Input and runtime details for job 3202356 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3202356 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:02 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:24:35 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:03:47 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:03:55 CET 2018
	Job Completed		@ Sat Feb 17 02:04:28 CET 2018

DIRECT ACCESS: https://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3202356&toolName=CopraRNA ( 30 days expiry )

Description of the job

GcvB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
97	0.004849	0.1728	stm0832	ybiF	-11.58	0.382043	25 -- 72	45 -- 91	threonine/homoserine exporter RhtA	_____________24______________________73\n_____________\|_______________________\|\n5'-GGC...GCGCG_________________U_____CAGAU...CGU-3'\n______________UGC_GGCGCGAUA_CAU__CAUC\n______________\|\|\|_::\|:\|:\|:\|_\|\|:__\|\|\|\|\n______________ACG_UUGUGUUGU_GUG__GUAG\n3'-UUU...GGUUA___U_________U___UU____UGUUG...UCA-5'\n_____________\|_______________________\|\n_____________92______________________44\n	30 -- 36	79 -- 85	-16.3644 kcal/mol	3.58897 kcal/mol	4.34303 kcal/mol	24 73@LB@ \| \|@LB@5'-GGC...GCGCG U CAGAU...CGU-3'@LB@ UGC GGCGCGAUA CAU CAUC@LB@ \|\|\| ::\|:\|:\|:\| \|\|: \|\|\|\|@LB@ ACG UUGUGUUGU GUG GUAG@LB@3'-UUU...GGUUA U U UU UGUUG...UCA-5'@LB@ \| \|@LB@ 92 44@LB@@LB@@LB@Energy -11.58 kcal/mol@LB@Hybridization Energy -16.3644 kcal/mol@LB@Unfolding Energy - mRNA 3.58897 kcal/mol@LB@Unfolding Energy - sRNA 4.34303 kcal/mol@LB@@LB@Position - mRNA 25 -- 72@LB@Position - sRNA 45 -- 91@LB@Position Seed - mRNA 30 -- 36@LB@Position Seed - sRNA 79 -- 85@LB@
98	0.004857	0.1728	stm2811	proX	-15.53	0.097032	195 -- 224	56 -- 84	glycine betaine ABC transporter substrate-binding protein	_____________194________________________225\n_____________\|__________________________\|\n5'-GCA...AAGGA__________________________UAUUU...CUA-3'\n______________ACAACGAUGCGACAU____ACCGUGA\n______________\|\|\|\|\|:\|::\|:\|\|\|\|____\|\|\|\|\|:\|\n______________UGUUGUUGUGUUGUA____UGGCAUU\n3'-UUU...GUUUG_______________GUGU_______CGGUU...UCA-5'\n_____________\|__________________________\|\n_____________85_________________________55\n	210 -- 216	59 -- 65	-26.0076 kcal/mol	3.5169 kcal/mol	9.80321 kcal/mol	194 225@LB@ \| \|@LB@5'-GCA...AAGGA UAUUU...CUA-3'@LB@ ACAACGAUGCGACAU ACCGUGA@LB@ \|\|\|\|\|:\|::\|:\|\|\|\| \|\|\|\|\|:\|@LB@ UGUUGUUGUGUUGUA UGGCAUU@LB@3'-UUU...GUUUG GUGU CGGUU...UCA-5'@LB@ \| \|@LB@ 85 55@LB@@LB@@LB@Energy -15.53 kcal/mol@LB@Hybridization Energy -26.0076 kcal/mol@LB@Unfolding Energy - mRNA 3.5169 kcal/mol@LB@Unfolding Energy - sRNA 9.80321 kcal/mol@LB@@LB@Position - mRNA 195 -- 224@LB@Position - sRNA 56 -- 84@LB@Position Seed - mRNA 210 -- 216@LB@Position Seed - sRNA 59 -- 65@LB@
99	0.004942	0.1728	stm0604	ybdM	-18.77	0.028138	203 -- 249	36 -- 81	similar to E. coli orf hypothetical protein (AAC73702.1); Blastp hit to AAC73702.1 (209 aa) 77% identity in aa 5 - 209	_____________202___________________250\n_____________\|_____________________\|\n5'-AAA...CUUAU______C______________AAAUA...AUA-3'\n______________GCAACA_CGAC_UCACAACCG\n______________:\|\|\|\|\|_\|:\|\|_\|\|\|\|\|\|\|\|\|\n______________UGUUGU_GUUG_AGUGUUGGC\n3'-UUU...UGUGU___________U_________AUUCG...UCA-5'\n_____________\|_____________________\|\n_____________82____________________35\n	216 -- 222	62 -- 68	-25.0091 kcal/mol	1.37283 kcal/mol	7.72388 kcal/mol	202 250@LB@ \| \|@LB@5'-AAA...CUUAU C AAAUA...AUA-3'@LB@ GCAACA CGAC UCACAACCG@LB@ :\|\|\|\|\| \|:\|\| \|\|\|\|\|\|\|\|\|@LB@ UGUUGU GUUG AGUGUUGGC@LB@3'-UUU...UGUGU U AUUCG...UCA-5'@LB@ \| \|@LB@ 82 35@LB@@LB@@LB@Energy -18.77 kcal/mol@LB@Hybridization Energy -25.0091 kcal/mol@LB@Unfolding Energy - mRNA 1.37283 kcal/mol@LB@Unfolding Energy - sRNA 7.72388 kcal/mol@LB@@LB@Position - mRNA 203 -- 249@LB@Position - sRNA 36 -- 81@LB@Position Seed - mRNA 216 -- 222@LB@Position Seed - sRNA 62 -- 68@LB@
100	0.00495	0.1728	stm1879	ptrB	-19.83	0.018631	144 -- 223	40 -- 120	oligopeptidase B	_____________143____________________________________________224\n_____________\|______________________________________________\|\n5'-AAG...UAUAA_________________AAGAAAAA_____A___U___________AAUCG...AGC-3'\n______________CAAAUAU__UAACGCAA________ACGAC_UGU__GCCAAAAGCC\n______________\|\|\|\|:\|:__:\|\|\|:\|\|\|________\|\|:\|\|_:\|\|__\|\|\|\|\|\|\|\|\|\|\n______________GUUUGUG__GUUGUGUU________UGUUG_GCA__CGGUUUUCGG\n3'-UUU...UUAAC_______UU________GUAG_____________UU__________GUAGU...UCA-5'\n_____________\|______________________________________________\|\n_____________121____________________________________________39\n	209 -- 215	49 -- 55	-34.4525 kcal/mol	3.62291 kcal/mol	15.2208 kcal/mol	143 224@LB@ \| \|@LB@5'-AAG...UAUAA AAGAAAAA A U AAUCG...AGC-3'@LB@ CAAAUAU UAACGCAA ACGAC UGU GCCAAAAGCC@LB@ \|\|\|\|:\|: :\|\|\|:\|\|\| \|\|:\|\| :\|\| \|\|\|\|\|\|\|\|\|\|@LB@ GUUUGUG GUUGUGUU UGUUG GCA CGGUUUUCGG@LB@3'-UUU...UUAAC UU GUAG UU GUAGU...UCA-5'@LB@ \| \|@LB@ 121 39@LB@@LB@@LB@Energy -19.83 kcal/mol@LB@Hybridization Energy -34.4525 kcal/mol@LB@Unfolding Energy - mRNA 3.62291 kcal/mol@LB@Unfolding Energy - sRNA 15.2208 kcal/mol@LB@@LB@Position - mRNA 144 -- 223@LB@Position - sRNA 40 -- 120@LB@Position Seed - mRNA 209 -- 215@LB@Position Seed - sRNA 49 -- 55@LB@
101	0.005112	0.1728	stm0113	leuA	-13.72	0.187230	187 -- 230	37 -- 84	2-isopropylmalate synthase	_____________186_____________________231\n_____________\|_______________________\|\n5'-AUA...UGGAG____G_____________G____AGCAA...UGG-3'\n______________ACAA_GAC_C_ACAUCAU_AGCC\n______________\|\|\|\|_:\|\|_\|_\|\|\|\|\|\|:_\|:\|\|\n______________UGUU_UUG_G_UGUAGUG_UUGG\n3'-UUU...GUUUG____G___U_U____________CAUUC...UCA-5'\n_____________\|_______________________\|\n_____________85______________________36\n	196 -- 202	67 -- 73	-19.8594 kcal/mol	2.76514 kcal/mol	8.15907 kcal/mol	186 231@LB@ \| \|@LB@5'-AUA...UGGAG G G AGCAA...UGG-3'@LB@ ACAA GAC C ACAUCAU AGCC@LB@ \|\|\|\| :\|\| \| \|\|\|\|\|\|: \|:\|\|@LB@ UGUU UUG G UGUAGUG UUGG@LB@3'-UUU...GUUUG G U U CAUUC...UCA-5'@LB@ \| \|@LB@ 85 36@LB@@LB@@LB@Energy -13.72 kcal/mol@LB@Hybridization Energy -19.8594 kcal/mol@LB@Unfolding Energy - mRNA 2.76514 kcal/mol@LB@Unfolding Energy - sRNA 8.15907 kcal/mol@LB@@LB@Position - mRNA 187 -- 230@LB@Position - sRNA 37 -- 84@LB@Position Seed - mRNA 196 -- 202@LB@Position Seed - sRNA 67 -- 73@LB@
102	0.005164	0.1728	stm0668	ybeY	-18.23	0.034693	146 -- 193	37 -- 81	rRNA maturation RNase YbeY	_____________145__________________________________________194\n_____________\|____________________________________________\|\n5'-UCC...AAAAC____________UUG____________G_______GU_______AGGAG...CGC-3'\n______________GCAAA_GCGGCG___GCGGCA____GA_CGUAAGC__GAAGCCC\n______________\|\|\|\|\|_:\|::\|:___:\|::\|\|____:\|_\|\|\|\|\|\|\|__:\|\|\|\|\|\|\n______________CGUUU_UGUUGU___UGUUGU____UU_GCAUUCG__UUUCGGG\n3'-UUU...GUUAA_____G______UG_______AGUG__G_______GU_______UAGUC...UCA-5'\n_____________\|____________________________________________\|\n_____________82___________________________________________36\n	178 -- 184	48 -- 54	-38.2699 kcal/mol	15.4401 kcal/mol	16.4278 kcal/mol	145 194@LB@ \| \|@LB@5'-UCC...AAAAC UUG G GU AGGAG...CGC-3'@LB@ GCAAA GCGGCG GCGGCA GA CGUAAGC GAAGCCC@LB@ \|\|\|\|\| :\|::\|: :\|::\|\| :\| \|\|\|\|\|\|\| :\|\|\|\|\|\|@LB@ CGUUU UGUUGU UGUUGU UU GCAUUCG UUUCGGG@LB@3'-UUU...GUUAA G UG AGUG G GU UAGUC...UCA-5'@LB@ \| \|@LB@ 82 36@LB@@LB@@LB@Energy -18.23 kcal/mol@LB@Hybridization Energy -38.2699 kcal/mol@LB@Unfolding Energy - mRNA 15.4401 kcal/mol@LB@Unfolding Energy - sRNA 16.4278 kcal/mol@LB@@LB@Position - mRNA 146 -- 193@LB@Position - sRNA 37 -- 81@LB@Position Seed - mRNA 178 -- 184@LB@Position Seed - sRNA 48 -- 54@LB@
103	0.005167	0.1728	stm2670	aroF	-17.31	0.049328	173 -- 242	25 -- 90	3-deoxy-7-phosphoheptulonate synthase	_____________172______________________________________________________________________243\n_____________\|________________________________________________________________________\|\n5'-CUU...CUAUC_______AGAA__G____G_____C___AU____A_____A_G____U___UAACG_______CA_______ACAGG...AGG-3'\n______________GCAAACA____AA_ACAG_AUCGC_AUC____GC_AAAAG_C_CG_C_GAA_____UACGUAU__CCGAUGA\n______________\|\|\|\|\|\|\|____\|\|_\|\|\|:_\|\|\|:\|_\|:\|____\|\|_\|\|\|\|\|_\|_\|:_\|_\|\|\|_____:\|\|\|:\|\|__\|\|\|\|\|:\|\n______________CGUUUGU____UU_UGUU_UAGUG_UGG____CG_UUUUC_G_GU_G_CUU_____GUGCGUA__GGCUAUU\n3'-UUU...GUUAA_______GUUG__G____G_____U___CAUU__G_______G__A_U_______________A________UUGAA...UCA-5'\n_____________\|________________________________________________________________________\|\n_____________91_______________________________________________________________________24\n	226 -- 232	34 -- 40	-46.7665 kcal/mol	10.372 kcal/mol	24.1795 kcal/mol	172 243@LB@ \| \|@LB@5'-CUU...CUAUC AGAA G G C AU A A G U UAACG CA ACAGG...AGG-3'@LB@ GCAAACA AA ACAG AUCGC AUC GC AAAAG C CG C GAA UACGUAU CCGAUGA@LB@ \|\|\|\|\|\|\| \|\| \|\|\|: \|\|\|:\| \|:\| \|\| \|\|\|\|\| \| \|: \| \|\|\| :\|\|\|:\|\| \|\|\|\|\|:\|@LB@ CGUUUGU UU UGUU UAGUG UGG CG UUUUC G GU G CUU GUGCGUA GGCUAUU@LB@3'-UUU...GUUAA GUUG G G U CAUU G G A U A UUGAA...UCA-5'@LB@ \| \|@LB@ 91 24@LB@@LB@@LB@Energy -17.31 kcal/mol@LB@Hybridization Energy -46.7665 kcal/mol@LB@Unfolding Energy - mRNA 10.372 kcal/mol@LB@Unfolding Energy - sRNA 24.1795 kcal/mol@LB@@LB@Position - mRNA 173 -- 242@LB@Position - sRNA 25 -- 90@LB@Position Seed - mRNA 226 -- 232@LB@Position Seed - sRNA 34 -- 40@LB@
104	0.005232	0.1728	stm0744	ybgC	-16.85	0.058772	132 -- 175	54 -- 90	tol-pal system-associated acyl-CoA thioesterase	_____________131_____________________________176\n_____________\|_______________________________\|\n5'-CUU...ACAAG____U____________UCUUGA________GGCGG...AAA-3'\n______________GCGA_AUACAACGGCAU______GGCAUUAC\n______________\|\|:\|_\|:\|\|\|\|\|::\|\|:______::\|\|\|:\|\|\n______________CGUU_UGUGUUGUUGUG______UUGUAGUG\n3'-UUU...GUUAA_______________________________UUGGC...UCA-5'\n_____________\|_______________________________\|\n_____________91______________________________53\n	156 -- 162	67 -- 73	-23.8418 kcal/mol	6.2547 kcal/mol	5.68203 kcal/mol	131 176@LB@ \| \|@LB@5'-CUU...ACAAG U UCUUGA GGCGG...AAA-3'@LB@ GCGA AUACAACGGCAU GGCAUUAC@LB@ \|\|:\| \|:\|\|\|\|\|::\|\|: ::\|\|\|:\|\|@LB@ CGUU UGUGUUGUUGUG UUGUAGUG@LB@3'-UUU...GUUAA UUGGC...UCA-5'@LB@ \| \|@LB@ 91 53@LB@@LB@@LB@Energy -16.85 kcal/mol@LB@Hybridization Energy -23.8418 kcal/mol@LB@Unfolding Energy - mRNA 6.2547 kcal/mol@LB@Unfolding Energy - sRNA 5.68203 kcal/mol@LB@@LB@Position - mRNA 132 -- 175@LB@Position - sRNA 54 -- 90@LB@Position Seed - mRNA 156 -- 162@LB@Position Seed - sRNA 67 -- 73@LB@
105	0.005236	0.1728	stm4566	yjjI	-12.28	0.305832	96 -- 137	40 -- 77	YjjI family glycine radical enzyme	_____________95________________138\n_____________\|_________________\|\n5'-ACA...UUUUC____________U____CCGGC...UGG-3'\n______________UACAAUAGCGCA_CGUC\n______________:\|\|\|\|:\|:\|:\|\|_\|:\|\|\n______________GUGUUGUUGUGU_GUAG\n3'-UUU...CGUUU____________U____UGUUG...UCA-5'\n_____________\|_________________\|\n_____________78________________39\n	167 -- 173	74 -- 80	-18.3997 kcal/mol	3.44142 kcal/mol	3.92987 kcal/mol	95 138@LB@ \| \|@LB@5'-ACA...UUUUC U CCGGC...UGG-3'@LB@ UACAAUAGCGCA CGUC@LB@ :\|\|\|\|:\|:\|:\|\| \|:\|\|@LB@ GUGUUGUUGUGU GUAG@LB@3'-UUU...CGUUU U UGUUG...UCA-5'@LB@ \| \|@LB@ 78 39@LB@@LB@@LB@Energy -12.28 kcal/mol@LB@Hybridization Energy -18.3997 kcal/mol@LB@Unfolding Energy - mRNA 3.44142 kcal/mol@LB@Unfolding Energy - sRNA 3.92987 kcal/mol@LB@@LB@Position - mRNA 96 -- 137@LB@Position - sRNA 40 -- 77@LB@Position Seed - mRNA 167 -- 173@LB@Position Seed - sRNA 74 -- 80@LB@
106	0.005253	0.1728	stm1172	flgM	-15.84	0.086212	196 -- 254	36 -- 82	anti-sigma-28 factor FlgM	_____________195________________255\n_____________\|__________________\|\n5'-GGC...GACGC__G_______________CCGGU...CGU-3'\n______________GC_AAAC_CAGCGACACG\n______________\|\|_\|\|\|\|_\|\|:\|:\|\|\|\|:\n______________CG_UUUG_GUUGUUGUGU\n3'-UUU...GUUAA_______U__________UGUAG...UCA-5'\n_____________\|__________________\|\n_____________83_________________35\n	263 -- 269	74 -- 80	-18.0556 kcal/mol	5.48392 kcal/mol	3.73436 kcal/mol	195 255@LB@ \| \|@LB@5'-GGC...GACGC G CCGGU...CGU-3'@LB@ GC AAAC CAGCGACACG@LB@ \|\| \|\|\|\| \|\|:\|:\|\|\|\|:@LB@ CG UUUG GUUGUUGUGU@LB@3'-UUU...GUUAA U UGUAG...UCA-5'@LB@ \| \|@LB@ 83 35@LB@@LB@@LB@Energy -15.84 kcal/mol@LB@Hybridization Energy -18.0556 kcal/mol@LB@Unfolding Energy - mRNA 5.48392 kcal/mol@LB@Unfolding Energy - sRNA 3.73436 kcal/mol@LB@@LB@Position - mRNA 196 -- 254@LB@Position - sRNA 36 -- 82@LB@Position Seed - mRNA 263 -- 269@LB@Position Seed - sRNA 74 -- 80@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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