Input and runtime details for job 8403881 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_000913
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 8403881 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:15:25 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:18:18 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:19:13 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:20:45 CET 2020
	Job Completed		@ Mon Jan 27 14:20:57 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=8403881&toolName=IntaRNA ( 30 days expiry )

Description of the job

RyhB - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
b2830	62	74	68	74	1.12	RyhB	44	56	44	50	3.2	-13.5	-17.82	-1.41949	AGGUAAUGUGGGC&GCUCACAUUGCUU	(((((((((((((&)))))))))))))	GCAGAGAGUGGUUUACCGUCUUCCUGGAGUAUGAAACAAUCAUUCGUAUAUAAAGCUUUAUUUUGAGGUAGUCCGGUGAUUGAUGACGAUGGCUACCGCCCAAACGUAGGUAUCGUGAUUUGUAAUCGCCAGGGGCAGGUAAUGUGGGCC	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0072388	0.9291058	rppH	947300	RNA pyrophosphohydrolase
b3010	-68	-29	-35	-29	12.31	RyhB	38	86	38	44	14.91	-11.97	-39.19	-1.25862	GCCAGCGCCCUGGCUCUCAAUGCUCAUUUCUGCCAAUGUC&GACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGC	(((((((((((((((...((((((((.......(((((((&))))))).......)).....))))))....)))))...))))))))))	UGUGAUCGCCAGCGCCCUGGCUCUCAAUGCUCAUUUCUGCCAAUGUCUUGCCUAUUUCUCCAGAGUGCUGGAGAAAUGCUACAAAAUUGCGCACAAUCAAAUUGCCGCAUUAUUCCUAAGAAAUUACGCGAUAUGAAACGUGAAGAGAUU	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0167745	0.9496255	yqhC	947491	transcriptional activator of yqhD
b3032	-67	-43	-49	-43	8.96	RyhB	40	63	40	46	3.3	-10.86	-23.12	-1.1419	AUACUGUUUAGCACAUGGAGCGAUG&CAUUGCUCACAUUGCUUCCAGUAU	((((((...((((.(((((((((((&)))))))).)))))))..))))))	UGGUUGCGAUACUGUUUAGCACAUGGAGCGAUGGCGAUUCCGGUUUAUUAGCGUCGUGAAACCUAAGGACACCAUUUGGAAAGCCUGUUAACCCUUCCUCUGGCUGGUGAGGCCAGAGUCAGGAUUUUACAAAUUACCGACACUCACCUG	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0301134	0.9496255	cpdA	947515	3'5' cAMP phosphodiesterase
b3080	-59	-34	-44	-38	3.58	RyhB	1	25	6	12	7.71	-14.07	-25.36	-1.47943	UCCCAGCGACCAAAUCUUCCUGAUGC&GCGAUCAGGAAGACCCUCGCGGAGA	((((.((((.....((((((((((((&)).))))))))))...)))))).))	AAAAUGCCUCUCACGAUCCCAGCGACCAAAUCUUCCUGAUGCAAUAUUUCUACUGGUAAUAAGGACUGUAAUUUCAUGAAAAUAAAAACUAUUUUAACGCCAGUAACCUGCGCUCUGCUGAUAAGUUUUUCCGCCCAUGCCGCUAACGCC	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0052402	0.9291058	ygjK	947596	alpha-glucosidase
b3084	-35	-25	-32	-26	2.76	RyhB	37	47	38	44	1.15	-13.75	-17.66	-1.44578	GGGCAAUGUCG&CGACAUUGCUC	(((((((((((&)))))))))))	CGAAAAAGAGAAAAAGGGUAUACUCACGCACCCUUUUCAGGGGCAAUGUCGAAAUUUUAUCAUUCAGGAGGGCCGAUGAGCCACUUAGACAACGGUUUCCGUUCACUGACACUACAACGUUUUCCGGCGACGGAUGACGUUAACCCGCUA	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0062866	0.9291058	rlmG	947589	23S rRNA m(2)G1835 methyltransferase SAM-dependent
b3110	57	75	57	63	2.99	RyhB	44	62	56	62	3.3	-13.55	-19.84	-1.42475	UGCUGGAAUGAGUGAGAGC&GCUCACAUUGCUUCCAGUA	((((((((..((((.((((&)))).))))..))))))))	AAACGUAGACCGCAUAUCGCCUUGAUGCGGCUCGGAAAAAAAUAAUAAGCCCUACAAAAUAUUGAGCGAGAAAUUAUGGAAAUUGCAUCGAAUAAAGGCGUCAUUGCAGACGCUUCGACCCCGGCGGGUCGUGCUGGAAUGAGUGAGAGC	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0070381	0.9291058	yhaO	947628	putative transporter
b3219	-10	11	-9	-3	4.85	RyhB	39	59	52	58	3.27	-12.48	-20.6	-1.31224	UGGAGGCAAAAUGAACAAUGU&ACAUUGCUCACAUUGCUUCCA	(((((((((..((..((((((&))))))..))..)))))))))	AGCUUUUAAGGAUGAAGGGGAACUAACCGACAUGACAGAUAUUACAGGCAAUCUGACGGUCCUGGUGGAGGCAAAAUGAACAAUGUAAAAUUACUGAUUGCCGGAAGUGCCUUUUUUGCCAUGUCAGCGCAAGCCGCUGAUAGAGUAUCA	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0127312	0.9496255	yhcF	947735	putative transcriptional regulator
b3248	19	35	29	35	5.01	RyhB	18	35	18	24	9.68	-12.53	-27.22	-1.3175	GCUUCCGGUUCUCCGCG&CGCGGAGAACCUGAAAGC	(((((.(((((((((((&))))))))))).)).)))	UGGGGUGCUCUGGCCGUGGAUUUUCUUGCUGAUGCGCAAAGUCCGUCAGCAGUUUGCAGUGCAAUAAAGGUUUCUAUGACUUCUCUGUAUUUAGCUUCCGGUUCUCCGCGUCGUCAGGAGUUACUUGCGCAACUUGGCGUGACCUUUGAA	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0123886	0.9496255	yhdE	947753	dTTP/UTP pyrophosphatase m(5)UTP/m(5)CTP/pseudo-UTP pyrophosphatase
b3311	-68	-41	-49	-43	11.36	RyhB	37	60	39	45	3.3	-11.34	-26	-1.19237	CUGUUGAAGCAAGUGCGUCGCGAUGUCG&CGACAUUGCUCACAUUGCUUCCAG	(((..((((((((((....(((((((((&))))))))).))).))))))))))	GUCUCACCUGUUGAAGCAAGUGCGUCGCGAUGUCGCACGCGUUAAGACUUUACUGAACGAGAAGGCGGGUGCGUAAUGACCGAUAAAAUCCGUACUCUGCAAGGUCGCGUUGUUAGCGACAAAAUGGAGAAAUCCAUUGUUGUUGCUAUC	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0234421	0.9496255	rpsQ	947808	30S ribosomal subunit protein S17
b3365	-9	12	2	8	4.28	RyhB	38	57	42	48	3.21	-10.98	-18.47	-1.15452	GAGGCAAAAAUGAGCAAAGUC&GACAUUGCUCACAUUGCUUC	(((((((...(((((((..((&))..)))))))..)))))))	AAUUUGAUUUACAUCAAUAAGCGGGGUUGCUGAAUCGUUAAGGUAGGCGGUAAUAGAAAAGAAAUCGAGGCAAAAAUGAGCAAAGUCAGACUCGCAAUUAUCGGUAACGGUAUGGUCGGCCAUCGCUUUAUCGAAGAUCUUCUUGAUAAA	GCGAUCAGGAAGACCCUCGCGGAGAACCUGAAAGCACGACAUUGCUCACAUUGCUUCCAGUAUUACUUAGCCAGCCGGGUGCUGGCUUUU	0.0282964	0.9496255	nirB	947868	nitrite reductase large subunit NAD(P)H-binding

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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