Input and runtime details for job 4627738 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_003210
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 4627738 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:20:43 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:22:37 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:24:00 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:24:55 CET 2020
	Job Completed		@ Mon Jan 27 14:25:04 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=4627738&toolName=IntaRNA ( 30 days expiry )

Description of the job

LhrA2 - Listeria monocytogenes

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_003210 Listeria monocytogenes EGD-e

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
lmo0129	-14	15	-14	-8	2.87	LhrA2	176	203	197	203	6.34	-16.42	-25.63	-1.54828	GGAUGAGGAAAAACAUGACAAAGAUAUGG&CCGUGUAGUUCAUGUCUAUGCCUCAUCC	((((((((....((((((.....((((((&))))))...)))))).....))))))))	GAAUGAAGGUCCCGGAAGUAUUGAAAAAUUUAAUUUUGAUUUUCGAUGCAAAGUCAGGAGAGGAUGAGGAAAAACAUGACAAAGAUAUGGAUUGACGCUGGACACGGUGGUAAGGAUUCAGGUGCGAGUGGUAAUGGACUUGUUGAAAAA	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.0070414	0.863511		986713	N-acetylmuramoyl-L-alanine amidase
lmo0200	-10	14	-10	-4	4.26	LhrA2	175	204	198	204	6.62	-14.21	-25.09	-1.33989	GGGAUGAGACAUGAACGCUCAAGC&GCCGUGUAGUUCAUGUCUAUGCCUCAUCCC	((((((((((((((((......((&))......)))))))))......)))))))	AACAAUUGUUGUUACUGCCUAAUGUUUUUAGGGUAUUUUAAAAAAGGGCGAUAAAAAACGAUUGGGGGAUGAGACAUGAACGCUCAAGCAGAAGAAUUCAAAAAAUAUUUAGAAACUAACGGGAUAAAACCAAAACAAUUUCAUAAAAAA	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.0231743	0.9474659	prfA	987031	listeriolysin positive regulatory protein
lmo0788	-23	-4	-11	-5	4.93	LhrA2	197	212	198	204	3.03	-14.54	-22.5	-1.37101	UGCGUAACGAAAGGGAUGAG&CUCAUCCCGUUAUGCA	((((((((....((((((((&))))))))))))))))	AAGCGAAGUACAAUUAACGGAUGAUAUGUGAAAUCAUAUACAUAUUAAGUAGUGCGUAACGAAAGGGAUGAGAAAAUGAUUCAUGCAGGAUUAGAUGUAGGAUCAACAACAGCAAAGGCCGUAGCACUUAAUGACCAGGGGGAUAUUUUA	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.0195222	0.9163749		985448	lmo0788
lmo0649	-9	5	-9	-3	2.94	LhrA2	190	204	198	204	4.18	-14.7	-21.82	-1.38609	GGGAUGAGCAUGGA&UCUAUGCCUCAUCCC	((((((((((((((&)))))).))))))))	AUUAAUUACAACGGGAGUAACCGUGAUUUUUUGGCGUAGAAAAUACUUUUAAUAUAUAGAAAACGAGGGAUGAGCAUGGAAAAACAAACAUACGAAAAAUUAGCAUAUUAUACAAUCAAAGAAAAAAUUCUCAGUGGAAAGCUUCAUGUA	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.0179502	0.9128702		986557	transcriptional regulator
lmo0137	-20	28	-17	-11	8.9	LhrA2	154	207	198	204	7.28	-13.49	-29.67	-1.272	AAUGGGGUGAGGAAUUCAUGAUGCGAUUAGAUUUUUCGAAAAAAACAA&UUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUU	((((((((((((....(((((..((((.((((.....(((.(((((((&)))))))..))))))).))))..........)))))......))))))))))))	UCGGAAACUUGCUGGCCGAUUUAGCUUAUAUGAUUGUCGAUCCGCGGAUUAGGGAAAUGGGGUGAGGAAUUCAUGAUGCGAUUAGAUUUUUCGAAAAAAACAAUGCAAGAUUUCGAGCGGGAUGCAGAAGUCGUUCACGCAACACGAGAG	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.0334303	0.9493467		986757	peptide ABC transporter permease
lmo2106	-29	22	-29	-23	8.45	LhrA2	159	207	201	207	7.26	-14.28	-29.99	-1.34649	AAUGGGAACAGCAACAUAGAGGUGAAAAAAUGUCUUUAAAGAUGAAGUGGA&UUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUU	(((((((..((...((((((..((((.....(((.....(((((((.((((&)))))))))))....))).......))))..)))))).))..)))))))	AUAAUGAUAGGGAUAACGUUUAAAGGAUGACAAAAUAUUGAUAAUAAAUGGGAACAGCAACAUAGAGGUGAAAAAAUGUCUUUAAAGAUGAAGUGGAUUAUAGCGAUCAGUAUUAUCAUUGUACUUUUAGUCGUGGGGUGGUUUCUUGGG	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.0223506	0.9474659		984749
lmo1941	23	69	23	29	9.14	LhrA2	156	207	201	207	7.27	-15.29	-31.7	-1.44173	AACGGGAAUACGAAGAACCGGCUGAUUAUGACAUGAGUUCUGAAGGC&GUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUU	((((((((((.(((..((((((((((...((.((((((...((((((&)))))))))))).)))))))))).))..))).)))..........)))))))	AUCAUAUGUAACACUUCUCUGUGCCAAUAAUAGUGCAUAGGAUAAGUUUAGAAAGCACUAAAGGAGGAACUUUGAGUGGUAAAAACAAGAAAAGAAAAACGGGAAUACGAAGAACCGGCUGAUUAUGACAUGAGUUCUGAAGGCCAAGAU	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.0131118	0.8878302		987990
lmo1835	-25	28	-25	-19	8.84	LhrA2	154	207	201	207	7.28	-18.61	-34.73	-1.75478	AAUGGGAAAGAGGAGGGUGAACUACAUGCCUAAACGCGACGAUAUAAAAACAA&UUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUU	(((((((..((((((..(((((((((((.((((....((.((....(((((((&)))))))...)).))...)))).)))))))))))...))...)))).)))))))	CAGGGCCAAGUGACGUCAACUACUUAUUUGAUGAAUUUAUGGAAAUGAUGAAUGGGAAAGAGGAGGGUGAACUACAUGCCUAAACGCGACGAUAUAAAAACAAUUCUAGUAAUCGGUUCUGGCCCAAUUGUUAUUGGACAGGCGGCAGAG	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.0019491	0.863511	carB	985876	carbamoyl-phosphate synthetase
lmo0022	-2	42	-2	5	5.81	LhrA2	160	208	202	208	7.37	-14.13	-27.31	-1.33235	UGAUGGGAAGCAACGGAAUUAAACACGUACGCGUAGAUGAAAGA&UCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUA	((((((((.(((((.(((((..(((((...((..(((((((.((&)).))))))).....))))))))))))..))...)))....))))))))	AAAAGAUAAUAUUCAAUUUGUAGAGCAAAAAGCGAGUCAUACGAUAGUAAGUGAUGAUGGGGAGGAAGAGGACUGAUGGGAAGCAACGGAAUUAAACACGUACGCGUAGAUGAAAGACUAAUUCACGGACAAGUAGCAACAAUGUGGACC	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.02415	0.9474659		985051	PTS fructose transporter subunit IIB
lmo1828	11	21	14	20	0.66	LhrA2	201	211	202	208	2.18	-16.11	-18.95	-1.51905	GCAUGACGGGA&UCCCGUUAUGC	(((((((((((&)))))))))))	UGGUAUAAUAAGAAGUCAGAACAAAUUUCAAGUAAUCUUAGAGGGAACUUUAAGUUAUGAAAGGUGUCUUGCCAAAUGGUGAAAAGCAUGACGGGAUUUGGGCGUGCCACAAAAGAAUUUGAAGCGUUCAAAGUAACCAUUGAAUUAAAA	AGGAUAGAGUUUUACCUCGAUGAAAGUUUCAUUUUUACUAAUGUGAUUUUCAUGGGGAUAAGACCCUAUCAUGGUUAUAAUGACUUUUGGUGUAUCCAUCUUCCAAUAGAAUGGCAACUAGUCAGACAUCUGUUUAGACACCAAAAUCAUUAUUUGUUUUCAUUCAUCUCAUUGGCCGUGUAGUUCAUGUCUAUGCCUCAUCCCGUUAUGCAUAGGCGCCCGAACAUGAUGGCAACGCUGGAAAUGUUUAUUUCGAGCGUCUUUUUUUG	0.0083732	0.863511		985886

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching