Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
117	0.003878	0.1165	b3078	ygjI	-10.25	0.038289	174 -- 181	1 -- 8	putative transporter	b0899	_____________173______182\n_____________\|________\|\n5'-GGA...ACCGC________UCAUU...UUG-3'\n______________UACCCUAC\n______________\|\|\|\|\|\|\|\|\n______________AUGGGAUG\n3'-AUU...GAGAC________-5'\n_____________\|_______\|\n_____________9_______1\n	175 -- 181	1 -- 7	-12.7576 kcal/mol	0.416118 kcal/mol	2.11603 kcal/mol	173 182@LB@ \| \|@LB@5'-GGA...ACCGC UCAUU...UUG-3'@LB@ UACCCUAC@LB@ \|\|\|\|\|\|\|\|@LB@ AUGGGAUG@LB@3'-AUU...GAGAC -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -10.25 kcal/mol@LB@Hybridization Energy -12.7576 kcal/mol@LB@Unfolding Energy - mRNA 0.416118 kcal/mol@LB@Unfolding Energy - sRNA 2.11603 kcal/mol@LB@@LB@Position - mRNA 174 -- 181@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 175 -- 181@LB@Position Seed - sRNA 1 -- 7@LB@
2	0	0	b0796	ybiH	-7.52	0.201543	119 -- 140	54 -- 76	DUF1956 domain-containing tetR family putative transcriptional regulator	b1013	_____________118_____________________141\n_____________\|_______________________\|\n5'-CGG...GCGUU________UC_______UA____GACAA...CCA-3'\n______________GCUAAAAU__UUAGCCA___CUG\n______________\|\|\|\|\|\|\|\|__\|:\|\|\|\|\|___\|:\|\n______________CGAUUUUA__AGUCGGU___GGC\n3'-AUU...CCCGC________UA_______UUA___UAAUG...AUG-5'\n_____________\|_______________________\|\n_____________77______________________53\n	129 -- 135	60 -- 66	-19.7488 kcal/mol	5.88928 kcal/mol	6.3634 kcal/mol	118 141@LB@ \| \|@LB@5'-CGG...GCGUU UC UA GACAA...CCA-3'@LB@ GCUAAAAU UUAGCCA CUG@LB@ \|\|\|\|\|\|\|\| \|:\|\|\|\|\| \|:\|@LB@ CGAUUUUA AGUCGGU GGC@LB@3'-AUU...CCCGC UA UUA UAAUG...AUG-5'@LB@ \| \|@LB@ 77 53@LB@@LB@@LB@Energy -7.52 kcal/mol@LB@Hybridization Energy -19.7488 kcal/mol@LB@Unfolding Energy - mRNA 5.88928 kcal/mol@LB@Unfolding Energy - sRNA 6.3634 kcal/mol@LB@@LB@Position - mRNA 119 -- 140@LB@Position - sRNA 54 -- 76@LB@Position Seed - mRNA 129 -- 135@LB@Position Seed - sRNA 60 -- 66@LB@
26	0.0002109	0.02687	b4122	fumB	-13.50	0.002989	276 -- 286	47 -- 57	anaerobic class I fumarate hydratase (fumarase B)	b1612	_____________275_________287\n_____________\|___________\|\n5'-UCC...UCACU___________UAGCG...ACU-3'\n______________UCCGAUUACGU\n______________\|\|\|\|\|\|\|\|\|\|\|\n______________AGGCUAAUGCA\n3'-AUU...GGUUU___________CUUCA...AUG-5'\n_____________\|___________\|\n_____________58__________46\n	280 -- 286	47 -- 53	-16.5827 kcal/mol	1.51899 kcal/mol	1.56839 kcal/mol	275 287@LB@ \| \|@LB@5'-UCC...UCACU UAGCG...ACU-3'@LB@ UCCGAUUACGU@LB@ \|\|\|\|\|\|\|\|\|\|\|@LB@ AGGCUAAUGCA@LB@3'-AUU...GGUUU CUUCA...AUG-5'@LB@ \| \|@LB@ 58 46@LB@@LB@@LB@Energy -13.50 kcal/mol@LB@Hybridization Energy -16.5827 kcal/mol@LB@Unfolding Energy - mRNA 1.51899 kcal/mol@LB@Unfolding Energy - sRNA 1.56839 kcal/mol@LB@@LB@Position - mRNA 276 -- 286@LB@Position - sRNA 47 -- 57@LB@Position Seed - mRNA 280 -- 286@LB@Position Seed - sRNA 47 -- 53@LB@
53	0.0007581	0.04961	b1302	puuE	-16.93	0.000076	166 -- 207	20 -- 57	4-aminobutyrate aminotransferase PLP-dependent	b2662	_____________165_________________________________________208\n_____________\|___________________________________________\|\n5'-AGC...UAAUU________AC_______AGUC_______G_____C________ACAAU...CGC-3'\n______________UCCGAUUA__CGUGAAG____AAAAGGU_UGAAA_AU_GAGCA\n______________\|\|\|\|\|\|\|\|__\|\|\|\|\|\|\|____\|\|\|\|\|\|\|_\|\|\|\|\|_\|\|_\|\|:\|\|\n______________AGGCUAAU__GCACUUC____UUUUCCA_ACUUU_UA_CUUGU\n3'-AUU...GGUUU_________________A__________G_____C__U_____AGAAU...AUG-5'\n_____________\|___________________________________________\|\n_____________58__________________________________________19\n	176 -- 182	43 -- 49	-36.956 kcal/mol	7.85943 kcal/mol	12.1551 kcal/mol	165 208@LB@ \| \|@LB@5'-AGC...UAAUU AC AGUC G C ACAAU...CGC-3'@LB@ UCCGAUUA CGUGAAG AAAAGGU UGAAA AU GAGCA@LB@ \|\|\|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|\|\| \|\| \|\|:\|\|@LB@ AGGCUAAU GCACUUC UUUUCCA ACUUU UA CUUGU@LB@3'-AUU...GGUUU A G C U AGAAU...AUG-5'@LB@ \| \|@LB@ 58 19@LB@@LB@@LB@Energy -16.93 kcal/mol@LB@Hybridization Energy -36.956 kcal/mol@LB@Unfolding Energy - mRNA 7.85943 kcal/mol@LB@Unfolding Energy - sRNA 12.1551 kcal/mol@LB@@LB@Position - mRNA 166 -- 207@LB@Position - sRNA 20 -- 57@LB@Position Seed - mRNA 176 -- 182@LB@Position Seed - sRNA 43 -- 49@LB@
163	0.006545	0.1413	b0532	sfmD	-7.71	0.181786	104 -- 132	48 -- 76	putative outer membrane export usher protein	b3144 b4317 b0940	_____________103____________________________133\n_____________\|______________________________\|\n5'-UCA...GGAAA______________A___UUAA________GUUCA...GCU-3'\n______________GCUGAAAU_UUCAG_CCG____UGAUUAUG\n______________\|\|\|:\|\|\|\|_\|\|\|\|\|_\|\|:____:\|\|\|\|\|:\|\n______________CGAUUUUA_AAGUC_GGU____GCUAAUGC\n3'-AUU...CCCGC________U_________UUAG________ACUUC...AUG-5'\n_____________\|______________________________\|\n_____________77_____________________________47\n	126 -- 132	48 -- 54	-23.9612 kcal/mol	8.59534 kcal/mol	7.67378 kcal/mol	103 133@LB@ \| \|@LB@5'-UCA...GGAAA A UUAA GUUCA...GCU-3'@LB@ GCUGAAAU UUCAG CCG UGAUUAUG@LB@ \|\|\|:\|\|\|\| \|\|\|\|\| \|\|: :\|\|\|\|\|:\|@LB@ CGAUUUUA AAGUC GGU GCUAAUGC@LB@3'-AUU...CCCGC U UUAG ACUUC...AUG-5'@LB@ \| \|@LB@ 77 47@LB@@LB@@LB@Energy -7.71 kcal/mol@LB@Hybridization Energy -23.9612 kcal/mol@LB@Unfolding Energy - mRNA 8.59534 kcal/mol@LB@Unfolding Energy - sRNA 7.67378 kcal/mol@LB@@LB@Position - mRNA 104 -- 132@LB@Position - sRNA 48 -- 76@LB@Position Seed - mRNA 126 -- 132@LB@Position Seed - sRNA 48 -- 54@LB@
158	0.00634	0.1412	b3598	yibI	-10.19	0.039756	289 -- 298	49 -- 58	DUF3302 family inner membrane protein	b3587	_____________288________299\n_____________\|__________\|\n5'-CGU...CCCUU__________CA-3'\n______________AUCUGAUUGC\n______________\|\|\|:\|\|\|\|:\|\n______________UAGGCUAAUG\n3'-AUU...CGGUU__________CACUU...AUG-5'\n_____________\|__________\|\n_____________59_________48\n	292 -- 298	49 -- 55	-10.9433 kcal/mol	0.440755 kcal/mol	0.318367 kcal/mol	288 299@LB@ \| \|@LB@5'-CGU...CCCUU CA-3'@LB@ AUCUGAUUGC@LB@ \|\|\|:\|\|\|\|:\|@LB@ UAGGCUAAUG@LB@3'-AUU...CGGUU CACUU...AUG-5'@LB@ \| \|@LB@ 59 48@LB@@LB@@LB@Energy -10.19 kcal/mol@LB@Hybridization Energy -10.9433 kcal/mol@LB@Unfolding Energy - mRNA 0.440755 kcal/mol@LB@Unfolding Energy - sRNA 0.318367 kcal/mol@LB@@LB@Position - mRNA 289 -- 298@LB@Position - sRNA 49 -- 58@LB@Position Seed - mRNA 292 -- 298@LB@Position Seed - sRNA 49 -- 55@LB@
88	0.002339	0.09349	b3878	yihQ	-11.00	0.022508	260 -- 267	1 -- 8	putative sulpholipid alpha-glucosidase; alpha-glucosyl fluoride glucosidase	b3656	_____________259______268\n_____________\|________\|\n5'-AAC...AGUUU________AUUUU...GCA-3'\n______________UACCCUAC\n______________\|\|\|\|\|\|\|\|\n______________AUGGGAUG\n3'-AUU...GAGAC________-5'\n_____________\|_______\|\n_____________9_______1\n	261 -- 267	1 -- 7	-13.427 kcal/mol	0.33456 kcal/mol	2.11603 kcal/mol	259 268@LB@ \| \|@LB@5'-AAC...AGUUU AUUUU...GCA-3'@LB@ UACCCUAC@LB@ \|\|\|\|\|\|\|\|@LB@ AUGGGAUG@LB@3'-AUU...GAGAC -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -11.00 kcal/mol@LB@Hybridization Energy -13.427 kcal/mol@LB@Unfolding Energy - mRNA 0.33456 kcal/mol@LB@Unfolding Energy - sRNA 2.11603 kcal/mol@LB@@LB@Position - mRNA 260 -- 267@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 261 -- 267@LB@Position Seed - sRNA 1 -- 7@LB@
175	0.007291	0.1466	b1498	ydeN	-8.19	0.139530	95 -- 124	45 -- 67	putative Ser-type periplasmic non-aryl sulfatase	b3678 b3801	_____________94_____________________________125\n_____________\|______________________________\|\n5'-AUU...CUUUU______________CUAUCUCU________CUAAC...UAA-3'\n______________UUUAGCUAAAUCUG________UUAUGUGA\n______________\|\|:\|\|\|:\|\|\|\|\|:\|________\|\|\|:\|\|\|\|\n______________AAGUCGGUUUAGGC________AAUGCACU\n3'-AUU...UUUAU______________U_______________UCAUU...AUG-5'\n_____________\|______________________________\|\n_____________68_____________________________44\n	118 -- 124	45 -- 51	-22.2428 kcal/mol	5.90754 kcal/mol	8.16749 kcal/mol	94 125@LB@ \| \|@LB@5'-AUU...CUUUU CUAUCUCU CUAAC...UAA-3'@LB@ UUUAGCUAAAUCUG UUAUGUGA@LB@ \|\|:\|\|\|:\|\|\|\|\|:\| \|\|\|:\|\|\|\|@LB@ AAGUCGGUUUAGGC AAUGCACU@LB@3'-AUU...UUUAU U UCAUU...AUG-5'@LB@ \| \|@LB@ 68 44@LB@@LB@@LB@Energy -8.19 kcal/mol@LB@Hybridization Energy -22.2428 kcal/mol@LB@Unfolding Energy - mRNA 5.90754 kcal/mol@LB@Unfolding Energy - sRNA 8.16749 kcal/mol@LB@@LB@Position - mRNA 95 -- 124@LB@Position - sRNA 45 -- 67@LB@Position Seed - mRNA 118 -- 124@LB@Position Seed - sRNA 45 -- 51@LB@
191	0.008587	0.1558	b0823	ybiW	-9.49	0.063221	47 -- 54	1 -- 8	putative pyruvate formate lyase	b3951	_____________46_______55\n_____________\|________\|\n5'-ACC...UUUUC________CACAC...CGC-3'\n______________UGCCCUAC\n______________\|:\|\|\|\|\|\|\n______________AUGGGAUG\n3'-AUU...GAGAC________-5'\n_____________\|_______\|\n_____________9_______1\n	48 -- 54	1 -- 7	-12.4662 kcal/mol	0.878635 kcal/mol	2.11603 kcal/mol	46 55@LB@ \| \|@LB@5'-ACC...UUUUC CACAC...CGC-3'@LB@ UGCCCUAC@LB@ \|:\|\|\|\|\|\|@LB@ AUGGGAUG@LB@3'-AUU...GAGAC -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -9.49 kcal/mol@LB@Hybridization Energy -12.4662 kcal/mol@LB@Unfolding Energy - mRNA 0.878635 kcal/mol@LB@Unfolding Energy - sRNA 2.11603 kcal/mol@LB@@LB@Position - mRNA 47 -- 54@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 48 -- 54@LB@Position Seed - sRNA 1 -- 7@LB@
79	0.001698	0.07533	b2890	lysS	-8.16	0.142186	53 -- 64	54 -- 65	lysine tRNA synthetase constitutive	b4129	_____________52___________65\n_____________\|____________\|\n5'-AAA...UGGGG____________UUCUU...GGA-3'\n______________CAGCCAGAUUCG\n______________\|\|\|\|\|\|:\|\|:\|\|\n______________GUCGGUUUAGGC\n3'-AUU...UAUAA____________UAAUG...AUG-5'\n_____________\|____________\|\n_____________66___________53\n	58 -- 64	54 -- 60	-18.439 kcal/mol	4.14677 kcal/mol	6.15795 kcal/mol	52 65@LB@ \| \|@LB@5'-AAA...UGGGG UUCUU...GGA-3'@LB@ CAGCCAGAUUCG@LB@ \|\|\|\|\|\|:\|\|:\|\|@LB@ GUCGGUUUAGGC@LB@3'-AUU...UAUAA UAAUG...AUG-5'@LB@ \| \|@LB@ 66 53@LB@@LB@@LB@Energy -8.16 kcal/mol@LB@Hybridization Energy -18.439 kcal/mol@LB@Unfolding Energy - mRNA 4.14677 kcal/mol@LB@Unfolding Energy - sRNA 6.15795 kcal/mol@LB@@LB@Position - mRNA 53 -- 64@LB@Position - sRNA 54 -- 65@LB@Position Seed - mRNA 58 -- 64@LB@Position Seed - sRNA 54 -- 60@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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