Input and runtime details for job 8403881 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_000913
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 8403881 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:15:25 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:18:18 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:19:13 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:20:45 CET 2020
	Job Completed		@ Mon Jan 27 14:20:57 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=8403881&toolName=IntaRNA ( 30 days expiry )

Description of the job

RyhB - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
b1423	9	56	50	56	11.6	RyhB	38	84	38	44	14.89	-13.15	-39.64	-1.38269	CAGCAUCACGGCGGAUGAGAUUCGGGAACAGUUUUCGCAGGCAAUGUC&GACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUG	(((((((.((((((.((((.....((((((((........((((((((&))))))))....)))).)))).......)))).)).)))))))))))	AGUUUGUAAAUUCUUCCCGCAAGAGUGAAUGCGGUUACCUACACUCCAGAUUACUGACCACUGGAGGCAGACACUAUGGCGAACAGCAUCACGGCGGAUGAGAUUCGGGAACAGUUUUCGCAGGCAAUGUCAGCCAUGUACCAGCAAGAA	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0088035	0.9291058	ydcJ	945991	putative metalloenzyme
b2799	-75	-65	-71	-65	1.36	RyhB	56	66	56	62	2	-13.09	-16.45	-1.37638	GUAGUGCUGGA&UCCAGUAUUAC	(((((((((((&)))))))))))	GUAGUGCUGGAGAAAUUCAAAACCUAUGGGUUACGAAUUGAAGAGUAAUUUCGUAAAGCAACAAGGAGAAGGAUGAUGGCUAACAGAAUGAUUCUGAACGAAACGGCAUGGUUUGGUCGGGGUGCUGUUGGGGCUUUAACCGAUGAGGUG	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0091019	0.9291058	fucO	947273	L-12-propanediol oxidoreductase
b4355	-74	-65	-73	-67	2.24	RyhB	16	25	18	24	4.34	-13.09	-19.67	-1.37638	UCUCCGCGGG&CUCGCGGAGA	((((((((((&))))))))))	AUCUCCGCGGGAUAUUCAUAAAGUUUUUCCUUUCCAGGCCGAAAAUCUUGCAUCGGUCCACAGGAAAGAGAAACCAUGUUAAAACGUAUCAAAAUUGUGACCAGCUUACUGCUGGUUUUGGCCGUUUUUGGCCUUUUACAACUGACAUCA	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0091019	0.9291058	tsr	948884	methyl-accepting chemotaxis protein I serine sensor receptor
b3928	30	40	33	39	2.04	RyhB	51	61	52	58	2.88	-13.08	-18	-1.37533	ACUGGAAGCAA&UUGCUUCCAGU	(((((((((((&)))))))))))	UCGUAUUUAUUAAGGCGUCACCGGUAAUCGGGACGAGGAUUUUUAUCCAUCAACGCCUUGCAAUUCAGGAGAGGUAUGACAAUGUCAUUAGAAGUGUUUGAGAAACUGGAAGCAAAAGUACAGCAGGCGAUUGAUACCAUCACUCUGUUG	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0091526	0.9291058	zapB	948420	FtsZ stabilizer septal ring assembly factor cell division stimulator
b2283	-15	-6	-13	-7	0.75	RyhB	52	61	53	59	2.88	-13.04	-16.67	-1.37112	ACUGGAAGCA&UGCUUCCAGU	((((((((((&))))))))))	ACCUGCUGAAAGAGCGCUGGUAACCGAAUUUCGAUUAACGCUCAGUCUCUGACUGAGAAAACUGGAAGCAUGCUAAUGGCUACAAUUCAUGUAGACGGCAAAGAAUACGAGGUCAACGGAGCGGACAACCUGCUGGAAGCUUGUCUGUCU	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0093579	0.9291058	nuoG	946762	NADH:ubiquinone oxidoreductase chain G
b0556	-10	11	1	7	5.95	RyhB	39	58	43	49	3.21	-12.89	-22.05	-1.35535	GGGGGCAACAGUGAGCAGAGU&ACAUUGCUCACAUUGCUUCC	((((((((..(((((((..((&))..))))))).))))))))	CGCUGGCGGUAAGCAAUGGAAAGGCCUGAUGACUCGUCGUGAGAUUGAGCGUGAAGUCUGUUUGUGGGGGCAACAGUGAGCAGAGUAACCGCGAUUAUAUCCGCUCUGAUUAUCUGCAUCAUCGUCAGCCUGUCAUGGGCGGUCAAUCAU	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0101674	0.9496255	rzpD	945929	DLP12 prophage putative murein endopeptidase
b2230	-27	3	-27	-21	8.92	RyhB	40	76	70	76	12.64	-12.71	-34.27	-1.33643	GGCUGGCUAUACACAAGGAAUGUGGCAAUG&CAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCC	((((((((((((...((.((((((((((((&)))))).)))))).))....)))).....))))))))	UUUUGUCGUAUUAGGGCACGGUAAAGUUUGGCUGUGCCCGUAAAAAAUGGCUGGCUAUACACAAGGAAUGUGGCAAUGAGUGGUGAAAAAAAGGCGAAAGGCUGGCGGUUCUAUGGUCUUGUAGGUUUUGGCGCAAUAGCACUGCUUUCC	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0112262	0.9496255	yfaA	946618	DUF2138 family protein putative host defense protein
b0896	51	69	52	58	3.7	RyhB	36	54	47	53	3.22	-12.7	-19.62	-1.33537	GCAAUGUGUAGCAGGUGGU&ACGACAUUGCUCACAUUGC	((((((((.((((.((.((&)).))..))))))))))))	UGAUCAAACCCAAUGCCAAUAGCCGCCCGACCGGGGAUACCACUGGCUAUCUGGCAAACCCGAAGGAGGUGUGAGAUGGGAAGUGGAUGGCAUGAAUGGCCGCUGAUGAUCUUCACGGUCUUCGGGCAAUGUGUAGCAGGUGGUUUUAUC	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.011288	0.9496255	dmsC	945502	dimethyl sulfoxide reductase anaerobic subunit C
b3248	19	35	29	35	5.01	RyhB	18	35	18	24	9.68	-12.53	-27.22	-1.3175	GCUUCCGGUUCUCCGCG&CGCGGAGAACCUGAAAGC	(((((.(((((((((((&))))))))))).)).)))	UGGGGUGCUCUGGCCGUGGAUUUUCUUGCUGAUGCGCAAAGUCCGUCAGCAGUUUGCAGUGCAAUAAAGGUUUCUAUGACUUCUCUGUAUUUAGCUUCCGGUUCUCCGCGUCGUCAGGAGUUACUUGCGCAACUUGGCGUGACCUUUGAA	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0123886	0.9496255	yhdE	947753	dTTP/UTP pyrophosphatase m(5)UTP/m(5)CTP/pseudo-UTP pyrophosphatase
b1044	63	73	66	72	2.88	RyhB	52	62	53	59	2.88	-12.49	-18.25	-1.31329	UGCUGGAAGCA&UGCUUCCAGUA	(((((((((((&)))))))))))	GCUGCGAAUCAGCCAACCUGAAAGUAUGGAUAACACAACCCUCAAGGAUGACUAAUCAUUGAGGAAAUAGAAUAAAUGUUCAGACCUUUUUUAAACUCUCUUAUGCUCGGCAGUUUGUUUUUUCCUUUUAUUGCCAUUGCUGGAAGCACC	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.012662	0.9496255	ymdA	949012	uncharacterized protein

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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