Input and runtime details for job 1972078 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_000913
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 1972078 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:13:56 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:16:07 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:17:05 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:18:21 CET 2020
	Job Completed		@ Mon Jan 27 14:18:31 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1972078&toolName=IntaRNA ( 30 days expiry )

Description of the job

MicC - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
b0982	-12	18	1	7	4.13	MicC	22	56	39	45	9.06	-11.1	-24.29	-1.1441	GUGGAUUCAAACAUGGCCCAACUAAAAUUU&AGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCAC	((((........((((((((((((((((((&))))))))......)))))))))).......))))	CCGUGUGAUUUCUCUGCUGGUACCGACGAUUUCCGGGGUGCAUGACCUGACAGAGACUUCACGGUGGAUUCAAACAUGGCCCAACUAAAAUUUAACUCAAUCCUGGUGGUUUGUACCGGCAACAUUUGCCGUUCGCCCAUUGGCGAGCGC	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.0130207	0.9679271	etp	945236	O-antigen capsule forming protein-tyrosine-phosphatase Etk-P dephosphorylase
b3655	46	64	49	55	7.88	MicC	40	59	50	56	6.08	-11.06	-25.02	-1.13997	UUAGUGGCGAUCGCUGGCC&GGCCAUUGCAUUGCCACUGA	(((((((((((.((.((((&))))...)))))))))))))	UAACCAUUCCCGCCUUGAGGAUUGCGCGUAAAUCGUGCAUAACUCCCUUAUGUGCAUUUCACGGGAUGGAAGACCAUGAAAUUUAUUGGGAAGCUGCUUCUCUACAUUCUCAUCGCUCUGUUAGUGGCGAUCGCUGGCCUCUAUUUUCUU	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.0133437	0.9679271	yicH	948171	putative inner membrane-anchored periplasmic AsmA family protein
b0556	-72	-57	-63	-57	3.89	MicC	42	55	42	48	3.5	-11.05	-18.44	-1.13894	UGGCGGUAAGCAAUGG&CCAUUGCAUUGCCA	(((((((..(((((((&))))))))))))))	CGCUGGCGGUAAGCAAUGGAAAGGCCUGAUGACUCGUCGUGAGAUUGAGCGUGAAGUCUGUUUGUGGGGGCAACAGUGAGCAGAGUAACCGCGAUUAUAUCCGCUCUGAUUAUCUGCAUCAUCGUCAGCCUGUCAUGGGCGGUCAAUCAU	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.0134256	0.9679271	rzpD	945929	DLP12 prophage putative murein endopeptidase
b4230	-10	7	1	7	4.75	MicC	47	65	47	53	4.06	-11.03	-19.84	-1.13688	GGAGAACAGUGUGAUGC&GCAUUGCCACUGAUUUUCC	(((((((((((((((((&))))))).)))).))))))	AGGUGGCGGAGAUCCCGCUGGCAGAGCUUUCCGUUCCGGCGAUCAUGAACGCCAUUGCGGCGUAAGGAGAACAGUGUGAUGCCUCAAUCUCUUCCGGACACCACUACGCCAAAAAGGCGCUUUCGCUGGCCGACGGGGAUGCCGCAGCUG	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.0135909	0.9679271	ytfT	948743	putative sugar ABC transporter permease
b1771	-14	4	-9	-3	4.42	MicC	2	19	8	14	3.65	-11	-19.07	-1.13379	GAUAAUGAAGGCAAAUGA&UUAUAUGCCUUUAUUGUC	(((((((((((((.((((&)))).)))))))))))))	GGUGCCACCACCGGCGUAAAAAACAGAAAGCUGGUCGAACAAUUGCUGGAAGAAUACGAAGGAUAAUGAAGGCAAAUGAAAAAGAUACCUUUAGGCACAACGGAUAUUACGCUUUCGCGAAUGGGGUUGGGGACAUGGGCCAUUGGCGGC	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.0138425	0.9679271	ydjG	946283	methylglyoxal reductase NADH-dependent
b4297	57	71	65	71	4.42	MicC	39	53	39	45	4.75	-10.98	-20.15	-1.13173	GCACGCCGAUGGCCC&GGGCCAUUGCAUUGC	(((...(((((((((&)))))))))...)))	CAUCUGAAGAAGCAAAAGAUAAAGUCCACGUGCUGCUUACCGCGCAGGGCAUUUUACCAGUCUGAGGAGAAACUCAUGUCUGUUCGCAAUAUUUUUGCUGACGAGAGCCACGAUAUUUACACCGUCAGAACGCACGCCGAUGGCCCGGAC	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.0140126	0.9679271	yjhG	946829	putative dehydratase
b2445	51	64	58	64	5.87	MicC	50	63	50	56	2.99	-10.98	-19.84	-1.13173	AGAAUCAGUGGCGA&UUGCCACUGAUUUU	((((((((((((((&))))))))))))))	GAAGUUAAUCAAAGCGGAUAUCCACGCCCAGCUGAAGGCCUGUGAUUGCGAUAUUUCUGAAUGACAGGUGGAAUCAUGAAACACGUUUUUAAAUAUCUUGAUUUUGCAGAAGACCGCGAACACGCAGAAUCAGUGGCGACCAAAGAGUUA	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.0140126	0.9679271	yffN	946930	CPZ-55 prophage uncharacterized protein
b0585	33	53	34	40	7.69	MicC	39	56	49	55	4.76	-10.95	-23.4	-1.12864	GUGGCAGUCGAAACAUGGCCC&GGGCCAUUGCAUUGCCAC	((((((((..((...((((((&))))))))..))))))))	AAAUAGUUAUCAAUAAUAUUAUCAAUAUAUUUCUGCAAUCAAUGAAAAAUUGCACAGUAAACAUGGGGUUAUGGUGUGACGGCGUUAAAAGUAGGAAGUGAGAGCUGGUGGCAGUCGAAACAUGGCCCGGAAUGGCAGCGUCUGAAUGAC	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.0142715	0.9679271	fes	945181	enterobactin/ferrienterobactin esterase
b1833	43	54	47	53	1.99	MicC	7	18	8	14	3.26	-10.92	-16.17	-1.12554	ACAGUGAGGGCA&UGCCUUUAUUGU	((((((((((((&))))))))))))	UAAAUGCGUUAAUGCUCAAGAUCAUUCCCAUCAUGGGUUAAGAUUAAUGUUAAUUCUUAUUACAUUUGGCACGUCAUGGCUCUUAACACACCACAAAUUACGCCGACAAAAAAGAUAACAGUGAGGGCAAUCGGCGAGGAACUGCCGCGU	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.0145348	0.9679271	yebS	946353	inner membrane PqiA domain protein
b4664	-70	-61	-70	-64	1.66	MicC	56	65	59	65	0.92	-10.92	-13.5	-1.12554	GGGGAGUCAG&CUGAUUUUCC	((((((((((&))))))))))	CAAUCGGGGAGUCAGCAGGGGGCUGAAACGGGAAAGCCCCUCCCGAAGAAGGGGGCCUUACAGAAGGAAAGGGUUAUGAUGAAGCUCGUCAUCAUACUGAUUGUGUUGUUACUCGUAAGUUUCGCAGCUUAUUAACAGCCAAUCAGAGGG	GUUAUAUGCCUUUAUUGUCACAGAUUUUAUUUUCUGUUGGGCCAUUGCAUUGCCACUGAUUUUCCAACAUAUAAAAAGACAAGCCCGAACAGUCGUCCGGGCUUUUUUU	0.0145348	0.9679271	ibsD	7751628	toxic membrane protein

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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