| 73 |
0.002371 |
0.1242 |
bsu00930 |
cysE |
-20.00 |
0.002833 |
192 -- 291 |
102 -- 187 |
serine acetyltransferase |
|
_____________191__________________________________________________________________________292\n_____________|____________________________________________________________________________|\n5'-CCU...AAUGC____AGAA__________C___GUUCGAUCAAGAUC__________AAG_____UU__AA__G_____AA______CCGGU...UAC-3'\n______________UCAA____GACAUUGAUA_UGU______________CCGCAGCAAG___C_UAU__UG__GU_AUUUU__CUUAUU\n______________||||____||||||||:|_|||______________||||||||||___|_|||__||__||_|:|||__||:||:\n______________AGUU____CUGUAACUGU_ACA______________GGCGUCGUUC___G_AUA__AC__CA_UGAAA__GAGUAG\n3'-AUU...GAAGU____GUC___________A___________________________AAG_U___UU__AA__G_____________AAGUU...CAA-5'\n_____________|____________________________________________________________________________|\n_____________188__________________________________________________________________________101\n |
256 -- 262 |
130 -- 136 |
-42.0244 kcal/mol |
15.3231 kcal/mol |
14.7636 kcal/mol |
191 292@LB@ | |@LB@5'-CCU...AAUGC AGAA C GUUCGAUCAAGAUC AAG UU AA G AA CCGGU...UAC-3'@LB@ UCAA GACAUUGAUA UGU CCGCAGCAAG C UAU UG GU AUUUU CUUAUU@LB@ |||| ||||||||:| ||| |||||||||| | ||| || || |:||| ||:||:@LB@ AGUU CUGUAACUGU ACA GGCGUCGUUC G AUA AC CA UGAAA GAGUAG@LB@3'-AUU...GAAGU GUC A AAG U UU AA G AAGUU...CAA-5'@LB@ | |@LB@ 188 101@LB@@LB@@LB@Energy -20.00 kcal/mol@LB@Hybridization Energy -42.0244 kcal/mol@LB@Unfolding Energy - mRNA 15.3231 kcal/mol@LB@Unfolding Energy - sRNA 14.7636 kcal/mol@LB@@LB@Position - mRNA 192 -- 291@LB@Position - sRNA 102 -- 187@LB@Position Seed - mRNA 256 -- 262@LB@Position Seed - sRNA 130 -- 136@LB@ |
| 41 |
0.00131 |
0.1206 |
bsu01580 |
ybaR |
-15.69 |
0.023818 |
44 -- 97 |
34 -- 84 |
sulfate transporter YbaR |
|
_____________43________________________________________________________________98\n_____________|_________________________________________________________________|\n5'-CAG...UUAUU____UACU______A__A___CUC____CCU___U_____A_______A_GU_____________UCUCC...CGA-3'\n______________AUGA____CUUUGU_UG_CAA___CUUG___CAA_ACAAU__UACUCA_C_____UUCCCCGUUU\n______________||||____|||:||_||_|||___||||___|||_|||||__|||:||_|_____||||||:|||\n______________UACU____GAAGCA_AC_GUU___GAAC___GUU_UGUUA__AUGGGU_G_____AAGGGGUAAA\n3'-AUU...UUCAU____UACC_____________A______C___________GC______A_AGAAU__________GAAAC...CAA-5'\n_____________|_________________________________________________________________|\n_____________85________________________________________________________________33\n |
90 -- 96 |
35 -- 41 |
-34.7178 kcal/mol |
7.59764 kcal/mol |
14.4179 kcal/mol |
43 98@LB@ | |@LB@5'-CAG...UUAUU UACU A A CUC CCU U A A GU UCUCC...CGA-3'@LB@ AUGA CUUUGU UG CAA CUUG CAA ACAAU UACUCA C UUCCCCGUUU@LB@ |||| |||:|| || ||| |||| ||| ||||| |||:|| | ||||||:|||@LB@ UACU GAAGCA AC GUU GAAC GUU UGUUA AUGGGU G AAGGGGUAAA@LB@3'-AUU...UUCAU UACC A C GC A AGAAU GAAAC...CAA-5'@LB@ | |@LB@ 85 33@LB@@LB@@LB@Energy -15.69 kcal/mol@LB@Hybridization Energy -34.7178 kcal/mol@LB@Unfolding Energy - mRNA 7.59764 kcal/mol@LB@Unfolding Energy - sRNA 14.4179 kcal/mol@LB@@LB@Position - mRNA 44 -- 97@LB@Position - sRNA 34 -- 84@LB@Position Seed - mRNA 90 -- 96@LB@Position Seed - sRNA 35 -- 41@LB@ |
| 75 |
0.002405 |
0.1242 |
bsu01600 |
ybbA |
-13.26 |
0.075734 |
128 -- 177 |
34 -- 77 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 12354229 2974033; Product type pe: enzyme' |
|
_____________127______________________________________________178\n_____________|________________________________________________|\n5'-UCA...CGCAG___UC_________________________GGA_______________AUAUC...UUU-3'\n______________GCA__AUUUUGGCG_________AUCC_UC____GUUCCUUAUUUCUU\n______________|||__||:|||||:_________|:||_||____:||||::|||||||\n______________CGU__UAGAACCGU_________UGGG_AG____UAAGGGGUAAAGAA\n3'-AUU...AGCAA______________UUGUUAGCA____U__AGAA______________ACUUU...CAA-5'\n_____________|________________________________________________|\n_____________78_______________________________________________33\n |
158 -- 164 |
32 -- 38 |
-31.1473 kcal/mol |
6.80918 kcal/mol |
13.8943 kcal/mol |
127 178@LB@ | |@LB@5'-UCA...CGCAG UC GGA AUAUC...UUU-3'@LB@ GCA AUUUUGGCG AUCC UC GUUCCUUAUUUCUU@LB@ ||| ||:|||||: |:|| || :||||::|||||||@LB@ CGU UAGAACCGU UGGG AG UAAGGGGUAAAGAA@LB@3'-AUU...AGCAA UUGUUAGCA U AGAA ACUUU...CAA-5'@LB@ | |@LB@ 78 33@LB@@LB@@LB@Energy -13.26 kcal/mol@LB@Hybridization Energy -31.1473 kcal/mol@LB@Unfolding Energy - mRNA 6.80918 kcal/mol@LB@Unfolding Energy - sRNA 13.8943 kcal/mol@LB@@LB@Position - mRNA 128 -- 177@LB@Position - sRNA 34 -- 77@LB@Position Seed - mRNA 158 -- 164@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 164 |
0.007653 |
0.1751 |
bsu02110 |
ybyB |
-7.82 |
0.651200 |
271 -- 286 |
38 -- 56 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________270_____287\n_____________|_______|\n5'-UUG...AAAAG_______AAAUC...AAU-3'\n______________ACCCGUC\n______________||||:||\n______________UGGGUAG\n3'-AUU...UAGCA_______AGAAU...CAA-5'\n_____________|_______|\n_____________57______37\n |
271 -- 277 |
50 -- 56 |
-11.5721 kcal/mol |
0.816393 kcal/mol |
3.06627 kcal/mol |
270 287@LB@ | |@LB@5'-UUG...AAAAG AAAUC...AAU-3'@LB@ ACCCGUC@LB@ ||||:||@LB@ UGGGUAG@LB@3'-AUU...UAGCA AGAAU...CAA-5'@LB@ | |@LB@ 57 37@LB@@LB@@LB@Energy -7.82 kcal/mol@LB@Hybridization Energy -11.5721 kcal/mol@LB@Unfolding Energy - mRNA 0.816393 kcal/mol@LB@Unfolding Energy - sRNA 3.06627 kcal/mol@LB@@LB@Position - mRNA 271 -- 286@LB@Position - sRNA 38 -- 56@LB@Position Seed - mRNA 271 -- 277@LB@Position Seed - sRNA 50 -- 56@LB@ |
| 185 |
0.008526 |
0.1751 |
bsu02370 |
ybgA |
-10.25 |
0.283898 |
252 -- 276 |
59 -- 82 |
GntR family transcriptional regulator |
|
_____________251_________________________277\n_____________|___________________________|\n5'-GAU...UUAAA___G____AAU___A____________AGCUG...UUU-3'\n______________UCG__GCA___AUC__GGCGAAUGAUC\n______________|||__|||___|||__|||:||::|||\n______________AGC__CGU___UAG__CCGUUUGUUAG\n3'-AUU...ACCGA___AA_________AA___________CAUGG...CAA-5'\n_____________|___________________________|\n_____________83__________________________58\n |
266 -- 272 |
63 -- 69 |
-19.3785 kcal/mol |
3.33361 kcal/mol |
6.42004 kcal/mol |
251 277@LB@ | |@LB@5'-GAU...UUAAA G AAU A AGCUG...UUU-3'@LB@ UCG GCA AUC GGCGAAUGAUC@LB@ ||| ||| ||| |||:||::|||@LB@ AGC CGU UAG CCGUUUGUUAG@LB@3'-AUU...ACCGA AA AA CAUGG...CAA-5'@LB@ | |@LB@ 83 58@LB@@LB@@LB@Energy -10.25 kcal/mol@LB@Hybridization Energy -19.3785 kcal/mol@LB@Unfolding Energy - mRNA 3.33361 kcal/mol@LB@Unfolding Energy - sRNA 6.42004 kcal/mol@LB@@LB@Position - mRNA 252 -- 276@LB@Position - sRNA 59 -- 82@LB@Position Seed - mRNA 266 -- 272@LB@Position Seed - sRNA 63 -- 69@LB@ |
| 68 |
0.002291 |
0.1242 |
bsu02600 |
cwlJ |
-9.38 |
0.396883 |
135 -- 166 |
88 -- 116 |
cell wall hydrolase CwlJ |
|
_____________134______________________________167\n_____________|________________________________|\n5'-UGU...UCUCC____C__CCG_____AC___U___________AUAUG...UCG-3'\n______________UGUC_CU___UCAUC__UUC_GAAGUAAUGAA\n______________||||_||___|||||__|||_:||||||||||\n______________ACAG_GA___AGUAG__AAG_UUUCAUUACUU\n3'-AUU...UUACA____U__AAG______________________ACCGA...CAA-5'\n_____________|________________________________|\n_____________117______________________________87\n |
159 -- 165 |
89 -- 95 |
-22.7508 kcal/mol |
5.74597 kcal/mol |
10.169 kcal/mol |
134 167@LB@ | |@LB@5'-UGU...UCUCC C CCG AC U AUAUG...UCG-3'@LB@ UGUC CU UCAUC UUC GAAGUAAUGAA@LB@ |||| || ||||| ||| :||||||||||@LB@ ACAG GA AGUAG AAG UUUCAUUACUU@LB@3'-AUU...UUACA U AAG ACCGA...CAA-5'@LB@ | |@LB@ 117 87@LB@@LB@@LB@Energy -9.38 kcal/mol@LB@Hybridization Energy -22.7508 kcal/mol@LB@Unfolding Energy - mRNA 5.74597 kcal/mol@LB@Unfolding Energy - sRNA 10.169 kcal/mol@LB@@LB@Position - mRNA 135 -- 166@LB@Position - sRNA 88 -- 116@LB@Position Seed - mRNA 159 -- 165@LB@Position Seed - sRNA 89 -- 95@LB@ |
| 109 |
0.004595 |
0.1633 |
bsu02700 |
estA |
-7.85 |
0.646397 |
289 -- 295 |
181 -- 187 |
lipase EstA |
bsu08350 |
_____________288_______296\n_____________|_________|\n5'-AUC...CUUAA_________AAAAA...AAC-3'\n______________AAUUUCUUU\n______________|||||||||\n______________UUAAAGAAA\n3'-AUU...GCGAA_________ACAA-5'\n_____________|_________|\n_____________188_______180\n |
109 -- 115 |
5 -- 11 |
-6.84044 kcal/mol |
1.42932 kcal/mol |
0.176622 kcal/mol |
288 296@LB@ | |@LB@5'-AUC...CUUAA AAAAA...AAC-3'@LB@ AAUUUCUUU@LB@ |||||||||@LB@ UUAAAGAAA@LB@3'-AUU...GCGAA ACAA-5'@LB@ | |@LB@ 188 180@LB@@LB@@LB@Energy -7.85 kcal/mol@LB@Hybridization Energy -6.84044 kcal/mol@LB@Unfolding Energy - mRNA 1.42932 kcal/mol@LB@Unfolding Energy - sRNA 0.176622 kcal/mol@LB@@LB@Position - mRNA 289 -- 295@LB@Position - sRNA 181 -- 187@LB@Position Seed - mRNA 109 -- 115@LB@Position Seed - sRNA 5 -- 11@LB@ |
| 183 |
0.008402 |
0.1751 |
bsu03010 |
amhX |
-13.44 |
0.069669 |
23 -- 92 |
7 -- 86 |
amidohydrolase AmhX |
|
_____________22_______________________________________________________________________________________93\n_____________|________________________________________________________________________________________|\n5'-AAA...UCUUA____G____U______GC____________________AU___GAUCCA__________________________A______UA____GAAUA...CUU-3'\n______________GGCU_UGUU_CGG_CU__UGGCA________UACUCAU__UUC______UCCCC___UCU_____GUUUGAAAAU__UUAAU__UUCU\n______________||||_:|||_|::_||__|||||________|||:|||__:||______|||||___|||_____:||||||||:__|||||__||||\n______________CCGA_GCAA_GUU_GA__ACCGU________AUGGGUA__GAG______AGGGG___AGA_____UAAACUUUUG__AAUUA__AAGA\n3'-AUU...ACUUA____A____C___A_________UUGUUAGC____________AAUA_______UAA___AACUU__________CG___________AAACAA-5'\n_____________|________________________________________________________________________________________|\n_____________87_______________________________________________________________________________________6\n |
45 -- 51 |
51 -- 57 |
-41.3184 kcal/mol |
11.5757 kcal/mol |
17.8015 kcal/mol |
22 93@LB@ | |@LB@5'-AAA...UCUUA G U GC AU GAUCCA A UA GAAUA...CUU-3'@LB@ GGCU UGUU CGG CU UGGCA UACUCAU UUC UCCCC UCU GUUUGAAAAU UUAAU UUCU@LB@ |||| :||| |:: || ||||| |||:||| :|| ||||| ||| :||||||||: ||||| ||||@LB@ CCGA GCAA GUU GA ACCGU AUGGGUA GAG AGGGG AGA UAAACUUUUG AAUUA AAGA@LB@3'-AUU...ACUUA A C A UUGUUAGC AAUA UAA AACUU CG AAACAA-5'@LB@ | |@LB@ 87 6@LB@@LB@@LB@Energy -13.44 kcal/mol@LB@Hybridization Energy -41.3184 kcal/mol@LB@Unfolding Energy - mRNA 11.5757 kcal/mol@LB@Unfolding Energy - sRNA 17.8015 kcal/mol@LB@@LB@Position - mRNA 23 -- 92@LB@Position - sRNA 7 -- 86@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 51 -- 57@LB@ |
| 93 |
0.003652 |
0.1515 |
bsu03060 |
lctP |
-13.96 |
0.054584 |
161 -- 227 |
49 -- 115 |
L-lactate permease |
bsu34190 |
_____________160_______________________________________________________________________228\n_____________|_________________________________________________________________________|\n5'-CGG...AAAAA___A___AACCG__A___________AG_______GA____U____G_______________AUG________GGAAA...UGA-3'\n______________GUC_ACU_____CA_CUUUAGAGUAA_____GGCU___UUG_CAAU__UGG_GAGCAGUUGU___AUCCGUUU\n______________|||_|||_____||_|||:|:|||||_____||||___|||_||||__|||_:|:||:|:||___|:||:|:|\n______________CAG_UGA_____GU_GAAGUUUCAUU_____CCGA___AAC_GUUA__ACC_UUUGUUAGCA___UGGGUAGA\n3'-AUU...UACAA_______AAGA___A___________ACUUA____AGC________GA___G_____________________GAAUA...CAA-5'\n_____________|_________________________________________________________________________|\n_____________116_______________________________________________________________________48\n |
220 -- 226 |
50 -- 56 |
-45.5058 kcal/mol |
15.3983 kcal/mol |
19.8755 kcal/mol |
160 228@LB@ | |@LB@5'-CGG...AAAAA A AACCG A AG GA U G AUG GGAAA...UGA-3'@LB@ GUC ACU CA CUUUAGAGUAA GGCU UUG CAAU UGG GAGCAGUUGU AUCCGUUU@LB@ ||| ||| || |||:|:||||| |||| ||| |||| ||| :|:||:|:|| |:||:|:|@LB@ CAG UGA GU GAAGUUUCAUU CCGA AAC GUUA ACC UUUGUUAGCA UGGGUAGA@LB@3'-AUU...UACAA AAGA A ACUUA AGC GA G GAAUA...CAA-5'@LB@ | |@LB@ 116 48@LB@@LB@@LB@Energy -13.96 kcal/mol@LB@Hybridization Energy -45.5058 kcal/mol@LB@Unfolding Energy - mRNA 15.3983 kcal/mol@LB@Unfolding Energy - sRNA 19.8755 kcal/mol@LB@@LB@Position - mRNA 161 -- 227@LB@Position - sRNA 49 -- 115@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 50 -- 56@LB@ |
| 189 |
0.008686 |
0.1751 |
bsu03110 |
ycgH |
-10.16 |
0.294825 |
102 -- 123 |
120 -- 141 |
transporter |
|
_____________101____________________124\n_____________|______________________|\n5'-UUU...UGAGC______UUCCUUC_________AAAGA...UCG-3'\n______________GUCUGC_______UCCAUGUAA\n______________|||:||_______|||||:|||\n______________CAGGCG_______AGGUAUAUU\n3'-AUU...UGUAA______UCGUUCA_________ACAAC...CAA-5'\n_____________|______________________|\n_____________142____________________119\n |
117 -- 123 |
120 -- 126 |
-19.5867 kcal/mol |
5.23504 kcal/mol |
6.76593 kcal/mol |
101 124@LB@ | |@LB@5'-UUU...UGAGC UUCCUUC AAAGA...UCG-3'@LB@ GUCUGC UCCAUGUAA@LB@ |||:|| |||||:|||@LB@ CAGGCG AGGUAUAUU@LB@3'-AUU...UGUAA UCGUUCA ACAAC...CAA-5'@LB@ | |@LB@ 142 119@LB@@LB@@LB@Energy -10.16 kcal/mol@LB@Hybridization Energy -19.5867 kcal/mol@LB@Unfolding Energy - mRNA 5.23504 kcal/mol@LB@Unfolding Energy - sRNA 6.76593 kcal/mol@LB@@LB@Position - mRNA 102 -- 123@LB@Position - sRNA 120 -- 141@LB@Position Seed - mRNA 117 -- 123@LB@Position Seed - sRNA 120 -- 126@LB@ |
