Input and runtime details for job 3202356 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3202356 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:02 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:24:35 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:03:47 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:03:55 CET 2018
	Job Completed		@ Sat Feb 17 02:04:28 CET 2018

DIRECT ACCESS: https://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3202356&toolName=CopraRNA ( 30 days expiry )

Description of the job

GcvB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
24	0.000609	0.08814	stm3510	yhgH	-18.34	0.033208	106 -- 153	42 -- 84	DNA utilization protein GntX	_____________105____________________________________154\n_____________\|______________________________________\|\n5'-CCG...UCCGU____________G__G___AUUUCCCUC__________UGCCA...GCG-3'\n______________GCAGCAGCACAA_AU_ACA_________GCCGUAGGUC\n______________:\|\|:\|\|:\|\|\|\|\|_\|\|_\|\|\|_________:\|\|\|\|\|:\|:\|\n______________UGUUGUUGUGUU_UA_UGU_________UGGCAUUCGG\n3'-UUU...GUUUG____________G__G______________________UUUUC...UCA-5'\n_____________\|______________________________________\|\n_____________85_____________________________________41\n	135 -- 141	58 -- 64	-35.7194 kcal/mol	10.0869 kcal/mol	11.5136 kcal/mol	105 154@LB@ \| \|@LB@5'-CCG...UCCGU G G AUUUCCCUC UGCCA...GCG-3'@LB@ GCAGCAGCACAA AU ACA GCCGUAGGUC@LB@ :\|\|:\|\|:\|\|\|\|\| \|\| \|\|\| :\|\|\|\|\|:\|:\|@LB@ UGUUGUUGUGUU UA UGU UGGCAUUCGG@LB@3'-UUU...GUUUG G G UUUUC...UCA-5'@LB@ \| \|@LB@ 85 41@LB@@LB@@LB@Energy -18.34 kcal/mol@LB@Hybridization Energy -35.7194 kcal/mol@LB@Unfolding Energy - mRNA 10.0869 kcal/mol@LB@Unfolding Energy - sRNA 11.5136 kcal/mol@LB@@LB@Position - mRNA 106 -- 153@LB@Position - sRNA 42 -- 84@LB@Position Seed - mRNA 135 -- 141@LB@Position Seed - sRNA 58 -- 64@LB@
195	0.01085	0.197	stm0549	fimZ	-13.17	0.226725	219 -- 235	155 -- 171	DNA-binding response regulator	_____________218___________236\n_____________\|_____________\|\n5'-CAG...CUGUU_____________AACAC...UGA-3'\n______________AUCAUUAUGGACG\n______________\|\|\|\|:\|\|\|\|\|\|\|:\n______________UAGUGAUACCUGU\n3'-UUU...GUAAU_____________CUGUC...UCA-5'\n_____________\|_____________\|\n_____________172___________154\n	225 -- 231	159 -- 165	-17.3187 kcal/mol	3.33371 kcal/mol	3.42647 kcal/mol	218 236@LB@ \| \|@LB@5'-CAG...CUGUU AACAC...UGA-3'@LB@ AUCAUUAUGGACG@LB@ \|\|\|\|:\|\|\|\|\|\|\|:@LB@ UAGUGAUACCUGU@LB@3'-UUU...GUAAU CUGUC...UCA-5'@LB@ \| \|@LB@ 172 154@LB@@LB@@LB@Energy -13.17 kcal/mol@LB@Hybridization Energy -17.3187 kcal/mol@LB@Unfolding Energy - mRNA 3.33371 kcal/mol@LB@Unfolding Energy - sRNA 3.42647 kcal/mol@LB@@LB@Position - mRNA 219 -- 235@LB@Position - sRNA 155 -- 171@LB@Position Seed - mRNA 225 -- 231@LB@Position Seed - sRNA 159 -- 165@LB@
198	0.01098	0.197	stm2803	N/A	-14.51	0.140962	192 -- 262	36 -- 101	DNA-binding transcriptional regulator	_____________191___________________________263\n_____________\|_____________________________\|\n5'-GCU...AAAAA________UA___C_____UAU_______UCGCU...CGC-3'\n______________GCAGACCA__UGC__GCGC___CAGCACA\n______________\|\|\|\|\|\|\|\|__\|\|\|__:\|:\|___\|\|:\|\|\|\|\n______________CGUCUGGU__ACG__UGUG___GUUGUGU\n3'-UUU...CUUAG________UA___UU____UU________UGUAG...UCA-5'\n_____________\|_____________________________\|\n_____________102___________________________35\n	213 -- 219	74 -- 80	-31.0005 kcal/mol	8.41541 kcal/mol	13.1066 kcal/mol	191 263@LB@ \| \|@LB@5'-GCU...AAAAA UA C UAU UCGCU...CGC-3'@LB@ GCAGACCA UGC GCGC CAGCACA@LB@ \|\|\|\|\|\|\|\| \|\|\| :\|:\| \|\|:\|\|\|\|@LB@ CGUCUGGU ACG UGUG GUUGUGU@LB@3'-UUU...CUUAG UA UU UU UGUAG...UCA-5'@LB@ \| \|@LB@ 102 35@LB@@LB@@LB@Energy -14.51 kcal/mol@LB@Hybridization Energy -31.0005 kcal/mol@LB@Unfolding Energy - mRNA 8.41541 kcal/mol@LB@Unfolding Energy - sRNA 13.1066 kcal/mol@LB@@LB@Position - mRNA 192 -- 262@LB@Position - sRNA 36 -- 101@LB@Position Seed - mRNA 213 -- 219@LB@Position Seed - sRNA 74 -- 80@LB@
170	0.009347	0.1945	stm0477	acrR	-17.70	0.042454	229 -- 246	74 -- 91	DNA-binding transcriptional repressor AcrR	_____________228________________247\n_____________\|__________________\|\n5'-AAG...AGCGC__G_______________UCCUG...CGC-3'\n______________UG_AGACACGACAACACA\n______________\|\|_\|:\|\|\|\|:\|\|\|\|\|\|\|\|\n______________AC_UUUGUGUUGUUGUGU\n3'-UUU...GGUUA__G_______________UGUAG...UCA-5'\n_____________\|__________________\|\n_____________92_________________73\n	240 -- 246	74 -- 80	-24.9092 kcal/mol	3.16809 kcal/mol	3.73631 kcal/mol	228 247@LB@ \| \|@LB@5'-AAG...AGCGC G UCCUG...CGC-3'@LB@ UG AGACACGACAACACA@LB@ \|\| \|:\|\|\|\|:\|\|\|\|\|\|\|\|@LB@ AC UUUGUGUUGUUGUGU@LB@3'-UUU...GGUUA G UGUAG...UCA-5'@LB@ \| \|@LB@ 92 73@LB@@LB@@LB@Energy -17.70 kcal/mol@LB@Hybridization Energy -24.9092 kcal/mol@LB@Unfolding Energy - mRNA 3.16809 kcal/mol@LB@Unfolding Energy - sRNA 3.73631 kcal/mol@LB@@LB@Position - mRNA 229 -- 246@LB@Position - sRNA 74 -- 91@LB@Position Seed - mRNA 240 -- 246@LB@Position Seed - sRNA 74 -- 80@LB@
192	0.01067	0.197	stm2650	yfiP	-13.22	0.223534	188 -- 232	36 -- 90	DTW domain-containing protein	_____________187_________________________233\n_____________\|___________________________\|\n5'-UCA...UCUCC________UUUCUAUGACC________UGUCC...GCU-3'\n______________GCAAACGC___________AAUAACGC\n______________\|\|\|\|\|\|:\|___________\|\|:\|\|\|:\|\n______________CGUUUGUG___________UUGUUGUG\n3'-UUU...GUUAA___________________________UUGUA...UCA-5'\n_____________\|___________________________\|\n_____________91__________________________35\n	208 -- 214	75 -- 81	-16.7007 kcal/mol	3.95203 kcal/mol	3.72363 kcal/mol	187 233@LB@ \| \|@LB@5'-UCA...UCUCC UUUCUAUGACC UGUCC...GCU-3'@LB@ GCAAACGC AAUAACGC@LB@ \|\|\|\|\|\|:\| \|\|:\|\|\|:\|@LB@ CGUUUGUG UUGUUGUG@LB@3'-UUU...GUUAA UUGUA...UCA-5'@LB@ \| \|@LB@ 91 35@LB@@LB@@LB@Energy -13.22 kcal/mol@LB@Hybridization Energy -16.7007 kcal/mol@LB@Unfolding Energy - mRNA 3.95203 kcal/mol@LB@Unfolding Energy - sRNA 3.72363 kcal/mol@LB@@LB@Position - mRNA 188 -- 232@LB@Position - sRNA 36 -- 90@LB@Position Seed - mRNA 208 -- 214@LB@Position Seed - sRNA 75 -- 81@LB@
29	0.0007392	0.08814	stm3096	yqgE	-16.45	0.068552	198 -- 251	37 -- 84	DUF179 domain-containing protein	_____________197___________252\n_____________\|_____________\|\n5'-UGG...UAUAU_____________CAGGA...GCG-3'\n______________UUGCGAGCACAAC\n______________\|\|\|\|:\|:\|\|\|\|\|\|\n______________AACGUUUGUGUUG\n3'-UUU...UGGUU_____________UUGUG...UCA-5'\n_____________\|_____________\|\n_____________85____________36\n	284 -- 290	80 -- 86	-18.5161 kcal/mol	2.82597 kcal/mol	3.5889 kcal/mol	197 252@LB@ \| \|@LB@5'-UGG...UAUAU CAGGA...GCG-3'@LB@ UUGCGAGCACAAC@LB@ \|\|\|\|:\|:\|\|\|\|\|\|@LB@ AACGUUUGUGUUG@LB@3'-UUU...UGGUU UUGUG...UCA-5'@LB@ \| \|@LB@ 85 36@LB@@LB@@LB@Energy -16.45 kcal/mol@LB@Hybridization Energy -18.5161 kcal/mol@LB@Unfolding Energy - mRNA 2.82597 kcal/mol@LB@Unfolding Energy - sRNA 3.5889 kcal/mol@LB@@LB@Position - mRNA 198 -- 251@LB@Position - sRNA 37 -- 84@LB@Position Seed - mRNA 284 -- 290@LB@Position Seed - sRNA 80 -- 86@LB@
48	0.001762	0.1291	stm4523	yjiW	-20.55	0.014078	157 -- 299	40 -- 186	endoribonuclease SymE	_____________156____________________________________________________________________________________________300\n_____________\|______________________________________________________________________________________________\|\n5'-GUG...AUUUU____AA___CCA__A__A_______CCAUUCU__________CG______UC__GAAGUCUCCC___________C______CUU_________UAUGC...AUU-3'\n______________AGGG__UAG___UC_UG_CUACCGU_______AUUGCAGAUC___UGCGA__CA__________CAACAA_AUAA_CGUCAC___ACCGUGAGU\n______________\|\|\|\|__\|\|\|___\|\|_:\|_\|\|\|\|\|\|\|_______\|\|:\|\|\|\|\|:\|___\|\|\|:\|__\|\|__________\|\|\|\|\|\|_\|:\|\|_\|:\|\|\|\|___\|\|\|\|\|:\|\|:\n______________UCCC__AUC___AG_GC_GAUGGCA_______UAGCGUCUGG___ACGUU__GU__________GUUGUU_UGUU_GUAGUG___UGGCAUUCG\n3'-UUU...CUUUU______________A__________CCAGACU__________UUA_____U___________________G___________U___________GUUUU...UCA-5'\n_____________\|______________________________________________________________________________________________\|\n_____________187____________________________________________________________________________________________39\n	222 -- 228	95 -- 101	-59.8396 kcal/mol	18.2388 kcal/mol	27.0823 kcal/mol	156 300@LB@ \| \|@LB@5'-GUG...AUUUU AA CCA A A CCAUUCU CG UC GAAGUCUCCC C CUU UAUGC...AUU-3'@LB@ AGGG UAG UC UG CUACCGU AUUGCAGAUC UGCGA CA CAACAA AUAA CGUCAC ACCGUGAGU@LB@ \|\|\|\| \|\|\| \|\| :\| \|\|\|\|\|\|\| \|\|:\|\|\|\|\|:\| \|\|\|:\| \|\| \|\|\|\|\|\| \|:\|\| \|:\|\|\|\| \|\|\|\|\|:\|\|:@LB@ UCCC AUC AG GC GAUGGCA UAGCGUCUGG ACGUU GU GUUGUU UGUU GUAGUG UGGCAUUCG@LB@3'-UUU...CUUUU A CCAGACU UUA U G U GUUUU...UCA-5'@LB@ \| \|@LB@ 187 39@LB@@LB@@LB@Energy -20.55 kcal/mol@LB@Hybridization Energy -59.8396 kcal/mol@LB@Unfolding Energy - mRNA 18.2388 kcal/mol@LB@Unfolding Energy - sRNA 27.0823 kcal/mol@LB@@LB@Position - mRNA 157 -- 299@LB@Position - sRNA 40 -- 186@LB@Position Seed - mRNA 222 -- 228@LB@Position Seed - sRNA 95 -- 101@LB@
185	0.01043	0.1967	stm1700	fabI	-13.03	0.238331	75 -- 107	38 -- 76	enoyl-[acyl-carrier-protein] reductase	_____________74______________________________108\n_____________\|_______________________________\|\n5'-AGU...AGCGU____C______C___________________UAUAA...CUG-3'\n______________GACA_ACGGGG________CA_GGAGUGGGA\n______________\|\|\|\|_\|\|:\|\|\|________\|\|_\|:\|\|\|\|::\|\n______________CUGU_UGUCCC________GU_CUUCACUUU\n3'-UUU...GAUAC____C______AUUUACAU__C_________UUCCC...UCA-5'\n_____________\|_______________________________\|\n_____________77______________________________37\n	96 -- 102	134 -- 140	-20.8604 kcal/mol	6.05685 kcal/mol	6.0649 kcal/mol	74 108@LB@ \| \|@LB@5'-AGU...AGCGU C C UAUAA...CUG-3'@LB@ GACA ACGGGG CA GGAGUGGGA@LB@ \|\|\|\| \|\|:\|\|\| \|\| \|:\|\|\|\|::\|@LB@ CUGU UGUCCC GU CUUCACUUU@LB@3'-UUU...GAUAC C AUUUACAU C UUCCC...UCA-5'@LB@ \| \|@LB@ 77 37@LB@@LB@@LB@Energy -13.03 kcal/mol@LB@Hybridization Energy -20.8604 kcal/mol@LB@Unfolding Energy - mRNA 6.05685 kcal/mol@LB@Unfolding Energy - sRNA 6.0649 kcal/mol@LB@@LB@Position - mRNA 75 -- 107@LB@Position - sRNA 38 -- 76@LB@Position Seed - mRNA 96 -- 102@LB@Position Seed - sRNA 134 -- 140@LB@
87	0.004009	0.1596	stm2067	sbcB	-11.70	0.367652	220 -- 292	35 -- 90	exodeoxyribonuclease I	_____________219_____________________293\n_____________\|_______________________\|\n5'-AUU...AUGUA______U________________AUUGG...CCG-3'\n______________CGAACA_UAGCA__ACAAUAUCA\n______________\|:\|\|\|\|_:\|:\|\|__\|\|\|\|:\|\|\|\|\n______________GUUUGU_GUUGU__UGUUGUAGU\n3'-UUU...UUAAC____________UG_________GUUGG...UCA-5'\n_____________\|_______________________\|\n_____________91______________________34\n	110 -- 116	68 -- 74	-17.8808 kcal/mol	2.08887 kcal/mol	5.27986 kcal/mol	219 293@LB@ \| \|@LB@5'-AUU...AUGUA U AUUGG...CCG-3'@LB@ CGAACA UAGCA ACAAUAUCA@LB@ \|:\|\|\|\| :\|:\|\| \|\|\|\|:\|\|\|\|@LB@ GUUUGU GUUGU UGUUGUAGU@LB@3'-UUU...UUAAC UG GUUGG...UCA-5'@LB@ \| \|@LB@ 91 34@LB@@LB@@LB@Energy -11.70 kcal/mol@LB@Hybridization Energy -17.8808 kcal/mol@LB@Unfolding Energy - mRNA 2.08887 kcal/mol@LB@Unfolding Energy - sRNA 5.27986 kcal/mol@LB@@LB@Position - mRNA 220 -- 292@LB@Position - sRNA 35 -- 90@LB@Position Seed - mRNA 110 -- 116@LB@Position Seed - sRNA 68 -- 74@LB@
153	0.008123	0.1845	stm2512	xseA	-14.57	0.138273	215 -- 252	42 -- 82	exodeoxyribonuclease VII large subunit	_____________214________253\n_____________\|__________\|\n5'-CGU...UUUUU__________GCCUU...GCG-3'\n______________ACCGUAAGCC\n______________\|\|\|\|\|\|\|\|\|\|\n______________UGGCAUUCGG\n3'-UUU...AGUGU__________UUUUC...UCA-5'\n_____________\|__________\|\n_____________83_________41\n	234 -- 240	56 -- 62	-17.1871 kcal/mol	1.1981 kcal/mol	7.48121 kcal/mol	214 253@LB@ \| \|@LB@5'-CGU...UUUUU GCCUU...GCG-3'@LB@ ACCGUAAGCC@LB@ \|\|\|\|\|\|\|\|\|\|@LB@ UGGCAUUCGG@LB@3'-UUU...AGUGU UUUUC...UCA-5'@LB@ \| \|@LB@ 83 41@LB@@LB@@LB@Energy -14.57 kcal/mol@LB@Hybridization Energy -17.1871 kcal/mol@LB@Unfolding Energy - mRNA 1.1981 kcal/mol@LB@Unfolding Energy - sRNA 7.48121 kcal/mol@LB@@LB@Position - mRNA 215 -- 252@LB@Position - sRNA 42 -- 82@LB@Position Seed - mRNA 234 -- 240@LB@Position Seed - sRNA 56 -- 62@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching