Input and runtime details for job 8403881 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_000913
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 8403881 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:15:25 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:18:18 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:19:13 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:20:45 CET 2020
	Job Completed		@ Mon Jan 27 14:20:57 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=8403881&toolName=IntaRNA ( 30 days expiry )

Description of the job

RyhB - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
b3010	-68	-29	-35	-29	12.31	RyhB	38	86	38	44	14.91	-11.97	-39.19	-1.25862	GCCAGCGCCCUGGCUCUCAAUGCUCAUUUCUGCCAAUGUC&GACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGC	(((((((((((((((...((((((((.......(((((((&))))))).......)).....))))))....)))))...))))))))))	UGUGAUCGCCAGCGCCCUGGCUCUCAAUGCUCAUUUCUGCCAAUGUCUUGCCUAUUUCUCCAGAGUGCUGGAGAAAUGCUACAAAAUUGCGCACAAUCAAAUUGCCGCAUUAUUCCUAAGAAAUUACGCGAUAUGAAACGUGAAGAGAUU	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0167745	0.9496255	yqhC	947491	transcriptional activator of yqhD
b3311	-68	-41	-49	-43	11.36	RyhB	37	60	39	45	3.3	-11.34	-26	-1.19237	CUGUUGAAGCAAGUGCGUCGCGAUGUCG&CGACAUUGCUCACAUUGCUUCCAG	(((..((((((((((....(((((((((&))))))))).))).))))))))))	GUCUCACCUGUUGAAGCAAGUGCGUCGCGAUGUCGCACGCGUUAAGACUUUACUGAACGAGAAGGCGGGUGCGUAAUGACCGAUAAAAUCCGUACUCUGCAAGGUCGCGUUGUUAGCGACAAAAUGGAGAAAUCCAUUGUUGUUGCUAUC	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0234421	0.9496255	rpsQ	947808	30S ribosomal subunit protein S17
b3543	-67	-48	-57	-51	2.65	RyhB	41	58	44	50	3.21	-12.11	-17.97	-1.27334	GGAAUUGAUUUGUGAGCAAU&AUUGCUCACAUUGCUUCC	((((......((((((((((&))))))))))....))))	UAAGGGCGGGAAUUGAUUUGUGAGCAAUACAGACACGCAGUUCCAGGCUGCGGGUCACUAUAGAGAAUCCGGGUUAUGUUGCAGUUUAUUCUCCGACGUUUGGGACUCGUCAUCCCCACGUUUAUCGGUAUUACCCUUCUCACAUUUGCC	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0155581	0.9496255	dppB	948063	dipeptide/heme ABC transporter permease
b3032	-67	-43	-49	-43	8.96	RyhB	40	63	40	46	3.3	-10.86	-23.12	-1.1419	AUACUGUUUAGCACAUGGAGCGAUG&CAUUGCUCACAUUGCUUCCAGUAU	((((((...((((.(((((((((((&)))))))).)))))))..))))))	UGGUUGCGAUACUGUUUAGCACAUGGAGCGAUGGCGAUUCCGGUUUAUUAGCGUCGUGAAACCUAAGGACACCAUUUGGAAAGCCUGUUAACCCUUCCUCUGGCUGGUGAGGCCAGAGUCAGGAUUUUACAAAUUACCGACACUCACCUG	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0301134	0.9496255	cpdA	947515	3'5' cAMP phosphodiesterase
b3492	-66	-42	-64	-58	5.23	RyhB	42	65	57	63	3.3	-10.96	-19.49	-1.15242	UGAUGCUGGUGCGACCACUGAGGAA&UUGCUCACAUUGCUUCCAGUAUUA	(((((((((.((((....((((.((&)).))))..))))..)))))))))	UAGUAAUAUUGAUGCUGGUGCGACCACUGAGGAAUCUUUACAAUUCACGCCCGUUUUUUCUAAGAGGAGCGCAACGUGGAAAGGUUUGAUGCCAUUAUUAUAGGCGCUGGUGCGGCGGGUAUGUUCUGUUCUGCGCUGGCAGGUCAGGCA	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.028592	0.9496255	yhiN	947996	putative oxidoreductase
b4255	-63	-43	-62	-56	5.2	RyhB	39	59	52	58	3.27	-18.09	-26.56	-1.90212	UGGAGGCAAUGUCAGUGGUGU&ACAUUGCUCACAUUGCUUCCA	((((((((((((.((((((((&)))))))).))))))))))))	UUGUCACAAAGGUGGAGGCAAUGUCAGUGGUGUGUGACAAUAAGAGUAUCGGCAGGACAUUAAGAGGAAUGAGCCAUGGCAAACCCGGAACAACUGGAAGAACAGCGUGAAGAAACACGUUUGAUUAUUGAAGAAUUACUGGAAGAUGGC	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0004554	0.3956288	rraB	948773	protein inhibitor of RNase E
b3080	-59	-34	-44	-38	3.58	RyhB	1	25	6	12	7.71	-14.07	-25.36	-1.47943	UCCCAGCGACCAAAUCUUCCUGAUGC&GCGAUCAGGAAGACCCUCGCGGAGA	((((.((((.....((((((((((((&)).))))))))))...)))))).))	AAAAUGCCUCUCACGAUCCCAGCGACCAAAUCUUCCUGAUGCAAUAUUUCUACUGGUAAUAAGGACUGUAAUUUCAUGAAAAUAAAAACUAUUUUAACGCCAGUAACCUGCGCUCUGCUGAUAAGUUUUUCCGCCCAUGCCGCUAACGCC	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0052402	0.9291058	ygjK	947596	alpha-glucosidase
b2155	-55	-41	-50	-44	3.08	RyhB	41	57	44	50	3.2	-11.36	-17.64	-1.19448	GAAGAUGUGAGCGAU&AUUGCUCACAUUGCUUC	(((((((((((((((&)))))))))))..))))	CGUACGCAAGGGGACGUGAAGAAGAUGUGAGCGAUAACCCAUUUUAUUUUCGUAGUUACCUCAUGGAGAUAUGGAAUGUUUAGGUUGAACCCUUUCGUACGGGUCGGGCUGUGUUUGUCCGCUAUUUCUUGUGCAUGGCCUGUGUUAGCG	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0231967	0.9496255	cirA	949042	colicin IA outer membrane receptor and translocator ferric iron-catecholate transporter
b1224	-50	-41	-48	-42	1.47	RyhB	37	46	38	44	1.15	-12.19	-14.81	-1.28175	AGCAAUGUCG&CGACAUUGCU	((((((((((&))))))))))	GAGCGUUACCUUGCCCUUAAACAUUAGCAAUGUCGAUUUAUCAGAGGGCCGACAGGCUCCCACAGGAGAAAACCGAUGAGUAAAUUCCUGGACCGGUUUCGCUACUUCAAACAGAAGGGUGAAACCUUUGCCGAUGGGCAUGGCCAGCUU	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0149008	0.9496255	narG	945782	nitrate reductase 1 alpha subunit
b1269	-46	8	2	8	15.9	RyhB	42	90	42	48	14.91	-11.89	-42.7	-1.2502	AAAAGCAUAUCAGACGCCUGGGAAGGCGACACCAAAAGGAAGCUCUAUGAGCGA&UUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	((((((....(((.(((((((...(((..........((((((....(((((((&)))))))....))))))...........)))..))))))))))))))))	CAGAUGUAAGUUCUGACUCACUGUUGCACAAAAGCAUAUCAGACGCCUGGGAAGGCGACACCAAAAGGAAGCUCUAUGAGCGAAAAGCUACAGAAAGUGCUGGCGCGUGCCGGCCACGGUUCUCGCCGUGAAAUCGAAUCUAUUAUUGAA	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0175092	0.9496255	rluB	945840	23S rRNA pseudouridine(2605) synthase

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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