RaSE uses the graph vectorization technique of EDeN to compute a score indicative of the structural stability responsibility of each nucleotide in an RNA sequence. The score is computed as the similarity of the structure obtained by changing a single nucleotide with respect to the original structure. The structure is based on RNAplfold base pair probabilities and thus reflects the overall structural ensemble accessible to the RNA molecule. Per sequence position, only the mutation that yields the largest structural difference is reported.
RaSE - Point mutation evaluator
Results are computed with RaSE version 1.0.0 using VRNA 2.2.10 and EDeN 2.0