Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
73	0.001217	0.05856	b1702	ppsA	-8.47	0.118779	124 -- 132	50 -- 58	phosphoenolpyruvate synthase	_____________123_______133\n_____________\|_________\|\n5'-UAU...UUUUC_________AAUAU...AAA-3'\n______________AUCCGGUUA\n______________\|\|\|\|\|:\|\|\|\n______________UAGGCUAAU\n3'-AUU...CGGUU_________GCACU...AUG-5'\n_____________\|_________\|\n_____________59________49\n	126 -- 132	50 -- 56	-10.3571 kcal/mol	1.58803 kcal/mol	0.317553 kcal/mol	123 133@LB@ \| \|@LB@5'-UAU...UUUUC AAUAU...AAA-3'@LB@ AUCCGGUUA@LB@ \|\|\|\|\|:\|\|\|@LB@ UAGGCUAAU@LB@3'-AUU...CGGUU GCACU...AUG-5'@LB@ \| \|@LB@ 59 49@LB@@LB@@LB@Energy -8.47 kcal/mol@LB@Hybridization Energy -10.3571 kcal/mol@LB@Unfolding Energy - mRNA 1.58803 kcal/mol@LB@Unfolding Energy - sRNA 0.317553 kcal/mol@LB@@LB@Position - mRNA 124 -- 132@LB@Position - sRNA 50 -- 58@LB@Position Seed - mRNA 126 -- 132@LB@Position Seed - sRNA 50 -- 56@LB@
171	0.00718	0.1466	b1708	nlpC	-9.44	0.065343	288 -- 298	49 -- 59	putative C40 clan peptidase lipoprotein	_____________287_________299\n_____________\|___________\|\n5'-AAA...UUUCU___________CG-3'\n______________GAUUCGAUUAC\n______________:\|\|:\|\|\|\|\|\|\|\n______________UUAGGCUAAUG\n3'-AUU...UCGGU___________CACUU...AUG-5'\n_____________\|___________\|\n_____________60__________48\n	292 -- 298	49 -- 55	-11.5278 kcal/mol	1.77565 kcal/mol	0.318405 kcal/mol	287 299@LB@ \| \|@LB@5'-AAA...UUUCU CG-3'@LB@ GAUUCGAUUAC@LB@ :\|\|:\|\|\|\|\|\|\|@LB@ UUAGGCUAAUG@LB@3'-AUU...UCGGU CACUU...AUG-5'@LB@ \| \|@LB@ 60 48@LB@@LB@@LB@Energy -9.44 kcal/mol@LB@Hybridization Energy -11.5278 kcal/mol@LB@Unfolding Energy - mRNA 1.77565 kcal/mol@LB@Unfolding Energy - sRNA 0.318405 kcal/mol@LB@@LB@Position - mRNA 288 -- 298@LB@Position - sRNA 49 -- 59@LB@Position Seed - mRNA 292 -- 298@LB@Position Seed - sRNA 49 -- 55@LB@
46	0.0005017	0.03778	b1713	pheT	-7.80	0.173677	95 -- 107	17 -- 29	phenylalanine tRNA synthetase beta subunit	_____________94____________108\n_____________\|_____________\|\n5'-CUG...GAUGG_____________GACUA...ACG-3'\n______________GGAUGGAGCGUCU\n______________:\|\|\|:\|\|:\|:\|\|\|\n______________UCUAUCUUGUAGA\n3'-AUU...GACUU_____________AUGGA...AUG-5'\n_____________\|_____________\|\n_____________30____________16\n	100 -- 106	18 -- 24	-17.5948 kcal/mol	5.55471 kcal/mol	4.26164 kcal/mol	94 108@LB@ \| \|@LB@5'-CUG...GAUGG GACUA...ACG-3'@LB@ GGAUGGAGCGUCU@LB@ :\|\|\|:\|\|:\|:\|\|\|@LB@ UCUAUCUUGUAGA@LB@3'-AUU...GACUU AUGGA...AUG-5'@LB@ \| \|@LB@ 30 16@LB@@LB@@LB@Energy -7.80 kcal/mol@LB@Hybridization Energy -17.5948 kcal/mol@LB@Unfolding Energy - mRNA 5.55471 kcal/mol@LB@Unfolding Energy - sRNA 4.26164 kcal/mol@LB@@LB@Position - mRNA 95 -- 107@LB@Position - sRNA 17 -- 29@LB@Position Seed - mRNA 100 -- 106@LB@Position Seed - sRNA 18 -- 24@LB@
113	0.003703	0.1147	b1728	ydjM	-2.69	0.937889	161 -- 167	1 -- 7	inner membrane protein regulated by LexA	_____________160_____168\n_____________\|_______\|\n5'-CGC...UAAAA_______ACUGU...AUA-3'\n______________AUCCUAU\n______________\|:\|\|\|\|:\n______________UGGGAUG\n3'-AUU...AGACA_______-5'\n_____________\|______\|\n_____________8______1\n	161 -- 167	1 -- 7	-7.15055 kcal/mol	2.44298 kcal/mol	2.03949 kcal/mol	160 168@LB@ \| \|@LB@5'-CGC...UAAAA ACUGU...AUA-3'@LB@ AUCCUAU@LB@ \|:\|\|\|\|:@LB@ UGGGAUG@LB@3'-AUU...AGACA -5'@LB@ \| \|@LB@ 8 1@LB@@LB@@LB@Energy -2.69 kcal/mol@LB@Hybridization Energy -7.15055 kcal/mol@LB@Unfolding Energy - mRNA 2.44298 kcal/mol@LB@Unfolding Energy - sRNA 2.03949 kcal/mol@LB@@LB@Position - mRNA 161 -- 167@LB@Position - sRNA 1 -- 7@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 1 -- 7@LB@
121	0.004045	0.1176	b1738	chbB	-6.64	0.311906	2 -- 15	34 -- 50	N N'-diacetylchitobiose-specific enzyme IIB component of PTS	_____________1_________________16\n_____________\|_________________\|\n__________5'-A_________________AUAAC...CGG-3'\n______________AUGUGAAG___AGGGUC\n______________\|:\|\|\|\|\|\|___\|:\|\|\|\|\n______________UGCACUUC___UUCCAG\n3'-AUU...GCUAA________AUU______ACUUU...AUG-5'\n_____________\|_________________\|\n_____________51________________33\n	3 -- 9	43 -- 49	-17.276 kcal/mol	2.8636 kcal/mol	7.69543 kcal/mol	1 16@LB@ \| \|@LB@ 5'-A AUAAC...CGG-3'@LB@ AUGUGAAG AGGGUC@LB@ \|:\|\|\|\|\|\| \|:\|\|\|\|@LB@ UGCACUUC UUCCAG@LB@3'-AUU...GCUAA AUU ACUUU...AUG-5'@LB@ \| \|@LB@ 51 33@LB@@LB@@LB@Energy -6.64 kcal/mol@LB@Hybridization Energy -17.276 kcal/mol@LB@Unfolding Energy - mRNA 2.8636 kcal/mol@LB@Unfolding Energy - sRNA 7.69543 kcal/mol@LB@@LB@Position - mRNA 2 -- 15@LB@Position - sRNA 34 -- 50@LB@Position Seed - mRNA 3 -- 9@LB@Position Seed - sRNA 43 -- 49@LB@
75	0.001328	0.06227	b1748	astC	-8.39	0.124654	121 -- 127	1 -- 7	succinylornithine transaminase PLP-dependent	_____________120_____128\n_____________\|_______\|\n5'-UCA...CACGA_______AAUCU...UGU-3'\n______________ACCCUGC\n______________\|\|\|\|\|:\|\n______________UGGGAUG\n3'-AUU...AGACA_______-5'\n_____________\|______\|\n_____________8______1\n	121 -- 127	1 -- 7	-11.9071 kcal/mol	1.50434 kcal/mol	2.03949 kcal/mol	120 128@LB@ \| \|@LB@5'-UCA...CACGA AAUCU...UGU-3'@LB@ ACCCUGC@LB@ \|\|\|\|\|:\|@LB@ UGGGAUG@LB@3'-AUU...AGACA -5'@LB@ \| \|@LB@ 8 1@LB@@LB@@LB@Energy -8.39 kcal/mol@LB@Hybridization Energy -11.9071 kcal/mol@LB@Unfolding Energy - mRNA 1.50434 kcal/mol@LB@Unfolding Energy - sRNA 2.03949 kcal/mol@LB@@LB@Position - mRNA 121 -- 127@LB@Position - sRNA 1 -- 7@LB@Position Seed - mRNA 121 -- 127@LB@Position Seed - sRNA 1 -- 7@LB@
15	5.569e-05	0.01183	b1761	gdhA	-12.26	0.008568	280 -- 294	40 -- 56	glutamate dehydrogenase NADP-specific	_____________279_______________295\n_____________\|_________________\|\n5'-UAA...GCAAG___UU____________UGACCA-3'\n______________CCG____CGUGAAGUAA\n______________\|\|\|____\|\|\|\|\|\|\|\|\|\|\n______________GGC____GCACUUCAUU\n3'-AUU...GUUUA___UAAU__________UUCCA...AUG-5'\n_____________\|_________________\|\n_____________57________________39\n	288 -- 294	40 -- 46	-17.1153 kcal/mol	1.5283 kcal/mol	3.34823 kcal/mol	279 295@LB@ \| \|@LB@5'-UAA...GCAAG UU UGACCA-3'@LB@ CCG CGUGAAGUAA@LB@ \|\|\| \|\|\|\|\|\|\|\|\|\|@LB@ GGC GCACUUCAUU@LB@3'-AUU...GUUUA UAAU UUCCA...AUG-5'@LB@ \| \|@LB@ 57 39@LB@@LB@@LB@Energy -12.26 kcal/mol@LB@Hybridization Energy -17.1153 kcal/mol@LB@Unfolding Energy - mRNA 1.5283 kcal/mol@LB@Unfolding Energy - sRNA 3.34823 kcal/mol@LB@@LB@Position - mRNA 280 -- 294@LB@Position - sRNA 40 -- 56@LB@Position Seed - mRNA 288 -- 294@LB@Position Seed - sRNA 40 -- 46@LB@
181	0.007802	0.1516	b1797	yeaR	-7.07	0.254063	142 -- 149	2 -- 9	DUF1971 family protein nitrate-inducible	_____________141______150\n_____________\|________\|\n5'-UGC...CAGGC________AAACG...AAG-3'\n______________GUACCCUG\n______________\|\|\|\|\|\|\|:\n______________CAUGGGAU\n3'-AUU...GGAGA________G-5'\n_____________\|________\|\n_____________10_______1\n	143 -- 149	2 -- 8	-12.2602 kcal/mol	3.09143 kcal/mol	2.12684 kcal/mol	141 150@LB@ \| \|@LB@5'-UGC...CAGGC AAACG...AAG-3'@LB@ GUACCCUG@LB@ \|\|\|\|\|\|\|:@LB@ CAUGGGAU@LB@3'-AUU...GGAGA G-5'@LB@ \| \|@LB@ 10 1@LB@@LB@@LB@Energy -7.07 kcal/mol@LB@Hybridization Energy -12.2602 kcal/mol@LB@Unfolding Energy - mRNA 3.09143 kcal/mol@LB@Unfolding Energy - sRNA 2.12684 kcal/mol@LB@@LB@Position - mRNA 142 -- 149@LB@Position - sRNA 2 -- 9@LB@Position Seed - mRNA 143 -- 149@LB@Position Seed - sRNA 2 -- 8@LB@
101	0.00315	0.1097	b1822	rlmA	-7.85	0.169074	182 -- 190	1 -- 9	23S rRNA m(1)G745 methyltransferase SAM-dependent	_____________181_______191\n_____________\|_________\|\n5'-AAG...CUUUA_________CGGAG...GGU-3'\n______________GUAUCCUGC\n______________\|\|\|:\|\|\|:\|\n______________CAUGGGAUG\n3'-AUU...GGAGA_________-5'\n_____________\|________\|\n_____________10_______1\n	184 -- 190	1 -- 7	-13.1594 kcal/mol	3.20778 kcal/mol	2.12872 kcal/mol	181 191@LB@ \| \|@LB@5'-AAG...CUUUA CGGAG...GGU-3'@LB@ GUAUCCUGC@LB@ \|\|\|:\|\|\|:\|@LB@ CAUGGGAUG@LB@3'-AUU...GGAGA -5'@LB@ \| \|@LB@ 10 1@LB@@LB@@LB@Energy -7.85 kcal/mol@LB@Hybridization Energy -13.1594 kcal/mol@LB@Unfolding Energy - mRNA 3.20778 kcal/mol@LB@Unfolding Energy - sRNA 2.12872 kcal/mol@LB@@LB@Position - mRNA 182 -- 190@LB@Position - sRNA 1 -- 9@LB@Position Seed - mRNA 184 -- 190@LB@Position Seed - sRNA 1 -- 7@LB@
47	0.0005047	0.03778	b1888	cheA	-10.26	0.037926	179 -- 191	53 -- 66	fused chemotactic sensory histidine kinase in two-component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein	_____________178____________192\n_____________\|______________\|\n5'-GAU...UGCCA______________GGUGA...AAG-3'\n______________UCAGCCGAA_CCGA\n______________\|\|\|\|\|\|:\|\|_\|\|\|\|\n______________AGUCGGUUU_GGCU\n3'-AUU...UUAUA_________A____AAUGC...AUG-5'\n_____________\|______________\|\n_____________67_____________52\n	181 -- 187	58 -- 64	-19.401 kcal/mol	2.99348 kcal/mol	6.16775 kcal/mol	178 192@LB@ \| \|@LB@5'-GAU...UGCCA GGUGA...AAG-3'@LB@ UCAGCCGAA CCGA@LB@ \|\|\|\|\|\|:\|\| \|\|\|\|@LB@ AGUCGGUUU GGCU@LB@3'-AUU...UUAUA A AAUGC...AUG-5'@LB@ \| \|@LB@ 67 52@LB@@LB@@LB@Energy -10.26 kcal/mol@LB@Hybridization Energy -19.401 kcal/mol@LB@Unfolding Energy - mRNA 2.99348 kcal/mol@LB@Unfolding Energy - sRNA 6.16775 kcal/mol@LB@@LB@Position - mRNA 179 -- 191@LB@Position - sRNA 53 -- 66@LB@Position Seed - mRNA 181 -- 187@LB@Position Seed - sRNA 58 -- 64@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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