Freiburg RNA Tools
MutaRNA - Results
BIF
IFF
MutaRNA 9510481

Input and runtime details for job 9510481 (precomputed example)

Sequence Parameters

? Wild type single RNA sequence in FASTA[.fa]
? Mutation encodingA101C

Folding options

? Window size (RNAplfold)200
? Maximal base pair span (RNAplfold)150

Job ID 9510481 (server version trunk)

?Job Submitted & Queued@ Tue Apr 14 17:50:58 CEST 2020
?MutaRNA Started@ Tue Apr 14 17:52:16 CEST 2020
?MutaRNA Finished & Post-Processing@ Tue Apr 14 17:57:58 CEST 2020
 DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=9510481&toolName=MutaRNA ( 30 days expiry )

Description of the job

Revisiting mutation A30C in KRAS gene, which is known to increase gene expression (Sharma et al., 2019). Note, here mutation with sequence context +-100nt, i.e. mutation is at position 101. The loss of the first hairpin close to the RBS might explain the increased translation efficiency.

Output

Download an archive file containing the complete results produced by the job in [.zip] format.

Results file content

cd /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/; RNAplfold -W 200 -L 150 -u 9 < /tmp/6963248.1.websrv1.q/tmpg16chxh5 b'' b'' snp_locs: [] cd "/scratch/rna/bisge001/Software/MutaRNA/1.3/bin/dot2circ/"; python dot2circ.py --outputdir '/scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/' --prefix 'RNA-WILDTYPE-circos' --dp-file '/scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA_dp.ps' --title 'RNA-WILDTYPE' tmpdir is: /tmp/6963248.1.websrv1.q/tmpfecflzjy/ ['/tmp/6963248.1.websrv1.q/tmpfecflzjy/data/sequence.txt', '/tmp/6963248.1.websrv1.q/tmpfecflzjy/data/circos.sequence.txt', '/tmp/6963248.1.websrv1.q/tmpfecflzjy/data/circos.karyotype.txt', '/tmp/6963248.1.websrv1.q/tmpfecflzjy/data/circos.bplinks.txt'] calling: " circos -outputdir /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/ -param image/file**="RNA-WILDTYPE-circos.png" -param image/radius*=1000p -param karyotype=/tmp/6963248.1.websrv1.q/tmpfecflzjy//data/circos.karyotype.txt -param highlights/highlight/file=/scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local//genes.formatted.txt -param links/link/file=/tmp/6963248.1.websrv1.q/tmpfecflzjy//data/circos.bplinks.txt -param ideogram/show_label=yes " ======circos output log======== debuggroup summary 3.38s welcome to circos v0.69-8 15 Jun 2019 on Perl 5.026002 debuggroup summary 3.42s current working directory /scratch/rna/bisge001/Software/MutaRNA/1.3/bin/dot2circ debuggroup summary 3.42s command /scratch/rna/bisge001/Software/conda-v4.6.0/envs/MutARNA-1.0/bin/circos -outputdir /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/ -param image/file**=RNA-WILDTYPE-circos.png -param image/radius*=1000p -param karyotype=/tmp/6963248.1.websrv1.q/tmpfecflzjy//data/circos.karyotype.txt -param highlights/highlight/file=/scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local//genes.formatted.txt -param links/link/file=/tmp/6963248.1.websrv1.q/tmpfecflzjy//data/circos.bplinks.txt -param ideogram/show_label=yes debuggroup summary 3.42s guessing configuration file debuggroup summary 3.46s found conf file /scratch/rna/bisge001/Software/MutaRNA/1.3/bin/dot2circ/circos.conf debuggroup summary 6.50s debug will appear for these features: output,summary debuggroup summary 6.52s bitmap output image /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-WILDTYPE-circos.png debuggroup summary 6.52s SVG output image /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-WILDTYPE-circos.svg debuggroup summary 6.60s parsing karyotype and organizing ideograms debuggroup summary 6.60s karyotype has 1 chromosomes of total size 202 debuggroup summary 6.60s applying global and local scaling debuggroup summary 6.60s allocating image, colors and brushes debuggroup summary 23.69s drawing 1 ideograms of total size 202 debuggroup summary 23.69s drawing highlights and ideograms debuggroup summary 23.76s found conf file /scratch/rna/bisge001/Software/conda-v4.6.0/envs/MutARNA-1.0/bin/../etc/tracks/highlight.bg.conf debuggroup summary 23.87s process track_0 highlight /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local//genes.formatted.txt debuggroup summary 24.09s found conf file /scratch/rna/bisge001/Software/conda-v4.6.0/envs/MutARNA-1.0/bin/../etc/tracks/link.conf debuggroup summary 24.13s process track_0 link /tmp/6963248.1.websrv1.q/tmpfecflzjy//data/circos.bplinks.txt debuggroup summary 24.72s drawing link track_0 z 50 debuggroup output 25.93s generating output debuggroup output 26.61s created PNG image /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-WILDTYPE-circos.png (291 kb) debuggroup output 26.78s created SVG image /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-WILDTYPE-circos.svg (246 kb) =============================== cd /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/; RNAplfold -W 200 -L 150 -u 9 < /tmp/6963248.1.websrv1.q/tmpge2uacky b'' b'' snp_locs: [101] cd "/scratch/rna/bisge001/Software/MutaRNA/1.3/bin/dot2circ/"; python dot2circ.py --outputdir '/scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/' --prefix 'RNA-MUTANT-circos' --dp-file '/scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-MUTANT_dp.ps' --title 'RNA-MUTANT' tmpdir is: /tmp/6963248.1.websrv1.q/tmp36q98xlr/ ['/tmp/6963248.1.websrv1.q/tmp36q98xlr/data/sequence.txt', '/tmp/6963248.1.websrv1.q/tmp36q98xlr/data/circos.sequence.txt', '/tmp/6963248.1.websrv1.q/tmp36q98xlr/data/circos.karyotype.txt', '/tmp/6963248.1.websrv1.q/tmp36q98xlr/data/circos.bplinks.txt'] calling: " circos -outputdir /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/ -param image/file**="RNA-MUTANT-circos.png" -param image/radius*=1000p -param karyotype=/tmp/6963248.1.websrv1.q/tmp36q98xlr//data/circos.karyotype.txt -param highlights/highlight/file=/scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local//genes.formatted.txt -param links/link/file=/tmp/6963248.1.websrv1.q/tmp36q98xlr//data/circos.bplinks.txt -param ideogram/show_label=yes " ======circos output log======== debuggroup summary 1.37s welcome to circos v0.69-8 15 Jun 2019 on Perl 5.026002 debuggroup summary 1.41s current working directory /scratch/rna/bisge001/Software/MutaRNA/1.3/bin/dot2circ debuggroup summary 1.41s command /scratch/rna/bisge001/Software/conda-v4.6.0/envs/MutARNA-1.0/bin/circos -outputdir /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/ -param image/file**=RNA-MUTANT-circos.png -param image/radius*=1000p -param karyotype=/tmp/6963248.1.websrv1.q/tmp36q98xlr//data/circos.karyotype.txt -param highlights/highlight/file=/scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local//genes.formatted.txt -param links/link/file=/tmp/6963248.1.websrv1.q/tmp36q98xlr//data/circos.bplinks.txt -param ideogram/show_label=yes debuggroup summary 1.41s guessing configuration file debuggroup summary 1.41s found conf file /scratch/rna/bisge001/Software/MutaRNA/1.3/bin/dot2circ/circos.conf debuggroup summary 2.22s debug will appear for these features: output,summary debuggroup summary 2.22s bitmap output image /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-MUTANT-circos.png debuggroup summary 2.22s SVG output image /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-MUTANT-circos.svg debuggroup summary 2.27s parsing karyotype and organizing ideograms debuggroup summary 2.28s karyotype has 1 chromosomes of total size 202 debuggroup summary 2.28s applying global and local scaling debuggroup summary 2.28s allocating image, colors and brushes debuggroup summary 5.86s drawing 1 ideograms of total size 202 debuggroup summary 5.86s drawing highlights and ideograms debuggroup summary 5.86s found conf file /scratch/rna/bisge001/Software/conda-v4.6.0/envs/MutARNA-1.0/bin/../etc/tracks/highlight.bg.conf debuggroup summary 5.89s process track_0 highlight /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local//genes.formatted.txt debuggroup summary 6.02s found conf file /scratch/rna/bisge001/Software/conda-v4.6.0/envs/MutARNA-1.0/bin/../etc/tracks/link.conf debuggroup summary 6.03s process track_0 link /tmp/6963248.1.websrv1.q/tmp36q98xlr//data/circos.bplinks.txt debuggroup summary 6.62s drawing link track_0 z 50 debuggroup output 7.81s generating output debuggroup output 8.62s created PNG image /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-MUTANT-circos.png (278 kb) debuggroup output 8.87s created SVG image /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-MUTANT-circos.svg (240 kb) =============================== min_max is [81, 140] cd "/scratch/rna/bisge001/Software/MutaRNA/1.3/bin/dot2circ/"; python dot2circ.py --outputdir '/scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/' --prefix 'RNA-MUTANT-weakened-circos' --dp-file '/scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-MUTANT_dp_diff_weakened.dp' --title 'RNA-MUTANT-weakened' tmpdir is: /tmp/6963248.1.websrv1.q/tmpj_79t54v/ ['/tmp/6963248.1.websrv1.q/tmpj_79t54v/data/sequence.txt', '/tmp/6963248.1.websrv1.q/tmpj_79t54v/data/circos.sequence.txt', '/tmp/6963248.1.websrv1.q/tmpj_79t54v/data/circos.karyotype.txt', '/tmp/6963248.1.websrv1.q/tmpj_79t54v/data/circos.bplinks.txt'] calling: " circos -outputdir /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/ -param image/file**="RNA-MUTANT-weakened-circos.png" -param image/radius*=1000p -param karyotype=/tmp/6963248.1.websrv1.q/tmpj_79t54v//data/circos.karyotype.txt -param highlights/highlight/file=/scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local//genes.formatted.txt -param links/link/file=/tmp/6963248.1.websrv1.q/tmpj_79t54v//data/circos.bplinks.txt -param ideogram/show_label=yes " ======circos output log======== debuggroup summary 1.32s welcome to circos v0.69-8 15 Jun 2019 on Perl 5.026002 debuggroup summary 1.36s current working directory /scratch/rna/bisge001/Software/MutaRNA/1.3/bin/dot2circ debuggroup summary 1.36s command /scratch/rna/bisge001/Software/conda-v4.6.0/envs/MutARNA-1.0/bin/circos -outputdir /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/ -param image/file**=RNA-MUTANT-weakened-circos.png -param image/radius*=1000p -param karyotype=/tmp/6963248.1.websrv1.q/tmpj_79t54v//data/circos.karyotype.txt -param highlights/highlight/file=/scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local//genes.formatted.txt -param links/link/file=/tmp/6963248.1.websrv1.q/tmpj_79t54v//data/circos.bplinks.txt -param ideogram/show_label=yes debuggroup summary 1.36s guessing configuration file debuggroup summary 1.36s found conf file /scratch/rna/bisge001/Software/MutaRNA/1.3/bin/dot2circ/circos.conf debuggroup summary 1.98s debug will appear for these features: output,summary debuggroup summary 1.98s bitmap output image /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-MUTANT-weakened-circos.png debuggroup summary 1.98s SVG output image /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-MUTANT-weakened-circos.svg debuggroup summary 2.01s parsing karyotype and organizing ideograms debuggroup summary 2.02s karyotype has 1 chromosomes of total size 202 debuggroup summary 2.02s applying global and local scaling debuggroup summary 2.02s allocating image, colors and brushes debuggroup summary 5.64s drawing 1 ideograms of total size 202 debuggroup summary 5.64s drawing highlights and ideograms debuggroup summary 5.64s found conf file /scratch/rna/bisge001/Software/conda-v4.6.0/envs/MutARNA-1.0/bin/../etc/tracks/highlight.bg.conf debuggroup summary 5.68s process track_0 highlight /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local//genes.formatted.txt debuggroup summary 5.82s found conf file /scratch/rna/bisge001/Software/conda-v4.6.0/envs/MutARNA-1.0/bin/../etc/tracks/link.conf debuggroup summary 5.83s process track_0 link /tmp/6963248.1.websrv1.q/tmpj_79t54v//data/circos.bplinks.txt debuggroup summary 6.04s drawing link track_0 z 50 debuggroup output 6.46s generating output debuggroup output 7.11s created PNG image /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-MUTANT-weakened-circos.png (180 kb) debuggroup output 7.25s created SVG image /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-MUTANT-weakened-circos.svg (90 kb) =============================== cd "/scratch/rna/bisge001/Software/MutaRNA/1.3/bin/dot2circ/"; python dot2circ.py --outputdir '/scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/' --prefix 'RNA-MUTANT-increased-circos' --dp-file '/scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-MUTANT_dp_diff_increased.dp' --title 'RNA-MUTANT-increased' tmpdir is: /tmp/6963248.1.websrv1.q/tmp8aq5n3_h/ ['/tmp/6963248.1.websrv1.q/tmp8aq5n3_h/data/sequence.txt', '/tmp/6963248.1.websrv1.q/tmp8aq5n3_h/data/circos.sequence.txt', '/tmp/6963248.1.websrv1.q/tmp8aq5n3_h/data/circos.karyotype.txt', '/tmp/6963248.1.websrv1.q/tmp8aq5n3_h/data/circos.bplinks.txt'] calling: " circos -outputdir /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/ -param image/file**="RNA-MUTANT-increased-circos.png" -param image/radius*=1000p -param karyotype=/tmp/6963248.1.websrv1.q/tmp8aq5n3_h//data/circos.karyotype.txt -param highlights/highlight/file=/scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local//genes.formatted.txt -param links/link/file=/tmp/6963248.1.websrv1.q/tmp8aq5n3_h//data/circos.bplinks.txt -param ideogram/show_label=yes " ======circos output log======== debuggroup summary 2.43s welcome to circos v0.69-8 15 Jun 2019 on Perl 5.026002 debuggroup summary 2.47s current working directory /scratch/rna/bisge001/Software/MutaRNA/1.3/bin/dot2circ debuggroup summary 2.47s command /scratch/rna/bisge001/Software/conda-v4.6.0/envs/MutARNA-1.0/bin/circos -outputdir /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/ -param image/file**=RNA-MUTANT-increased-circos.png -param image/radius*=1000p -param karyotype=/tmp/6963248.1.websrv1.q/tmp8aq5n3_h//data/circos.karyotype.txt -param highlights/highlight/file=/scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local//genes.formatted.txt -param links/link/file=/tmp/6963248.1.websrv1.q/tmp8aq5n3_h//data/circos.bplinks.txt -param ideogram/show_label=yes debuggroup summary 2.47s guessing configuration file debuggroup summary 2.47s found conf file /scratch/rna/bisge001/Software/MutaRNA/1.3/bin/dot2circ/circos.conf debuggroup summary 3.70s debug will appear for these features: output,summary debuggroup summary 3.71s bitmap output image /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-MUTANT-increased-circos.png debuggroup summary 3.71s SVG output image /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-MUTANT-increased-circos.svg debuggroup summary 3.78s parsing karyotype and organizing ideograms debuggroup summary 3.78s karyotype has 1 chromosomes of total size 202 debuggroup summary 3.78s applying global and local scaling debuggroup summary 3.78s allocating image, colors and brushes debuggroup summary 7.84s drawing 1 ideograms of total size 202 debuggroup summary 7.84s drawing highlights and ideograms debuggroup summary 7.84s found conf file /scratch/rna/bisge001/Software/conda-v4.6.0/envs/MutARNA-1.0/bin/../etc/tracks/highlight.bg.conf debuggroup summary 7.88s process track_0 highlight /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local//genes.formatted.txt debuggroup summary 8.01s found conf file /scratch/rna/bisge001/Software/conda-v4.6.0/envs/MutARNA-1.0/bin/../etc/tracks/link.conf debuggroup summary 8.03s process track_0 link /tmp/6963248.1.websrv1.q/tmp8aq5n3_h//data/circos.bplinks.txt debuggroup summary 8.25s drawing link track_0 z 50 debuggroup output 8.71s generating output debuggroup output 9.40s created PNG image /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-MUTANT-increased-circos.png (193 kb) debuggroup output 9.53s created SVG image /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/local/RNA-MUTANT-increased-circos.svg (96 kb) =============================== $(which remuRNA) /tmp/6963248.1.websrv1.q/tmpamokriwa.fa -p=1 ['SNP', 'H(wt||mu)', 'MFE(mu)', 'MFE(wt)', 'dMFE'] -m 1 SNP w Slen GC interval d_max p-value interval r_min p-value A101C 200 201 0.3383 86-139 0.4163 0.0104 85-135 -0.1571 0.0417 ['SNP', 'interval', 'd_max', 'p-value'] -m 2 SNP w Slen GC max_k d_max p-value interval d p-value A101C 200 201 0.3383 96 17.9079 0.0028 96-139 0.4243 0.0020 ['SNP', 'interval', 'd', 'p-value'] SNP scores were saved to: /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/remuRNA.csv /scratch/rna/bisge001/RNA_results/CARNA-result/MutaRNA_9510481/RNAsnp.csv

When using MutaRNA please cite :

Results are computed with MutaRNA version 1.3.0 (using RNAplfold 2.4.14, remuRNA 1.0, RNAsnp 1.2)