RaSE uses the graph vectorization technique of EDeN to compute a score indicative of the structural stability responsibility of each nucleotide in an RNA sequence. The score is computed as the similarity of the structure obtained by changing a single nucleotide with respect to the original structure. The structure is based on RNAplfold base pair probabilities and thus reflects the overall structural ensemble accessible to the RNA molecule. Per sequence position, only the mutation that yields the largest structural difference is reported. Its source code is available on github: https://github.com/fabriziocosta/RaSE-RNA-Structural-Stability-Estimator.

• Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
  Freiburg RNA tools: a central online resource for RNA-focused research and teaching
  Nucleic Acids Research, 46(W1), W25-W29, 2018.
  

Results are computed with RaSE version offline

Due to software incompatibilities, we had to decommission the RaSE webserver. The tool's source code is available on github: https://github.com/fabriziocosta/RaSE-RNA-Structural-Stability-Estimator.