Input and runtime details for job 6244847 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_000913
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 6244847 (server version trunk)

	Job Submitted & Queued		@ Fri Jan 07 21:36:19 CET 2022
	Pre-Processing Started		@ Fri Jan 07 21:36:23 CET 2022
	IntaRNA Started		@ Fri Jan 07 21:38:06 CET 2022
	IntaRNA Finished & Post-Processing		@ Fri Jan 07 21:38:48 CET 2022
	Post-Processing Finished		@ Fri Jan 07 21:39:11 CET 2022
	Job Completed		@ Fri Jan 07 21:39:12 CET 2022

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6244847&toolName=IntaRNA ( 30 days expiry )

Description of the job

ChiX - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
b3141	41	51	41	47	3.69	ChiX	38	48	42	48	1.04	-8.7	-13.43	-0.921468	AGGAAUUUCAU&AUGAAAUUCCU	(((((((((((&)))))))))))	AAAGCGCAUCCGGUGCUGUUAAUCGGCGUGACUUUUGUGCUCUCUAUUGUUUGUUCCGCAUUCGGCAUUUUGUAAAUGGAACGAGGCACUGCGUCUGGUGGUGCCUCUUUACUUAAGGAAUUUCAUCCUGUGCAAACCCUUCAGCAAGUU	ACACCGUCGCUUAAAGUGACGGCAUAAUAAUAAAAAAAUGAAAUUCCUCUUUGACGGGCCAAUAGCGAUAUUGGCCAUUUUUUU	0.0333715	0.8602904	agaI	947985	putative deaminase AgaI
b1363	-74	-59	-74	-68	0.44	ChiX	36	51	45	51	1.07	-11.89	-13.4	-1.25934	AAGAGGAGUUAAAUUU&AAAUGAAAUUCCUCUU	((((((((((..((((&))))..))))))))))	UAAGAGGAGUUAAAUUUUAUACAGUAUAAUCAUAAUAUUGCAGCAAGGUGGUUAUAAUUGAAAGAAUAUUUAGAUAUGAAUACAUCUCAUGUAAGAGUUGUUACUCAUAUGUGUGGGUUCCUGGUUUGGCUCUAUAGUCUUUCAAUGUUG	ACACCGUCGCUUAAAGUGACGGCAUAAUAAUAAAAAAAUGAAAUUCCUCUUUGACGGGCCAAUAGCGAUAUUGGCCAUUUUUUU	0.0071174	0.8602904	trkG	945932	Rac prophage K(+) transporter TrkG
b2851	-15	-4	-10	-4	2.08	ChiX	43	54	43	49	0.94	-10.38	-13.4	-1.09941	UCACAGAGGAAU&AUUCCUCUUUGA	(((.((((((((&)))))))).)))	AAAGAUAAAUAUAAAAAUAUCAAUGAGUCAAUCAAUAAUAAUUUGGCAUCACGAGACACAUCACAGAGGAAUAUUAUGAGCACAGAAACAAUUGAAAUAUUCAAUAAUAGUGAUGAAUGGGCAAAUCAACUAAAACACGCAUUAUCGAAA	ACACCGUCGCUUAAAGUGACGGCAUAAUAAUAAAAAAAUGAAAUUCCUCUUUGACGGGCCAAUAGCGAUAUUGGCCAUUUUUUU	0.0146892	0.8602904	ygeG	946986	TPR repeat-containing putative chaperone YgeG
b1071	-17	-6	-12	-6	4.12	ChiX	43	54	43	49	0.94	-8.34	-13.4	-0.883339	UCAAUGAGGAAU&AUUCCUCUUUGA	((((.(((((((&))))))).))))	GCUGUUAAAGAUUACCCGUCCCUUGCCGAUAAAUAAGCAACACAUGAUAAAAGCGCCCUCAAUGAGGAAUAAACCAUGAGUAUUGAUCGCACUUCGCCUCUGAAGCCUGUAAGCACCGUUCAACCGCGCGAAACCACUGACGCGCCGGUA	ACACCGUCGCUUAAAGUGACGGCAUAAUAAUAAAAAAAUGAAAUUCCUCUUUGACGGGCCAAUAGCGAUAUUGGCCAUUUUUUU	0.0398471	0.8602904	flgM	946684	anti-sigma factor for FliA (sigma(28))
b0228	26	36	27	33	4.14	ChiX	44	54	47	53	0.93	-8.24	-13.31	-0.872747	UCAAGGGGGGA&UUCCUCUUUGA	(((((((((((&)))))))))))	GCCGCUUUCCCGAUAUAAAACAACCUCAUUGCCAACCUUUCCUUUUCUUCUUACCGUUGAGAAAAAGGAGUCGCCAUGUCUGAAUAUCGUCGUUAUUACAUCAAGGGGGGAACAUGGUUUUUCACGGUGAAUUUACGAAAUCGUCGAAGC	ACACCGUCGCUUAAAGUGACGGCAUAAUAAUAAAAAAAUGAAAUUCCUCUUUGACGGGCCAAUAGCGAUAUUGGCCAUUUUUUU	0.041862	0.8602904	rayT	944913	REP-associated tyrosine transposase
b4175	-16	-5	-11	-5	1.9	ChiX	43	55	43	49	0.94	-10.44	-13.28	-1.10576	GUCAGGGGGAAU&AUUCCUCUUUGAC	((((((((((((&))))))))).)))	CAGUCAGGGCGAUAUUAUGGACCAACGCCGCGCCAACGCGCAGCGUAACGACUACCAGCGUCAGGGGGAAUAACGAUGCGUAAGUCAGUUAUCGCGAUUAUCAUCAUCGUGCUGGUAGUGCUUUACAUGUCUGUCUUUGUCGUCAAAGAA	ACACCGUCGCUUAAAGUGACGGCAUAAUAAUAAAAAAAUGAAAUUCCUCUUUGACGGGCCAAUAGCGAUAUUGGCCAUUUUUUU	0.0142687	0.8602904	hflC	948697	regulator of FtsH protease
b4357	-13	-4	-11	-5	0.42	ChiX	44	53	45	51	0.92	-11.89	-13.23	-1.25934	CAAGGAGGAA&UUCCUCUUUG	((((((((((&))))))))))	GAAAAUCUUUUUUGUGAAAACACACGCAUAAAAAUGUUUUAAAUCGGGUUUUAAUGGACUCACAAGGAGGAAAAUAUGAGUCGUUCACAAAAUUUACGCCACAAUGUGAUUAACCAGGUCAUUGAUGAUAUGGCGCGGGGUCAUAUCCCU	ACACCGUCGCUUAAAGUGACGGCAUAAUAAUAAAAAAAUGAAAUUCCUCUUUGACGGGCCAAUAGCGAUAUUGGCCAUUUUUUU	0.0071174	0.8602904	lgoR	948881	putative DNA-binding transcriptional regulator LgoR
b0542	9	27	10	16	3.59	ChiX	38	53	46	52	1.15	-8.3	-13.04	-0.879102	CAAAGAGGCAAUUAUUCAU&AUGAAAUUCCUCUUUG	((((((((.((((..((((&))))))))))))))))	ACCUCUAAAUCGUGCACAGGCUCUGGCGAAGAUCGCAGAAAUUAAAGCUAAGUUCGGACUGAAAGGAGCAAGUGUAUGACGGGCAAAGAGGCAAUUAUUCAUUACCUGGGGACGCAUAAGAGCUUCUGUGCACAGGACGUUGCCGCGGUA	ACACCGUCGCUUAAAGUGACGGCAUAAUAAUAAAAAAAUGAAAUUCCUCUUUGACGGGCCAAUAGCGAUAUUGGCCAUUUUUUU	0.040641	0.8602904	renD	945024	DLP12 prophage protein RenD
b1048	-42	-33	-41	-35	0.13	ChiX	44	53	46	52	0.92	-11.91	-12.96	-1.26146	CAAAGGGGGA&UUCCUCUUUG	((((((((((&))))))))))	UCCAUUAAAAUAGAUCGGAUCGAUAUAAGCACACAAAGGGGGAAGUGCUUACUAAUUAUGAAACAUAAACUACAAAUGAUGAAAAUGCGUUGGUUGAGUGCUGCAGUAAUGUUAACCCUGUAUACAUCUUCAAGCUGGGCUUUCAGUAUU	ACACCGUCGCUUAAAGUGACGGCAUAAUAAUAAAAAAAUGAAAUUCCUCUUUGACGGGCCAAUAGCGAUAUUGGCCAUUUUUUU	0.0070501	0.8602904	opgG	945005	osmoregulated periplasmic glucans (OPGs) biosynthesis protein G
b3909	-51	-40	-47	-41	2.65	ChiX	42	53	43	49	0.93	-9.37	-12.95	-0.992432	CAUUGAGGAAUU&AAUUCCUCUUUG	((..((((((((&))))))))..))	UCGUUUCCAUCAACACUGAUGCUCCAUUGAGGAAUUACGCAUCAGCCCUUAAAAAUAUGCCGACAGGUGAUGGAAAUGCAGAUAAAACGCUCGAUUGAGAAAAUCCCGGGGGGGAUGAUGCUCGUCCCGCUAUUCCUUGGCGCACUGUGC	ACACCGUCGCUUAAAGUGACGGCAUAAUAAUAAAAAAAUGAAAUUCCUCUUUGACGGGCCAAUAGCGAUAUUGGCCAUUUUUUU	0.0240197	0.8602904	kdgT	948407	2-dehydro-3-deoxy-D-gluconate:H(+) symporter

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.2.0 linking Vienna RNA package 2.4.14

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