Input and runtime details for job 3202356 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3202356 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:02 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:24:35 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:03:47 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:03:55 CET 2018
	Job Completed		@ Sat Feb 17 02:04:28 CET 2018

DIRECT ACCESS: https://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3202356&toolName=CopraRNA ( 30 days expiry )

Description of the job

GcvB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
193	0.01075	0.197	stm3734	rph	-15.48	0.098722	165 -- 206	34 -- 78	ribonuclease PH	_____________164____________________________207\n_____________\|______________________________\|\n5'-GCG...AUAAU_____________U___UUU__________AUCUC...GAG-3'\n______________GCGCAGC__CACA_UCG___UAAGCCGGAG\n______________:\|:\|\|:\|__\|\|\|\|_:\|\|___\|\|\|\|\|\|::\|:\n______________UGUGUUG__GUGU_GGC___AUUCGGUUUU\n3'-UUU...GUUGU_______UA____U________________CGGGU...UCA-5'\n_____________\|______________________________\|\n_____________79_____________________________33\n	183 -- 189	55 -- 61	-24.9708 kcal/mol	4.11178 kcal/mol	11.3295 kcal/mol	164 207@LB@ \| \|@LB@5'-GCG...AUAAU U UUU AUCUC...GAG-3'@LB@ GCGCAGC CACA UCG UAAGCCGGAG@LB@ :\|:\|\|:\| \|\|\|\| :\|\| \|\|\|\|\|\|::\|:@LB@ UGUGUUG GUGU GGC AUUCGGUUUU@LB@3'-UUU...GUUGU UA U CGGGU...UCA-5'@LB@ \| \|@LB@ 79 33@LB@@LB@@LB@Energy -15.48 kcal/mol@LB@Hybridization Energy -24.9708 kcal/mol@LB@Unfolding Energy - mRNA 4.11178 kcal/mol@LB@Unfolding Energy - sRNA 11.3295 kcal/mol@LB@@LB@Position - mRNA 165 -- 206@LB@Position - sRNA 34 -- 78@LB@Position Seed - mRNA 183 -- 189@LB@Position Seed - sRNA 55 -- 61@LB@
180	0.009911	0.1961	stm0199	stfF	-15.44	0.100302	89 -- 133	44 -- 87	fimbrial protein	_____________88________________134\n_____________\|_________________\|\n5'-ACC...AUUUC___________G_____GGUCC...AUU-3'\n______________AAUACAGCGGC_GCAGC\n______________\|\|:\|\|\|:\|::\|_:\|\|:\|\n______________UUGUGUUGUUG_UGUUG\n3'-UUU...AACGU_________________UAGUG...UCA-5'\n_____________\|_________________\|\n_____________88________________43\n	93 -- 99	77 -- 83	-16.9774 kcal/mol	5.80505 kcal/mol	3.59664 kcal/mol	88 134@LB@ \| \|@LB@5'-ACC...AUUUC G GGUCC...AUU-3'@LB@ AAUACAGCGGC GCAGC@LB@ \|\|:\|\|\|:\|::\| :\|\|:\|@LB@ UUGUGUUGUUG UGUUG@LB@3'-UUU...AACGU UAGUG...UCA-5'@LB@ \| \|@LB@ 88 43@LB@@LB@@LB@Energy -15.44 kcal/mol@LB@Hybridization Energy -16.9774 kcal/mol@LB@Unfolding Energy - mRNA 5.80505 kcal/mol@LB@Unfolding Energy - sRNA 3.59664 kcal/mol@LB@@LB@Position - mRNA 89 -- 133@LB@Position - sRNA 44 -- 87@LB@Position Seed - mRNA 93 -- 99@LB@Position Seed - sRNA 77 -- 83@LB@
150	0.007945	0.1845	stm2555	glyA	-15.42	0.100951	252 -- 282	137 -- 168	serine hydroxymethyltransferase	_____________251_________________________________283\n_____________\|___________________________________\|\n5'-CAC...GGCAG________________AA________GUC______AGCAC...UCG-3'\n______________GCUAUGGA___GCAGG__AAA_GUAC___AGGAAG\n______________:\|\|\|\|\|\|\|___:\|\|\|\|__\|\|\|_\|\|\|\|___\|\|\|\|\|\|\n______________UGAUACCU___UGUCC__UUU_CAUG___UCCUUC\n3'-UUU...AUUAG________GUC_____CA___A_____________ACUUU...UCA-5'\n_____________\|___________________________________\|\n_____________169_________________________________136\n	253 -- 259	161 -- 167	-28.2568 kcal/mol	5.17684 kcal/mol	6.91101 kcal/mol	251 283@LB@ \| \|@LB@5'-CAC...GGCAG AA GUC AGCAC...UCG-3'@LB@ GCUAUGGA GCAGG AAA GUAC AGGAAG@LB@ :\|\|\|\|\|\|\| :\|\|\|\| \|\|\| \|\|\|\| \|\|\|\|\|\|@LB@ UGAUACCU UGUCC UUU CAUG UCCUUC@LB@3'-UUU...AUUAG GUC CA A ACUUU...UCA-5'@LB@ \| \|@LB@ 169 136@LB@@LB@@LB@Energy -15.42 kcal/mol@LB@Hybridization Energy -28.2568 kcal/mol@LB@Unfolding Energy - mRNA 5.17684 kcal/mol@LB@Unfolding Energy - sRNA 6.91101 kcal/mol@LB@@LB@Position - mRNA 252 -- 282@LB@Position - sRNA 137 -- 168@LB@Position Seed - mRNA 253 -- 259@LB@Position Seed - sRNA 161 -- 167@LB@
50	0.001812	0.1291	stm3207	ygiH	-15.41	0.101358	70 -- 101	63 -- 93	acyl-phosphate--glycerol-3-phosphate O-acyltransferase	_____________69___________________________________102\n_____________\|____________________________________\|\n5'-UAU...CGAAA_____UCUAU__________U_______________UGCCU...CGC-3'\n______________GUUGC_____AAAUACAAUA_C____CAUCACGACC\n______________:\|\|\|\|_____\|\|\|:\|\|\|\|:\|_\|____\|\|\|\|\|\|:\|\|\|\n______________UAACG_____UUUGUGUUGU_G____GUAGUGUUGG\n3'-UUU...CUGGU____________________U_UGUU__________CAUUC...UCA-5'\n_____________\|____________________________________\|\n_____________94___________________________________62\n	95 -- 101	63 -- 69	-25.69 kcal/mol	1.94006 kcal/mol	8.62086 kcal/mol	69 102@LB@ \| \|@LB@5'-UAU...CGAAA UCUAU U UGCCU...CGC-3'@LB@ GUUGC AAAUACAAUA C CAUCACGACC@LB@ :\|\|\|\| \|\|\|:\|\|\|\|:\| \| \|\|\|\|\|\|:\|\|\|@LB@ UAACG UUUGUGUUGU G GUAGUGUUGG@LB@3'-UUU...CUGGU U UGUU CAUUC...UCA-5'@LB@ \| \|@LB@ 94 62@LB@@LB@@LB@Energy -15.41 kcal/mol@LB@Hybridization Energy -25.69 kcal/mol@LB@Unfolding Energy - mRNA 1.94006 kcal/mol@LB@Unfolding Energy - sRNA 8.62086 kcal/mol@LB@@LB@Position - mRNA 70 -- 101@LB@Position - sRNA 63 -- 93@LB@Position Seed - mRNA 95 -- 101@LB@Position Seed - sRNA 63 -- 69@LB@
89	0.004143	0.1596	stm2957	ygcA	-15.40	0.101796	159 -- 208	35 -- 82	23S rRNA (uracil(1939)-C(5))-methyltransferase	_____________158____________________________________________209\n_____________\|______________________________________________\|\n5'-AUA...UACUC_____AACG____GU_________UCAG___________UA_____AAUGA...GUC-3'\n______________UGCAA____GCGC__GACGACGCG____AUCAUAACCGU__AAGUC\n______________\|\|\|\|\|____:\|:\|__:\|\|:\|\|:\|:____\|\|\|\|:\|\|\|\|\|\|__\|\|\|:\|\n______________ACGUU____UGUG__UUGUUGUGU____UAGUGUUGGCA__UUCGG\n3'-UUU...GGUUA________________________UG____________________UUUUC...UCA-5'\n_____________\|______________________________________________\|\n_____________83_____________________________________________34\n	250 -- 256	61 -- 67	-36.475 kcal/mol	14.3441 kcal/mol	11.8308 kcal/mol	158 209@LB@ \| \|@LB@5'-AUA...UACUC AACG GU UCAG UA AAUGA...GUC-3'@LB@ UGCAA GCGC GACGACGCG AUCAUAACCGU AAGUC@LB@ \|\|\|\|\| :\|:\| :\|\|:\|\|:\|: \|\|\|\|:\|\|\|\|\|\| \|\|\|:\|@LB@ ACGUU UGUG UUGUUGUGU UAGUGUUGGCA UUCGG@LB@3'-UUU...GGUUA UG UUUUC...UCA-5'@LB@ \| \|@LB@ 83 34@LB@@LB@@LB@Energy -15.40 kcal/mol@LB@Hybridization Energy -36.475 kcal/mol@LB@Unfolding Energy - mRNA 14.3441 kcal/mol@LB@Unfolding Energy - sRNA 11.8308 kcal/mol@LB@@LB@Position - mRNA 159 -- 208@LB@Position - sRNA 35 -- 82@LB@Position Seed - mRNA 250 -- 256@LB@Position Seed - sRNA 61 -- 67@LB@
56	0.002144	0.1363	pslt006	repA	-15.36	0.103288	219 -- 279	36 -- 87	DNA replication	_____________218_____________________280\n_____________\|_______________________\|\n5'-ACU...ACACC_______UGUU____U_______CGACA...CCC-3'\n______________CGAACAC_____CAC__CAUUAC\n______________\|:\|\|\|\|\|_____\|\|\|__\|\|\|:\|\|\n______________GUUUGUG_____GUG__GUAGUG\n3'-UUU...UUAAC_______UUGUU___UU______UUGGC...UCA-5'\n_____________\|_______________________\|\n_____________88______________________35\n	225 -- 231	83 -- 89	-15.7114 kcal/mol	3.78124 kcal/mol	5.66809 kcal/mol	218 280@LB@ \| \|@LB@5'-ACU...ACACC UGUU U CGACA...CCC-3'@LB@ CGAACAC CAC CAUUAC@LB@ \|:\|\|\|\|\| \|\|\| \|\|\|:\|\|@LB@ GUUUGUG GUG GUAGUG@LB@3'-UUU...UUAAC UUGUU UU UUGGC...UCA-5'@LB@ \| \|@LB@ 88 35@LB@@LB@@LB@Energy -15.36 kcal/mol@LB@Hybridization Energy -15.7114 kcal/mol@LB@Unfolding Energy - mRNA 3.78124 kcal/mol@LB@Unfolding Energy - sRNA 5.66809 kcal/mol@LB@@LB@Position - mRNA 219 -- 279@LB@Position - sRNA 36 -- 87@LB@Position Seed - mRNA 225 -- 231@LB@Position Seed - sRNA 83 -- 89@LB@
111	0.005436	0.1728	stm2683	yfjB	-15.36	0.103307	255 -- 299	43 -- 90	NAD(+) kinase	_____________254___________________________________________________________________300\n_____________\|_____________________________________________________________________\|\n5'-CUC...UUCGC__________GAU____UU______UUCAA__________ACUCACAUU_____AUCAAUCU_______AAGGA...AGG-3'\n______________UUAUUAUGGG___CAGA__CAGGGU________GGGAAGU___________GUC________UCGCUAC\n______________\|:\|:\|\|\|\|\|:___\|\|\|\|__\|\|\|\|\|\|________:\|\|\|\|\|\|___________\|\|\|________\|\|\|\|\|\|\|\n______________AGUGAUACCU___GUCU__GUCCCA________UCCUUCA___________CAG________AGCGAUG\n3'-UUU...UAAUU_________________________UUUACAUG_______CUUUUUCCCAU__________________GCACC...UCA-5'\n_____________\|_____________________________________________________________________\|\n_____________91____________________________________________________________________42\n	179 -- 185	115 -- 121	-40.6869 kcal/mol	14.2494 kcal/mol	12.858 kcal/mol	254 300@LB@ \| \|@LB@5'-CUC...UUCGC GAU UU UUCAA ACUCACAUU AUCAAUCU AAGGA...AGG-3'@LB@ UUAUUAUGGG CAGA CAGGGU GGGAAGU GUC UCGCUAC@LB@ \|:\|:\|\|\|\|\|: \|\|\|\| \|\|\|\|\|\| :\|\|\|\|\|\| \|\|\| \|\|\|\|\|\|\|@LB@ AGUGAUACCU GUCU GUCCCA UCCUUCA CAG AGCGAUG@LB@3'-UUU...UAAUU UUUACAUG CUUUUUCCCAU GCACC...UCA-5'@LB@ \| \|@LB@ 91 42@LB@@LB@@LB@Energy -15.36 kcal/mol@LB@Hybridization Energy -40.6869 kcal/mol@LB@Unfolding Energy - mRNA 14.2494 kcal/mol@LB@Unfolding Energy - sRNA 12.858 kcal/mol@LB@@LB@Position - mRNA 255 -- 299@LB@Position - sRNA 43 -- 90@LB@Position Seed - mRNA 179 -- 185@LB@Position Seed - sRNA 115 -- 121@LB@
184	0.01016	0.1966	stm1690	pspA	-15.34	0.103900	116 -- 183	38 -- 113	phage shock protein PspA	_____________115____________________________________________________184\n_____________\|______________________________________________________\|\n5'-GCC...UAUCA_____A____AAGU_G___C__A_______UUAAU__________G________CCGUU...UUC-3'\n______________GUGGU_UGAA____U_GCA_GC_AAUUGUA_____CAC_AUAGCA_GGCAUCGC\n______________\|\|\|\|\|_\|\|\|\|____\|_\|\|\|_:\|_\|\|\|\|\|:\|_____\|\|\|_\|:\|:\|\|_::\|\|\|\|:\|\n______________CACCA_ACUU____A_CGU_UG_UUAACGU_____GUG_UGUUGU_UUGUAGUG\n3'-UUU...GAUGG_____G_________G___C__G_______UU______U______G________UUGGC...UCA-5'\n_____________\|______________________________________________________\|\n_____________114____________________________________________________37\n	162 -- 168	67 -- 73	-39.4413 kcal/mol	11.996 kcal/mol	15.147 kcal/mol	115 184@LB@ \| \|@LB@5'-GCC...UAUCA A AAGU G C A UUAAU G CCGUU...UUC-3'@LB@ GUGGU UGAA U GCA GC AAUUGUA CAC AUAGCA GGCAUCGC@LB@ \|\|\|\|\| \|\|\|\| \| \|\|\| :\| \|\|\|\|\|:\| \|\|\| \|:\|:\|\| ::\|\|\|\|:\|@LB@ CACCA ACUU A CGU UG UUAACGU GUG UGUUGU UUGUAGUG@LB@3'-UUU...GAUGG G G C G UU U G UUGGC...UCA-5'@LB@ \| \|@LB@ 114 37@LB@@LB@@LB@Energy -15.34 kcal/mol@LB@Hybridization Energy -39.4413 kcal/mol@LB@Unfolding Energy - mRNA 11.996 kcal/mol@LB@Unfolding Energy - sRNA 15.147 kcal/mol@LB@@LB@Position - mRNA 116 -- 183@LB@Position - sRNA 38 -- 113@LB@Position Seed - mRNA 162 -- 168@LB@Position Seed - sRNA 67 -- 73@LB@
62	0.003015	0.1596	stm1299	gdhA	-15.31	0.105026	136 -- 171	63 -- 91	glutamate dehydrogenase	_____________135____________________________________172\n_____________\|______________________________________\|\n5'-CGG...AAAAC__________UAUUCUGAUAA_______A_________ACAUU...CCA-3'\n______________UGCAAAUACA___________AACGCAA___UACAACC\n______________\|\|\|\|\|\|:\|\|\|___________\|\|\|:\|\|\|___:\|\|\|\|\|\|\n______________ACGUUUGUGU___________UUGUGUU___GUGUUGG\n3'-UUU...GGUUA__________UG________________GUA_______CAUUC...UCA-5'\n_____________\|______________________________________\|\n_____________92_____________________________________62\n	165 -- 171	63 -- 69	-26.3361 kcal/mol	2.66287 kcal/mol	8.61685 kcal/mol	135 172@LB@ \| \|@LB@5'-CGG...AAAAC UAUUCUGAUAA A ACAUU...CCA-3'@LB@ UGCAAAUACA AACGCAA UACAACC@LB@ \|\|\|\|\|\|:\|\|\| \|\|\|:\|\|\| :\|\|\|\|\|\|@LB@ ACGUUUGUGU UUGUGUU GUGUUGG@LB@3'-UUU...GGUUA UG GUA CAUUC...UCA-5'@LB@ \| \|@LB@ 92 62@LB@@LB@@LB@Energy -15.31 kcal/mol@LB@Hybridization Energy -26.3361 kcal/mol@LB@Unfolding Energy - mRNA 2.66287 kcal/mol@LB@Unfolding Energy - sRNA 8.61685 kcal/mol@LB@@LB@Position - mRNA 136 -- 171@LB@Position - sRNA 63 -- 91@LB@Position Seed - mRNA 165 -- 171@LB@Position Seed - sRNA 63 -- 69@LB@
28	0.0007333	0.08814	stm0376	sbmA	-15.26	0.107034	68 -- 102	55 -- 90	peptide transporter	_____________67______________________________103\n_____________\|_______________________________\|\n5'-GCU...AGAAA_______UA____U______GUAG_______UGCCU...AUU-3'\n______________UGCGAAC__AGCG_CACAAC____GUCACAA\n______________\|\|\|:\|\|\|__\|:\|:_\|\|\|\|\|\|____:\|\|\|\|\|\|\n______________ACGUUUG__UUGU_GUGUUG____UAGUGUU\n3'-UUU...GGUUA_______UG____U_________________GGCAU...UCA-5'\n_____________\|_______________________________\|\n_____________91______________________________54\n	99 -- 105	65 -- 71	-26.4432 kcal/mol	7.11088 kcal/mol	6.59083 kcal/mol	67 103@LB@ \| \|@LB@5'-GCU...AGAAA UA U GUAG UGCCU...AUU-3'@LB@ UGCGAAC AGCG CACAAC GUCACAA@LB@ \|\|\|:\|\|\| \|:\|: \|\|\|\|\|\| :\|\|\|\|\|\|@LB@ ACGUUUG UUGU GUGUUG UAGUGUU@LB@3'-UUU...GGUUA UG U GGCAU...UCA-5'@LB@ \| \|@LB@ 91 54@LB@@LB@@LB@Energy -15.26 kcal/mol@LB@Hybridization Energy -26.4432 kcal/mol@LB@Unfolding Energy - mRNA 7.11088 kcal/mol@LB@Unfolding Energy - sRNA 6.59083 kcal/mol@LB@@LB@Position - mRNA 68 -- 102@LB@Position - sRNA 55 -- 90@LB@Position Seed - mRNA 99 -- 105@LB@Position Seed - sRNA 65 -- 71@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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