Input and runtime details for job 1972078 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_000913
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 1972078 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:13:56 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:16:07 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:17:05 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:18:21 CET 2020
	Job Completed		@ Mon Jan 27 14:18:31 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1972078&toolName=IntaRNA ( 30 days expiry )

Description of the job

MicC - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
b1776	-72	8	-60	-54	13.29	MicC	8	70	57	63	12.15	-10.51	-35.95	-1.08328	AUGUUGACCUCAAAAAUCAUGUGGGUAAGUAUGAUGGCCAUCUAAGCAAAACUAAACACAGAGGAUGACGCAAUGAAAGC&GCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAU	((((((......(((((((.(((.(((((((..((((((.......((.........(((((((((((..(((((((.((&)).))))))).........)))))))))))))))))))))).)))))))))))))).))))))	UUAAUGUUGACCUCAAAAAUCAUGUGGGUAAGUAUGAUGGCCAUCUAAGCAAAACUAAACACAGAGGAUGACGCAAUGAAAGCACUGGCUCGGUUUGGCAAGGCCUUUGGCGGCUACAAGAUGAUUGAUGUCCCACAACCCAUGUGUGGC	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.018624	0.9679271	ydjL	946299	putative Zn-dependent NAD(P)-binding oxidoreductase
b1817	-20	-10	-16	-10	0.62	MicC	9	19	9	15	0.94	-9.88	-11.44	-1.01835	GAUAAUAAAGG&CCUUUAUUGUC	(((((((((((&)))))))))))	CGAUUCGAUUGUGGACGACGAUUCAAAAAUACAUCUGGCACGUUGAGGUGUUAACGAUAAUAAAGGAGGUAGCAAGUGACCAUUGCUAUUGUUAUAGGCACACAUGGUUGGGCUGCAGAGCAGUUGCUUAAAACGGCAGAAAUGCUGUUA	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.0270603	0.9679271	manX	946334	fused mannose-specific PTS enzymes: IIA component/IIB component
b3005	-12	11	3	9	6.42	MicC	46	65	48	54	4.07	-9.93	-20.42	-1.0235	GGAUAAUAUCCGAUGGCAAUGCA&UGCAUUGCCACUGAUUUUCC	(((....(((...((((((((((&))))))))))..)))..)))	GACCUGGAUCUGGAAGCCAGCGCCGCUGCGCAUCCGGUUCGUGUCGCACAAAAAUUACGCGCAGGAUAAUAUCCGAUGGCAAUGCAUCUUAACGAAAACCUCGACGAUAACGGCGAAAUGCAUGAUAUCAACGUGACGCCGUUUAUCGAC	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.0262783	0.9679271	exbD	946345	membrane spanning protein in TonB-ExbB-ExbD complex
b1835	-15	11	2	8	9.53	MicC	35	58	38	44	10.75	-15.19	-35.47	-1.56566	CAGUGGUACAUGCUCGUGGCCCAACA&UGUUGGGCCAUUGCAUUGCCACUG	((((((((.((((...((((((((((&))))))))))..))))))))))))	CACUGCUCCGGCGUGCCUGCGCCGGAGCGUUUAUGCUAAACUGCGCGCCUGUUUUUUUGCCAGUGGUACAUGCUCGUGGCCCAACACACCGUUUAUUUCCCGGACGCCUUUCUGACACAAAUGCGCGAAGCGAUGCCUUCGACGCUCUCA	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.0008759	0.7974595	rsmF	946348	16S rRNA m(5)C1407 methyltransferase SAM-dependent
b1833	43	54	47	53	1.99	MicC	7	18	8	14	3.26	-10.92	-16.17	-1.12554	ACAGUGAGGGCA&UGCCUUUAUUGU	((((((((((((&))))))))))))	UAAAUGCGUUAAUGCUCAAGAUCAUUCCCAUCAUGGGUUAAGAUUAAUGUUAAUUCUUAUUACAUUUGGCACGUCAUGGCUCUUAACACACCACAAAUUACGCCGACAAAAAAGAUAACAGUGAGGGCAAUCGGCGAGGAACUGCCGCGU	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.0145348	0.9679271	yebS	946353	inner membrane PqiA domain protein
b2103	43	53	46	52	2.54	MicC	47	57	48	54	3.03	-10.8	-16.37	-1.11317	AGUGGUGGUGC&GCAUUGCCACU	(((((((((((&)))))))))))	CAGAAGCGAGGGGCCAGGCAGUUUUGUUCCACAUUUCCUUGAUGCGCUCUGGCAAUUGACGCAGGAGGUGCAGGCAUGAAACGAAUUAACGCUCUGACGAUUGCCGGUACUGAUCCGAGUGGUGGUGCGGGGAUUCAGGCCGAUCUUAAA	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.0156347	0.9679271	thiD	946459	hydroxy-methylpyrimidine kinase and hydroxy-phosphomethylpyrimidine kinase
b2091	4	16	7	13	2.38	MicC	50	62	53	59	2.89	-10.87	-16.14	-1.12039	AAAUCAGUGGUGA&UUGCCACUGAUUU	(((((((((((((&)))))))))))))	ACAAGAGAAAGUCGUUCUCGCAGAAUAAUUUUUACCUGAGGGGGAGGUUAUCCCCCUUAACAAUCAGGAGUUUUUAUGAAAUCAGUGGUGAAUGAUACUGAUGGUAUCGUGCGCGUUGCAGAAAGCGUCAUUCCUGAAAUUAAACAUCAG	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.0149839	0.9679271	gatD	946598	galactitol-1-phosphate dehydrogenase Zn-dependent and NAD(P)-binding
b2118	34	64	58	64	5.65	MicC	7	42	7	13	11.66	-10.57	-27.88	-1.08947	GCCCAACUGGAACUGAAAUAUAAAAAAGGCA&UGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGC	(((((((.((((.((((((((((.(((((((&))))))).)))).....)))))).)))).)))))))	UUCUCCGUAAUCGAUGCCAUCACCGCCAGCGAGCUAAUUAACGAUAUUGAAGCCCUGUUUGAAUAAGGAAAGAGCAUGGACAAGGAAUUACCGUGGCUGGCGGAUAACGCCCAACUGGAACUGAAAUAUAAAAAAGGCAAAACGCCGCUC	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.0179646	0.9679271	yehI	946649	DUF4132 domain-containing protein
b2203	42	55	43	49	7.82	MicC	39	54	47	53	4.76	-12.96	-25.54	-1.33581	GGCGAUGCUGGCCC&GGGCCAUUGCAUUGCC	((((((((((((((&))))))..))))))))	GUGCGGUCGCUGCGUGGAUGUCUGUUCUGAGGAUGUAUUUACAAUAACUACACGAUGGAGUUCGGGAGCGAAAUCAUGAAAAGCCAUGACCUGAAGAAAGCGCUGUGUCAAUGGACGGCGAUGCUGGCCCUGGUGGUAAGCGGCGCGGUU	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.0040051	0.8150379	napB	946698	nitrate reductase small cytochrome C550 subunit periplasmic
b2215	-67	-15	-49	-43	14.91	MicC	1	70	42	48	12.16	-21.53	-48.6	-2.21913	AUGAGGGUUAAUCAGUAUGCAGUGGCAUAAAAAAGCAAAUAAAGGCAUAUAAC&GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAU	(((..((..(((((((((((((((((((((((.....((((((((((((((((&))))))))))))))))........))))))..........))))))))))....)))))))..))..)))	ACUGAUUAAUGAGGGUUAAUCAGUAUGCAGUGGCAUAAAAAAGCAAAUAAAGGCAUAUAACAGAGGGUUAAUAACAUGAAAGUUAAAGUACUGUCCCUCCUGGUCCCAGCUCUGCUGGUAGCAGGCGCAGCAAACGCUGCUGAAGUUUAC	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	5.2e-06	0.0190476	ompC	946716	outer membrane porin protein C

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching