Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
148	0.01095	0.2504	b0082	rsmH	-17.59	0.005541	130 -- 156	1 -- 25	16S rRNA m(4)C1402 methyltransferase SAM-dependent	_____________129_________________________157\n_____________\|___________________________\|\n5'-UGG...GUCAA__AA________C_C________U___UACCG...GUC-3'\n______________GG__GAUAUCGA_G_AGAGCAGU_GGC\n______________\|\|__\|\|:\|\|\|:\|_\|_\|:\|\|\|\|\|\|_\|\|\|\n______________CC__CUGUAGUU_C_UUUCGUCA_CCG\n3'-UUU...UUUUA__C_________U_U____________-5'\n_____________\|__________________________\|\n_____________26_________________________1\n	146 -- 152	4 -- 10	-26.6505 kcal/mol	6.5392 kcal/mol	2.51748 kcal/mol	129 157@LB@ \| \|@LB@5'-UGG...GUCAA AA C C U UACCG...GUC-3'@LB@ GG GAUAUCGA G AGAGCAGU GGC@LB@ \|\| \|\|:\|\|\|:\| \| \|:\|\|\|\|\|\| \|\|\|@LB@ CC CUGUAGUU C UUUCGUCA CCG@LB@3'-UUU...UUUUA C U U -5'@LB@ \| \|@LB@ 26 1@LB@@LB@@LB@Energy -17.59 kcal/mol@LB@Hybridization Energy -26.6505 kcal/mol@LB@Unfolding Energy - mRNA 6.5392 kcal/mol@LB@Unfolding Energy - sRNA 2.51748 kcal/mol@LB@@LB@Position - mRNA 130 -- 156@LB@Position - sRNA 1 -- 25@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 4 -- 10@LB@
99	0.006147	0.2179	b4477	dgoA	-14.15	0.044006	176 -- 210	1 -- 31	2-oxo-3-deoxygalactonate 6-phosphate aldolase	_____________175___________________________________211\n_____________\|_____________________________________\|\n5'-CAG...CAUUU____C___UAUAA___G_____CUCAU__________AAACU...UCG-3'\n______________CAGG_UGG_____GGA_CAUCG_______GCAGUGGC\n______________\|\|::_\|\|\|_____\|\|\|_\|\|\|\|:_______\|\|\|\|\|\|\|\|\n______________GUUU_ACC_____CCU_GUAGU_______CGUCACCG\n3'-UUU...GAGGU____U_________________UUCUUUU________-5'\n_____________\|____________________________________\|\n_____________32___________________________________1\n	204 -- 210	1 -- 7	-26.5297 kcal/mol	9.84206 kcal/mol	2.53929 kcal/mol	175 211@LB@ \| \|@LB@5'-CAG...CAUUU C UAUAA G CUCAU AAACU...UCG-3'@LB@ CAGG UGG GGA CAUCG GCAGUGGC@LB@ \|\|:: \|\|\| \|\|\| \|\|\|\|: \|\|\|\|\|\|\|\|@LB@ GUUU ACC CCU GUAGU CGUCACCG@LB@3'-UUU...GAGGU U UUCUUUU -5'@LB@ \| \|@LB@ 32 1@LB@@LB@@LB@Energy -14.15 kcal/mol@LB@Hybridization Energy -26.5297 kcal/mol@LB@Unfolding Energy - mRNA 9.84206 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 176 -- 210@LB@Position - sRNA 1 -- 31@LB@Position Seed - mRNA 204 -- 210@LB@Position Seed - sRNA 1 -- 7@LB@
79	0.004643	0.2062	b2264	menD	-12.62	0.093644	52 -- 86	1 -- 30	2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase; SEPHCHC synthase	_____________51_____________________________________87\n_____________\|______________________________________\|\n5'-CGU...GAGCA_______CAGGAAAUC____A_____C___________UGCGU...GUU-3'\n______________AGAGUGG_________GACA_CAAAG___GGCAG_GGC\n______________\|:\|:\|\|\|_________\|\|\|\|_\|\|\|\|\|___:\|\|\|\|_\|\|\|\n______________UUUUACC_________CUGU_GUUUC___UCGUC_CCG\n3'-UUU...AGGUG_______C____________A_____UUU_____A___-5'\n_____________\|_____________________________________\|\n_____________31____________________________________1\n	52 -- 58	24 -- 30	-24.8101 kcal/mol	9.6737 kcal/mol	2.51911 kcal/mol	51 87@LB@ \| \|@LB@5'-CGU...GAGCA CAGGAAAUC A C UGCGU...GUU-3'@LB@ AGAGUGG GACA CAAAG GGCAG GGC@LB@ \|:\|:\|\|\| \|\|\|\| \|\|\|\|\| :\|\|\|\| \|\|\|@LB@ UUUUACC CUGU GUUUC UCGUC CCG@LB@3'-UUU...AGGUG C A UUU A -5'@LB@ \| \|@LB@ 31 1@LB@@LB@@LB@Energy -12.62 kcal/mol@LB@Hybridization Energy -24.8101 kcal/mol@LB@Unfolding Energy - mRNA 9.6737 kcal/mol@LB@Unfolding Energy - sRNA 2.51911 kcal/mol@LB@@LB@Position - mRNA 52 -- 86@LB@Position - sRNA 1 -- 30@LB@Position Seed - mRNA 52 -- 58@LB@Position Seed - sRNA 24 -- 30@LB@
101	0.006298	0.2188	b3549	tag	-14.94	0.028658	147 -- 165	3 -- 21	3-methyl-adenine DNA glycosylase I constitutive	_____________146__________________166\n_____________\|____________________\|\n5'-GCA...CCGAU_________________C__AAAUU...AAA-3'\n______________GCGUC_AAGAAAAGCGG_UG\n______________:\|:\|\|_\|\|\|\|\|\|\|\|\|:\|_\|\|\n______________UGUAG_UUCUUUUCGUC_AC\n3'-UUU...ACCCC_____U______________CG-5'\n_____________\|____________________\|\n_____________22___________________2\n	156 -- 162	5 -- 11	-20.7072 kcal/mol	4.25403 kcal/mol	1.5107 kcal/mol	146 166@LB@ \| \|@LB@5'-GCA...CCGAU C AAAUU...AAA-3'@LB@ GCGUC AAGAAAAGCGG UG@LB@ :\|:\|\| \|\|\|\|\|\|\|\|\|:\| \|\|@LB@ UGUAG UUCUUUUCGUC AC@LB@3'-UUU...ACCCC U CG-5'@LB@ \| \|@LB@ 22 2@LB@@LB@@LB@Energy -14.94 kcal/mol@LB@Hybridization Energy -20.7072 kcal/mol@LB@Unfolding Energy - mRNA 4.25403 kcal/mol@LB@Unfolding Energy - sRNA 1.5107 kcal/mol@LB@@LB@Position - mRNA 147 -- 165@LB@Position - sRNA 3 -- 21@LB@Position Seed - mRNA 156 -- 162@LB@Position Seed - sRNA 5 -- 11@LB@
149	0.01096	0.2504	b3230	rpsI	-12.23	0.111717	165 -- 205	1 -- 34	30S ribosomal subunit protein S9	_____________164_______________________________________206\n_____________\|_________________________________________\|\n5'-GUG...AGCAA_______UUCUU______UAAUC____UUAU_____A____UGAAA...GUU-3'\n______________CCGCAAG_____GACAUC_____GGGA____AGGCA_UGGC\n______________\|\|:\|\|\|:_____\|\|\|\|\|\|_____:\|:\|____\|:\|\|\|_\|\|\|\|\n______________GGUGUUU_____CUGUAG_____UCUU____UUCGU_ACCG\n3'-UUU...CCCGA_______UACCC______UU________________C____-5'\n_____________\|________________________________________\|\n_____________35_______________________________________1\n	165 -- 171	28 -- 34	-26.3465 kcal/mol	9.84752 kcal/mol	4.26884 kcal/mol	164 206@LB@ \| \|@LB@5'-GUG...AGCAA UUCUU UAAUC UUAU A UGAAA...GUU-3'@LB@ CCGCAAG GACAUC GGGA AGGCA UGGC@LB@ \|\|:\|\|\|: \|\|\|\|\|\| :\|:\| \|:\|\|\| \|\|\|\|@LB@ GGUGUUU CUGUAG UCUU UUCGU ACCG@LB@3'-UUU...CCCGA UACCC UU C -5'@LB@ \| \|@LB@ 35 1@LB@@LB@@LB@Energy -12.23 kcal/mol@LB@Hybridization Energy -26.3465 kcal/mol@LB@Unfolding Energy - mRNA 9.84752 kcal/mol@LB@Unfolding Energy - sRNA 4.26884 kcal/mol@LB@@LB@Position - mRNA 165 -- 205@LB@Position - sRNA 1 -- 34@LB@Position Seed - mRNA 165 -- 171@LB@Position Seed - sRNA 28 -- 34@LB@
55	0.002823	0.1778	b3319	rplD	-12.54	0.096993	18 -- 45	1 -- 32	50S ribosomal subunit protein L4	_____________17________________________________46\n_____________\|_________________________________\|\n5'-UCA...UCCGG________U____________C___AAAA____AGGUC...UUG-3'\n______________GCAAAGUG______UCAAAGG_AAG____UGGC\n______________:\|\|\|\|:\|\|______\|\|\|\|\|\|:_\|\|\|____\|\|\|\|\n______________UGUUUUAC______AGUUUCU_UUC____ACCG\n3'-UUU...CGAGG________CCCUGU_______U___GUC_____-5'\n_____________\|________________________________\|\n_____________33_______________________________1\n	27 -- 33	12 -- 18	-19.13 kcal/mol	4.04955 kcal/mol	2.53929 kcal/mol	17 46@LB@ \| \|@LB@5'-UCA...UCCGG U C AAAA AGGUC...UUG-3'@LB@ GCAAAGUG UCAAAGG AAG UGGC@LB@ :\|\|\|\|:\|\| \|\|\|\|\|\|: \|\|\| \|\|\|\|@LB@ UGUUUUAC AGUUUCU UUC ACCG@LB@3'-UUU...CGAGG CCCUGU U GUC -5'@LB@ \| \|@LB@ 33 1@LB@@LB@@LB@Energy -12.54 kcal/mol@LB@Hybridization Energy -19.13 kcal/mol@LB@Unfolding Energy - mRNA 4.04955 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 18 -- 45@LB@Position - sRNA 1 -- 32@LB@Position Seed - mRNA 27 -- 33@LB@Position Seed - sRNA 12 -- 18@LB@
160	0.0118	0.2504	b0078	ilvH	-13.58	0.059018	151 -- 175	1 -- 25	acetolactate synthase 3 small subunit valine-sensitive	_____________150_______________________176\n_____________\|_________________________\|\n5'-GCC...UUCGC_______GAAU___UGAAAU_____UAAGC...UGA-3'\n______________GGGGGCG____GGA______GUGGU\n______________\|\|\|\|:\|:____:\|\|______\|\|\|\|:\n______________CCCCUGU____UCU______CACCG\n3'-UUU...UUUUA_______AGUU___UUUCGU_____-5'\n_____________\|________________________\|\n_____________26_______________________1\n	151 -- 157	19 -- 25	-21.9405 kcal/mol	5.84786 kcal/mol	2.51748 kcal/mol	150 176@LB@ \| \|@LB@5'-GCC...UUCGC GAAU UGAAAU UAAGC...UGA-3'@LB@ GGGGGCG GGA GUGGU@LB@ \|\|\|\|:\|: :\|\| \|\|\|\|:@LB@ CCCCUGU UCU CACCG@LB@3'-UUU...UUUUA AGUU UUUCGU -5'@LB@ \| \|@LB@ 26 1@LB@@LB@@LB@Energy -13.58 kcal/mol@LB@Hybridization Energy -21.9405 kcal/mol@LB@Unfolding Energy - mRNA 5.84786 kcal/mol@LB@Unfolding Energy - sRNA 2.51748 kcal/mol@LB@@LB@Position - mRNA 151 -- 175@LB@Position - sRNA 1 -- 25@LB@Position Seed - mRNA 151 -- 157@LB@Position Seed - sRNA 19 -- 25@LB@
179	0.01303	0.2504	b1276	acnA	-14.81	0.030801	184 -- 199	7 -- 22	aconitate hydratase 1; aconitase A	_____________183______________200\n_____________\|________________\|\n5'-CUU...ACCCU_U______________UAUGU...GCG-3'\n______________G_CAUUAAGGAGGAGC\n______________\|_\|\|\|:\|\|:\|\|::\|\|\|\n______________C_GUAGUUUCUUUUCG\n3'-UUU...UACCC_U______________UCACCG-5'\n_____________\|________________\|\n_____________23_______________6\n	193 -- 199	7 -- 13	-17.7225 kcal/mol	2.31939 kcal/mol	0.590875 kcal/mol	183 200@LB@ \| \|@LB@5'-CUU...ACCCU U UAUGU...GCG-3'@LB@ G CAUUAAGGAGGAGC@LB@ \| \|\|\|:\|\|:\|\|::\|\|\|@LB@ C GUAGUUUCUUUUCG@LB@3'-UUU...UACCC U UCACCG-5'@LB@ \| \|@LB@ 23 6@LB@@LB@@LB@Energy -14.81 kcal/mol@LB@Hybridization Energy -17.7225 kcal/mol@LB@Unfolding Energy - mRNA 2.31939 kcal/mol@LB@Unfolding Energy - sRNA 0.590875 kcal/mol@LB@@LB@Position - mRNA 184 -- 199@LB@Position - sRNA 7 -- 22@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 7 -- 13@LB@
200	0.01506	0.259	b0404	acpH	-14.03	0.046767	57 -- 80	6 -- 28	acyl carrier protein (ACP) phosphodiesterase; ACP hydrolyase	_____________56________________________81\n_____________\|_________________________\|\n5'-GCC...GGGAU______CA_________UUC_____AUUCA...GUU-3'\n______________AGUGGG___AUCAGGGA___GGGCA\n______________\|:\|\|\|\|___\|\|\|\|::\|\|___::\|\|\|\n______________UUACCC___UAGUUUCU___UUCGU\n3'-UUU...GUGUU______CUG________U_______CACCG-5'\n_____________\|_________________________\|\n_____________29________________________5\n	66 -- 72	12 -- 18	-19.5231 kcal/mol	4.78501 kcal/mol	0.706454 kcal/mol	56 81@LB@ \| \|@LB@5'-GCC...GGGAU CA UUC AUUCA...GUU-3'@LB@ AGUGGG AUCAGGGA GGGCA@LB@ \|:\|\|\|\| \|\|\|\|::\|\| ::\|\|\|@LB@ UUACCC UAGUUUCU UUCGU@LB@3'-UUU...GUGUU CUG U CACCG-5'@LB@ \| \|@LB@ 29 5@LB@@LB@@LB@Energy -14.03 kcal/mol@LB@Hybridization Energy -19.5231 kcal/mol@LB@Unfolding Energy - mRNA 4.78501 kcal/mol@LB@Unfolding Energy - sRNA 0.706454 kcal/mol@LB@@LB@Position - mRNA 57 -- 80@LB@Position - sRNA 6 -- 28@LB@Position Seed - mRNA 66 -- 72@LB@Position Seed - sRNA 12 -- 18@LB@
92	0.005693	0.2147	b2099	yegU	-12.49	0.099484	183 -- 197	7 -- 22	ADP-ribosylglycohydrolase family protein	_____________182______________198\n_____________\|________________\|\n5'-GUG...GCGUU________________UAAAU...CAC-3'\n______________GACA_CAAGGGGAAGU\n______________\|\|\|\|_\|\|\|:\|::\|\|\|:\n______________CUGU_GUUUCUUUUCG\n3'-UUU...UACCC____A___________UCACCG-5'\n_____________\|________________\|\n_____________23_______________6\n	191 -- 197	7 -- 13	-15.6094 kcal/mol	2.53245 kcal/mol	0.590875 kcal/mol	182 198@LB@ \| \|@LB@5'-GUG...GCGUU UAAAU...CAC-3'@LB@ GACA CAAGGGGAAGU@LB@ \|\|\|\| \|\|\|:\|::\|\|\|:@LB@ CUGU GUUUCUUUUCG@LB@3'-UUU...UACCC A UCACCG-5'@LB@ \| \|@LB@ 23 6@LB@@LB@@LB@Energy -12.49 kcal/mol@LB@Hybridization Energy -15.6094 kcal/mol@LB@Unfolding Energy - mRNA 2.53245 kcal/mol@LB@Unfolding Energy - sRNA 0.590875 kcal/mol@LB@@LB@Position - mRNA 183 -- 197@LB@Position - sRNA 7 -- 22@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 7 -- 13@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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usability assessment

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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