGGACUGGCUGGCUAAAUAUUGGUGGAUUCUGGUGAUUGUCUUUUUGGUAGGCGUCCUGCUGAACGUGAUUAAA	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.004561	0.8345133	ypfN	1450286	putative membrane protein UPF0370 family
b2568	40	60	54	60	7.67	CyaR	16	38	16	22	4.49	-12.94	-25.1	-1.37055	CUGGUGACGGGCAUUUUAUGG&CCAUAAAAUGCUAGCUGUACCAG	(((((.(((((((((((((((&)))))))))))...)))))))))	GUCGAGCUGCCGCAGGAAGCGUUCCUCGCCAUUCUGCACGUCGGCAAAGACAACAAAUAACCCUUAGGAGUUGGCAUGGCGAAUAUGUUUGCCCUGAUUCUGGUGAUUGCCACACUGGUGACGGGCAUUUUAUGGUGCGUGGAUAAAUUC	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0088729	0.9277422	lepB	947040	leader peptidase (signal peptidase I)
b0684	-15	27	21	27	11.55	CyaR	1	48	1	7	4.6	-10.55	-26.7	-1.11741	GAGGUUAUUUCACUCAUGGCUAUCACUGGCAUCUUUUUCGGC&GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUC	(((((..((((.....(((.((.(((((((((.(((((((((&))))))))).............))))))).)))))))))))..)))))	UUCUGCUAUGAUUGCCUUUAUCCGUGGGCAAUUUUCCACCCCCAUUUCAAUAAGUUUCAAGAGGUUAUUUCACUCAUGGCUAUCACUGGCAUCUUUUUCGGCAGCGACACCGGUAAUACCGAAAAUAUCGCAAAAAUGAUUCAAAAACAG	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0360802	0.9277422	fldA	945293	flavodoxin 1
b2036	-19	3	-4	3	4.4	CyaR	17	38	17	23	4.49	-10.41	-19.3	-1.10258	CUGGUUUGCUGAGGAUUUUAUG&CAUAAAAUGCUAGCUGUACCAG	(((((..(((.((.((((((((&)))))))).)))))...)))))	CAACAGAGUGCUUAGUUACGUCACUCAUGCUGAUGUUCGUAAGAAAUAAUAAAUUACUGGUUUGCUGAGGAUUUUAUGUACGAUUAUAUCAUUGUUGGUUCUGGUUUGUUUGGUGCCGUUUGUGCGAAUGAGUUAAAAAAGCUAAACAAA	GCUGAAAAACAUAACCCAUAAAAUGCUAGCUGUACCAGGAACCACCUCCUUAGCCUGUGUAAUCUCCCUUACACGGGCUUAUUU	0.0389456	0.9277422	glf	945235	UDP-galactopyranose mutase FAD/NAD(P)-binding

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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